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The map label for this gene is galK

Identifier: 16080871

GI number: 16080871

Start: 3920638

End: 3921810

Strand: Reverse

Name: galK

Synonym: BSU38200

Alternate gene names: 16080871

Gene position: 3921810-3920638 (Counterclockwise)

Preceding gene: 16080872

Following gene: 255767809

Centisome position: 93.03

GC content: 46.12

Gene sequence:

>1173_bases
ATGCGGGAAGAGCTAAAGGGGATATTTGCAAGTGTATTCGGAGAAAAAGAGGGGTTACGGTTTTTTTTCGCACCTGGAAG
AGTGAATCTGATTGGAGAACATACAGACTATAACGGCGGGCATGTCTTTCCGTGCGCGCTGACAATGGGTACATATGCTG
CTGTTGCAGAAAGAAACGACGGGCTTGTCCGGATGTATTCAGATAACTTTAGAAACGCAGGCATCAAGGAATGCAGCCTT
GACGACATTCGCTATCAAAAAGAAGACGACTGGGCGAACTACCCAAAAGGCGTGATTTATGAATTTCAACAGAGGGGATA
TGCAGTTCCCCATGGCTTTGACATTGTATTTTCAGGGAATATTCCAAACGGGGCGGGACTGTCGTCTTCCGCTTCTATTG
AACTGCTGATGGGGGTCGTGCTCCAAAGTTATTTTCATCCTGAAGTAGATGCGCTTGAGCTTGTGAAAATGGCGCAGCAT
GCGGAAAACCATTTTATCGGCGTCAATTGCGGAATTATGGACCAATTTGCAATTGGAATGGGGAAAAAACATCATGCGAT
GCTGCTGAACTGTGACACGCTTGACTATGAATATAGCAAACTCAATGTCTCAGGCCTCGCGCTGGTCATCGCGAACACGA
ACAAAAAAAGGACATTGGCTGACTCCAGCTATAATACGAGACGCCAAGAATGCAATGATGCATTGCTTGATCTGAAAAAA
GGCCTCGATATTGCCGCACTGGGCGACATCAAGCCTTCTGATTTCGATGCGCATTCCAGCCTGATCCAAAATGAAACGAA
CAGACGCCGGGCAAAGCACGCTGTATACGAAAATCATCGTGCTATTAAGACGGCCCATATGTTCAAAGAGAACAATATAG
ATGAAATCGGGCAGTTAATGAAGGAATCCCACCTTTCATTGAAGGATGACTATGAAGTAACATGCCCTGAACTTGATGAG
CTTGTGTTTGCGGCATGGGATCACGAAGGTGTAATTGGATCAAGAATGACGGGAGCAGGCTTTGGCGGCTGCACCATCAG
CATTGTAAAAGATGAGTTCGTTGATGATTTTATTCAGAAGGTTGGAGACAGATATCAAGAAAAAACAGGGCTCCGAGCGG
ACTTTTATGTCGCGGATATCGGAGAGGGAGCGAGAGAACTAAAGGGGGAGTGA

Upstream 100 bases:

>100_bases
CAAAAGAAGAAGGGGTATGAGAGGACTGCAGCATATGATCTGCCGTTTTCTATGAAGAAATAAGGGGAATAAAAAATAAA
AACCAGAAAGGTGATTGCAC

Downstream 100 bases:

>100_bases
CATATGAGTATTATTGAACATCTGGAACAGCTGCTTGAGTATGGCCTGGAACGAAAATTGATTTCGATCTGGGATCGGGA
ATATACGAGAAACCGTCTTT

Product: galactokinase

Products: NA

Alternate protein names: Galactose kinase

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MREELKGIFASVFGEKEGLRFFFAPGRVNLIGEHTDYNGGHVFPCALTMGTYAAVAERNDGLVRMYSDNFRNAGIKECSL
DDIRYQKEDDWANYPKGVIYEFQQRGYAVPHGFDIVFSGNIPNGAGLSSSASIELLMGVVLQSYFHPEVDALELVKMAQH
AENHFIGVNCGIMDQFAIGMGKKHHAMLLNCDTLDYEYSKLNVSGLALVIANTNKKRTLADSSYNTRRQECNDALLDLKK
GLDIAALGDIKPSDFDAHSSLIQNETNRRRAKHAVYENHRAIKTAHMFKENNIDEIGQLMKESHLSLKDDYEVTCPELDE
LVFAAWDHEGVIGSRMTGAGFGGCTISIVKDEFVDDFIQKVGDRYQEKTGLRADFYVADIGEGARELKGE

Sequences:

>Translated_390_residues
MREELKGIFASVFGEKEGLRFFFAPGRVNLIGEHTDYNGGHVFPCALTMGTYAAVAERNDGLVRMYSDNFRNAGIKECSL
DDIRYQKEDDWANYPKGVIYEFQQRGYAVPHGFDIVFSGNIPNGAGLSSSASIELLMGVVLQSYFHPEVDALELVKMAQH
AENHFIGVNCGIMDQFAIGMGKKHHAMLLNCDTLDYEYSKLNVSGLALVIANTNKKRTLADSSYNTRRQECNDALLDLKK
GLDIAALGDIKPSDFDAHSSLIQNETNRRRAKHAVYENHRAIKTAHMFKENNIDEIGQLMKESHLSLKDDYEVTCPELDE
LVFAAWDHEGVIGSRMTGAGFGGCTISIVKDEFVDDFIQKVGDRYQEKTGLRADFYVADIGEGARELKGE
>Mature_390_residues
MREELKGIFASVFGEKEGLRFFFAPGRVNLIGEHTDYNGGHVFPCALTMGTYAAVAERNDGLVRMYSDNFRNAGIKECSL
DDIRYQKEDDWANYPKGVIYEFQQRGYAVPHGFDIVFSGNIPNGAGLSSSASIELLMGVVLQSYFHPEVDALELVKMAQH
AENHFIGVNCGIMDQFAIGMGKKHHAMLLNCDTLDYEYSKLNVSGLALVIANTNKKRTLADSSYNTRRQECNDALLDLKK
GLDIAALGDIKPSDFDAHSSLIQNETNRRRAKHAVYENHRAIKTAHMFKENNIDEIGQLMKESHLSLKDDYEVTCPELDE
LVFAAWDHEGVIGSRMTGAGFGGCTISIVKDEFVDDFIQKVGDRYQEKTGLRADFYVADIGEGARELKGE

Specific function: Galactose metabolism; first step. [C]

COG id: COG0153

COG function: function code G; Galactokinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. GalK subfamily

Homologues:

Organism=Homo sapiens, GI4503895, Length=390, Percent_Identity=37.9487179487179, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI4503897, Length=425, Percent_Identity=30.1176470588235, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI48527957, Length=425, Percent_Identity=30.1176470588235, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1786972, Length=388, Percent_Identity=40.4639175257732, Blast_Score=291, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI71989053, Length=423, Percent_Identity=25.7683215130024, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6319494, Length=237, Percent_Identity=30.8016877637131, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320212, Length=280, Percent_Identity=27.8571428571429, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24661292, Length=432, Percent_Identity=26.6203703703704, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355577, Length=432, Percent_Identity=26.6203703703704, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24661285, Length=432, Percent_Identity=26.6203703703704, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GAL1_BACSU (P39574)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39690
- RefSeq:   NP_391699.1
- ProteinModelPortal:   P39574
- SMR:   P39574
- EnsemblBacteria:   EBBACT00000001310
- GeneID:   937297
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38200
- NMPDR:   fig|224308.1.peg.3825
- GenoList:   BSU38200
- GeneTree:   EBGT00050000001035
- HOGENOM:   HBG725121
- OMA:   EYVGAPT
- PhylomeDB:   P39574
- ProtClustDB:   PRK05322
- BioCyc:   BSUB:BSU38200-MONOMER
- BRENDA:   2.7.1.6
- GO:   GO:0005737
- HAMAP:   MF_00246
- InterPro:   IPR000705
- InterPro:   IPR022963
- InterPro:   IPR019741
- InterPro:   IPR019539
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR006206
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000530
- PRINTS:   PR00473
- PRINTS:   PR00959
- TIGRFAMs:   TIGR00131

Pfam domain/function: PF10509 GalKase_gal_bdg; PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.6

Molecular weight: Translated: 43522; Mature: 43522

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00106 GALACTOKINASE; PS00627 GHMP_KINASES_ATP; PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREELKGIFASVFGEKEGLRFFFAPGRVNLIGEHTDYNGGHVFPCALTMGTYAAVAERND
CCHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCCEEEEEHHHHHHHHHEEECCC
GLVRMYSDNFRNAGIKECSLDDIRYQKEDDWANYPKGVIYEFQQRGYAVPHGFDIVFSGN
CEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECC
IPNGAGLSSSASIELLMGVVLQSYFHPEVDALELVKMAQHAENHFIGVNCGIMDQFAIGM
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCC
GKKHHAMLLNCDTLDYEYSKLNVSGLALVIANTNKKRTLADSSYNTRRQECNDALLDLKK
CCCCCEEEEECCCCCCCCCEECCCEEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHH
GLDIAALGDIKPSDFDAHSSLIQNETNRRRAKHAVYENHRAIKTAHMFKENNIDEIGQLM
CCCEEEECCCCCCCCCHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHH
KESHLSLKDDYEVTCPELDELVFAAWDHEGVIGSRMTGAGFGGCTISIVKDEFVDDFIQK
HHHCCCCCCCCEECCCCHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHH
VGDRYQEKTGLRADFYVADIGEGARELKGE
HHHHHHHHCCCCEEEEEEECCCCHHHHCCC
>Mature Secondary Structure
MREELKGIFASVFGEKEGLRFFFAPGRVNLIGEHTDYNGGHVFPCALTMGTYAAVAERND
CCHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCCEEEEEHHHHHHHHHEEECCC
GLVRMYSDNFRNAGIKECSLDDIRYQKEDDWANYPKGVIYEFQQRGYAVPHGFDIVFSGN
CEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECC
IPNGAGLSSSASIELLMGVVLQSYFHPEVDALELVKMAQHAENHFIGVNCGIMDQFAIGM
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCC
GKKHHAMLLNCDTLDYEYSKLNVSGLALVIANTNKKRTLADSSYNTRRQECNDALLDLKK
CCCCCEEEEECCCCCCCCCEECCCEEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHH
GLDIAALGDIKPSDFDAHSSLIQNETNRRRAKHAVYENHRAIKTAHMFKENNIDEIGQLM
CCCEEEECCCCCCCCCHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHH
KESHLSLKDDYEVTCPELDELVFAAWDHEGVIGSRMTGAGFGGCTISIVKDEFVDDFIQK
HHHCCCCCCCCEECCCCHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHH
VGDRYQEKTGLRADFYVADIGEGARELKGE
HHHHHHHHCCCCEEEEEEECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377