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The map label for this gene is ywcJ

Identifier: 16080857

GI number: 16080857

Start: 3905333

End: 3906103

Strand: Direct

Name: ywcJ

Synonym: BSU38060

Alternate gene names: 16080857

Gene position: 3905333-3906103 (Clockwise)

Preceding gene: 16080853

Following gene: 16080860

Centisome position: 92.64

GC content: 45.65

Gene sequence:

>771_bases
ATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTA
TGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTA
TGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGA
GATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTT
GTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGG
AGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGA
ATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCAT
GATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTT
TGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCG
GGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAA

Upstream 100 bases:

>100_bases
GATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATG
AAAGAAAGCAGGAAAAGACA

Downstream 100 bases:

>100_bases
AAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGAT
ATAGCCGGCCTCGGATTCCG

Product: formate/nitrite transporter

Products: Proton [Cytoplasm]; nitrite [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
METQALQKVEQYALKKQNIFASSKIRYVLRSILASIFIGFGITAASKTGSYFFMADSPFAFPAAAVTFGAAILMIAYGGG
DLFTGNTFYFTYTALRKKISWRDTLYLWMSSYAGNLIGAILFAILISATGLFEEPSVHSFLIHLAEHKMEPPASELFFRG
MLCNWLVCLAFFIPMSLKGEGAKLFTMMLFVFCFFISGFEHSIANMCTFAISLLIEHPDTVTLMGAVRNLIPVTLGNLTA
GIVMMGWMYYTLNPDQ

Sequences:

>Translated_256_residues
METQALQKVEQYALKKQNIFASSKIRYVLRSILASIFIGFGITAASKTGSYFFMADSPFAFPAAAVTFGAAILMIAYGGG
DLFTGNTFYFTYTALRKKISWRDTLYLWMSSYAGNLIGAILFAILISATGLFEEPSVHSFLIHLAEHKMEPPASELFFRG
MLCNWLVCLAFFIPMSLKGEGAKLFTMMLFVFCFFISGFEHSIANMCTFAISLLIEHPDTVTLMGAVRNLIPVTLGNLTA
GIVMMGWMYYTLNPDQ
>Mature_256_residues
METQALQKVEQYALKKQNIFASSKIRYVLRSILASIFIGFGITAASKTGSYFFMADSPFAFPAAAVTFGAAILMIAYGGG
DLFTGNTFYFTYTALRKKISWRDTLYLWMSSYAGNLIGAILFAILISATGLFEEPSVHSFLIHLAEHKMEPPASELFFRG
MLCNWLVCLAFFIPMSLKGEGAKLFTMMLFVFCFFISGFEHSIANMCTFAISLLIEHPDTVTLMGAVRNLIPVTLGNLTA
GIVMMGWMYYTLNPDQ

Specific function: Potential Nitrite Transporter. [C]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family

Homologues:

Organism=Escherichia coli, GI87082249, Length=256, Percent_Identity=35.15625, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787132, Length=275, Percent_Identity=29.4545454545455, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788837, Length=273, Percent_Identity=27.8388278388278, Blast_Score=79, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321779, Length=193, Percent_Identity=24.8704663212435, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YWCJ_BACSU (P39608)

Other databases:

- EMBL:   J03006
- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39703
- RefSeq:   NP_391685.1
- ProteinModelPortal:   P39608
- SMR:   P39608
- EnsemblBacteria:   EBBACT00000001992
- GeneID:   937283
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38060
- NMPDR:   fig|224308.1.peg.3811
- GenoList:   BSU38060
- GeneTree:   EBGT00050000001469
- HOGENOM:   HBG726755
- OMA:   YCTFFNL
- PhylomeDB:   P39608
- ProtClustDB:   CLSK2518288
- BioCyc:   BSUB:BSU38060-MONOMER
- InterPro:   IPR000292
- TIGRFAMs:   TIGR00790

Pfam domain/function: PF01226 Form_Nir_trans

EC number: NA

Molecular weight: Translated: 28461; Mature: 28461

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS01005 FORMATE_NITRITE_TP_1; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x126e4464)-; HASH(0x1284ed7c)-; HASH(0x129bc8cc)-; HASH(0x11e2d6a4)-; HASH(0x120ae244)-; HASH(0x12bbd1bc)-; HASH(0x1188c68c)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METQALQKVEQYALKKQNIFASSKIRYVLRSILASIFIGFGITAASKTGSYFFMADSPFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
FPAAAVTFGAAILMIAYGGGDLFTGNTFYFTYTALRKKISWRDTLYLWMSSYAGNLIGAI
CHHHHHHHHHHHHHEEECCCCEEECCEEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
LFAILISATGLFEEPSVHSFLIHLAEHKMEPPASELFFRGMLCNWLVCLAFFIPMSLKGE
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GAKLFTMMLFVFCFFISGFEHSIANMCTFAISLLIEHPDTVTLMGAVRNLIPVTLGNLTA
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GIVMMGWMYYTLNPDQ
HHHHHHHEEEEECCCH
>Mature Secondary Structure
METQALQKVEQYALKKQNIFASSKIRYVLRSILASIFIGFGITAASKTGSYFFMADSPFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
FPAAAVTFGAAILMIAYGGGDLFTGNTFYFTYTALRKKISWRDTLYLWMSSYAGNLIGAI
CHHHHHHHHHHHHHEEECCCCEEECCEEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
LFAILISATGLFEEPSVHSFLIHLAEHKMEPPASELFFRGMLCNWLVCLAFFIPMSLKGE
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GAKLFTMMLFVFCFFISGFEHSIANMCTFAISLLIEHPDTVTLMGAVRNLIPVTLGNLTA
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GIVMMGWMYYTLNPDQ
HHHHHHHEEEEECCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; nitrite [Periplasm] [C]

Specific reaction: Proton [Periplasm] + nitrite [Periplasm] = Proton [Cytoplasm] + nitrite [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2163394; 7934828; 9384377