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The map label for this gene is thiD

Identifier: 16080853

GI number: 16080853

Start: 3900963

End: 3901778

Strand: Direct

Name: thiD

Synonym: BSU38020

Alternate gene names: 16080853

Gene position: 3900963-3901778 (Clockwise)

Preceding gene: 255767801

Following gene: 16080857

Centisome position: 92.54

GC content: 48.16

Gene sequence:

>816_bases
ATGTCTATGCATAAAGCACTCACCATTGCCGGCTCAGATTCCAGCGGCGGTGCTGGGATTCAAGCTGATTTAAAAACATT
TCAAGAAAAAAACGTATACGGGATGACCGCCTTAACGGTGATCGTTGCGATGGACCCAAACAACAGCTGGAACCATCAGG
TATTCCCGATCGATACTGATACAATCCGCGCCCAGCTTGCAACAATTACGGACGGCATCGGCGTGGACGCCATGAAAACA
GGAATGCTGCCAACCGTTGACATTATCGAGCTTGCGGCAAAAACGATCAAAGAAAAACAATTAAAAAATGTGGTAATTGA
CCCGGTTATGGTTTGCAAAGGTGCTAATGAAGTCCTTTATCCGGAGCACGCCCAAGCCCTGCGTGAGCAATTGGCTCCGC
TAGCCACTGTCATTACGCCAAACTTGTTTGAAGCCAGCCAGCTCAGCGGCATGGATGAACTAAAAACAGTTGACGATATG
ATCGAAGCCGCGAAAAAAATTCACGCACTCGGCGCTCAATATGTCGTCATTACAGGCGGAGGCAAACTCAAACACGAGAA
AGCCGTTGATGTTCTCTATGACGGAGAAACAGCGGAAGTCCTTGAAAGCGAAATGATCGATACGCCTTATACACATGGCG
CAGGCTGTACATTCTCAGCAGCCGTGACAGCGGAGCTTGCCAAAGGTGCGGAAGTCAAAGAAGCCATTTACGCAGCGAAA
GAATTCATCACAGCGGCCATTAAAGAATCCTTCCCGCTAAACCAGTATGTTGGTCCTACGAAGCATTCTGCACTGCGCCT
GAATCAACAATCATAA

Upstream 100 bases:

>100_bases
GCCCGTTGAGATTGTCAAATGATTCCTATAGATCTTCTATATTGTGATACAGAGAAAATCTAACTATAATGAAAGACAAA
ACATGATGGAGGTTCAAGAC

Downstream 100 bases:

>100_bases
AAAAAGGGGCTGCCTTACAGAGAGGCAGCCCTTTTTAAATCACCGAAGACTTTTCACAGAAAAAACTGCCGATCCCATCG
GCAGCCTGTTTATTTGATTT

Product: pyridoxal kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSMHKALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVIVAMDPNNSWNHQVFPIDTDTIRAQLATITDGIGVDAMKT
GMLPTVDIIELAAKTIKEKQLKNVVIDPVMVCKGANEVLYPEHAQALREQLAPLATVITPNLFEASQLSGMDELKTVDDM
IEAAKKIHALGAQYVVITGGGKLKHEKAVDVLYDGETAEVLESEMIDTPYTHGAGCTFSAAVTAELAKGAEVKEAIYAAK
EFITAAIKESFPLNQYVGPTKHSALRLNQQS

Sequences:

>Translated_271_residues
MSMHKALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVIVAMDPNNSWNHQVFPIDTDTIRAQLATITDGIGVDAMKT
GMLPTVDIIELAAKTIKEKQLKNVVIDPVMVCKGANEVLYPEHAQALREQLAPLATVITPNLFEASQLSGMDELKTVDDM
IEAAKKIHALGAQYVVITGGGKLKHEKAVDVLYDGETAEVLESEMIDTPYTHGAGCTFSAAVTAELAKGAEVKEAIYAAK
EFITAAIKESFPLNQYVGPTKHSALRLNQQS
>Mature_270_residues
SMHKALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVIVAMDPNNSWNHQVFPIDTDTIRAQLATITDGIGVDAMKTG
MLPTVDIIELAAKTIKEKQLKNVVIDPVMVCKGANEVLYPEHAQALREQLAPLATVITPNLFEASQLSGMDELKTVDDMI
EAAKKIHALGAQYVVITGGGKLKHEKAVDVLYDGETAEVLESEMIDTPYTHGAGCTFSAAVTAELAKGAEVKEAIYAAKE
FITAAIKESFPLNQYVGPTKHSALRLNQQS

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates. Can also use hydroxymethylpyrimidine (HMP) as substrate

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family

Homologues:

Organism=Escherichia coli, GI1788420, Length=264, Percent_Identity=35.9848484848485, Blast_Score=165, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6325378, Length=275, Percent_Identity=30.1818181818182, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324997, Length=254, Percent_Identity=30.7086614173228, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324517, Length=256, Percent_Identity=28.90625, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXK_BACSU (P39610)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39707
- RefSeq:   NP_391681.1
- PDB:   2I5B
- PDBsum:   2I5B
- ProteinModelPortal:   P39610
- SMR:   P39610
- EnsemblBacteria:   EBBACT00000000115
- GeneID:   937262
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38020
- NMPDR:   fig|224308.1.peg.3807
- GenoList:   BSU38020
- GeneTree:   EBGT00050000001225
- HOGENOM:   HBG476673
- OMA:   NPGNTDA
- PhylomeDB:   P39610
- ProtClustDB:   PRK12616
- BioCyc:   BSUB:BSU38020-MONOMER
- BRENDA:   2.7.4.7
- InterPro:   IPR013749
- InterPro:   IPR004399
- TIGRFAMs:   TIGR00097

Pfam domain/function: PF08543 Phos_pyr_kin

EC number: =2.7.1.35

Molecular weight: Translated: 29018; Mature: 28886

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: BINDING 141-141 BINDING 190-190 BINDING 206-206 BINDING 215-215 BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMHKALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVIVAMDPNNSWNHQVFPIDTD
CCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEEEECCHH
TIRAQLATITDGIGVDAMKTGMLPTVDIIELAAKTIKEKQLKNVVIDPVMVCKGANEVLY
HHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCCCC
PEHAQALREQLAPLATVITPNLFEASQLSGMDELKTVDDMIEAAKKIHALGAQYVVITGG
CHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GKLKHEKAVDVLYDGETAEVLESEMIDTPYTHGAGCTFSAAVTAELAKGAEVKEAIYAAK
CCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHH
EFITAAIKESFPLNQYVGPTKHSALRLNQQS
HHHHHHHHHCCCCHHCCCCCCCCEEECCCCC
>Mature Secondary Structure 
SMHKALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVIVAMDPNNSWNHQVFPIDTD
CCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEEEECCHH
TIRAQLATITDGIGVDAMKTGMLPTVDIIELAAKTIKEKQLKNVVIDPVMVCKGANEVLY
HHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCCCC
PEHAQALREQLAPLATVITPNLFEASQLSGMDELKTVDDMIEAAKKIHALGAQYVVITGG
CHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GKLKHEKAVDVLYDGETAEVLESEMIDTPYTHGAGCTFSAAVTAELAKGAEVKEAIYAAK
CCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHH
EFITAAIKESFPLNQYVGPTKHSALRLNQQS
HHHHHHHHHCCCCHHCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377