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The map label for this gene is narI

Identifier: 16080778

GI number: 16080778

Start: 3823558

End: 3824229

Strand: Reverse

Name: narI

Synonym: BSU37250

Alternate gene names: 16080778

Gene position: 3824229-3823558 (Counterclockwise)

Preceding gene: 16080779

Following gene: 16080777

Centisome position: 90.72

GC content: 50.15

Gene sequence:

>672_bases
ATGAGCGGGCAGATCCTCTGGGGTATTATGCCATACATTGTATTGACAATCTTTATCGGCGGCCATATTTACCGCTATCA
GCATGACCAATTTGGCTGGACGGCGAAATCAAGCGAGCTGTTAGAAAAGAAAAAACTTGCGGCTGGCAGCACACTTTTTC
ACTGGGGACTGCTGTGCGTTGTCGGCGGGCATGTCATGGGGATTCTGATCCCAGAAGGCGTGTATGCTTCCCTTGGCATT
TCAGAGCATATGTATCACAAAATGGCGATTGGCGCTGGCTTGCCGGCGGGCATTGCGGCATGTACCGGACTTGTCATCCT
GACGTACAGAAGGCTGTTTGACAAAAGAATCCGCAAAACGAGCTCGCCATCCGATATCCTTACGCTCCTCCTGCTGCTGT
TCATGATGCTGTCAGGCGTTGCGGCCACGTTTCTCAACATTGATTCGAAAGGATTTGATTACCGGACCACAGTCGGGCCC
TGGTTCAGGGAAATCGTTTTGTTCAGGCCTGACGCCTCTTTGATGGAGAGTGTCCCGCTATGGTTTAAGTTTCATATTGT
GATAGGATACGTCGTTTTTATCCTGTGGCCGTTTACGAGATTGGTTCATGTGTTCAGTCTGCCGCTCAAGTATCTGACCC
GCAGCTACGTTGTATATCGGAAACGCTCGTGA

Upstream 100 bases:

>100_bases
TCGAAAAAAATGACAGTATATACGCTGAACTGCTGCACGTGCTGCTGGCCGCGCTTGAAAACATTGGCGTACGTGAAAGC
GTTGAAGGGGCTGTTCAGGC

Downstream 100 bases:

>100_bases
AAATCCCCCTTGCAAGAGGGGGTTTTTTCTGCCAAGGTAATGGCTGGGCTGCCGGGAATCCTGTTATGTTTGTATATGGA
AATAACATCAAGGATTTTAC

Product: nitrate reductase gamma subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSGQILWGIMPYIVLTIFIGGHIYRYQHDQFGWTAKSSELLEKKKLAAGSTLFHWGLLCVVGGHVMGILIPEGVYASLGI
SEHMYHKMAIGAGLPAGIAACTGLVILTYRRLFDKRIRKTSSPSDILTLLLLLFMMLSGVAATFLNIDSKGFDYRTTVGP
WFREIVLFRPDASLMESVPLWFKFHIVIGYVVFILWPFTRLVHVFSLPLKYLTRSYVVYRKRS

Sequences:

>Translated_223_residues
MSGQILWGIMPYIVLTIFIGGHIYRYQHDQFGWTAKSSELLEKKKLAAGSTLFHWGLLCVVGGHVMGILIPEGVYASLGI
SEHMYHKMAIGAGLPAGIAACTGLVILTYRRLFDKRIRKTSSPSDILTLLLLLFMMLSGVAATFLNIDSKGFDYRTTVGP
WFREIVLFRPDASLMESVPLWFKFHIVIGYVVFILWPFTRLVHVFSLPLKYLTRSYVVYRKRS
>Mature_222_residues
SGQILWGIMPYIVLTIFIGGHIYRYQHDQFGWTAKSSELLEKKKLAAGSTLFHWGLLCVVGGHVMGILIPEGVYASLGIS
EHMYHKMAIGAGLPAGIAACTGLVILTYRRLFDKRIRKTSSPSDILTLLLLLFMMLSGVAATFLNIDSKGFDYRTTVGPW
FREIVLFRPDASLMESVPLWFKFHIVIGYVVFILWPFTRLVHVFSLPLKYLTRSYVVYRKRS

Specific function: The Nitrate Reductase Enzyme Complex Allows E.Coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Gamma Chain Is A Membrane-Embedded Heme-Iron Unit Resembling Cytochrome B, Which Transfers Electrons From Quinones To The Beta Subunit.

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=216, Percent_Identity=37.037037037037, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1787738, Length=217, Percent_Identity=37.3271889400922, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NARI_BACSU (P42177)

Other databases:

- EMBL:   Z49884
- EMBL:   AL009126
- EMBL:   X85014
- EMBL:   X91819
- PIR:   S60088
- RefSeq:   NP_391606.1
- ProteinModelPortal:   P42177
- SMR:   P42177
- EnsemblBacteria:   EBBACT00000003135
- GeneID:   938454
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37250
- NMPDR:   fig|224308.1.peg.3732
- GenoList:   BSU37250
- GeneTree:   EBGT00050000002898
- HOGENOM:   HBG308661
- OMA:   MVSGGFF
- PhylomeDB:   P42177
- ProtClustDB:   CLSK888055
- BioCyc:   BSUB:BSU37250-MONOMER
- BRENDA:   1.7.99.4
- GO:   GO:0006810
- InterPro:   IPR003816
- TIGRFAMs:   TIGR00351

Pfam domain/function: PF02665 Nitrate_red_gam

EC number: =1.7.99.4

Molecular weight: Translated: 25297; Mature: 25165

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12e69ae4)-; HASH(0x133eed24)-; HASH(0x133eeee0)-; HASH(0x1340cb80)-; HASH(0x133e6240)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQILWGIMPYIVLTIFIGGHIYRYQHDQFGWTAKSSELLEKKKLAAGSTLFHWGLLCV
CCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VGGHVMGILIPEGVYASLGISEHMYHKMAIGAGLPAGIAACTGLVILTYRRLFDKRIRKT
HHHHHHEEEECCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SSPSDILTLLLLLFMMLSGVAATFLNIDSKGFDYRTTVGPWFREIVLFRPDASLMESVPL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHEEECCCHHHHHCCCH
WFKFHIVIGYVVFILWPFTRLVHVFSLPLKYLTRSYVVYRKRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCH
>Mature Secondary Structure 
SGQILWGIMPYIVLTIFIGGHIYRYQHDQFGWTAKSSELLEKKKLAAGSTLFHWGLLCV
CCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VGGHVMGILIPEGVYASLGISEHMYHKMAIGAGLPAGIAACTGLVILTYRRLFDKRIRKT
HHHHHHEEEECCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SSPSDILTLLLLLFMMLSGVAATFLNIDSKGFDYRTTVGPWFREIVLFRPDASLMESVPL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHEEECCCHHHHHCCCH
WFKFHIVIGYVVFILWPFTRLVHVFSLPLKYLTRSYVVYRKRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377; 7557333