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The map label for this gene is ywiE

Identifier: 16080777

GI number: 16080777

Start: 3821570

End: 3823072

Strand: Reverse

Name: ywiE

Synonym: BSU37240

Alternate gene names: 16080777

Gene position: 3823072-3821570 (Counterclockwise)

Preceding gene: 16080778

Following gene: 16080776

Centisome position: 90.69

GC content: 45.31

Gene sequence:

>1503_bases
ATGCTGAAAAGGAGACTGGAATTTTTCTTTTTATATATGATGCTCATCGGGGCATATGTCATTTGGTTTTTTCCGGTTTC
CAGACTTGAGTTTTACGGAGGATTGCTTTGCTATATAAGCATTATTTTGTTCAGCATCTATTCATTAATTCTTGAAAACC
GCACCTCGCAGCACACGCTGCTATGGATCCATATTCTTGTCTTTTTTCCAATCGTCGGCTACGTGTTTTATCTGTTTTCG
GGACAGCTTTATGTAAAGGGAAAGCTGTTTAAGACAAAACGAATGTACAACAGGGAAAAGCTCAGGAAGCTGTTTGACAA
GGAGGAAACGCCCGAAGTGACAGGGCTGAAGGACAATCAAGAGAGGTTCTTTACATATTCCATCAGGGCTGCCCATATGA
ATATCAATACGAAAAGCAACATCAAAGTGCTGAAAAACGGCGAAGAAACCTTCCCGGACATTTTCAAAGCAATGAGAAAA
GCGGAATCTTACATACATATTGAATACTACATGTTTAAATCAGACATGCTCGGACGCGGCATGATGGATATTATGATGGA
GAAAGCGCGCCAGGGTGTCGAGGTGAGGTTTTTATACGATGCGGCAGGAAGCATGAAGCTGGCAAGAAGAGATATTATGA
GAATGAAACAAGCGGGGGTGGACATCGTCCCGTTTTCTCCGCTGAAATACGGGTTTTTCAACCAGAAGCTGAATTTCAGA
AATCACCGGAAAATCGTGATCATCGATGGGAAAACCGGTTTTGTGGGTGGATTAAATGTCGGGAAGGAATATATCAGCAG
AGATCCGTACATCGGATTTTGGAGAGATACACACCTGCGGCTTGAGGGGGAAATTGTGCAAACGCTCCACGCTATCTTTA
TGCTGGATTGGGAATATGTCTCCAATGAAGTGCTAATTGATCAAGAGGAGTACAATACCCCGGTTCCCGTGGAAGGAGGA
GGCATTTATCAAATTGTCGCGACAGGCCCTGACATGAAGGAAAGCATGAGCGACCTTTATTATGAAATGATTTCTTCAGC
GCAGAAATCAATATGGATCGCCACGCCCTATTTTGTCCCCAATGAATCCATTCGCACGGCGCTAAAAGCAGCGGCGACAA
AGGGGGTAGAGGTAAGGGTTATGGTGCCGGAGAAGAACGACAGCTTTTTGACCCAATACGCGTCGAGGTCCTATTTCCCG
GAGCTGCTCCTTGAGGGCATTGAAGTGTACTCCTACCAAAAAGGCTTCATGCATCAAAAAGTCATGATCATTGATGGCGA
TCTCGCCTCCGTCGGTACCGCGAATATGGACATGCGCAGCTTTCAGCTCAACTTTGAAGTGAATGTGTTCTTCACGGATG
CCGAAGCGATCCGAACCCTTGAGGCCCATTTTGAAGAGGATATGCAGGAAAGTGAAAAGCTCAGCCCTGTCGGCTTTTAT
AAAAGAGGTGTGGCTGACCGGACAAAAGAGTCGTTTGCCCGCCTGTTTTCAGGTGTTCTGTAA

Upstream 100 bases:

>100_bases
CCTCTCCTCCTTTGGCGGAGAGGTTTTTTACTTACAAGGTTTATCGATTAGAAAAAAGAGGTAATACAGAGGTAATTGAA
TCAGATTGGAGAGAATCAGG

Downstream 100 bases:

>100_bases
GAAAAAAGAAATCTGATCTTACAAAACAGCCCGTGTATAGTATAATTGAGAAATATTATCAGTTATTTATACATTGGGGG
AAGATAGGGTGCTGCGGCAA

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase 1

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MLKRRLEFFFLYMMLIGAYVIWFFPVSRLEFYGGLLCYISIILFSIYSLILENRTSQHTLLWIHILVFFPIVGYVFYLFS
GQLYVKGKLFKTKRMYNREKLRKLFDKEETPEVTGLKDNQERFFTYSIRAAHMNINTKSNIKVLKNGEETFPDIFKAMRK
AESYIHIEYYMFKSDMLGRGMMDIMMEKARQGVEVRFLYDAAGSMKLARRDIMRMKQAGVDIVPFSPLKYGFFNQKLNFR
NHRKIVIIDGKTGFVGGLNVGKEYISRDPYIGFWRDTHLRLEGEIVQTLHAIFMLDWEYVSNEVLIDQEEYNTPVPVEGG
GIYQIVATGPDMKESMSDLYYEMISSAQKSIWIATPYFVPNESIRTALKAAATKGVEVRVMVPEKNDSFLTQYASRSYFP
ELLLEGIEVYSYQKGFMHQKVMIIDGDLASVGTANMDMRSFQLNFEVNVFFTDAEAIRTLEAHFEEDMQESEKLSPVGFY
KRGVADRTKESFARLFSGVL

Sequences:

>Translated_500_residues
MLKRRLEFFFLYMMLIGAYVIWFFPVSRLEFYGGLLCYISIILFSIYSLILENRTSQHTLLWIHILVFFPIVGYVFYLFS
GQLYVKGKLFKTKRMYNREKLRKLFDKEETPEVTGLKDNQERFFTYSIRAAHMNINTKSNIKVLKNGEETFPDIFKAMRK
AESYIHIEYYMFKSDMLGRGMMDIMMEKARQGVEVRFLYDAAGSMKLARRDIMRMKQAGVDIVPFSPLKYGFFNQKLNFR
NHRKIVIIDGKTGFVGGLNVGKEYISRDPYIGFWRDTHLRLEGEIVQTLHAIFMLDWEYVSNEVLIDQEEYNTPVPVEGG
GIYQIVATGPDMKESMSDLYYEMISSAQKSIWIATPYFVPNESIRTALKAAATKGVEVRVMVPEKNDSFLTQYASRSYFP
ELLLEGIEVYSYQKGFMHQKVMIIDGDLASVGTANMDMRSFQLNFEVNVFFTDAEAIRTLEAHFEEDMQESEKLSPVGFY
KRGVADRTKESFARLFSGVL
>Mature_500_residues
MLKRRLEFFFLYMMLIGAYVIWFFPVSRLEFYGGLLCYISIILFSIYSLILENRTSQHTLLWIHILVFFPIVGYVFYLFS
GQLYVKGKLFKTKRMYNREKLRKLFDKEETPEVTGLKDNQERFFTYSIRAAHMNINTKSNIKVLKNGEETFPDIFKAMRK
AESYIHIEYYMFKSDMLGRGMMDIMMEKARQGVEVRFLYDAAGSMKLARRDIMRMKQAGVDIVPFSPLKYGFFNQKLNFR
NHRKIVIIDGKTGFVGGLNVGKEYISRDPYIGFWRDTHLRLEGEIVQTLHAIFMLDWEYVSNEVLIDQEEYNTPVPVEGG
GIYQIVATGPDMKESMSDLYYEMISSAQKSIWIATPYFVPNESIRTALKAAATKGVEVRVMVPEKNDSFLTQYASRSYFP
ELLLEGIEVYSYQKGFMHQKVMIIDGDLASVGTANMDMRSFQLNFEVNVFFTDAEAIRTLEAHFEEDMQESEKLSPVGFY
KRGVADRTKESFARLFSGVL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=480, Percent_Identity=26.6666666666667, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1787007, Length=336, Percent_Identity=29.4642857142857, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLS1_BACSU (P45860)

Other databases:

- EMBL:   Z49884
- EMBL:   Z49782
- EMBL:   AL009126
- PIR:   S60089
- RefSeq:   NP_391605.1
- ProteinModelPortal:   P45860
- SMR:   P45860
- EnsemblBacteria:   EBBACT00000001281
- GeneID:   938453
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37240
- NMPDR:   fig|224308.1.peg.3731
- GenoList:   BSU37240
- GeneTree:   EBGT00070000032016
- HOGENOM:   HBG694312
- OMA:   IHIEYYM
- PhylomeDB:   P45860
- ProtClustDB:   CLSK888054
- BioCyc:   BSUB:BSU37240-MONOMER
- HAMAP:   MF_00190
- InterPro:   IPR022924
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: 2.7.8.-

Molecular weight: Translated: 58248; Mature: 58248

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 242-242 ACT_SITE 244-244 ACT_SITE 249-249 ACT_SITE 418-418 ACT_SITE 420-420 ACT_SITE 425-425

Signals:

None

Transmembrane regions:

HASH(0x1324fd64)-; HASH(0x13106614)-; HASH(0x11fea260)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRRLEFFFLYMMLIGAYVIWFFPVSRLEFYGGLLCYISIILFSIYSLILENRTSQHTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LWIHILVFFPIVGYVFYLFSGQLYVKGKLFKTKRMYNREKLRKLFDKEETPEVTGLKDNQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHCHHHHHHHHCCCCCCCCCCCCCCC
ERFFTYSIRAAHMNINTKSNIKVLKNGEETFPDIFKAMRKAESYIHIEYYMFKSDMLGRG
CEEEEEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCC
MMDIMMEKARQGVEVRFLYDAAGSMKLARRDIMRMKQAGVDIVPFSPLKYGFFNQKLNFR
HHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC
NHRKIVIIDGKTGFVGGLNVGKEYISRDPYIGFWRDTHLRLEGEIVQTLHAIFMLDWEYV
CCCEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEECCEEEECHHHHHHHHHHHHEEHHHH
SNEVLIDQEEYNTPVPVEGGGIYQIVATGPDMKESMSDLYYEMISSAQKSIWIATPYFVP
CCCEEEEHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCC
NESIRTALKAAATKGVEVRVMVPEKNDSFLTQYASRSYFPELLLEGIEVYSYQKGFMHQK
CHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCE
VMIIDGDLASVGTANMDMRSFQLNFEVNVFFTDAEAIRTLEAHFEEDMQESEKLSPVGFY
EEEEECCCCCCCCCCCCEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCHH
KRGVADRTKESFARLFSGVL
HHCCHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MLKRRLEFFFLYMMLIGAYVIWFFPVSRLEFYGGLLCYISIILFSIYSLILENRTSQHTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LWIHILVFFPIVGYVFYLFSGQLYVKGKLFKTKRMYNREKLRKLFDKEETPEVTGLKDNQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHCHHHHHHHHCCCCCCCCCCCCCCC
ERFFTYSIRAAHMNINTKSNIKVLKNGEETFPDIFKAMRKAESYIHIEYYMFKSDMLGRG
CEEEEEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEEEEECCCCCCC
MMDIMMEKARQGVEVRFLYDAAGSMKLARRDIMRMKQAGVDIVPFSPLKYGFFNQKLNFR
HHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC
NHRKIVIIDGKTGFVGGLNVGKEYISRDPYIGFWRDTHLRLEGEIVQTLHAIFMLDWEYV
CCCEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEECCEEEECHHHHHHHHHHHHEEHHHH
SNEVLIDQEEYNTPVPVEGGGIYQIVATGPDMKESMSDLYYEMISSAQKSIWIATPYFVP
CCCEEEEHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCC
NESIRTALKAAATKGVEVRVMVPEKNDSFLTQYASRSYFPELLLEGIEVYSYQKGFMHQK
CHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCE
VMIIDGDLASVGTANMDMRSFQLNFEVNVFFTDAEAIRTLEAHFEEDMQESEKLSPVGFY
EEEEECCCCCCCCCCCCEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCHH
KRGVADRTKESFARLFSGVL
HHCCHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377