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The map label for this gene is glnK

Identifier: 16080705

GI number: 16080705

Start: 3758016

End: 3758366

Strand: Direct

Name: glnK

Synonym: BSU36520

Alternate gene names: 16080705

Gene position: 3758016-3758366 (Clockwise)

Preceding gene: 16080704

Following gene: 16080706

Centisome position: 89.15

GC content: 43.87

Gene sequence:

>351_bases
ATGAGCGGTCAAATGTTCAAGGTAGAAATTGTAACGCGTCCGGCAAATTTTGAAAAGCTGAAGCAGGAACTCGGAAAAAT
CGGAGTGACCTCTCTGACTTTCTCCAATGTACACGGCTGCGGCCTTCAAAAAGCACATACGGAGCTCTATCGAGGGGTAA
AAATAGAAAGCAATGTATACGAGCGTTTAAAAATAGAAATTGTGGTCAGCAAGGTTCCTGTTGATCAAGTGACAGAGACC
GCTAAAAGGGTGCTGAAAACGGGATCACCAGGTGACGGTAAAATATTTGTCTATGAAATCAGCAATACGATCAACATCCG
CACAGGCGAAGAAGGACCTGAAGCACTTTAA

Upstream 100 bases:

>100_bases
TCCTTCCCCTTCGCGCAACTGAAGAAGAAGAGTCACTTGGGCTTGACTTAACGATGCACGGGGAAAAAGCATATCAAGAT
TCTATGTGAGGAGTGACGCT

Downstream 100 bases:

>100_bases
TATCGGTACGAGATTCGGACACTCCGGATCTCTTTTTTTGTGCACAGAATCCCCCCAGAAACCGCGATTCCTCTTCGAAT
TCTCTTCAAGCGCCGTTATT

Product: nitrogen-regulated PII-like regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 116; Mature: 115

Protein sequence:

>116_residues
MSGQMFKVEIVTRPANFEKLKQELGKIGVTSLTFSNVHGCGLQKAHTELYRGVKIESNVYERLKIEIVVSKVPVDQVTET
AKRVLKTGSPGDGKIFVYEISNTINIRTGEEGPEAL

Sequences:

>Translated_116_residues
MSGQMFKVEIVTRPANFEKLKQELGKIGVTSLTFSNVHGCGLQKAHTELYRGVKIESNVYERLKIEIVVSKVPVDQVTET
AKRVLKTGSPGDGKIFVYEISNTINIRTGEEGPEAL
>Mature_115_residues
SGQMFKVEIVTRPANFEKLKQELGKIGVTSLTFSNVHGCGLQKAHTELYRGVKIESNVYERLKIEIVVSKVPVDQVTETA
KRVLKTGSPGDGKIFVYEISNTINIRTGEEGPEAL

Specific function: Required for full induction of the nrgAB operon under conditions of ammonium limitation

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=41.0714285714286, Blast_Score=97, Evalue=4e-22,
Organism=Escherichia coli, GI1786655, Length=109, Percent_Identity=38.5321100917431, Blast_Score=87, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRGB_BACSU (Q07428)

Other databases:

- EMBL:   L03216
- EMBL:   Z82987
- EMBL:   AL009126
- PIR:   B36865
- RefSeq:   NP_391533.1
- ProteinModelPortal:   Q07428
- SMR:   Q07428
- EnsemblBacteria:   EBBACT00000000055
- GeneID:   936937
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36520
- NMPDR:   fig|224308.1.peg.3659
- GenoList:   BSU36520
- GeneTree:   EBGT00050000002165
- HOGENOM:   HBG748089
- OMA:   DEQADDI
- ProtClustDB:   CLSK888028
- BioCyc:   BSUB:BSU36520-MONOMER
- GO:   GO:0006350
- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- Gene3D:   G3DSA:3.30.70.120
- PRINTS:   PR00340
- SMART:   SM00938

Pfam domain/function: PF00543 P-II; SSF54913 N-reg_PII-like_a/b

EC number: NA

Molecular weight: Translated: 12822; Mature: 12691

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00638 PII_GLNB_CTER; PS51343 PII_GLNB_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQMFKVEIVTRPANFEKLKQELGKIGVTSLTFSNVHGCGLQKAHTELYRGVKIESNVY
CCCCEEEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCHH
ERLKIEIVVSKVPVDQVTETAKRVLKTGSPGDGKIFVYEISNTINIRTGEEGPEAL
EEEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEECCCCCCCC
>Mature Secondary Structure 
SGQMFKVEIVTRPANFEKLKQELGKIGVTSLTFSNVHGCGLQKAHTELYRGVKIESNVY
CCCEEEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCHH
ERLKIEIVVSKVPVDQVTETAKRVLKTGSPGDGKIFVYEISNTINIRTGEEGPEAL
EEEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377