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The map label for this gene is amtB

Identifier: 16080704

GI number: 16080704

Start: 3756790

End: 3758004

Strand: Direct

Name: amtB

Synonym: BSU36510

Alternate gene names: 16080704

Gene position: 3756790-3758004 (Clockwise)

Preceding gene: 16080698

Following gene: 16080705

Centisome position: 89.12

GC content: 50.45

Gene sequence:

>1215_bases
ATGCAAATGGGCGATACAGTTTTTATGTTCTTTTGCGCTTTACTCGTGTGGCTGATGACCCCGGGATTAGCGTTATTTTA
TGGAGGAATGGTAAAGAGCAAAAATGTGCTGAGCACTGCCATGCACAGTTTCTCTTCCATTGCCATCGTTTCCATCGTTT
GGGTGCTGTTCGGATATACACTTGCCTTCGCACCAGGCAATTCAATCATCGGCGGGCTGGAGTGGGCAGGCCTCAAAGGG
GTCGGATTTGATCCGGGAGATTACAGCGATACCATCCCCCACTCGTTATTTATGATGTTCCAAATGACGTTCGCCGTTCT
GACTACAGCGATTATTTCCGGGGCTTTCGCAGAGCGGATGCGATTCGGCGCTTTTCTTTTATTCTCGGTTTTATGGGCCT
CTTTGGTTTACACACCCGTAGCGCACTGGGTATGGGGCGGCGGCTGGATCGGCCAGCTTGGAGCGCTCGATTTCGCTGGC
GGCAATGTTGTTCATATTTCCTCCGGGGTGGCAGGACTTGTTCTTGCCATTGTGCTCGGCAAACGGAAAGACGGTACAGC
GTCTTCTCCGCATAACCTCATTTACACCTTCTTAGGAGGAGCTTTGATTTGGTTCGGCTGGTTCGGCTTTAACGTCGGCA
GCGCATTGACCTTAGATGGTGTGGCCATGTACGCGTTCATCAACACAAACACCGCGGCTGCAGCCGGGATCGCCGGCTGG
ATCTTAGTAGAATGGATCATTAACAAAAAACCGACAATGCTCGGAGCGGTATCTGGGGCAATCGCCGGGCTTGTCGCCAT
TACGCCGGCTGCCGGATTTGTCACACCGTTCGCTTCCATTATTATCGGCATCATCGGCGGAGCTGTTTGTTTCTGGGGAG
TATTCTCGCTTAAAAAGAAATTCGGATACGACGACGCGCTTGACGCCTTTGGCCTGCACGGGATCGGCGGCACATGGGGC
GGAATCGCAACAGGATTATTCGCAACAACCTCTGTTAACTCAGCGGGCGCAGATGGGTTATTTTACGGTGATGCAAGCTT
AATCTGGAAACAAATCGTCGCCATCGCCGCCACTTATGTTTTTGTATTTATTGTCACTTTCGTTATTATTAAAATTGTAA
GCCTCTTCCTTCCCCTTCGCGCAACTGAAGAAGAAGAGTCACTTGGGCTTGACTTAACGATGCACGGGGAAAAAGCATAT
CAAGATTCTATGTGA

Upstream 100 bases:

>100_bases
TCAGGAAATCTTACATGAAAATGTTTTATCATTCTTTTTTCTCTATAATGAAGAAATATAATAATTGCTTTTTATTCTGA
AAGATACGGAGGAATGAGAC

Downstream 100 bases:

>100_bases
GGAGTGACGCTATGAGCGGTCAAATGTTCAAGGTAGAAATTGTAACGCGTCCGGCAAATTTTGAAAAGCTGAAGCAGGAA
CTCGGAAAAATCGGAGTGAC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MQMGDTVFMFFCALLVWLMTPGLALFYGGMVKSKNVLSTAMHSFSSIAIVSIVWVLFGYTLAFAPGNSIIGGLEWAGLKG
VGFDPGDYSDTIPHSLFMMFQMTFAVLTTAIISGAFAERMRFGAFLLFSVLWASLVYTPVAHWVWGGGWIGQLGALDFAG
GNVVHISSGVAGLVLAIVLGKRKDGTASSPHNLIYTFLGGALIWFGWFGFNVGSALTLDGVAMYAFINTNTAAAAGIAGW
ILVEWIINKKPTMLGAVSGAIAGLVAITPAAGFVTPFASIIIGIIGGAVCFWGVFSLKKKFGYDDALDAFGLHGIGGTWG
GIATGLFATTSVNSAGADGLFYGDASLIWKQIVAIAATYVFVFIVTFVIIKIVSLFLPLRATEEEESLGLDLTMHGEKAY
QDSM

Sequences:

>Translated_404_residues
MQMGDTVFMFFCALLVWLMTPGLALFYGGMVKSKNVLSTAMHSFSSIAIVSIVWVLFGYTLAFAPGNSIIGGLEWAGLKG
VGFDPGDYSDTIPHSLFMMFQMTFAVLTTAIISGAFAERMRFGAFLLFSVLWASLVYTPVAHWVWGGGWIGQLGALDFAG
GNVVHISSGVAGLVLAIVLGKRKDGTASSPHNLIYTFLGGALIWFGWFGFNVGSALTLDGVAMYAFINTNTAAAAGIAGW
ILVEWIINKKPTMLGAVSGAIAGLVAITPAAGFVTPFASIIIGIIGGAVCFWGVFSLKKKFGYDDALDAFGLHGIGGTWG
GIATGLFATTSVNSAGADGLFYGDASLIWKQIVAIAATYVFVFIVTFVIIKIVSLFLPLRATEEEESLGLDLTMHGEKAY
QDSM
>Mature_404_residues
MQMGDTVFMFFCALLVWLMTPGLALFYGGMVKSKNVLSTAMHSFSSIAIVSIVWVLFGYTLAFAPGNSIIGGLEWAGLKG
VGFDPGDYSDTIPHSLFMMFQMTFAVLTTAIISGAFAERMRFGAFLLFSVLWASLVYTPVAHWVWGGGWIGQLGALDFAG
GNVVHISSGVAGLVLAIVLGKRKDGTASSPHNLIYTFLGGALIWFGWFGFNVGSALTLDGVAMYAFINTNTAAAAGIAGW
ILVEWIINKKPTMLGAVSGAIAGLVAITPAAGFVTPFASIIIGIIGGAVCFWGVFSLKKKFGYDDALDAFGLHGIGGTWG
GIATGLFATTSVNSAGADGLFYGDASLIWKQIVAIAATYVFVFIVTFVIIKIVSLFLPLRATEEEESLGLDLTMHGEKAY
QDSM

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family

Homologues:

Organism=Escherichia coli, GI1786656, Length=401, Percent_Identity=46.3840399002494, Blast_Score=333, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI133931100, Length=428, Percent_Identity=28.0373831775701, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17550182, Length=442, Percent_Identity=29.4117647058824, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=425, Percent_Identity=27.2941176470588, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17550180, Length=434, Percent_Identity=27.8801843317972, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6324187, Length=408, Percent_Identity=38.4803921568627, Blast_Score=246, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6325396, Length=411, Percent_Identity=37.956204379562, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=413, Percent_Identity=36.319612590799, Blast_Score=219, Evalue=8e-58,
Organism=Drosophila melanogaster, GI161078314, Length=421, Percent_Identity=29.2161520190024, Blast_Score=141, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRGA_BACSU (Q07429)

Other databases:

- EMBL:   L03216
- EMBL:   Z82987
- EMBL:   AL009126
- PIR:   A36865
- RefSeq:   NP_391532.1
- ProteinModelPortal:   Q07429
- SMR:   Q07429
- EnsemblBacteria:   EBBACT00000001577
- GeneID:   936933
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36510
- NMPDR:   fig|224308.1.peg.3658
- GenoList:   BSU36510
- GeneTree:   EBGT00050000002338
- HOGENOM:   HBG677626
- OMA:   SRVFLHG
- PhylomeDB:   Q07429
- ProtClustDB:   CLSK888027
- BioCyc:   BSUB:BSU36510-MONOMER
- InterPro:   IPR001905
- InterPro:   IPR018047
- PANTHER:   PTHR11730
- TIGRFAMs:   TIGR00836

Pfam domain/function: PF00909 Ammonium_transp; SSF111352 RH_like_transpt

EC number: NA

Molecular weight: Translated: 42733; Mature: 42733

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12e2a064)-; HASH(0x12b72294)-; HASH(0x1292e9b4)-; HASH(0x11782a84)-; HASH(0x12c15dfc)-; HASH(0x12cb4e14)-; HASH(0x12e00ef8)-; HASH(0x40c07948)-; HASH(0x12a48660)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMGDTVFMFFCALLVWLMTPGLALFYGGMVKSKNVLSTAMHSFSSIAIVSIVWVLFGYT
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFAPGNSIIGGLEWAGLKGVGFDPGDYSDTIPHSLFMMFQMTFAVLTTAIISGAFAERM
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFGAFLLFSVLWASLVYTPVAHWVWGGGWIGQLGALDFAGGNVVHISSGVAGLVLAIVLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEECCCCEEEECCCHHHHHHHHHHC
KRKDGTASSPHNLIYTFLGGALIWFGWFGFNVGSALTLDGVAMYAFINTNTAAAAGIAGW
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEEECCCHHHHHHHHH
ILVEWIINKKPTMLGAVSGAIAGLVAITPAAGFVTPFASIIIGIIGGAVCFWGVFSLKKK
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGYDDALDAFGLHGIGGTWGGIATGLFATTSVNSAGADGLFYGDASLIWKQIVAIAATYV
CCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
FVFIVTFVIIKIVSLFLPLRATEEEESLGLDLTMHGEKAYQDSM
HHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEEECCCHHHCCCH
>Mature Secondary Structure
MQMGDTVFMFFCALLVWLMTPGLALFYGGMVKSKNVLSTAMHSFSSIAIVSIVWVLFGYT
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFAPGNSIIGGLEWAGLKGVGFDPGDYSDTIPHSLFMMFQMTFAVLTTAIISGAFAERM
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFGAFLLFSVLWASLVYTPVAHWVWGGGWIGQLGALDFAGGNVVHISSGVAGLVLAIVLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEECCCCEEEECCCHHHHHHHHHHC
KRKDGTASSPHNLIYTFLGGALIWFGWFGFNVGSALTLDGVAMYAFINTNTAAAAGIAGW
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEEECCCHHHHHHHHH
ILVEWIINKKPTMLGAVSGAIAGLVAITPAAGFVTPFASIIIGIIGGAVCFWGVFSLKKK
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGYDDALDAFGLHGIGGTWGGIATGLFATTSVNSAGADGLFYGDASLIWKQIVAIAATYV
CCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
FVFIVTFVIIKIVSLFLPLRATEEEESLGLDLTMHGEKAYQDSM
HHHHHHHHHHHHHHHHHCCCCCCCHHHCCCEEEECCCHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377