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The map label for this gene is ywrK

Identifier: 16080656

GI number: 16080656

Start: 3712617

End: 3713945

Strand: Direct

Name: ywrK

Synonym: BSU36030

Alternate gene names: 16080656

Gene position: 3712617-3713945 (Clockwise)

Preceding gene: 16080655

Following gene: 16080660

Centisome position: 88.07

GC content: 37.77

Gene sequence:

>1329_bases
TTGCTCGCTTTTTTCGTAACAATGATTTTTATTTTCTGGAGACCAAAAGGACTAAATGAAGCTATCCCCGCCACATTTGG
CGCTTTAATGGTTTTATTGTGCGGAAGTGTGTCATTGGCAGACTTAGGGGAAATCGGAACAAAAGTCACGGGTGCATCGG
TTACAATCCTTGCCACTATGATTATGGCCATTGCACTAGAAAGCTTTGGTTTCTTTTATTGGGTGGCTGCAAAACTGCTC
CAACAATCCAAGGGTTCAGGCATCAAGCTTTTTTGGCTAACCAATTTGCTCTGCTTTCTCATGACCATTTTCCTTAATAA
TGATGGGAGCATTTTGATCACAACTCCAATCCTTTTGCTTGTACTAAAATATTTAGGTTTAAAAAAACATCAAAAAGCCC
CATATTTATTAAGCGGTGTTTTAATAGCCACAGCCTCTAGCGCACCGATTGGCGTAAGTAATATTGTTAATTTAATTTCT
CTCAAAATCATTGGAATGGATCTATATCTTCACACCGCCATGATGTTTGTCCCTAGTATGATGGGACTCATTTTTATGAC
TTGTCTACTGTTTATGTTCTTTTATAAACGTCTTCCCAAAAGCCTGCCAGATATACCGGGCCATTTTCAGTCTCTGCGGC
ATAGAAGGTACCATCCTCTTCATTCGCCATCAGCTCCTTTACCAGAACGAAATCAAACAAAAATCATGTTGTTTGTGCTT
GCATTTGTTTTTCTCGTCAGAATGAGTCTATTTGCAGCATCATATACCGGTATTTCTGTTCCTCTAGTCGCTGTCATCGG
ATCTTTTATCCTGTTGAGCTGGAGATGGATTTATTTCAAAACCTCCCCAAGAGACTTGTTATATAAATCGCCTTGGCACA
TTTTTATATTCGCTTTTACTATGTATGTCTTAATTTATGGTCTGCATAATATTGGATTCACAGAGTTACTTGTGAGTTAT
TTTGAACCTGTGGTTTCTGGAAGCCTCGCCCATGCAACTTTCGCATCAGGTATCTCTACTTCAGTTTTTTCTAATCTCTT
TAACAACCATCCAGCGTTAATGATCAGTACTTTTACCTTAACTGAAATGACCCTGAATCCTTCAACAACAAAAATCATCT
ACTTGGCAAATATTATCGGCAGTGATATCGGTTCATTATTATTACCTATGGGAACCTTAGCAACGCTTATTTGGATGCAT
ATATTAAAACAGCATGATGAAAGTATTTCATGGGGCGAATATATAAAAACGACCATTATCATCATTCCGCTGACTGTTCT
TTTTACGTTAACATGTTTATATTTTTGGATCAGTTGGTTATTTCTTTAG

Upstream 100 bases:

>100_bases
TAGTTGGCAAGGACATCAGTAAGCGCAGGACATTAGTTTGATTTTAAAAATGAAAGATAGAGAGGTGAAGTAAATTATTG
AGTTCACCGGCATATTCACA

Downstream 100 bases:

>100_bases
CACAGTATGAAACGAAAAACTGCCTTCTGCTTTTCAAGAGGGCAGTTTTGTATACACTAGGGAAGATGCATTGTCTTTAG
ATGATTTAACTTCCAAATGA

Product: Na+/H+ antiporter

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MLAFFVTMIFIFWRPKGLNEAIPATFGALMVLLCGSVSLADLGEIGTKVTGASVTILATMIMAIALESFGFFYWVAAKLL
QQSKGSGIKLFWLTNLLCFLMTIFLNNDGSILITTPILLLVLKYLGLKKHQKAPYLLSGVLIATASSAPIGVSNIVNLIS
LKIIGMDLYLHTAMMFVPSMMGLIFMTCLLFMFFYKRLPKSLPDIPGHFQSLRHRRYHPLHSPSAPLPERNQTKIMLFVL
AFVFLVRMSLFAASYTGISVPLVAVIGSFILLSWRWIYFKTSPRDLLYKSPWHIFIFAFTMYVLIYGLHNIGFTELLVSY
FEPVVSGSLAHATFASGISTSVFSNLFNNHPALMISTFTLTEMTLNPSTTKIIYLANIIGSDIGSLLLPMGTLATLIWMH
ILKQHDESISWGEYIKTTIIIIPLTVLFTLTCLYFWISWLFL

Sequences:

>Translated_442_residues
MLAFFVTMIFIFWRPKGLNEAIPATFGALMVLLCGSVSLADLGEIGTKVTGASVTILATMIMAIALESFGFFYWVAAKLL
QQSKGSGIKLFWLTNLLCFLMTIFLNNDGSILITTPILLLVLKYLGLKKHQKAPYLLSGVLIATASSAPIGVSNIVNLIS
LKIIGMDLYLHTAMMFVPSMMGLIFMTCLLFMFFYKRLPKSLPDIPGHFQSLRHRRYHPLHSPSAPLPERNQTKIMLFVL
AFVFLVRMSLFAASYTGISVPLVAVIGSFILLSWRWIYFKTSPRDLLYKSPWHIFIFAFTMYVLIYGLHNIGFTELLVSY
FEPVVSGSLAHATFASGISTSVFSNLFNNHPALMISTFTLTEMTLNPSTTKIIYLANIIGSDIGSLLLPMGTLATLIWMH
ILKQHDESISWGEYIKTTIIIIPLTVLFTLTCLYFWISWLFL
>Mature_442_residues
MLAFFVTMIFIFWRPKGLNEAIPATFGALMVLLCGSVSLADLGEIGTKVTGASVTILATMIMAIALESFGFFYWVAAKLL
QQSKGSGIKLFWLTNLLCFLMTIFLNNDGSILITTPILLLVLKYLGLKKHQKAPYLLSGVLIATASSAPIGVSNIVNLIS
LKIIGMDLYLHTAMMFVPSMMGLIFMTCLLFMFFYKRLPKSLPDIPGHFQSLRHRRYHPLHSPSAPLPERNQTKIMLFVL
AFVFLVRMSLFAASYTGISVPLVAVIGSFILLSWRWIYFKTSPRDLLYKSPWHIFIFAFTMYVLIYGLHNIGFTELLVSY
FEPVVSGSLAHATFASGISTSVFSNLFNNHPALMISTFTLTEMTLNPSTTKIIYLANIIGSDIGSLLLPMGTLATLIWMH
ILKQHDESISWGEYIKTTIIIIPLTVLFTLTCLYFWISWLFL

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family

Homologues:

Organism=Escherichia coli, GI87082277, Length=435, Percent_Identity=29.8850574712644, Blast_Score=192, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YWRK_BACSU (O05224)

Other databases:

- EMBL:   Z93767
- EMBL:   AL009126
- PIR:   B70069
- RefSeq:   NP_391484.1
- ProteinModelPortal:   O05224
- EnsemblBacteria:   EBBACT00000001136
- GeneID:   936864
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36030
- NMPDR:   fig|224308.1.peg.3610
- GenoList:   BSU36030
- GeneTree:   EBGT00050000001367
- HOGENOM:   HBG338381
- OMA:   IEIWITI
- ProtClustDB:   CLSK887997
- BioCyc:   BSUB:BSU36030-MONOMER
- InterPro:   IPR000802
- PRINTS:   PR00758

Pfam domain/function: PF02040 ArsB

EC number: NA

Molecular weight: Translated: 49546; Mature: 49546

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12c84650)-; HASH(0x11241928)-; HASH(0x1272761c)-; HASH(0x12466748)-; HASH(0x12aaffa4)-; HASH(0x40c04d68)-; HASH(0x12d712c8)-; HASH(0x12d79590)-; HASH(0x12660c3c)-; HASH(0x12d899dc)-; HASH(0x124abe78)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHH
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377