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The map label for this gene is alsR

Identifier: 16080655

GI number: 16080655

Start: 3711498

End: 3712406

Strand: Direct

Name: alsR

Synonym: BSU36020

Alternate gene names: 16080655

Gene position: 3711498-3712406 (Clockwise)

Preceding gene: 16080651

Following gene: 16080656

Centisome position: 88.04

GC content: 43.45

Gene sequence:

>909_bases
ATGGAGCTTCGCCATCTTCAATACTTTATCGCAGTAGCCGAAGAGCTTCATTTCGGAAAGGCTGCCCGGCGGCTGAACAT
GACGCAGCCTCCTCTCAGCCAGCAGATCAAACAGCTGGAGGAAGAAGTCGGAGTTACGCTTCTGAAAAGGACAAAACGAT
TTGTCGAGCTTACCGCAGCAGGAGAAATCTTTTTAAATCATTGCCGCATGGCATTGATGCAAATCGGACAAGGAATTGAA
CTGGCACAGCGGACGGCCCGCGGCGAGCAAGGCCTTCTCGTAATCGGTTTTGTCGGATCAGCTACATATGAATTTCTGCC
GCCGATTGTCCGGGAATATCGTAAAAAATTCCCATCTGTGAAAATAGAACTGCGTGAAATATCCTCGTCTAGGCAGCAGG
AGGAGCTACTAAAGGGCAACATTGATATTGGTATACTTCATCCTCCCTTACAGCATACAGCTTTACATATCGAAACCGCC
CAAAGCAGCCCTTGTGTTTTAGCTTTGCCTAAGCAACACCCATTGACTTCTAAGGAATCAATTACGATTGAGGATTTAAG
AGATGAACCAATTATTACTGTTGCTAAAGAAGCATGGCCTACTCTATACATGGATTTTATTCAGTTCTGTGAACAAGCGG
GCTTCAGACCCAACATTGTCCAGGAAGCCACAGAATATCAAATGGTGATTGGTTTAGTCAGTGCCGGAATTGGTATGACA
TTTGTTCCTTCCTCGGCCAAAAAATTATTTAATTTGGATGTTACTTATCGAAAAATGGACCAAATACAGCTTAATGCAGA
ATGGGTTATCGCTTACCGCAAAGACAATCATAATCCGCTTATAAAGCATTTTATTCATATTTCAAATTGTCAGCAGACAA
GAACTAAAGAGAGTGATGCAGGTACATGA

Upstream 100 bases:

>100_bases
TTTCACTATACACTCTTTGGAAATTGACCTCCAATATCGATTCGCCTTATGATTAATATGTAATACGAATTATTAACCTA
TGGAAAGGAATGCATATTGA

Downstream 100 bases:

>100_bases
ACAATACTTGCAATCTCTTTTTTCTTTTGGGATTCTTATGGAATTTTTATTAATTTAATATAATCGCTTAGTACAACTTT
ATATCAGTATACGTATACAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Als operon regulatory protein

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MELRHLQYFIAVAEELHFGKAARRLNMTQPPLSQQIKQLEEEVGVTLLKRTKRFVELTAAGEIFLNHCRMALMQIGQGIE
LAQRTARGEQGLLVIGFVGSATYEFLPPIVREYRKKFPSVKIELREISSSRQQEELLKGNIDIGILHPPLQHTALHIETA
QSSPCVLALPKQHPLTSKESITIEDLRDEPIITVAKEAWPTLYMDFIQFCEQAGFRPNIVQEATEYQMVIGLVSAGIGMT
FVPSSAKKLFNLDVTYRKMDQIQLNAEWVIAYRKDNHNPLIKHFIHISNCQQTRTKESDAGT

Sequences:

>Translated_302_residues
MELRHLQYFIAVAEELHFGKAARRLNMTQPPLSQQIKQLEEEVGVTLLKRTKRFVELTAAGEIFLNHCRMALMQIGQGIE
LAQRTARGEQGLLVIGFVGSATYEFLPPIVREYRKKFPSVKIELREISSSRQQEELLKGNIDIGILHPPLQHTALHIETA
QSSPCVLALPKQHPLTSKESITIEDLRDEPIITVAKEAWPTLYMDFIQFCEQAGFRPNIVQEATEYQMVIGLVSAGIGMT
FVPSSAKKLFNLDVTYRKMDQIQLNAEWVIAYRKDNHNPLIKHFIHISNCQQTRTKESDAGT
>Mature_302_residues
MELRHLQYFIAVAEELHFGKAARRLNMTQPPLSQQIKQLEEEVGVTLLKRTKRFVELTAAGEIFLNHCRMALMQIGQGIE
LAQRTARGEQGLLVIGFVGSATYEFLPPIVREYRKKFPSVKIELREISSSRQQEELLKGNIDIGILHPPLQHTALHIETA
QSSPCVLALPKQHPLTSKESITIEDLRDEPIITVAKEAWPTLYMDFIQFCEQAGFRPNIVQEATEYQMVIGLVSAGIGMT
FVPSSAKKLFNLDVTYRKMDQIQLNAEWVIAYRKDNHNPLIKHFIHISNCQQTRTKESDAGT

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787879, Length=293, Percent_Identity=35.8361774744027, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI2367136, Length=285, Percent_Identity=36.140350877193, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1788887, Length=287, Percent_Identity=34.1463414634146, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI157672245, Length=242, Percent_Identity=31.8181818181818, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=30.8943089430894, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI145693105, Length=245, Percent_Identity=26.530612244898, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790208, Length=296, Percent_Identity=26.6891891891892, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787530, Length=254, Percent_Identity=26.3779527559055, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081904, Length=220, Percent_Identity=27.7272727272727, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=24.6963562753036, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788297, Length=153, Percent_Identity=33.9869281045752, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790262, Length=248, Percent_Identity=26.2096774193548, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787806, Length=248, Percent_Identity=24.1935483870968, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=30.8724832214765, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789173, Length=185, Percent_Identity=26.4864864864865, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786401, Length=152, Percent_Identity=28.2894736842105, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788296, Length=280, Percent_Identity=23.2142857142857, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=245, Percent_Identity=22.8571428571429, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787589, Length=123, Percent_Identity=35.7723577235772, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789506, Length=188, Percent_Identity=25.531914893617, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789639, Length=171, Percent_Identity=26.9005847953216, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): ALSR_BACSU (Q04778)

Other databases:

- EMBL:   L04470
- EMBL:   Z93767
- EMBL:   AL009126
- PIR:   A47126
- RefSeq:   NP_391483.1
- ProteinModelPortal:   Q04778
- SMR:   Q04778
- EnsemblBacteria:   EBBACT00000000620
- GeneID:   936860
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU36020
- NMPDR:   fig|224308.1.peg.3609
- GenoList:   BSU36020
- GeneTree:   EBGT00070000031936
- HOGENOM:   HBG685425
- OMA:   KEAFRIS
- PhylomeDB:   Q04778
- ProtClustDB:   CLSK2460962
- BioCyc:   BSUB:BSU36020-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34333; Mature: 34333

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLQYFIAVAEELHFGKAARRLNMTQPPLSQQIKQLEEEVGVTLLKRTKRFVELTAA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEIFLNHCRMALMQIGQGIELAQRTARGEQGLLVIGFVGSATYEFLPPIVREYRKKFPSV
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCE
KIELREISSSRQQEELLKGNIDIGILHPPLQHTALHIETAQSSPCVLALPKQHPLTSKES
EEEHHHHHCHHHHHHHHHCCCCEEEECCCHHHHEEEEEECCCCCEEEECCCCCCCCCCCC
ITIEDLRDEPIITVAKEAWPTLYMDFIQFCEQAGFRPNIVQEATEYQMVIGLVSAGIGMT
EEHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEE
FVPSSAKKLFNLDVTYRKMDQIQLNAEWVIAYRKDNHNPLIKHFIHISNCQQTRTKESDA
ECCCHHHHHEECCCHHHHHHHEEECEEEEEEEEECCCCHHHHHHHHHHCCHHHHCCCCCC
GT
CC
>Mature Secondary Structure
MELRHLQYFIAVAEELHFGKAARRLNMTQPPLSQQIKQLEEEVGVTLLKRTKRFVELTAA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEIFLNHCRMALMQIGQGIELAQRTARGEQGLLVIGFVGSATYEFLPPIVREYRKKFPSV
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCE
KIELREISSSRQQEELLKGNIDIGILHPPLQHTALHIETAQSSPCVLALPKQHPLTSKES
EEEHHHHHCHHHHHHHHHCCCCEEEECCCHHHHEEEEEECCCCCEEEECCCCCCCCCCCC
ITIEDLRDEPIITVAKEAWPTLYMDFIQFCEQAGFRPNIVQEATEYQMVIGLVSAGIGMT
EEHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEE
FVPSSAKKLFNLDVTYRKMDQIQLNAEWVIAYRKDNHNPLIKHFIHISNCQQTRTKESDA
ECCCHHHHHEECCCHHHHHHHEEECEEEEEEEEECCCCHHHHHHHHHHCCHHHHCCCCCC
GT
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377