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The map label for this gene is ywtE

Identifier: 16080638

GI number: 16080638

Start: 3695363

End: 3696223

Strand: Direct

Name: ywtE

Synonym: BSU35850

Alternate gene names: 16080638

Gene position: 3695363-3696223 (Clockwise)

Preceding gene: 255767776

Following gene: 16080644

Centisome position: 87.66

GC content: 44.6

Gene sequence:

>861_bases
ATGAAATGTATTGCGATTGATCTAGACGGAACATTACTGAATAAAGAAAGCGTCATTTCTGCGGAAAACAGAGAGGCGAT
CAAGCGGGCCGTTGATGCCGGCATCCTCGTCACCATTTGCACGGGAAGAGCGACATTTGATGTAAAAGCGCTGCTGGATG
ACTTGGACATCCCTATCATTGCAGCTAATGGCGGTACGATCCACGACACGGGTTACCGTTTGATCAGCCGGACATTAATG
GATCAGGAAGCCGGTAAAGCGATTGCTGATTACTTACTTTCAAAAAACATTTACTTTGAGGTATATACAGATGACCATCT
GCTTTCTCCTTTTGACGGCGAAGCTAAGCTGCACGCGGAGCTTGACATTCTAAAGAGCGCAAACCCAAACGAGCAGACGG
ATGACTTGTGGCAAGGAGCCATGACTCAATTCAAACAGTTTGGCATTAAACCAATCCCTCATATCGAATCGGTTTTTGAC
GGCGGTGAAAACATTTATAAGCTTCTTTGCTTCTCCTTTGATATGGACAAGCTGAAACAAGCGAAGGAAGAACTGAAGCA
TCATAAAAAACTGGCGCAAACCTCTTCAGGAAAACATATTATCGAAATCCTGCCGGCCTCGTCAGGAAAAGGACGCGCGT
TAACAAAGCTGGCTGACATATACGGAATTGAAACACAGGATATCTATGCGATCGGCGATAGTCCAAACGATTTGTCCATG
TTTGAAGTCGCCGGACACCGTATCGCCATGGAAAATGCGATTGATGAATTAAAAGAAAAAAGCACCTTTGTCACAAAAAG
CAATGACGAAAATGGTGTGGCCTACTTTATAGACCAGCTTCTTTCGGGGCAATATGCATAA

Upstream 100 bases:

>100_bases
TCAAAGTAACGTTTAGCCTTAATTTATATGGATTATGTTTCACAGGCAAGCTGAACATACTACACGAAGCAGCTTCATTT
TTAAGGAGGTTCCCCTACAT

Downstream 100 bases:

>100_bases
AGAAAAGACTCCAGAGATTGGAGTCTTTTTCTTTTATTGCACCCGTATACTTCCTGCGTATTTATCCTTCCAATATGTGC
TCGTGTTCATATTGGTAATG

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKCIAIDLDGTLLNKESVISAENREAIKRAVDAGILVTICTGRATFDVKALLDDLDIPIIAANGGTIHDTGYRLISRTLM
DQEAGKAIADYLLSKNIYFEVYTDDHLLSPFDGEAKLHAELDILKSANPNEQTDDLWQGAMTQFKQFGIKPIPHIESVFD
GGENIYKLLCFSFDMDKLKQAKEELKHHKKLAQTSSGKHIIEILPASSGKGRALTKLADIYGIETQDIYAIGDSPNDLSM
FEVAGHRIAMENAIDELKEKSTFVTKSNDENGVAYFIDQLLSGQYA

Sequences:

>Translated_286_residues
MKCIAIDLDGTLLNKESVISAENREAIKRAVDAGILVTICTGRATFDVKALLDDLDIPIIAANGGTIHDTGYRLISRTLM
DQEAGKAIADYLLSKNIYFEVYTDDHLLSPFDGEAKLHAELDILKSANPNEQTDDLWQGAMTQFKQFGIKPIPHIESVFD
GGENIYKLLCFSFDMDKLKQAKEELKHHKKLAQTSSGKHIIEILPASSGKGRALTKLADIYGIETQDIYAIGDSPNDLSM
FEVAGHRIAMENAIDELKEKSTFVTKSNDENGVAYFIDQLLSGQYA
>Mature_286_residues
MKCIAIDLDGTLLNKESVISAENREAIKRAVDAGILVTICTGRATFDVKALLDDLDIPIIAANGGTIHDTGYRLISRTLM
DQEAGKAIADYLLSKNIYFEVYTDDHLLSPFDGEAKLHAELDILKSANPNEQTDDLWQGAMTQFKQFGIKPIPHIESVFD
GGENIYKLLCFSFDMDKLKQAKEELKHHKKLAQTSSGKHIIEILPASSGKGRALTKLADIYGIETQDIYAIGDSPNDLSM
FEVAGHRIAMENAIDELKEKSTFVTKSNDENGVAYFIDQLLSGQYA

Specific function: Unknown

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family

Homologues:

Organism=Escherichia coli, GI2367265, Length=305, Percent_Identity=29.5081967213115, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786982, Length=283, Percent_Identity=25.0883392226148, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081790, Length=288, Percent_Identity=23.2638888888889, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787043, Length=297, Percent_Identity=22.8956228956229, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YWTE_BACSU (P96741)

Other databases:

- EMBL:   Z92954
- EMBL:   AL009126
- PIR:   C70070
- RefSeq:   NP_391466.1
- HSSP:   P0A8Y5
- ProteinModelPortal:   P96741
- SMR:   P96741
- EnsemblBacteria:   EBBACT00000002127
- GeneID:   936824
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35850
- NMPDR:   fig|224308.1.peg.3592
- GenoList:   BSU35850
- GeneTree:   EBGT00070000031822
- HOGENOM:   HBG728500
- OMA:   KLPARIF
- PhylomeDB:   P96741
- ProtClustDB:   CLSK887985
- BioCyc:   BSUB:BSU35850-MONOMER
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR00099
- TIGRFAMs:   TIGR01484

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: NA

Molecular weight: Translated: 31588; Mature: 31588

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01228 COF_1; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCIAIDLDGTLLNKESVISAENREAIKRAVDAGILVTICTGRATFDVKALLDDLDIPII
CEEEEEECCCEEECCHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCEEE
AANGGTIHDTGYRLISRTLMDQEAGKAIADYLLSKNIYFEVYTDDHLLSPFDGEAKLHAE
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEHH
LDILKSANPNEQTDDLWQGAMTQFKQFGIKPIPHIESVFDGGENIYKLLCFSFDMDKLKQ
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHCCHHHHHH
AKEELKHHKKLAQTSSGKHIIEILPASSGKGRALTKLADIYGIETQDIYAIGDSPNDLSM
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHH
FEVAGHRIAMENAIDELKEKSTFVTKSNDENGVAYFIDQLLSGQYA
HHHHCCHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKCIAIDLDGTLLNKESVISAENREAIKRAVDAGILVTICTGRATFDVKALLDDLDIPII
CEEEEEECCCEEECCHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCEEE
AANGGTIHDTGYRLISRTLMDQEAGKAIADYLLSKNIYFEVYTDDHLLSPFDGEAKLHAE
ECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEHH
LDILKSANPNEQTDDLWQGAMTQFKQFGIKPIPHIESVFDGGENIYKLLCFSFDMDKLKQ
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHCCHHHHHH
AKEELKHHKKLAQTSSGKHIIEILPASSGKGRALTKLADIYGIETQDIYAIGDSPNDLSM
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHH
FEVAGHRIAMENAIDELKEKSTFVTKSNDENGVAYFIDQLLSGQYA
HHHHCCHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377