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The map label for this gene is lytC

Identifier: 16080615

GI number: 16080615

Start: 3659119

End: 3660609

Strand: Reverse

Name: lytC

Synonym: BSU35620

Alternate gene names: 16080615

Gene position: 3660609-3659119 (Counterclockwise)

Preceding gene: 16080616

Following gene: 16080613

Centisome position: 86.83

GC content: 39.17

Gene sequence:

>1491_bases
TTGCGTTCTTATATAAAAGTCCTAACAATGTGTTTTCTGGGGCTCATACTTTTTGTGCCAACAGCTTTGGCCGATAACTC
AGTGAAAAGAGTTGGGGGAAGCAATAGATACGGCACTGCTGTACAAATATCAAAGCAAATGTATTCAACAGCAAGTACAG
CTGTAATTGTTGGTGGGAGTTCCTATGCAGATGCTATTTCAGCAGCACCTCTTGCTTACCAGAAGAATGCGCCATTGCTT
TACACTAATTCTGATAAGCTTTCATATGAAACGAAAACAAGATTGAAAGAGATGCAGACTAAAAATGTAATTATTGTAGG
CGGAACACCTGCTGTTTCTTCTAACACTGCTAACCAGATTAAAAGCTTGGGGATAAGTATTAAACGAATTGCAGGAAGCA
ACCGTTATGATACGGCTGCACGGGTGGCAAAAGCGATGGGTGCGACTTCAAAAGCTGTTATTTTGAACGGCTTCTTATAT
GCAGACGCTCCGGCCGTCATCCCTTATGCAGCGAAAAACGGGTATCCAATTCTTTTTACAAATAAAACATCTATAAATAG
TGCGACTACGTCTGTGATAAAAGATAAGGGAATTTCGAGTACCGTTGTTGTAGGAGGCACTGGAAGTATCAGCAATACGG
TATACAACAAGTTACCTTCTCCTACAAGAATTAGCGGTTCAAACAGATATGAGCTTGCTGCAAATATCGTACAAAAACTT
AATTTATCAACAAGCACCGTATATGTAAGCAATGGATTCAGCTACCCTGACTCTATTGCAGGAGCTACACTGGCAGCTAA
GAAGAAGCAATCTCTTATTCTTACAAATGGTGAAAATTTATCTACAGGAGCCCGTAAAATTATTGGAAGTAAAAACATGT
CAAACTTTATGATTATCGGAAACACTCCTGCCGTAAGCACAAAGGTTGCTAATCAGCTAAAGAATCCAGTTGTAGGTGAA
ACAATCTTTATTGATCCGGGTCACGGTGATCAAGATTCAGGAGCAATCGGCAATGGACTCCTTGAGAAAGAAGTCAACCT
TGATATAGCGAAAAGAGTCAATACAAAGCTAAATGCTTCAGGTGCTCTTCCAGTACTGTCAAGATCTAATGATACTTTTT
ATTCTTTACAGGAGAGAGTAAATAAAGCAGCTTCTGCACAAGCAGATTTATTTCTCAGTATACATGCAAATGCTAATGAT
AGCTCATCACCAAATGGAAGTGAGACGTACTACGATACAACATATCAAGCTGCAAATAGCAAGAGACTGGCTGAACAAAT
TCAACCAAAGTTAGCGGCTAATCTTGGAACGAGAGACCGGGGAGTAAAAACAGCTGCTTTCTATGTTATTAAATATTCTA
AAATGCCGAGTGTTTTAGTTGAAACTGCCTTTATCACTAATGCATCAGATGCAAGTAAATTGAAGCAAGCGGTTTATAAA
GATAAAGCTGCACAAGCTATTCATGACGGCACAGTATCTTATTACAGATAA

Upstream 100 bases:

>100_bases
GAGATTCATATAGTTCAATTTTGAAATTTTACTATCCAGGTACAACTCTGACAAGTTATTGATTATTTTAGGATATAACG
CCATTTAAGGAGGAAATCAA

Downstream 100 bases:

>100_bases
TCGAAAGAGACAAATCTAATCACAGATTTGTCTCTTTTTTATATGAAATGATTAAAACAAATAGGACTTGTATATAAAAT
AGTAAAAGAGTATTAACAAC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MRSYIKVLTMCFLGLILFVPTALADNSVKRVGGSNRYGTAVQISKQMYSTASTAVIVGGSSYADAISAAPLAYQKNAPLL
YTNSDKLSYETKTRLKEMQTKNVIIVGGTPAVSSNTANQIKSLGISIKRIAGSNRYDTAARVAKAMGATSKAVILNGFLY
ADAPAVIPYAAKNGYPILFTNKTSINSATTSVIKDKGISSTVVVGGTGSISNTVYNKLPSPTRISGSNRYELAANIVQKL
NLSTSTVYVSNGFSYPDSIAGATLAAKKKQSLILTNGENLSTGARKIIGSKNMSNFMIIGNTPAVSTKVANQLKNPVVGE
TIFIDPGHGDQDSGAIGNGLLEKEVNLDIAKRVNTKLNASGALPVLSRSNDTFYSLQERVNKAASAQADLFLSIHANAND
SSSPNGSETYYDTTYQAANSKRLAEQIQPKLAANLGTRDRGVKTAAFYVIKYSKMPSVLVETAFITNASDASKLKQAVYK
DKAAQAIHDGTVSYYR

Sequences:

>Translated_496_residues
MRSYIKVLTMCFLGLILFVPTALADNSVKRVGGSNRYGTAVQISKQMYSTASTAVIVGGSSYADAISAAPLAYQKNAPLL
YTNSDKLSYETKTRLKEMQTKNVIIVGGTPAVSSNTANQIKSLGISIKRIAGSNRYDTAARVAKAMGATSKAVILNGFLY
ADAPAVIPYAAKNGYPILFTNKTSINSATTSVIKDKGISSTVVVGGTGSISNTVYNKLPSPTRISGSNRYELAANIVQKL
NLSTSTVYVSNGFSYPDSIAGATLAAKKKQSLILTNGENLSTGARKIIGSKNMSNFMIIGNTPAVSTKVANQLKNPVVGE
TIFIDPGHGDQDSGAIGNGLLEKEVNLDIAKRVNTKLNASGALPVLSRSNDTFYSLQERVNKAASAQADLFLSIHANAND
SSSPNGSETYYDTTYQAANSKRLAEQIQPKLAANLGTRDRGVKTAAFYVIKYSKMPSVLVETAFITNASDASKLKQAVYK
DKAAQAIHDGTVSYYR
>Mature_496_residues
MRSYIKVLTMCFLGLILFVPTALADNSVKRVGGSNRYGTAVQISKQMYSTASTAVIVGGSSYADAISAAPLAYQKNAPLL
YTNSDKLSYETKTRLKEMQTKNVIIVGGTPAVSSNTANQIKSLGISIKRIAGSNRYDTAARVAKAMGATSKAVILNGFLY
ADAPAVIPYAAKNGYPILFTNKTSINSATTSVIKDKGISSTVVVGGTGSISNTVYNKLPSPTRISGSNRYELAANIVQKL
NLSTSTVYVSNGFSYPDSIAGATLAAKKKQSLILTNGENLSTGARKIIGSKNMSNFMIIGNTPAVSTKVANQLKNPVVGE
TIFIDPGHGDQDSGAIGNGLLEKEVNLDIAKRVNTKLNASGALPVLSRSNDTFYSLQERVNKAASAQADLFLSIHANAND
SSSPNGSETYYDTTYQAANSKRLAEQIQPKLAANLGTRDRGVKTAAFYVIKYSKMPSVLVETAFITNASDASKLKQAVYK
DKAAQAIHDGTVSYYR

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG2247

COG function: function code M; Putative cell wall-binding domain

Gene ontology:

Cell location: Secreted, cell wall

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family

Homologues:

Organism=Escherichia coli, GI87082163, Length=225, Percent_Identity=32.8888888888889, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788776, Length=219, Percent_Identity=33.7899543378995, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CWLB_BACSU (Q02114)

Other databases:

- EMBL:   M87645
- EMBL:   M81324
- EMBL:   D10388
- EMBL:   AL009126
- PIR:   B41322
- RefSeq:   NP_391442.1
- ProteinModelPortal:   Q02114
- SMR:   Q02114
- EnsemblBacteria:   EBBACT00000002402
- GeneID:   936777
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35620
- NMPDR:   fig|224308.1.peg.3568
- GenoList:   BSU35620
- GeneTree:   EBGT00070000031903
- HOGENOM:   HBG459755
- OMA:   FYVIKYS
- PhylomeDB:   Q02114
- ProtClustDB:   CLSK887969
- BioCyc:   BSUB:BSU35620-MONOMER
- BRENDA:   3.5.1.28
- InterPro:   IPR007253
- InterPro:   IPR002508
- Gene3D:   G3DSA:3.40.630.40
- SMART:   SM00646

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2

EC number: =3.5.1.28

Molecular weight: Translated: 52626; Mature: 52626

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSYIKVLTMCFLGLILFVPTALADNSVKRVGGSNRYGTAVQISKQMYSTASTAVIVGGS
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEHHHHHCCCCCEEEECCC
SYADAISAAPLAYQKNAPLLYTNSDKLSYETKTRLKEMQTKNVIIVGGTPAVSSNTANQI
HHHHHHHCCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHH
KSLGISIKRIAGSNRYDTAARVAKAMGATSKAVILNGFLYADAPAVIPYAAKNGYPILFT
HHHCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEEECCCCEEEEEE
NKTSINSATTSVIKDKGISSTVVVGGTGSISNTVYNKLPSPTRISGSNRYELAANIVQKL
CCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCEECCCCCHHHHHHHHHHC
NLSTSTVYVSNGFSYPDSIAGATLAAKKKQSLILTNGENLSTGARKIIGSKNMSNFMIIG
CCCCEEEEEECCCCCCHHHCCCHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCCCEEEEE
NTPAVSTKVANQLKNPVVGETIFIDPGHGDQDSGAIGNGLLEKEVNLDIAKRVNTKLNAS
CCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHCCCCCCCC
GALPVLSRSNDTFYSLQERVNKAASAQADLFLSIHANANDSSSPNGSETYYDTTYQAANS
CCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEEECHHHHCCH
KRLAEQIQPKLAANLGTRDRGVKTAAFYVIKYSKMPSVLVETAFITNASDASKLKQAVYK
HHHHHHHCHHHHHCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHH
DKAAQAIHDGTVSYYR
HHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRSYIKVLTMCFLGLILFVPTALADNSVKRVGGSNRYGTAVQISKQMYSTASTAVIVGGS
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEHHHHHCCCCCEEEECCC
SYADAISAAPLAYQKNAPLLYTNSDKLSYETKTRLKEMQTKNVIIVGGTPAVSSNTANQI
HHHHHHHCCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHH
KSLGISIKRIAGSNRYDTAARVAKAMGATSKAVILNGFLYADAPAVIPYAAKNGYPILFT
HHHCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEEECCCCEEEEEE
NKTSINSATTSVIKDKGISSTVVVGGTGSISNTVYNKLPSPTRISGSNRYELAANIVQKL
CCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCEECCCCCHHHHHHHHHHC
NLSTSTVYVSNGFSYPDSIAGATLAAKKKQSLILTNGENLSTGARKIIGSKNMSNFMIIG
CCCCEEEEEECCCCCCHHHCCCHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCCCEEEEE
NTPAVSTKVANQLKNPVVGETIFIDPGHGDQDSGAIGNGLLEKEVNLDIAKRVNTKLNAS
CCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHCCCCCCCC
GALPVLSRSNDTFYSLQERVNKAASAQADLFLSIHANANDSSSPNGSETYYDTTYQAANS
CCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEEECHHHHCCH
KRLAEQIQPKLAANLGTRDRGVKTAAFYVIKYSKMPSVLVETAFITNASDASKLKQAVYK
HHHHHHHCHHHHHCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHH
DKAAQAIHDGTVSYYR
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377