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The map label for this gene is degU

Identifier: 16080602

GI number: 16080602

Start: 3644607

End: 3645296

Strand: Reverse

Name: degU

Synonym: BSU35490

Alternate gene names: 16080602

Gene position: 3645296-3644607 (Counterclockwise)

Preceding gene: 16080603

Following gene: 16080601

Centisome position: 86.47

GC content: 42.32

Gene sequence:

>690_bases
GTGACTAAAGTAAACATTGTTATTATCGACGACCATCAGTTATTTCGTGAAGGTGTTAAACGGATATTGGATTTTGAACC
TACCTTTGAAGTGGTAGCCGAAGGTGATGACGGGGACGAAGCGGCTCGTATTGTTGAGCACTATCATCCTGATGTTGTGA
TCATGGATATCAATATGCCAAACGTAAATGGTGTGGAAGCTACAAAACAGCTTGTAGAGCTGTATCCTGAATCTAAAGTA
ATTATTCTATCAATTCACGATGACGAAAATTATGTAACACATGCCCTGAAAACAGGTGCAAGAGGTTATCTGCTGAAAGA
GATGGATGCTGATACATTAATTGAAGCGGTTAAAGTAGTGGCTGAGGGCGGATCTTACCTCCATCCGAAGGTTACTCACA
ACCTCGTTAACGAATTCCGCCGCCTTGCAACAAGCGGAGTTTCTGCACACCCTCAACATGAGGTTTACCCTGAAATCCGC
AGACCATTACATATTTTAACTAGGCGGGAATGTGAAGTGCTGCAGATGCTTGCAGACGGAAAAAGCAACCGCGGTATTGG
TGAATCATTGTTTATCAGTGAGAAAACCGTTAAAAACCATGTCAGCAATATTTTACAAAAAATGAATGTAAACGACCGGA
CGCAAGCCGTTGTGGTCGCCATTAAAAATGGCTGGGTAGAAATGAGATAG

Upstream 100 bases:

>100_bases
GTTCCGTTATCTCTTTGACTATGATTTGTAAAATAGAGCCAAAAGGCATATTGACCGAATGCTAGAGTATATAGAACAAT
AATACAAGGAGGCGTGGCTT

Downstream 100 bases:

>100_bases
TATAATAGGAGACTTGCCTTTTACTAGGCAGGTCTTTTTTTAGGCTGCCGTTTCCCTTACAATAGAGTTATAAAGCAATA
AGGCAGGTATCGAAGCTATG

Product: two-component response regulator

Products: NA

Alternate protein names: Protease production enhancer protein

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTKVNIVIIDDHQLFREGVKRILDFEPTFEVVAEGDDGDEAARIVEHYHPDVVIMDINMPNVNGVEATKQLVELYPESKV
IILSIHDDENYVTHALKTGARGYLLKEMDADTLIEAVKVVAEGGSYLHPKVTHNLVNEFRRLATSGVSAHPQHEVYPEIR
RPLHILTRRECEVLQMLADGKSNRGIGESLFISEKTVKNHVSNILQKMNVNDRTQAVVVAIKNGWVEMR

Sequences:

>Translated_229_residues
MTKVNIVIIDDHQLFREGVKRILDFEPTFEVVAEGDDGDEAARIVEHYHPDVVIMDINMPNVNGVEATKQLVELYPESKV
IILSIHDDENYVTHALKTGARGYLLKEMDADTLIEAVKVVAEGGSYLHPKVTHNLVNEFRRLATSGVSAHPQHEVYPEIR
RPLHILTRRECEVLQMLADGKSNRGIGESLFISEKTVKNHVSNILQKMNVNDRTQAVVVAIKNGWVEMR
>Mature_228_residues
TKVNIVIIDDHQLFREGVKRILDFEPTFEVVAEGDDGDEAARIVEHYHPDVVIMDINMPNVNGVEATKQLVELYPESKVI
ILSIHDDENYVTHALKTGARGYLLKEMDADTLIEAVKVVAEGGSYLHPKVTHNLVNEFRRLATSGVSAHPQHEVYPEIRR
PLHILTRRECEVLQMLADGKSNRGIGESLFISEKTVKNHVSNILQKMNVNDRTQAVVVAIKNGWVEMR

Specific function: Regulating factor for the production of extracellular proteases. The N-terminal region acts as an inhibitor, whereas the C-terminal region carries enhancing activity

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788521, Length=222, Percent_Identity=35.1351351351351, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1790102, Length=223, Percent_Identity=33.1838565022422, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788222, Length=222, Percent_Identity=27.027027027027, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1787473, Length=223, Percent_Identity=34.0807174887892, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788712, Length=220, Percent_Identity=28.6363636363636, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786747, Length=210, Percent_Identity=25.7142857142857, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789937, Length=216, Percent_Identity=24.0740740740741, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEGU_BACSU (P13800)

Other databases:

- EMBL:   M21658
- EMBL:   M23558
- EMBL:   M23649
- EMBL:   U56901
- EMBL:   AL009126
- PIR:   C30191
- RefSeq:   NP_391429.1
- ProteinModelPortal:   P13800
- SMR:   P13800
- EnsemblBacteria:   EBBACT00000001456
- GeneID:   936751
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU35490
- NMPDR:   fig|224308.1.peg.3555
- GenoList:   BSU35490
- GeneTree:   EBGT00070000031976
- HOGENOM:   HBG753323
- OMA:   KVTHNLI
- PhylomeDB:   P13800
- ProtClustDB:   CLSK2518281
- BioCyc:   BSUB:BSU35490-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00038
- SMART:   SM00421
- SMART:   SM00448

Pfam domain/function: PF00196 GerE; PF00072 Response_reg; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 25867; Mature: 25736

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00622 HTH_LUXR_1; PS50043 HTH_LUXR_2; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVNIVIIDDHQLFREGVKRILDFEPTFEVVAEGDDGDEAARIVEHYHPDVVIMDINMP
CCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCC
NVNGVEATKQLVELYPESKVIILSIHDDENYVTHALKTGARGYLLKEMDADTLIEAVKVV
CCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEHHCCHHHHHHHHHHH
AEGGSYLHPKVTHNLVNEFRRLATSGVSAHPQHEVYPEIRRPLHILTRRECEVLQMLADG
HCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
KSNRGIGESLFISEKTVKNHVSNILQKMNVNDRTQAVVVAIKNGWVEMR
CCCCCCCCHHEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEECC
>Mature Secondary Structure 
TKVNIVIIDDHQLFREGVKRILDFEPTFEVVAEGDDGDEAARIVEHYHPDVVIMDINMP
CEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCC
NVNGVEATKQLVELYPESKVIILSIHDDENYVTHALKTGARGYLLKEMDADTLIEAVKVV
CCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEHHCCHHHHHHHHHHH
AEGGSYLHPKVTHNLVNEFRRLATSGVSAHPQHEVYPEIRRPLHILTRRECEVLQMLADG
HCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
KSNRGIGESLFISEKTVKNHVSNILQKMNVNDRTQAVVVAIKNGWVEMR
CCCCCCCCHHEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3136143; 3141378; 3141377; 9384377