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The map label for this gene is yvbU

Identifier: 16080452

GI number: 16080452

Start: 3487974

End: 3488852

Strand: Reverse

Name: yvbU

Synonym: BSU33990

Alternate gene names: 16080452

Gene position: 3488852-3487974 (Counterclockwise)

Preceding gene: 16080454

Following gene: 16080451

Centisome position: 82.76

GC content: 47.9

Gene sequence:

>879_bases
ATGACCATTACTCAATTAAAGGTTTTTGTGAAAATAGCTGAAACGGGAAGCTTTACAAAAGCGGGTCAGGCGCTGAACAT
GACACAGCCGGCTGTCAGCCATGCGATTTCTGCGATCGAAGCTGAGCTTGATGTAAAGCTGATCATTCGTGACCGCCGCA
ATGGCCTGATGCTGACGGATACGGGAAAACAGATCTTAGTACACATCAGAGAAGTCTTAAAAGGCATTGAAAAAGTTGAG
CAGGTAGCAGCAGCGGAAAAAGGGCTTGAACTGGGAACGATCCATATCGGCACATTCTCAACTGCCTCGGCTTATTTTAT
GCCGAAGCTGATCAGTGAATTTAAACAAAAATATCCGAAACTTGAGCTCGTGCTGCACGAGGGAACTGTAAATGAAGTCA
AGGAGTGGCTTCATACGCGGATGATAGATGTTGGGATTCTTCTGTATCCAACAGAGGAGATGGAGTACATTCATTTGAAA
AAAGACAAAATGGCTGTCGTGCTCAGGGACGATCACCCGCTTGCCAGTCATTCAGCCATTACGCTGAAAGATCTTGACCA
TGAACCGATGATCGTCTGTGACGGCGGCTATGAATCACCATTTATCGATATGTTCAGGCAGGCGGGTGCTACGCTGCACC
CAGCTTTCACGGTCTATAACATCAACACCTCGATCAGTATGATCAGAGAAGGCCTGGGCCTGGCCATTTTATCAGAAATG
TCCATGTCGGGGATGCCGCTGCCAGAGCATGTGGTGACCAGGGAATTGGACCCGCAGGTATACCGTGATGTGCAGCTTGC
TGTTCCGTCGCTAAAGGAGGCTTCTCTGGCGGCTAAGCTCTTTATTGAGATGGCTAAGGAGTTGTTTGGAGCAGAATAA

Upstream 100 bases:

>100_bases
TTGTTCATTCACTCCTTGTAGAACTTTTCCATCATATGATACGGTAGAACATAAGATAAATAAAATGAATCTTTTTTATA
AGGAGCATAAGTAAATTGTT

Downstream 100 bases:

>100_bases
TGTTCCAAACCGTTCGTTTTATATTGTTCGAATTTCATCGAATCAAATTCTTTTATTCCTGAATGCAGTTGTGTTATGGT
GTGATAGAAGTTCTTTCAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTITQLKVFVKIAETGSFTKAGQALNMTQPAVSHAISAIEAELDVKLIIRDRRNGLMLTDTGKQILVHIREVLKGIEKVE
QVAAAEKGLELGTIHIGTFSTASAYFMPKLISEFKQKYPKLELVLHEGTVNEVKEWLHTRMIDVGILLYPTEEMEYIHLK
KDKMAVVLRDDHPLASHSAITLKDLDHEPMIVCDGGYESPFIDMFRQAGATLHPAFTVYNINTSISMIREGLGLAILSEM
SMSGMPLPEHVVTRELDPQVYRDVQLAVPSLKEASLAAKLFIEMAKELFGAE

Sequences:

>Translated_292_residues
MTITQLKVFVKIAETGSFTKAGQALNMTQPAVSHAISAIEAELDVKLIIRDRRNGLMLTDTGKQILVHIREVLKGIEKVE
QVAAAEKGLELGTIHIGTFSTASAYFMPKLISEFKQKYPKLELVLHEGTVNEVKEWLHTRMIDVGILLYPTEEMEYIHLK
KDKMAVVLRDDHPLASHSAITLKDLDHEPMIVCDGGYESPFIDMFRQAGATLHPAFTVYNINTSISMIREGLGLAILSEM
SMSGMPLPEHVVTRELDPQVYRDVQLAVPSLKEASLAAKLFIEMAKELFGAE
>Mature_291_residues
TITQLKVFVKIAETGSFTKAGQALNMTQPAVSHAISAIEAELDVKLIIRDRRNGLMLTDTGKQILVHIREVLKGIEKVEQ
VAAAEKGLELGTIHIGTFSTASAYFMPKLISEFKQKYPKLELVLHEGTVNEVKEWLHTRMIDVGILLYPTEEMEYIHLKK
DKMAVVLRDDHPLASHSAITLKDLDHEPMIVCDGGYESPFIDMFRQAGATLHPAFTVYNINTSISMIREGLGLAILSEMS
MSGMPLPEHVVTRELDPQVYRDVQLAVPSLKEASLAAKLFIEMAKELFGAE

Specific function: This Protein Is A Positive Regulator Of Gene Expression For The Cysteine Regulon, A System Of 10 Or More Loci Involved In The Biosynthesis Of L-Cysteine From Inorganic Sulfate. The Inducer For Cysb Is N-Acetylserine. Cysb Inhibits Its Own Transcription.

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787530, Length=263, Percent_Identity=25.4752851711027, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=26.8292682926829, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI157672245, Length=299, Percent_Identity=28.4280936454849, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI145693105, Length=299, Percent_Identity=25.4180602006689, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788748, Length=293, Percent_Identity=25.5972696245734, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787879, Length=246, Percent_Identity=29.2682926829268, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787601, Length=175, Percent_Identity=30.8571428571429, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=26.530612244898, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788297, Length=178, Percent_Identity=32.0224719101124, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081904, Length=229, Percent_Identity=26.2008733624454, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788481, Length=243, Percent_Identity=26.3374485596708, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=24.5901639344262, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367136, Length=270, Percent_Identity=25.5555555555556, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789204, Length=248, Percent_Identity=22.9838709677419, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI87082132, Length=136, Percent_Identity=30.1470588235294, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789639, Length=126, Percent_Identity=31.7460317460317, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790208, Length=254, Percent_Identity=23.6220472440945, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1786401, Length=147, Percent_Identity=29.9319727891156, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787806, Length=128, Percent_Identity=28.125, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790262, Length=290, Percent_Identity=22.7586206896552, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YVBU_BACSU (O32255)

Other databases:

- EMBL:   AL009126
- PIR:   E70030
- RefSeq:   NP_391279.1
- ProteinModelPortal:   O32255
- SMR:   O32255
- EnsemblBacteria:   EBBACT00000000194
- GeneID:   937711
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33990
- NMPDR:   fig|224308.1.peg.3405
- GenoList:   BSU33990
- GeneTree:   EBGT00070000031793
- HOGENOM:   HBG685425
- OMA:   NTSISMI
- PhylomeDB:   O32255
- ProtClustDB:   CLSK887894
- BioCyc:   BSUB:BSU33990-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 32513; Mature: 32382

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITQLKVFVKIAETGSFTKAGQALNMTQPAVSHAISAIEAELDVKLIIRDRRNGLMLTD
CCHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEC
TGKQILVHIREVLKGIEKVEQVAAAEKGLELGTIHIGTFSTASAYFMPKLISEFKQKYPK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
LELVLHEGTVNEVKEWLHTRMIDVGILLYPTEEMEYIHLKKDKMAVVLRDDHPLASHSAI
EEEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCEE
TLKDLDHEPMIVCDGGYESPFIDMFRQAGATLHPAFTVYNINTSISMIREGLGLAILSEM
EEEECCCCCEEEECCCCCCHHHHHHHHCCCEECCEEEEEEECCHHHHHHHCCCHHHHHHH
SMSGMPLPEHVVTRELDPQVYRDVQLAVPSLKEASLAAKLFIEMAKELFGAE
CCCCCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TITQLKVFVKIAETGSFTKAGQALNMTQPAVSHAISAIEAELDVKLIIRDRRNGLMLTD
CHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEC
TGKQILVHIREVLKGIEKVEQVAAAEKGLELGTIHIGTFSTASAYFMPKLISEFKQKYPK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
LELVLHEGTVNEVKEWLHTRMIDVGILLYPTEEMEYIHLKKDKMAVVLRDDHPLASHSAI
EEEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCEE
TLKDLDHEPMIVCDGGYESPFIDMFRQAGATLHPAFTVYNINTSISMIREGLGLAILSEM
EEEECCCCCEEEECCCCCCHHHHHHHHCCCEECCEEEEEEECCHHHHHHHCCCHHHHHHH
SMSGMPLPEHVVTRELDPQVYRDVQLAVPSLKEASLAAKLFIEMAKELFGAE
CCCCCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377