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The map label for this gene is sufS

Identifier: 16080321

GI number: 16080321

Start: 3357444

End: 3358664

Strand: Reverse

Name: sufS

Synonym: BSU32690

Alternate gene names: 16080321

Gene position: 3358664-3357444 (Counterclockwise)

Preceding gene: 16080322

Following gene: 16080320

Centisome position: 79.67

GC content: 48.08

Gene sequence:

>1221_bases
ATGAATATCACAGATATTCGTGAACAGTTCCCGATCCTTCATCAGCAAGTGAACGGACATGATCTCGTTTATCTCGACAG
CGCGGCGACTTCCCAGAAGCCGCGTGCTGTCATTGAAACACTGGATAAGTATTATAACCAATACAATTCCAATGTTCACC
GGGGCGTCCATACACTTGGAACCAGAGCGACAGATGGTTATGAAGGAGCGCGTGAAAAAGTCCGCAAGTTTATTAACGCG
AAGTCAATGGCTGAGATTATTTTCACAAAAGGCACGACCACATCACTGAATATGGTGGCGTTAAGCTATGCGCGCGCCAA
CCTGAAACCTGGTGATGAAGTGGTCATCACCTACATGGAGCATCATGCGAATATCATTCCGTGGCAGCAGGCTGTAAAAG
CAACTGGCGCCACATTAAAATATATTCCGTTGCAGGAAGACGGAACGATTTCTTTAGAAGACGTCAGAGAAACGGTCACA
AGCAACACAAAAATTGTTGCGGTGTCTCATGTATCTAATGTTCTCGGCACGGTCAACCCGATTAAAGAAATGGCGAAAAT
CGCTCATGACAATGGGGCTGTCATTGTGGTGGACGGTGCACAAAGCACGCCTCACATGAAAATTGACGTGCAGGATCTCG
ATTGCGACTTCTTTGCGCTTTCTTCCCATAAAATGTGCGGACCTACCGGTGTTGGTGTGCTGTACGGAAAGAAAGCCCTG
CTGGAAAACATGGAGCCTGCCGAATTCGGCGGTGAAATGATCGACTTTGTCGGGCTTTATGAATCAACTTGGAAAGAGCT
TCCGTGGAAATTCGAAGCGGGTACGCCGATTATTGCAGGAGCAATCGGCCTCGGTGCCGCCATTGATTTTCTCGAAGAAA
TCGGTCTTGATGAGATTTCCCGCCATGAGCACAAGCTTGCAGCTTATGCGCTTGAACGCTTCCGCCAGCTTGACGGCGTA
ACGGTTTACGGGCCGGAAGAGCGTGCCGGGCTTGTCACATTTAACCTTGATGATGTTCATCCGCACGATGTGGCGACTGT
GCTTGATGCGGAGGGAATCGCAGTCAGAGCCGGCCACCATTGTGCGCAGCCGCTGATGAAATGGCTGGATGTAACTGCTA
CTGCAAGAGCAAGCTTTTATCTGTATAATACAGAGGAAGAGATTGATAAGCTTGTGGAAGCTCTTCAAAAGACAAAGGAG
TATTTTACAAATGTCTTTTAA

Upstream 100 bases:

>100_bases
GCTTAGTCATTTACGGATTCCTTGCACCGGTAGTAAATGAACTTCCGATTGAAGGCGTTAAGAAACAATTAGTTTCTGTT
ATCGAAAGGAAAGTGAAGTA

Downstream 100 bases:

>100_bases
TGCAAACTTAGATACATTGTACAGACAGGTGATTATGGATCATTACAAAAACCCGCGAAATAAAGGGGTTTTAAATGACA
GCATTGTCGTGGATATGAAC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNITDIREQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLGTRATDGYEGAREKVRKFINA
KSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYMEHHANIIPWQQAVKATGATLKYIPLQEDGTISLEDVRETVT
SNTKIVAVSHVSNVLGTVNPIKEMAKIAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHKMCGPTGVGVLYGKKAL
LENMEPAEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLGAAIDFLEEIGLDEISRHEHKLAAYALERFRQLDGV
TVYGPEERAGLVTFNLDDVHPHDVATVLDAEGIAVRAGHHCAQPLMKWLDVTATARASFYLYNTEEEIDKLVEALQKTKE
YFTNVF

Sequences:

>Translated_406_residues
MNITDIREQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLGTRATDGYEGAREKVRKFINA
KSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYMEHHANIIPWQQAVKATGATLKYIPLQEDGTISLEDVRETVT
SNTKIVAVSHVSNVLGTVNPIKEMAKIAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHKMCGPTGVGVLYGKKAL
LENMEPAEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLGAAIDFLEEIGLDEISRHEHKLAAYALERFRQLDGV
TVYGPEERAGLVTFNLDDVHPHDVATVLDAEGIAVRAGHHCAQPLMKWLDVTATARASFYLYNTEEEIDKLVEALQKTKE
YFTNVF
>Mature_406_residues
MNITDIREQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLGTRATDGYEGAREKVRKFINA
KSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYMEHHANIIPWQQAVKATGATLKYIPLQEDGTISLEDVRETVT
SNTKIVAVSHVSNVLGTVNPIKEMAKIAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHKMCGPTGVGVLYGKKAL
LENMEPAEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLGAAIDFLEEIGLDEISRHEHKLAAYALERFRQLDGV
TVYGPEERAGLVTFNLDDVHPHDVATVLDAEGIAVRAGHHCAQPLMKWLDVTATARASFYLYNTEEEIDKLVEALQKTKE
YFTNVF

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=387, Percent_Identity=26.0981912144703, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI156713448, Length=260, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1787970, Length=398, Percent_Identity=48.4924623115578, Blast_Score=420, Evalue=1e-119,
Organism=Escherichia coli, GI1789175, Length=404, Percent_Identity=41.8316831683168, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI48994898, Length=393, Percent_Identity=24.6819338422392, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25143064, Length=230, Percent_Identity=30.8695652173913, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI193211090, Length=411, Percent_Identity=26.0340632603406, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319831, Length=393, Percent_Identity=27.4809160305344, Blast_Score=129, Evalue=9e-31,
Organism=Drosophila melanogaster, GI20129463, Length=306, Percent_Identity=30.0653594771242, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSD_BACSU (O32164)

Other databases:

- EMBL:   AL009126
- PIR:   F70019
- RefSeq:   NP_391148.1
- ProteinModelPortal:   O32164
- SMR:   O32164
- EnsemblBacteria:   EBBACT00000002328
- GeneID:   937108
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32690
- NMPDR:   fig|224308.1.peg.3274
- GenoList:   BSU32690
- GeneTree:   EBGT00070000032103
- HOGENOM:   HBG635316
- OMA:   YDSTWKE
- PhylomeDB:   O32164
- ProtClustDB:   CLSK2752509
- BioCyc:   BSUB:BSU32690-MONOMER
- BRENDA:   2.8.1.7
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010970
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR01979

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 44922; Mature: 44922

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 361-361

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITDIREQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLG
CCCCHHHHHCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHC
TRATDGYEGAREKVRKFINAKSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYME
CCCCCCHHHHHHHHHHHHCHHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEH
HHANIIPWQQAVKATGATLKYIPLQEDGTISLEDVRETVTSNTKIVAVSHVSNVLGTVNP
HCCCCCCHHHHHHHCCCEEEEEEECCCCCEEHHHHHHHHCCCCEEEEHHHHHHHHHCHHH
IKEMAKIAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHKMCGPTGVGVLYGKKAL
HHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHCCHHH
LENMEPAEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLGAAIDFLEEIGLDEIS
HCCCCHHHHCCHHHHHHHHHHHHHHHCCEEECCCCCEEEHHHHHHHHHHHHHHCCHHHHH
RHEHKLAAYALERFRQLDGVTVYGPEERAGLVTFNLDDVHPHDVATVLDAEGIAVRAGHH
HHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCCEEEECCHH
CAQPLMKWLDVTATARASFYLYNTEEEIDKLVEALQKTKEYFTNVF
HHHHHHHHHCCHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNITDIREQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLG
CCCCHHHHHCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHC
TRATDGYEGAREKVRKFINAKSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYME
CCCCCCHHHHHHHHHHHHCHHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEH
HHANIIPWQQAVKATGATLKYIPLQEDGTISLEDVRETVTSNTKIVAVSHVSNVLGTVNP
HCCCCCCHHHHHHHCCCEEEEEEECCCCCEEHHHHHHHHCCCCEEEEHHHHHHHHHCHHH
IKEMAKIAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHKMCGPTGVGVLYGKKAL
HHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHCCHHH
LENMEPAEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLGAAIDFLEEIGLDEIS
HCCCCHHHHCCHHHHHHHHHHHHHHHCCEEECCCCCEEEHHHHHHHHHHHHHHCCHHHHH
RHEHKLAAYALERFRQLDGVTVYGPEERAGLVTFNLDDVHPHDVATVLDAEGIAVRAGHH
HHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCCEEEECCHH
CAQPLMKWLDVTATARASFYLYNTEEEIDKLVEALQKTKEYFTNVF
HHHHHHHHHCCHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377