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The map label for this gene is frlB

Identifier: 16080314

GI number: 16080314

Start: 3351110

End: 3352096

Strand: Reverse

Name: frlB

Synonym: BSU32610

Alternate gene names: 16080314

Gene position: 3352096-3351110 (Counterclockwise)

Preceding gene: 16080315

Following gene: 16080313

Centisome position: 79.52

GC content: 44.17

Gene sequence:

>987_bases
TTGAGTCAGGCCACAGCAAAAGTAAATCGTGAGGTTCAAGCTTTCTTGCAGGATTTGAAAGGGAAAACCATTGATCATGT
ATTCTTTGTCGCATGCGGAGGGTCTTCTGCCATTATGTATCCGAGTAAGTATGTGTTTGACAGAGAGTCAAAATCAATAA
ACTCCGATCTCTACAGCGCCAACGAATTTATTCAGCGCAACCCTGTTCAGCTTGGTGAAAAGTCTCTTGTTATTTTGTGC
TCGCATTCGGGAAATACCCCGGAGACAGTCAAAGCTGCTGCGTTTGCAAGGGGCAAGGGCGCACTTACGATTGCGATGAC
GTTTAAACCGGAGTCTCCTTTAGCGCAGGAAGCGCAATATGTTGCCCAGTACGATTGGGGTGATGAGGCGCTTGCGATCA
ATACAAACTATGGCGTTTTGTATCAAATCGTTTTTGGAACCCTGCAAGTATTAGAAAACAATACGAAATTTGAGCAAGCA
ATTGAAGGCTTAGATCAATTGCAGGCTGTATATGAAAAAGCCCTTAAGCAGGAAGCTGACAACGCCAAGCAATTTGCAAA
AGCCCATGAAAAAGAAAGCATTATTTATACCATGGCAAGCGGTGCAAACTACGGTGTCGCTTACTCCTACAGCATCTGCA
TTCTCATGGAAATGCAATGGATTCATTCCCACGCCATTCATGCCGGAGAATATTTTCACGGACCGTTTGAAATTATTGAT
GAATCCGTGCCGTTTATCATCCTGCTCGGTTTAGATGAAACAAGACCTCTTGAAGAGCGGGCGCTTACCTTCTCGAAAAA
GTATGGCAAAAAGCTCACTGTGCTTGATGCTGCATCTTACGACTTCACTGCAATTGACGATTCAGTTAAAGGCTATCTTG
CTCCGCTCGTTCTCAATCGTGTGCTGAGAAGCTATGCAGACGAGCTGGCAGAGGAGAGAAATCATCCTTTATCTCATAGA
AGATACATGTGGAAAGTTGAGTATTGA

Upstream 100 bases:

>100_bases
AAATTTCAGAAAATAATATTGACAATCCTACCCGCGGACTTTAATATAACAATATATAATGTTATATAACGTTATATAAT
AAAAACGAGGAGTGAAGGAT

Downstream 100 bases:

>100_bases
TCTGAAAATGAAGAACCATTATGCATTAGACTATAATGTTATATAACATTATAGTCTAATGTCCAGAGGAGGCCAACCCT
TTGAAGAAAATGTTGTTGTT

Product: fructoselysine-6-P-deglycase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSQATAKVNREVQAFLQDLKGKTIDHVFFVACGGSSAIMYPSKYVFDRESKSINSDLYSANEFIQRNPVQLGEKSLVILC
SHSGNTPETVKAAAFARGKGALTIAMTFKPESPLAQEAQYVAQYDWGDEALAINTNYGVLYQIVFGTLQVLENNTKFEQA
IEGLDQLQAVYEKALKQEADNAKQFAKAHEKESIIYTMASGANYGVAYSYSICILMEMQWIHSHAIHAGEYFHGPFEIID
ESVPFIILLGLDETRPLEERALTFSKKYGKKLTVLDAASYDFTAIDDSVKGYLAPLVLNRVLRSYADELAEERNHPLSHR
RYMWKVEY

Sequences:

>Translated_328_residues
MSQATAKVNREVQAFLQDLKGKTIDHVFFVACGGSSAIMYPSKYVFDRESKSINSDLYSANEFIQRNPVQLGEKSLVILC
SHSGNTPETVKAAAFARGKGALTIAMTFKPESPLAQEAQYVAQYDWGDEALAINTNYGVLYQIVFGTLQVLENNTKFEQA
IEGLDQLQAVYEKALKQEADNAKQFAKAHEKESIIYTMASGANYGVAYSYSICILMEMQWIHSHAIHAGEYFHGPFEIID
ESVPFIILLGLDETRPLEERALTFSKKYGKKLTVLDAASYDFTAIDDSVKGYLAPLVLNRVLRSYADELAEERNHPLSHR
RYMWKVEY
>Mature_327_residues
SQATAKVNREVQAFLQDLKGKTIDHVFFVACGGSSAIMYPSKYVFDRESKSINSDLYSANEFIQRNPVQLGEKSLVILCS
HSGNTPETVKAAAFARGKGALTIAMTFKPESPLAQEAQYVAQYDWGDEALAINTNYGVLYQIVFGTLQVLENNTKFEQAI
EGLDQLQAVYEKALKQEADNAKQFAKAHEKESIIYTMASGANYGVAYSYSICILMEMQWIHSHAIHAGEYFHGPFEIIDE
SVPFIILLGLDETRPLEERALTFSKKYGKKLTVLDAASYDFTAIDDSVKGYLAPLVLNRVLRSYADELAEERNHPLSHRR
YMWKVEY

Specific function: Catalyzes the conversion of a range of fructosamine 6- phosphates to glucose 6-phosphate and a free amino acid

COG id: COG2222

COG function: function code M; Predicted phosphosugar isomerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 SIS domains

Homologues:

Organism=Homo sapiens, GI4826742, Length=239, Percent_Identity=25.1046025104602, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI205277386, Length=263, Percent_Identity=22.0532319391635, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI87082251, Length=312, Percent_Identity=30.1282051282051, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1790167, Length=236, Percent_Identity=27.5423728813559, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357745, Length=237, Percent_Identity=23.2067510548523, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FRLB_BACSU (O32157)

Other databases:

- EMBL:   AL009126
- PIR:   G70018
- RefSeq:   NP_391141.1
- PDB:   3EUA
- PDBsum:   3EUA
- ProteinModelPortal:   O32157
- SMR:   O32157
- EnsemblBacteria:   EBBACT00000000576
- GeneID:   936701
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32610
- NMPDR:   fig|224308.1.peg.3267
- GenoList:   BSU32610
- GeneTree:   EBGT00050000000969
- HOGENOM:   HBG301705
- OMA:   LMEMQWI
- ProtClustDB:   CLSK887836
- BioCyc:   BSUB:BSU32610-MONOMER
- InterPro:   IPR001347

Pfam domain/function: PF01380 SIS

EC number: NA

Molecular weight: Translated: 36876; Mature: 36745

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS51464 SIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQATAKVNREVQAFLQDLKGKTIDHVFFVACGGSSAIMYPSKYVFDRESKSINSDLYSA
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCHHEECCCCCHHHHHHHHH
NEFIQRNPVQLGEKSLVILCSHSGNTPETVKAAAFARGKGALTIAMTFKPESPLAQEAQY
HHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHH
VAQYDWGDEALAINTNYGVLYQIVFGTLQVLENNTKFEQAIEGLDQLQAVYEKALKQEAD
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NAKQFAKAHEKESIIYTMASGANYGVAYSYSICILMEMQWIHSHAIHAGEYFHGPFEIID
HHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEEEEHHHHHHHHHHHCCCCCCCHHHHHC
ESVPFIILLGLDETRPLEERALTFSKKYGKKLTVLDAASYDFTAIDDSVKGYLAPLVLNR
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCEECCHHHHHHHHHHHHHH
VLRSYADELAEERNHPLSHRRYMWKVEY
HHHHHHHHHHHHHCCCCCCCEEEEEECC
>Mature Secondary Structure 
SQATAKVNREVQAFLQDLKGKTIDHVFFVACGGSSAIMYPSKYVFDRESKSINSDLYSA
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCHHEECCCCCHHHHHHHHH
NEFIQRNPVQLGEKSLVILCSHSGNTPETVKAAAFARGKGALTIAMTFKPESPLAQEAQY
HHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHH
VAQYDWGDEALAINTNYGVLYQIVFGTLQVLENNTKFEQAIEGLDQLQAVYEKALKQEAD
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NAKQFAKAHEKESIIYTMASGANYGVAYSYSICILMEMQWIHSHAIHAGEYFHGPFEIID
HHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEEEEHHHHHHHHHHHCCCCCCCHHHHHC
ESVPFIILLGLDETRPLEERALTFSKKYGKKLTVLDAASYDFTAIDDSVKGYLAPLVLNR
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCEECCHHHHHHHHHHHHHH
VLRSYADELAEERNHPLSHRRYMWKVEY
HHHHHHHHHHHHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377