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The map label for this gene is pucH

Identifier: 16080294

GI number: 16080294

Start: 3327247

End: 3328587

Strand: Reverse

Name: pucH

Synonym: BSU32410

Alternate gene names: 16080294

Gene position: 3328587-3327247 (Counterclockwise)

Preceding gene: 16080300

Following gene: 16080293

Centisome position: 78.96

GC content: 51.23

Gene sequence:

>1341_bases
ATGGCTTACGATATGGTGATAAAAGGTGCGAAGGCAGTTACGCCAGATGGCGTTATAGAAGCTGATATTGTAGTTCAAAA
CGGTGTCATTGCCGAGATAGGTTCTGACATTGAAGCAAGCGGGACGGAAATCATCCAGGCAGACGGGAAATACGTGTTTC
CTGGTGTGATAGATTGCCATGTTCATTTTAATGAACCGGGCAGGGAGGATTGGGAGGGGTTTGAAACAGGTTCTCAAATG
ATGGCCGCAGGCGGTTGCACTACCTATTTTGATATGCCGCTCAACTGTATTCCATCAACCGTGACGGCAGAGCATCTTCT
CGCAAAAGCTGAGCTAGGCAGGCAAAAATCTGCCGTGGATTTTGCGCTATGGGGCGGCCTTGTGCCAGGCCATATCGAGG
ACATCCGTCCGATGGCTGAAGCGGGAGCGATCGGGTTTAAAGCGTTTTTATCAAAGTCAGGCACTGATGAGTTCCGCTCT
GTTGATGAACGGACATTGCTCAAAGGCATGGCAGAAATCGCGGCTGCAGGAAAAATCCTCGCTCTCCACGCGGAAAGCGA
CGCGATCACAAGCTACTTGCAGATGGTTTTGGCCAATAAAGGAAAGGTGGATGCGGATGCGTACGCCGCCTCCCGCCCGG
AGGAAGCAGAAGTTGAAGCGGTGTATCGCACGATTCAGTATGCGAAGGTGACAGGCTGTCCGGTTCATTTTGTCCATATC
AGCACGGCGAAAGCGGTCCGGCTGATCAGAGAGGCGAAACAGGAAGGTCTTGATGTTTCGGTTGAAACATGTCCGCATTA
TGTCTTGTTCAGCCATGACGATTTACGGCAAAGGGGCTCTGTCGCAAAATGCGCGCCTCCGCTCCGGTCACGGCAATCAA
AAGAGACGCTGATCGAGACGCTCATTGCCGGGGACATTGACATGGTTTCCTCAGACCATTCCCCTTGCCGTCCATCCTTG
AAGCGGGAGGATAACATGTTTTTATCATGGGGCGGAATCAGCGGCGGGCAGTTCACTTTATTAGGAATGCTGGAACTGGC
GCTTGAGCATCAGATTCCATTTGAAACCATCGCAGAATGGACAGCGGCGGCCCCTGCAAAACGATTCGGCCTTCAGAAAA
AAGGGCGGCTTGAGGCGGGGTGTGATGCAGATTTCGTGCTCGTCAGCATGGAGCCGTACACTGTTACAAGAGAATCGATG
TTTGCAAAGCATAAAAAAAGCATTTACGAAGGACATACATTTCCCTGCAGCATTTCGGCCACTTACAGCAAAGGCCGGTG
CGTGTACAATGATGGGGAAAAAGTCACTGAAATTGACGGAGCTCTGGTCGTTCCTTCGTAA

Upstream 100 bases:

>100_bases
ATGTAATACGATCATAGTAGCAGTAAACTGTTGAAAATAGAACTGGTTGTGTTACATAAACTGACATGGAAAATCGTCAT
CCTGACATGGGGGGATCAAT

Downstream 100 bases:

>100_bases
GCCCGGCGGATTTTACCGCCGGGTTTTTTGATTTCCTCCGTTCTAGTTTTTAAAATCTCATCAACGTGGTATCTTTTTTT
AAAGGAGGCGGAGGCAATGG

Product: allantoinase

Products: NA

Alternate protein names: Allantoin-utilizing enzyme

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MAYDMVIKGAKAVTPDGVIEADIVVQNGVIAEIGSDIEASGTEIIQADGKYVFPGVIDCHVHFNEPGREDWEGFETGSQM
MAAGGCTTYFDMPLNCIPSTVTAEHLLAKAELGRQKSAVDFALWGGLVPGHIEDIRPMAEAGAIGFKAFLSKSGTDEFRS
VDERTLLKGMAEIAAAGKILALHAESDAITSYLQMVLANKGKVDADAYAASRPEEAEVEAVYRTIQYAKVTGCPVHFVHI
STAKAVRLIREAKQEGLDVSVETCPHYVLFSHDDLRQRGSVAKCAPPLRSRQSKETLIETLIAGDIDMVSSDHSPCRPSL
KREDNMFLSWGGISGGQFTLLGMLELALEHQIPFETIAEWTAAAPAKRFGLQKKGRLEAGCDADFVLVSMEPYTVTRESM
FAKHKKSIYEGHTFPCSISATYSKGRCVYNDGEKVTEIDGALVVPS

Sequences:

>Translated_446_residues
MAYDMVIKGAKAVTPDGVIEADIVVQNGVIAEIGSDIEASGTEIIQADGKYVFPGVIDCHVHFNEPGREDWEGFETGSQM
MAAGGCTTYFDMPLNCIPSTVTAEHLLAKAELGRQKSAVDFALWGGLVPGHIEDIRPMAEAGAIGFKAFLSKSGTDEFRS
VDERTLLKGMAEIAAAGKILALHAESDAITSYLQMVLANKGKVDADAYAASRPEEAEVEAVYRTIQYAKVTGCPVHFVHI
STAKAVRLIREAKQEGLDVSVETCPHYVLFSHDDLRQRGSVAKCAPPLRSRQSKETLIETLIAGDIDMVSSDHSPCRPSL
KREDNMFLSWGGISGGQFTLLGMLELALEHQIPFETIAEWTAAAPAKRFGLQKKGRLEAGCDADFVLVSMEPYTVTRESM
FAKHKKSIYEGHTFPCSISATYSKGRCVYNDGEKVTEIDGALVVPS
>Mature_445_residues
AYDMVIKGAKAVTPDGVIEADIVVQNGVIAEIGSDIEASGTEIIQADGKYVFPGVIDCHVHFNEPGREDWEGFETGSQMM
AAGGCTTYFDMPLNCIPSTVTAEHLLAKAELGRQKSAVDFALWGGLVPGHIEDIRPMAEAGAIGFKAFLSKSGTDEFRSV
DERTLLKGMAEIAAAGKILALHAESDAITSYLQMVLANKGKVDADAYAASRPEEAEVEAVYRTIQYAKVTGCPVHFVHIS
TAKAVRLIREAKQEGLDVSVETCPHYVLFSHDDLRQRGSVAKCAPPLRSRQSKETLIETLIAGDIDMVSSDHSPCRPSLK
REDNMFLSWGGISGGQFTLLGMLELALEHQIPFETIAEWTAAAPAKRFGLQKKGRLEAGCDADFVLVSMEPYTVTRESMF
AKHKKSIYEGHTFPCSISATYSKGRCVYNDGEKVTEIDGALVVPS

Specific function: Catalyzes the conversion of allantoin (5- ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Allantoinase subfamily

Homologues:

Organism=Homo sapiens, GI19923821, Length=452, Percent_Identity=27.4336283185841, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI4503379, Length=448, Percent_Identity=25.8928571428571, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI4503051, Length=461, Percent_Identity=26.0303687635575, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4503377, Length=467, Percent_Identity=26.7665952890792, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI62422571, Length=461, Percent_Identity=26.0303687635575, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI4503375, Length=476, Percent_Identity=25.4201680672269, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI190194363, Length=468, Percent_Identity=25, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI18105007, Length=383, Percent_Identity=25.5874673629243, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786722, Length=436, Percent_Identity=36.697247706422, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI87082175, Length=457, Percent_Identity=29.5404814004376, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71989490, Length=463, Percent_Identity=28.9416846652268, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17539558, Length=460, Percent_Identity=27.1739130434783, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI86575075, Length=440, Percent_Identity=23.8636363636364, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322218, Length=394, Percent_Identity=34.010152284264, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI18859883, Length=460, Percent_Identity=30, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=24.6113989637306, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI221377917, Length=480, Percent_Identity=26.0416666666667, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24644287, Length=319, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALLB_BACSU (O32137)

Other databases:

- EMBL:   AL009126
- PIR:   C70016
- RefSeq:   NP_391121.1
- ProteinModelPortal:   O32137
- SMR:   O32137
- EnsemblBacteria:   EBBACT00000004086
- GeneID:   936662
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32410
- NMPDR:   fig|224308.1.peg.3247
- GenoList:   BSU32410
- GeneTree:   EBGT00050000001626
- HOGENOM:   HBG724623
- OMA:   SENIAID
- PhylomeDB:   O32137
- ProtClustDB:   PRK06189
- BioCyc:   BSUB:BSU32410-MONOMER
- BRENDA:   3.5.2.5
- HAMAP:   MF_01645
- InterPro:   IPR017593
- InterPro:   IPR006680
- InterPro:   IPR011059
- TIGRFAMs:   TIGR03178

Pfam domain/function: PF01979 Amidohydro_1; SSF51338 Metalo_hydrolase

EC number: =3.5.2.5

Molecular weight: Translated: 48331; Mature: 48200

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDMVIKGAKAVTPDGVIEADIVVQNGVIAEIGSDIEASGTEIIQADGKYVFPGVIDCH
CCCCEEECCCCCCCCCCCEEEEEEEECCCCEECCCCCCCCCCEEEEECCCEECCCEEEEE
VHFNEPGREDWEGFETGSQMMAAGGCTTYFDMPLNCIPSTVTAEHLLAKAELGRQKSAVD
EEECCCCCCCCHHHHCCCHHHHCCCCCEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCHH
FALWGGLVPGHIEDIRPMAEAGAIGFKAFLSKSGTDEFRSVDERTLLKGMAEIAAAGKIL
HHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
ALHAESDAITSYLQMVLANKGKVDADAYAASRPEEAEVEAVYRTIQYAKVTGCPVHFVHI
EEECCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHEECCCEEEEEEE
STAKAVRLIREAKQEGLDVSVETCPHYVLFSHDDLRQRGSVAKCAPPLRSRQSKETLIET
CHHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHCCCCCCCCCCHHCCCHHHHHHHH
LIAGDIDMVSSDHSPCRPSLKREDNMFLSWGGISGGQFTLLGMLELALEHQIPFETIAEW
HHHCCHHEECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
TAAAPAKRFGLQKKGRLEAGCDADFVLVSMEPYTVTRESMFAKHKKSIYEGHTFPCSISA
HHCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCEECHHHHHHHHHHHHHCCCCCEEEEEE
TYSKGRCVYNDGEKVTEIDGALVVPS
ECCCCEEEECCCCEEEECCCEEECCC
>Mature Secondary Structure 
AYDMVIKGAKAVTPDGVIEADIVVQNGVIAEIGSDIEASGTEIIQADGKYVFPGVIDCH
CCCEEECCCCCCCCCCCEEEEEEEECCCCEECCCCCCCCCCEEEEECCCEECCCEEEEE
VHFNEPGREDWEGFETGSQMMAAGGCTTYFDMPLNCIPSTVTAEHLLAKAELGRQKSAVD
EEECCCCCCCCHHHHCCCHHHHCCCCCEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCHH
FALWGGLVPGHIEDIRPMAEAGAIGFKAFLSKSGTDEFRSVDERTLLKGMAEIAAAGKIL
HHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
ALHAESDAITSYLQMVLANKGKVDADAYAASRPEEAEVEAVYRTIQYAKVTGCPVHFVHI
EEECCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHEECCCEEEEEEE
STAKAVRLIREAKQEGLDVSVETCPHYVLFSHDDLRQRGSVAKCAPPLRSRQSKETLIET
CHHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHCCCCCCCCCCHHCCCHHHHHHHH
LIAGDIDMVSSDHSPCRPSLKREDNMFLSWGGISGGQFTLLGMLELALEHQIPFETIAEW
HHHCCHHEECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
TAAAPAKRFGLQKKGRLEAGCDADFVLVSMEPYTVTRESMFAKHKKSIYEGHTFPCSISA
HHCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCEECHHHHHHHHHHHHHCCCCCEEEEEE
TYSKGRCVYNDGEKVTEIDGALVVPS
ECCCCEEEECCCCEEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136