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The map label for this gene is yunD

Identifier: 16080290

GI number: 16080290

Start: 3323670

End: 3325058

Strand: Reverse

Name: yunD

Synonym: BSU32370

Alternate gene names: 16080290

Gene position: 3325058-3323670 (Counterclockwise)

Preceding gene: 16080291

Following gene: 16080289

Centisome position: 78.87

GC content: 47.44

Gene sequence:

>1389_bases
ATGAAGGAGAAGCTCCGCTTATATCATACAAATGATTTGCACAGCCATTTTGAAAACTGGCCGAAAATTGTAGATTATAT
CGAGCAGAAAAGAAAAGAGCATCAGTCAGACGGCGAAGAGACGCTCGTTTTTGATATTGGCGATCATCTTGATCGTTTTC
AATTCGTGACAGAGGCGACATTCGGAAAAGCGAATGTCGATCTCTTAAACCGCCTTCACATTGATGGAGCGGCGATCGGC
AATAACGAAGGGATTACGCTTCCGCATGAAGAGCTTGCTGCCTTATATGATCATGCTGAATTTCCTGTCATCGTCTCTAA
CCTGTTTGATAAAAATGGAAACCGTCCGTCCTGGGCGGTGCCTTACCATATCAAATCATTGAAAAACGGTATGTCCATTG
CTTTCTTAGGTGTAACTGTGCCGTATTATCCTGTATATGACAAGCTGGGATGGACAGTTACGGATGCCCTTGAAAGCATA
AAGGAAACCATTCTGGAAGTGAAAGGGCAGGCTGATATTATCGTTCTGCTGTCACATTTAGGCATTCTTGATGATCAAGC
AGTGGCTGAAGCGGTGCCTGAGATTGATGTAATCCTCGAATCACATACCCATCATTTGCTCGAAGATGGACAAGTGGTTA
ATGGGGTACTGCTGGCCAGCGCCGAGAAATACGGGCATTATGTCGGCTGTGTAGAAATCACGGTGGACAGCGTACAGCGA
AGCATCAACAGCAAAACGGCTTCTGTGCAGAACATGGCGGAATGGACAGGAGAATCCGCAGAAACTAAAGCGTTTCTCAA
TGAAAAAGAACGTGAAGCCGAGGAGAAACTGTCCGATGCCGTTGCAGAGCTTGCACAAGATGCTGAGGTGAAGTGGTTTG
AAGAATCAGAGCTGCCTTTGCTTTTGGCATACGCCTTAAAGGAATGGTGCGAGACGGACATCAGTATGGTCAATTCAGGT
GTCATTCTCGGTCCGCTAAAAGCAGGTCCTGTGACAAAGCTGGATCTGCACCGCATCTGTCCGCATCCGATCAACCCGGT
GGCTGTCCGCCTGACGGGAGAAGAACTGAAGGAAACCATTGTCCATGCCGCTTCCGAACAAATGGAGCAGCTCCGGATCA
AAGGACTCGGCTTCCGCGGAGAAGTGATGGGGAAAATGGTGTATGCCGGCGTTGAGGTAGAAACGAAACGGCTCGATGAC
GGGATCACCCACGTCACTCGGATCACACTGAACGGGGAAGATATTGAAAAACATAAACAATACTCCGTTGCGGTGCTTGA
TATGTTTACGCTCGGCAAACTGTTTCCGTTGATTCGCGACGCTGCAGAAAAAGAATATTTTATGCCGGAATTTTTGCGGG
ATCTGCTTGCTTGGAAGCTTGCACAATAA

Upstream 100 bases:

>100_bases
ACGAAAACGATCGTTATGATCATGCGGATCGTATTGATTTTGATCGGCTGCCAGCTCATTTATGAAGGCATTTTCAGCTG
AACGATTAGGGAGAGATATT

Downstream 100 bases:

>100_bases
GGCAGTCAGCCCACAACAAGCTGTGCCCTTCATCATAAAGTGATGAATGAAGGGAGTGGTTGACGTGGTAAACCTGACAC
CGATCATGATAGAAGGACAG

Product: nuclease/nucleotidase/phosphoesterase

Products: Phosphate; Uridine [C]

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKEKLRLYHTNDLHSHFENWPKIVDYIEQKRKEHQSDGEETLVFDIGDHLDRFQFVTEATFGKANVDLLNRLHIDGAAIG
NNEGITLPHEELAALYDHAEFPVIVSNLFDKNGNRPSWAVPYHIKSLKNGMSIAFLGVTVPYYPVYDKLGWTVTDALESI
KETILEVKGQADIIVLLSHLGILDDQAVAEAVPEIDVILESHTHHLLEDGQVVNGVLLASAEKYGHYVGCVEITVDSVQR
SINSKTASVQNMAEWTGESAETKAFLNEKEREAEEKLSDAVAELAQDAEVKWFEESELPLLLAYALKEWCETDISMVNSG
VILGPLKAGPVTKLDLHRICPHPINPVAVRLTGEELKETIVHAASEQMEQLRIKGLGFRGEVMGKMVYAGVEVETKRLDD
GITHVTRITLNGEDIEKHKQYSVAVLDMFTLGKLFPLIRDAAEKEYFMPEFLRDLLAWKLAQ

Sequences:

>Translated_462_residues
MKEKLRLYHTNDLHSHFENWPKIVDYIEQKRKEHQSDGEETLVFDIGDHLDRFQFVTEATFGKANVDLLNRLHIDGAAIG
NNEGITLPHEELAALYDHAEFPVIVSNLFDKNGNRPSWAVPYHIKSLKNGMSIAFLGVTVPYYPVYDKLGWTVTDALESI
KETILEVKGQADIIVLLSHLGILDDQAVAEAVPEIDVILESHTHHLLEDGQVVNGVLLASAEKYGHYVGCVEITVDSVQR
SINSKTASVQNMAEWTGESAETKAFLNEKEREAEEKLSDAVAELAQDAEVKWFEESELPLLLAYALKEWCETDISMVNSG
VILGPLKAGPVTKLDLHRICPHPINPVAVRLTGEELKETIVHAASEQMEQLRIKGLGFRGEVMGKMVYAGVEVETKRLDD
GITHVTRITLNGEDIEKHKQYSVAVLDMFTLGKLFPLIRDAAEKEYFMPEFLRDLLAWKLAQ
>Mature_462_residues
MKEKLRLYHTNDLHSHFENWPKIVDYIEQKRKEHQSDGEETLVFDIGDHLDRFQFVTEATFGKANVDLLNRLHIDGAAIG
NNEGITLPHEELAALYDHAEFPVIVSNLFDKNGNRPSWAVPYHIKSLKNGMSIAFLGVTVPYYPVYDKLGWTVTDALESI
KETILEVKGQADIIVLLSHLGILDDQAVAEAVPEIDVILESHTHHLLEDGQVVNGVLLASAEKYGHYVGCVEITVDSVQR
SINSKTASVQNMAEWTGESAETKAFLNEKEREAEEKLSDAVAELAQDAEVKWFEESELPLLLAYALKEWCETDISMVNSG
VILGPLKAGPVTKLDLHRICPHPINPVAVRLTGEELKETIVHAASEQMEQLRIKGLGFRGEVMGKMVYAGVEVETKRLDD
GITHVTRITLNGEDIEKHKQYSVAVLDMFTLGKLFPLIRDAAEKEYFMPEFLRDLLAWKLAQ

Specific function: Degradation Of External Udp-Glucose To Uridine Monophosphate And Glucose-1-Phosphate, Which Can Then Be Used By The Cell. [C]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallophosphoesterase superfamily

Homologues:

None

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YUND_BACSU (O32133)

Other databases:

- EMBL:   AL009126
- PIR:   G70015
- RefSeq:   NP_391117.1
- ProteinModelPortal:   O32133
- SMR:   O32133
- EnsemblBacteria:   EBBACT00000003924
- GeneID:   936666
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32370
- NMPDR:   fig|224308.1.peg.3243
- GenoList:   BSU32370
- GeneTree:   EBGT00070000032208
- HOGENOM:   HBG313821
- OMA:   HRICPHP
- PhylomeDB:   O32133
- ProtClustDB:   CLSK2518269
- BioCyc:   BSUB:BSU32370-MONOMER
- InterPro:   IPR008334
- InterPro:   IPR006179
- InterPro:   IPR011240
- Gene3D:   G3DSA:3.90.780.10
- PANTHER:   PTHR11575
- PANTHER:   PTHR11575:SF1
- PIRSF:   PIRSF036361
- PRINTS:   PR01607

Pfam domain/function: PF02872 5_nucleotid_C; SSF55816 5'-Nucleotdase_C

EC number: 3.6.1.45; 3.1.3.5 [C]

Molecular weight: Translated: 51872; Mature: 51872

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKLRLYHTNDLHSHFENWPKIVDYIEQKRKEHQSDGEETLVFDIGDHLDRFQFVTEAT
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHC
FGKANVDLLNRLHIDGAAIGNNEGITLPHEELAALYDHAEFPVIVSNLFDKNGNRPSWAV
CCCCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEC
PYHIKSLKNGMSIAFLGVTVPYYPVYDKLGWTVTDALESIKETILEVKGQADIIVLLSHL
CHHHHHHCCCCEEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
GILDDQAVAEAVPEIDVILESHTHHLLEDGQVVNGVLLASAEKYGHYVGCVEITVDSVQR
CCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHEECHHHHCCEEEEEEEEHHHHHH
SINSKTASVQNMAEWTGESAETKAFLNEKEREAEEKLSDAVAELAQDAEVKWFEESELPL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCH
LLAYALKEWCETDISMVNSGVILGPLKAGPVTKLDLHRICPHPINPVAVRLTGEELKETI
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEHHHHCCCCCCCCEEEEECHHHHHHHH
VHAASEQMEQLRIKGLGFRGEVMGKMVYAGVEVETKRLDDGITHVTRITLNGEDIEKHKQ
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEHHHHHCCCCEEEEEEECCCHHHHHHH
YSVAVLDMFTLGKLFPLIRDAAEKEYFMPEFLRDLLAWKLAQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEKLRLYHTNDLHSHFENWPKIVDYIEQKRKEHQSDGEETLVFDIGDHLDRFQFVTEAT
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHC
FGKANVDLLNRLHIDGAAIGNNEGITLPHEELAALYDHAEFPVIVSNLFDKNGNRPSWAV
CCCCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEC
PYHIKSLKNGMSIAFLGVTVPYYPVYDKLGWTVTDALESIKETILEVKGQADIIVLLSHL
CHHHHHHCCCCEEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
GILDDQAVAEAVPEIDVILESHTHHLLEDGQVVNGVLLASAEKYGHYVGCVEITVDSVQR
CCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHEECHHHHCCEEEEEEEEHHHHHH
SINSKTASVQNMAEWTGESAETKAFLNEKEREAEEKLSDAVAELAQDAEVKWFEESELPL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCH
LLAYALKEWCETDISMVNSGVILGPLKAGPVTKLDLHRICPHPINPVAVRLTGEELKETI
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEHHHHCCCCCCCCEEEEECHHHHHHHH
VHAASEQMEQLRIKGLGFRGEVMGKMVYAGVEVETKRLDDGITHVTRITLNGEDIEKHKQ
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEHHHHHCCCCEEEEEEECCCHHHHHHH
YSVAVLDMFTLGKLFPLIRDAAEKEYFMPEFLRDLLAWKLAQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UMP [C]

Specific reaction: H2O + UMP --> Phosphate + Uridine [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Inhibitor from Salmonella typhimurium cytoplasm [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377