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The map label for this gene is trmB

Identifier: 16080042

GI number: 16080042

Start: 3059547

End: 3060188

Strand: Reverse

Name: trmB

Synonym: BSU29900

Alternate gene names: 16080042

Gene position: 3060188-3059547 (Counterclockwise)

Preceding gene: 255767685

Following gene: 16080041

Centisome position: 72.59

GC content: 43.93

Gene sequence:

>642_bases
ATGAGAATGCGCCACAAGCCTTGGGCTGATGACTTTTTGGCTGAAAACGCAGATATTGCCATCAGCAATCCTGCTGATTA
TAAAGGGAAATGGAACACAGTGTTTGGCAACGACAATCCGATCCATATCGAGGTTGGTACAGGAAAAGGCCAATTTATTT
CTGGTATGGCCAAACAAAATCCCGACATCAATTATATTGGTATTGAGTTATTTAAAAGCGTCATCGTGACAGCCGTCCAA
AAAGTAAAAGACAGCGAAGCGCAGAATGTAAAGCTTCTGAATATAGATGCGGACACGCTGACGGATGTGTTTGAGCCGGG
GGAAGTGAAACGGGTCTACTTGAACTTTTCTGATCCTTGGCCGAAGAAACGGCATGAAAAACGCCGCCTGACATATTCCC
ACTTCCTCAAAAAATATGAAGAAGTAATGGGCAAAGGCGGATCGATTCATTTTAAAACGGACAACCGAGGTTTATTTGAG
TACTCTTTGAAGAGCTTCTCTGAGTACGGCCTCCTGCTGACATACGTCAGTCTCGATTTGCATAACAGCAATCTGGAAGG
CAATATTATGACAGAATATGAAGAAAAATTCTCTGCACTCGGCCAGCCGATCTATCGGGCTGAGGTTGAATGGAGAACGT
AA

Upstream 100 bases:

>100_bases
CGCTCGGCCTTAAGAGAGATACACTGGAAAAACGAGCTTGCGGGTCTTTTTAATTCATGGTATGTTGTTAGTCGAAATAA
TAGATAGAGGTGTCAATCAT

Downstream 100 bases:

>100_bases
AGACAGACTCTGACAGGAGTCTGTTTTTTTTATGGGCCGTTTGCTACCATGATGATAGGGGGGGGTGAAAATGGAAACAA
TGAAAATTGGAAACATCACG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: BsTrmB; tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MRMRHKPWADDFLAENADIAISNPADYKGKWNTVFGNDNPIHIEVGTGKGQFISGMAKQNPDINYIGIELFKSVIVTAVQ
KVKDSEAQNVKLLNIDADTLTDVFEPGEVKRVYLNFSDPWPKKRHEKRRLTYSHFLKKYEEVMGKGGSIHFKTDNRGLFE
YSLKSFSEYGLLLTYVSLDLHNSNLEGNIMTEYEEKFSALGQPIYRAEVEWRT

Sequences:

>Translated_213_residues
MRMRHKPWADDFLAENADIAISNPADYKGKWNTVFGNDNPIHIEVGTGKGQFISGMAKQNPDINYIGIELFKSVIVTAVQ
KVKDSEAQNVKLLNIDADTLTDVFEPGEVKRVYLNFSDPWPKKRHEKRRLTYSHFLKKYEEVMGKGGSIHFKTDNRGLFE
YSLKSFSEYGLLLTYVSLDLHNSNLEGNIMTEYEEKFSALGQPIYRAEVEWRT
>Mature_213_residues
MRMRHKPWADDFLAENADIAISNPADYKGKWNTVFGNDNPIHIEVGTGKGQFISGMAKQNPDINYIGIELFKSVIVTAVQ
KVKDSEAQNVKLLNIDADTLTDVFEPGEVKRVYLNFSDPWPKKRHEKRRLTYSHFLKKYEEVMGKGGSIHFKTDNRGLFE
YSLKSFSEYGLLLTYVSLDLHNSNLEGNIMTEYEEKFSALGQPIYRAEVEWRT

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=136, Percent_Identity=28.6764705882353, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_BACSU (O34522)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   B69997
- RefSeq:   NP_390868.1
- PDB:   2FCA
- PDBsum:   2FCA
- ProteinModelPortal:   O34522
- SMR:   O34522
- EnsemblBacteria:   EBBACT00000003255
- GeneID:   936447
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29900
- NMPDR:   fig|224308.1.peg.2993
- GenoList:   BSU29900
- GeneTree:   EBGT00050000000917
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- PhylomeDB:   O34522
- ProtClustDB:   PRK00121
- BioCyc:   BSUB:BSU29900-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 24504; Mature: 24504

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMRHKPWADDFLAENADIAISNPADYKGKWNTVFGNDNPIHIEVGTGKGQFISGMAKQN
CCCCCCCCCHHHHCCCCCEEECCCCCCCCCEEEECCCCCEEEEEEECCCCHHHCCHHHCC
PDINYIGIELFKSVIVTAVQKVKDSEAQNVKLLNIDADTLTDVFEPGEVKRVYLNFSDPW
CCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHCCCCCEEEEEEECCCCC
PKKRHEKRRLTYSHFLKKYEEVMGKGGSIHFKTDNRGLFEYSLKSFSEYGLLLTYVSLDL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEEE
HNSNLEGNIMTEYEEKFSALGQPIYRAEVEWRT
ECCCCCCCEEHHHHHHHHHHCCCEEEEEEEECC
>Mature Secondary Structure
MRMRHKPWADDFLAENADIAISNPADYKGKWNTVFGNDNPIHIEVGTGKGQFISGMAKQN
CCCCCCCCCHHHHCCCCCEEECCCCCCCCCEEEECCCCCEEEEEEECCCCHHHCCHHHCC
PDINYIGIELFKSVIVTAVQKVKDSEAQNVKLLNIDADTLTDVFEPGEVKRVYLNFSDPW
CCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHCCCCCEEEEEEECCCCC
PKKRHEKRRLTYSHFLKKYEEVMGKGGSIHFKTDNRGLFEYSLKSFSEYGLLLTYVSLDL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEEE
HNSNLEGNIMTEYEEKFSALGQPIYRAEVEWRT
ECCCCCCCEEHHHHHHHHHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377