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The map label for this gene is ccpA

Identifier: 16080026

GI number: 16080026

Start: 3044165

End: 3045169

Strand: Reverse

Name: ccpA

Synonym: BSU29740

Alternate gene names: 16080026

Gene position: 3045169-3044165 (Counterclockwise)

Preceding gene: 16080027

Following gene: 16080025

Centisome position: 72.24

GC content: 46.17

Gene sequence:

>1005_bases
ATGAGCAATATTACGATCTACGATGTAGCGAGAGAAGCTAATGTAAGCATGGCAACCGTTTCCCGTGTCGTGAACGGCAA
CCCGAATGTAAAACCGACAACGAGGAAAAAAGTCTTGGAAGCCATTGAACGTCTCGGTTACCGTCCAAACGCGGTGGCAA
GAGGGCTGGCAAGTAAAAAAACAACAACTGTAGGTGTCATCATTCCCGATATCTCAAGCATTTTCTATTCAGAGCTTGCG
CGCGGAATTGAAGATATCGCGACAATGTATAAATACAATATTATTTTGAGCAACTCTGACCAAAACATGGAGAAAGAGCT
GCACTTGTTAAACACAATGCTCGGCAAACAAGTGGACGGCATCGTGTTTATGGGCGGAAACATTACGGACGAGCATGTGG
CGGAATTTAAGCGTTCTCCAGTGCCGATTGTACTTGCCGCTTCTGTAGAAGAGCAGGAGGAAACACCGTCAGTCGCTATC
GATTACGAACAGGCGATTTATGATGCCGTGAAGCTTTTGGTTGATAAAGGACATACAGACATCGCGTTCGTTTCCGGACC
AATGGCAGAACCGATCAACCGTTCGAAAAAACTCCAAGGCTACAAACGTGCGCTTGAAGAAGCGAACCTTCCGTTTAATG
AACAATTTGTAGCTGAAGGGGATTACACATATGATTCCGGACTCGAAGCACTGCAGCATCTGATGAGCCTGGATAAAAAA
CCGACAGCCATTCTTTCTGCAACTGATGAAATGGCACTCGGCATTATCCATGCCGCTCAGGATCAGGGCTTATCCATTCC
GGAGGATCTCGACATTATCGGTTTTGATAATACAAGATTAAGCCTCATGGTTCGTCCTCAGCTTTCAACAGTTGTTCAGC
CGACATATGATATCGGCGCCGTTGCGATGAGACTGCTGACGAAGCTCATGAATAAAGAGCCGGTTGAAGAGCATATCGTC
GAACTGCCGCACCGTATAGAGCTTAGAAAGTCAACCAAGTCATAA

Upstream 100 bases:

>100_bases
TCATAAACGACATAAATTCGGACATTATGACATTTCTCTACATAAAGTGTTTATGCTATAGATAAGGATAAGTGTATCCA
GTAAAAGGAGTGGTTTTAGG

Downstream 100 bases:

>100_bases
GAAAAACAAAGAGCAAGCTTCACCTTTATGGTGAATTCTTGCTTTTTTCATGGGGAGAAATGATGAAACGTTTTGATTAT
CTTACACCTGTTGGATTTGT

Product: Lacl family transcriptional regulator

Products: NA

Alternate protein names: Glucose-resistance amylase regulator

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSNITIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKKTTTVGVIIPDISSIFYSELA
RGIEDIATMYKYNIILSNSDQNMEKELHLLNTMLGKQVDGIVFMGGNITDEHVAEFKRSPVPIVLAASVEEQEETPSVAI
DYEQAIYDAVKLLVDKGHTDIAFVSGPMAEPINRSKKLQGYKRALEEANLPFNEQFVAEGDYTYDSGLEALQHLMSLDKK
PTAILSATDEMALGIIHAAQDQGLSIPEDLDIIGFDNTRLSLMVRPQLSTVVQPTYDIGAVAMRLLTKLMNKEPVEEHIV
ELPHRIELRKSTKS

Sequences:

>Translated_334_residues
MSNITIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKKTTTVGVIIPDISSIFYSELA
RGIEDIATMYKYNIILSNSDQNMEKELHLLNTMLGKQVDGIVFMGGNITDEHVAEFKRSPVPIVLAASVEEQEETPSVAI
DYEQAIYDAVKLLVDKGHTDIAFVSGPMAEPINRSKKLQGYKRALEEANLPFNEQFVAEGDYTYDSGLEALQHLMSLDKK
PTAILSATDEMALGIIHAAQDQGLSIPEDLDIIGFDNTRLSLMVRPQLSTVVQPTYDIGAVAMRLLTKLMNKEPVEEHIV
ELPHRIELRKSTKS
>Mature_333_residues
SNITIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKKTTTVGVIIPDISSIFYSELAR
GIEDIATMYKYNIILSNSDQNMEKELHLLNTMLGKQVDGIVFMGGNITDEHVAEFKRSPVPIVLAASVEEQEETPSVAID
YEQAIYDAVKLLVDKGHTDIAFVSGPMAEPINRSKKLQGYKRALEEANLPFNEQFVAEGDYTYDSGLEALQHLMSLDKKP
TAILSATDEMALGIIHAAQDQGLSIPEDLDIIGFDNTRLSLMVRPQLSTVVQPTYDIGAVAMRLLTKLMNKEPVEEHIVE
LPHRIELRKSTKS

Specific function: Transcriptional regulator involved in both the repression of carbohydrate utilization genes such as the alpha- amylase (AmyE) and the acetyl-coenzyme A synthetase (AcsA); and in the positive regulation of genes involved in excretion of excess carbon such

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=36.0606060606061, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=33.1378299120235, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=30.7210031347962, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789068, Length=300, Percent_Identity=31.6666666666667, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1788474, Length=302, Percent_Identity=31.1258278145695, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=31.7241379310345, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787580, Length=330, Percent_Identity=30.6060606060606, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=29.8507462686567, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI48994940, Length=318, Percent_Identity=24.5283018867925, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790715, Length=326, Percent_Identity=22.3926380368098, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1787906, Length=336, Percent_Identity=25.297619047619, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786268, Length=299, Percent_Identity=25.752508361204, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789456, Length=353, Percent_Identity=25.4957507082153, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790689, Length=296, Percent_Identity=25.6756756756757, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCPA_BACSU (P25144)

Other databases:

- EMBL:   M85182
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   S15318
- RefSeq:   NP_390852.1
- PDB:   1ZVV
- PDB:   2FEP
- PDBsum:   1ZVV
- PDBsum:   2FEP
- ProteinModelPortal:   P25144
- SMR:   P25144
- EnsemblBacteria:   EBBACT00000002424
- GeneID:   935942
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29740
- NMPDR:   fig|224308.1.peg.2977
- GenoList:   BSU29740
- GeneTree:   EBGT00070000031796
- HOGENOM:   HBG753640
- OMA:   DISSIFY
- PhylomeDB:   P25144
- ProtClustDB:   CLSK2393395
- BioCyc:   BSUB:BSU29740-MONOMER
- GO:   GO:0005622
- InterPro:   IPR006377
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354
- TIGRFAMs:   TIGR01481

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 36941; Mature: 36810

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNITIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKK
CCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCC
TTTVGVIIPDISSIFYSELARGIEDIATMYKYNIILSNSDQNMEKELHLLNTMLGKQVDG
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCC
IVFMGGNITDEHVAEFKRSPVPIVLAASVEEQEETPSVAIDYEQAIYDAVKLLVDKGHTD
EEEECCCCCHHHHHHHHCCCCCEEEECCCCHHHCCCCEEECHHHHHHHHHHHHHHCCCCC
IAFVSGPMAEPINRSKKLQGYKRALEEANLPFNEQFVAEGDYTYDSGLEALQHLMSLDKK
EEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCC
PTAILSATDEMALGIIHAAQDQGLSIPEDLDIIGFDNTRLSLMVRPQLSTVVQPTYDIGA
CCEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEECCCHHHHHCCCHHHHH
VAMRLLTKLMNKEPVEEHIVELPHRIELRKSTKS
HHHHHHHHHHCCCCHHHHHHHCCHHEEHHHCCCC
>Mature Secondary Structure 
SNITIYDVAREANVSMATVSRVVNGNPNVKPTTRKKVLEAIERLGYRPNAVARGLASKK
CCEEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCC
TTTVGVIIPDISSIFYSELARGIEDIATMYKYNIILSNSDQNMEKELHLLNTMLGKQVDG
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCC
IVFMGGNITDEHVAEFKRSPVPIVLAASVEEQEETPSVAIDYEQAIYDAVKLLVDKGHTD
EEEECCCCCHHHHHHHHCCCCCEEEECCCCHHHCCCCEEECHHHHHHHHHHHHHHCCCCC
IAFVSGPMAEPINRSKKLQGYKRALEEANLPFNEQFVAEGDYTYDSGLEALQHLMSLDKK
EEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCC
PTAILSATDEMALGIIHAAQDQGLSIPEDLDIIGFDNTRLSLMVRPQLSTVVQPTYDIGA
CCEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEECCCHHHHHCCCHHHHH
VAMRLLTKLMNKEPVEEHIVELPHRIELRKSTKS
HHHHHHHHHHCCCCHHHHHHHCCHHEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904524; 9387221; 9384377