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The map label for this gene is tyrS

Identifier: 16080019

GI number: 16080019

Start: 3036603

End: 3037871

Strand: Reverse

Name: tyrS

Synonym: BSU29670

Alternate gene names: 16080019

Gene position: 3037871-3036603 (Counterclockwise)

Preceding gene: 255767681

Following gene: 16080017

Centisome position: 72.06

GC content: 44.29

Gene sequence:

>1269_bases
ATGACTAACTTACTTGAAGACTTATCCTTCCGCGGATTGATTCAGCAAATGACAGATGAAGAAGGGTTAAATAAACAGCT
GAATGAAGAAAAAATCCGCTTGTACTCAGGCTTTGACCCTACAGCAGACAGCTTGCATATCGGACACCTGCTGCCTATTT
TAACACTTCGCCGTTTTCAGCTTGCAGGGCATCATCCGATTGCGCTTGTGGGCGGCGCGACGGGCCTCATCGGTGATCCA
AGCGGAAAAAAAGCAGAGCGTACATTAAACACTGCTGACATCGTATCCGAATGGTCCCAAAAAATCAAAAACCAGCTGTC
CAGATTTCTAGATTTTGAAGCAGCAGAAAACCCAGCTGTCATCGCGAACAACTTTGACTGGATCGGCAAAATGAATGTGA
TCGATTTCCTTCGTGACGTTGGTAAAAATTTCGGCATCAATTACATGCTGGCAAAAGATACGGTCAGCTCAAGAATTGAA
TCCGGCATCTCGTACACGGAATTCAGCTACATGATTCTTCAATCTTATGATTTCTTAAATCTTTACAGAGACAAAAACTG
TAAACTGCAAATCGGCGGAAGCGATCAGTGGGGCAACATCACAGCGGGTCTTGAACTGATCAGAAAATCAGAAGAAGAAG
GAGCAAAAGCGTTTGGCCTAACGATTCCGCTTGTCACAAAAGCAGACGGCACGAAGTTCGGAAAAACGGAAGGCGGCGCG
ATCTGGCTTGATAAAGAAAAAACATCGCCATATGAATTCTATCAATTCTGGATCAACACAGACGACCGTGACGTTGTCAA
ATACTTGAAATACTTCACGTTCCTTTCAAAAGAGGAAATTGAAGCATACGCTGAAAAAACAGAAACGGCGCCAGAAAAAC
GTGAAGCGCAAAAACGCCTGGCAGAAGAAGTGACATCACTTGTTCACGGACGCGAGGCGCTGGAGCAAGCCATCAACATC
TCCCAAGCGCTGTTCAGCGGCAATATTAAAGAGCTTTCTGCTCAAGACGTAAAAGTAGGCTTTAAAGATGTTCCTTCTAT
GGAAGTTGACAGCACCCAAGAGCTTTCACTTGTAGATGTGTTGGTGCAATCTAAATTATCTCCTTCTAAACGCCAAGCGC
GTGAAGACATCCAAAACGGAGCTGTTTACATTAACGGTGAACGCCAGACGGAAATAAATTATACCCTATCAGGTGAAGAC
CGCATCGAAAACCAATTTACTGTTCTGCGCCGCGGGAAGAAAAAATACTTCCTTGTGACGTATAAATAA

Upstream 100 bases:

>100_bases
TGCATTCTCTTATTAGTAGGGTGGTACCGCGATAATCAATCGTCCCTTCGTGTAAACGAAGGGGCGTTTTTTATTTTAAT
TAAAAAAGGAGCTTTATCTT

Downstream 100 bases:

>100_bases
GAAAAAGATCCTTTGCCACTGAAGGCAAAGGATCTTTTTGTTTACCGCATAGGAATGAAAGGAAAAGAGTATATTAGATA
GGGCAAACACGACCAATCAC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 1; TyrRS 1

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTNLLEDLSFRGLIQQMTDEEGLNKQLNEEKIRLYSGFDPTADSLHIGHLLPILTLRRFQLAGHHPIALVGGATGLIGDP
SGKKAERTLNTADIVSEWSQKIKNQLSRFLDFEAAENPAVIANNFDWIGKMNVIDFLRDVGKNFGINYMLAKDTVSSRIE
SGISYTEFSYMILQSYDFLNLYRDKNCKLQIGGSDQWGNITAGLELIRKSEEEGAKAFGLTIPLVTKADGTKFGKTEGGA
IWLDKEKTSPYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEAYAEKTETAPEKREAQKRLAEEVTSLVHGREALEQAINI
SQALFSGNIKELSAQDVKVGFKDVPSMEVDSTQELSLVDVLVQSKLSPSKRQAREDIQNGAVYINGERQTEINYTLSGED
RIENQFTVLRRGKKKYFLVTYK

Sequences:

>Translated_422_residues
MTNLLEDLSFRGLIQQMTDEEGLNKQLNEEKIRLYSGFDPTADSLHIGHLLPILTLRRFQLAGHHPIALVGGATGLIGDP
SGKKAERTLNTADIVSEWSQKIKNQLSRFLDFEAAENPAVIANNFDWIGKMNVIDFLRDVGKNFGINYMLAKDTVSSRIE
SGISYTEFSYMILQSYDFLNLYRDKNCKLQIGGSDQWGNITAGLELIRKSEEEGAKAFGLTIPLVTKADGTKFGKTEGGA
IWLDKEKTSPYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEAYAEKTETAPEKREAQKRLAEEVTSLVHGREALEQAINI
SQALFSGNIKELSAQDVKVGFKDVPSMEVDSTQELSLVDVLVQSKLSPSKRQAREDIQNGAVYINGERQTEINYTLSGED
RIENQFTVLRRGKKKYFLVTYK
>Mature_421_residues
TNLLEDLSFRGLIQQMTDEEGLNKQLNEEKIRLYSGFDPTADSLHIGHLLPILTLRRFQLAGHHPIALVGGATGLIGDPS
GKKAERTLNTADIVSEWSQKIKNQLSRFLDFEAAENPAVIANNFDWIGKMNVIDFLRDVGKNFGINYMLAKDTVSSRIES
GISYTEFSYMILQSYDFLNLYRDKNCKLQIGGSDQWGNITAGLELIRKSEEEGAKAFGLTIPLVTKADGTKFGKTEGGAI
WLDKEKTSPYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEAYAEKTETAPEKREAQKRLAEEVTSLVHGREALEQAINIS
QALFSGNIKELSAQDVKVGFKDVPSMEVDSTQELSLVDVLVQSKLSPSKRQAREDIQNGAVYINGERQTEINYTLSGEDR
IENQFTVLRRGKKKYFLVTYK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=441, Percent_Identity=38.3219954648526, Blast_Score=270, Evalue=3e-72,
Organism=Escherichia coli, GI1787925, Length=425, Percent_Identity=54.8235294117647, Blast_Score=477, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25145802, Length=432, Percent_Identity=35.1851851851852, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI25145805, Length=333, Percent_Identity=33.033033033033, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=36.986301369863, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI20130383, Length=408, Percent_Identity=41.9117647058824, Blast_Score=327, Evalue=1e-89,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY1_BACSU (P22326)

Other databases:

- EMBL:   M77668
- EMBL:   M59358
- EMBL:   AF008220
- EMBL:   AL009126
- EMBL:   L17309
- PIR:   A42648
- RefSeq:   NP_390845.1
- ProteinModelPortal:   P22326
- SMR:   P22326
- EnsemblBacteria:   EBBACT00000002500
- GeneID:   938185
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29670
- NMPDR:   fig|224308.1.peg.2970
- GenoList:   BSU29670
- GeneTree:   EBGT00050000000599
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- PhylomeDB:   P22326
- ProtClustDB:   PRK05912
- BioCyc:   BSUB:BSU29670-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47738; Mature: 47606

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 35-35 BINDING 170-170 BINDING 174-174 BINDING 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLLEDLSFRGLIQQMTDEEGLNKQLNEEKIRLYSGFDPTADSLHIGHLLPILTLRRFQ
CCCHHHHHHHHHHHHHHHCHHCCHHHCCCCCEEEECCCCCCCCCEEHHHHHHHHHHHHHH
LAGHHPIALVGGATGLIGDPSGKKAERTLNTADIVSEWSQKIKNQLSRFLDFEAAENPAV
HCCCCCEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IANNFDWIGKMNVIDFLRDVGKNFGINYMLAKDTVSSRIESGISYTEFSYMILQSYDFLN
EECCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHCHHHH
LYRDKNCKLQIGGSDQWGNITAGLELIRKSEEEGAKAFGLTIPLVTKADGTKFGKTEGGA
HHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCE
IWLDKEKTSPYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEAYAEKTETAPEKREAQKRL
EEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AEEVTSLVHGREALEQAINISQALFSGNIKELSAQDVKVGFKDVPSMEVDSTQELSLVDV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHCCHHCCCCCCCCCCCHHHHHHH
LVQSKLSPSKRQAREDIQNGAVYINGERQTEINYTLSGEDRIENQFTVLRRGKKKYFLVT
HHHHCCCCHHHHHHHHHHCCEEEECCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEE
YK
EC
>Mature Secondary Structure 
TNLLEDLSFRGLIQQMTDEEGLNKQLNEEKIRLYSGFDPTADSLHIGHLLPILTLRRFQ
CCHHHHHHHHHHHHHHHCHHCCHHHCCCCCEEEECCCCCCCCCEEHHHHHHHHHHHHHH
LAGHHPIALVGGATGLIGDPSGKKAERTLNTADIVSEWSQKIKNQLSRFLDFEAAENPAV
HCCCCCEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IANNFDWIGKMNVIDFLRDVGKNFGINYMLAKDTVSSRIESGISYTEFSYMILQSYDFLN
EECCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHCHHHH
LYRDKNCKLQIGGSDQWGNITAGLELIRKSEEEGAKAFGLTIPLVTKADGTKFGKTEGGA
HHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCE
IWLDKEKTSPYEFYQFWINTDDRDVVKYLKYFTFLSKEEIEAYAEKTETAPEKREAQKRL
EEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AEEVTSLVHGREALEQAINISQALFSGNIKELSAQDVKVGFKDVPSMEVDSTQELSLVDV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHCCHHCCCCCCCCCCCHHHHHHH
LVQSKLSPSKRQAREDIQNGAVYINGERQTEINYTLSGEDRIENQFTVLRRGKKKYFLVT
HHHHCCCCHHHHHHHHHHCCEEEECCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEE
YK
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1735721; 9387221; 9384377; 1699930