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The map label for this gene is sppA

Identifier: 16080005

GI number: 16080005

Start: 3020040

End: 3021047

Strand: Reverse

Name: sppA

Synonym: BSU29530

Alternate gene names: 16080005

Gene position: 3021047-3020040 (Counterclockwise)

Preceding gene: 16080007

Following gene: 16080004

Centisome position: 71.66

GC content: 44.35

Gene sequence:

>1008_bases
ATGAATGCAAAAAGATGGATTGCATTAGTGATTGCTCTGGGGATTTTCGGCGTGTCTATTATCGTCAGCATCTCTATGAG
TTTCTTTGAAAGCGTCAAAGGCGCTCAAACGGATCTCACATCACTGACGGATGAATCGCAGGAGAAGACGCTGGAAAACG
GCAGTCCCTCAAGTAAAATTGCCGTATTAGAGGTCAGCGGCACCATTCAGGATAATGGGGACTCAAGCAGTCTGCTTGGT
GCAGACGGATATAACCACAGAACGTTCTTAAAAAACCTTGAGCGCGCAAAAGATGACAAGACGGTCAAAGGTATCGTTCT
GAAGGTGAATTCTCCGGGTGGCGGAGTGTATGAAAGTGCTGAAATACATAAGAAACTGGAAGAAATCAAGAAAGAAACGA
AAAAACCGATTTACGTGTCAATGGGTTCGATGGCAGCATCAGGAGGCTATTACATCTCAACAGCGGCTGATAAGATTTTT
GCGACACCGGAAACCCTGACCGGGTCACTCGGCGTCATTATGGAAAGCGTCAATTATTCAAAGCTTGCCGACAAGCTTGG
CATTTCTTTTGAAACGATTAAGAGCGGGGCCCATAAGGACATTATGTCTCCTTCCCGTGAGATGACGAAAGAAGAAAAAA
ACATCATGCAATCGATGGTTGATAATTCGTATGAAGGCTTTGTTGATGTCATTTCAAAAGGGCGCGGCATGCCGAAAGCA
GAGGTAAAGAAAATTGCGGACGGCCGCGTGTATGACGGACGGCAGGCGAAAAAACTGAACCTCGTTGATGAGCTTGGTTT
TTATGACGATACCATTACGGCCATGAAAAAGGATCACAAGGATTTGAAAAACGCCTCTGTCATTTCTTATGAGGAAAGCT
TCGGATTAGGCTCACTGTTTTCCATGGGCGCGAACAAAATGTTTAAAAGTGAAATTGATTTTTTGAATATGAGAGAAATT
CTATCGCAATCCGGTTCGCCGAGAATGATGTATCTCTATGCGAAGTAG

Upstream 100 bases:

>100_bases
GTTTGCCGGCAGCCCGTTAAGCCGGAGCTTTCTCATGAAGTGAAACATTTTTCATATTGAATCGTATAATGAGAGAGTTT
AGGAAAAAGGAGGAGAAAAA

Downstream 100 bases:

>100_bases
GAGGGAACAATAATGGACGCGACATATGAAGAGTTAGAGCGCAATGACATCAAAGGCCCGCAAGAAGCGGAGCTGCTGAC
ACATGCCTATGCTGGCTTTT

Product: signal peptide peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNAKRWIALVIALGIFGVSIIVSISMSFFESVKGAQTDLTSLTDESQEKTLENGSPSSKIAVLEVSGTIQDNGDSSSLLG
ADGYNHRTFLKNLERAKDDKTVKGIVLKVNSPGGGVYESAEIHKKLEEIKKETKKPIYVSMGSMAASGGYYISTAADKIF
ATPETLTGSLGVIMESVNYSKLADKLGISFETIKSGAHKDIMSPSREMTKEEKNIMQSMVDNSYEGFVDVISKGRGMPKA
EVKKIADGRVYDGRQAKKLNLVDELGFYDDTITAMKKDHKDLKNASVISYEESFGLGSLFSMGANKMFKSEIDFLNMREI
LSQSGSPRMMYLYAK

Sequences:

>Translated_335_residues
MNAKRWIALVIALGIFGVSIIVSISMSFFESVKGAQTDLTSLTDESQEKTLENGSPSSKIAVLEVSGTIQDNGDSSSLLG
ADGYNHRTFLKNLERAKDDKTVKGIVLKVNSPGGGVYESAEIHKKLEEIKKETKKPIYVSMGSMAASGGYYISTAADKIF
ATPETLTGSLGVIMESVNYSKLADKLGISFETIKSGAHKDIMSPSREMTKEEKNIMQSMVDNSYEGFVDVISKGRGMPKA
EVKKIADGRVYDGRQAKKLNLVDELGFYDDTITAMKKDHKDLKNASVISYEESFGLGSLFSMGANKMFKSEIDFLNMREI
LSQSGSPRMMYLYAK
>Mature_335_residues
MNAKRWIALVIALGIFGVSIIVSISMSFFESVKGAQTDLTSLTDESQEKTLENGSPSSKIAVLEVSGTIQDNGDSSSLLG
ADGYNHRTFLKNLERAKDDKTVKGIVLKVNSPGGGVYESAEIHKKLEEIKKETKKPIYVSMGSMAASGGYYISTAADKIF
ATPETLTGSLGVIMESVNYSKLADKLGISFETIKSGAHKDIMSPSREMTKEEKNIMQSMVDNSYEGFVDVISKGRGMPKA
EVKKIADGRVYDGRQAKKLNLVDELGFYDDTITAMKKDHKDLKNASVISYEESFGLGSLFSMGANKMFKSEIDFLNMREI
LSQSGSPRMMYLYAK

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family

Homologues:

Organism=Escherichia coli, GI1788064, Length=213, Percent_Identity=36.150234741784, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787527, Length=171, Percent_Identity=28.0701754385965, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPPA_BACSU (O34525)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   E69990
- RefSeq:   NP_390831.1
- ProteinModelPortal:   O34525
- SMR:   O34525
- MEROPS:   S49.001
- EnsemblBacteria:   EBBACT00000000405
- GeneID:   937679
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29530
- NMPDR:   fig|224308.1.peg.2956
- GenoList:   BSU29530
- GeneTree:   EBGT00050000001875
- HOGENOM:   HBG294245
- OMA:   YDGRQAK
- ProtClustDB:   CLSK873537
- BioCyc:   BSUB:BSU29530-MONOMER
- GO:   GO:0006508
- InterPro:   IPR004635
- InterPro:   IPR002142
- TIGRFAMs:   TIGR00706

Pfam domain/function: PF01343 Peptidase_S49

EC number: 3.4.21.-

Molecular weight: Translated: 36673; Mature: 36673

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: ACT_SITE 147-147

Signals:

None

Transmembrane regions:

HASH(0x108cfdf0)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKRWIALVIALGIFGVSIIVSISMSFFESVKGAQTDLTSLTDESQEKTLENGSPSSKI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCEE
AVLEVSGTIQDNGDSSSLLGADGYNHRTFLKNLERAKDDKTVKGIVLKVNSPGGGVYESA
EEEEEECEEECCCCCCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHH
EIHKKLEEIKKETKKPIYVSMGSMAASGGYYISTAADKIFATPETLTGSLGVIMESVNYS
HHHHHHHHHHHHHCCCEEEEECCEECCCCEEEEECCCHHCCCCHHHCCHHHHHHHHCCHH
KLADKLGISFETIKSGAHKDIMSPSREMTKEEKNIMQSMVDNSYEGFVDVISKGRGMPKA
HHHHHHCCCHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHH
EVKKIADGRVYDGRQAKKLNLVDELGFYDDTITAMKKDHKDLKNASVISYEESFGLGSLF
HHHHHHCCCCCCCCCCHHCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEHHHCCCHHHH
SMGANKMFKSEIDFLNMREILSQSGSPRMMYLYAK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEH
>Mature Secondary Structure
MNAKRWIALVIALGIFGVSIIVSISMSFFESVKGAQTDLTSLTDESQEKTLENGSPSSKI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCEE
AVLEVSGTIQDNGDSSSLLGADGYNHRTFLKNLERAKDDKTVKGIVLKVNSPGGGVYESA
EEEEEECEEECCCCCCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHH
EIHKKLEEIKKETKKPIYVSMGSMAASGGYYISTAADKIFATPETLTGSLGVIMESVNYS
HHHHHHHHHHHHHCCCEEEEECCEECCCCEEEEECCCHHCCCCHHHCCHHHHHHHHCCHH
KLADKLGISFETIKSGAHKDIMSPSREMTKEEKNIMQSMVDNSYEGFVDVISKGRGMPKA
HHHHHHCCCHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHH
EVKKIADGRVYDGRQAKKLNLVDELGFYDDTITAMKKDHKDLKNASVISYEESFGLGSLF
HHHHHHCCCCCCCCCCHHCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEHHHCCCHHHH
SMGANKMFKSEIDFLNMREILSQSGSPRMMYLYAK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123