Click here to switch to the map view.

The map label for this gene is tpx

Identifier: 16080001

GI number: 16080001

Start: 3017696

End: 3018199

Strand: Reverse

Name: tpx

Synonym: BSU29490

Alternate gene names: 16080001

Gene position: 3018199-3017696 (Counterclockwise)

Preceding gene: 16080002

Following gene: 16080000

Centisome position: 71.6

GC content: 44.44

Gene sequence:

>504_bases
ATGGCTGAAATTACATTCAAAGGCGGTCCGGTTACACTTGTCGGACAAGAAGTGAAGGTAGGAGATCAAGCTCCTGATTT
CACAGTGCTGACTAACAGCCTTGAAGAAAAATCGTTAGCTGACATGAAAGGAAAGGTAACGATTATTTCTGTTATCCCTT
CAATTGATACAGGTGTTTGTGATGCACAAACACGCCGTTTTAACGAAGAAGCTGCAAAGCTTGGAGATGTCAACGTCTAC
ACAATCAGTGCAGACCTTCCATTCGCGCAAGCGCGCTGGTGCGGCGCTAACGGAATTGACAAAGTGGAAACACTGTCTGA
CCACAGAGATATGTCATTTGGCGAAGCGTTCGGTGTGTATATTAAAGAACTTCGTCTGCTTGCGCGTTCCGTATTTGTCC
TTGATGAGAACGGAAAAGTCGTTTACGCTGAATATGTAAGCGAAGCAACAAACCACCCGAACTATGAAAAGCCAATTGAG
GCTGCTAAGGCATTAGTAAAGTAA

Upstream 100 bases:

>100_bases
AACTGCGCTGGCCGCAGTTAACTTTTTTTTGGCATAAACTTTGCAGTTTGCAGGAAACTTTACTATGATAGAGACATACA
TACAAAAGGGAGGAATTATA

Downstream 100 bases:

>100_bases
GCAGGGAAAAAAGCTCCAGGCTGCCGCCGGAGCTTTTTTCATGGCAAGGAGGAACACAATATGCAAAAAGACCATGTAGG
TGCGGTGTATGAGCTGTTAA

Product: thiol peroxidase

Products: oxidized thioredoxin; H2O [C]

Alternate protein names: Superoxide-inducible protein 8; SOI8

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MAEITFKGGPVTLVGQEVKVGDQAPDFTVLTNSLEEKSLADMKGKVTIISVIPSIDTGVCDAQTRRFNEEAAKLGDVNVY
TISADLPFAQARWCGANGIDKVETLSDHRDMSFGEAFGVYIKELRLLARSVFVLDENGKVVYAEYVSEATNHPNYEKPIE
AAKALVK

Sequences:

>Translated_167_residues
MAEITFKGGPVTLVGQEVKVGDQAPDFTVLTNSLEEKSLADMKGKVTIISVIPSIDTGVCDAQTRRFNEEAAKLGDVNVY
TISADLPFAQARWCGANGIDKVETLSDHRDMSFGEAFGVYIKELRLLARSVFVLDENGKVVYAEYVSEATNHPNYEKPIE
AAKALVK
>Mature_166_residues
AEITFKGGPVTLVGQEVKVGDQAPDFTVLTNSLEEKSLADMKGKVTIISVIPSIDTGVCDAQTRRFNEEAAKLGDVNVYT
ISADLPFAQARWCGANGIDKVETLSDHRDMSFGEAFGVYIKELRLLARSVFVLDENGKVVYAEYVSEATNHPNYEKPIEA
AKALVK

Specific function: Has antioxidant activity. Could remove peroxides or H(2)O(2)

COG id: COG2077

COG function: function code O; Peroxiredoxin

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain

Homologues:

Organism=Escherichia coli, GI1787584, Length=164, Percent_Identity=42.0731707317073, Blast_Score=137, Evalue=3e-34,

Paralogues:

None

Copy number: 2480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPX_BACSU (P80864)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   F69992
- RefSeq:   NP_390827.1
- PDB:   2JSY
- PDB:   2JSZ
- PDBsum:   2JSY
- PDBsum:   2JSZ
- ProteinModelPortal:   P80864
- SMR:   P80864
- EnsemblBacteria:   EBBACT00000002544
- GeneID:   937688
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29490
- NMPDR:   fig|224308.1.peg.2952
- GenoList:   BSU29490
- GeneTree:   EBGT00050000002207
- HOGENOM:   HBG493509
- OMA:   VRKFNAQ
- PhylomeDB:   P80864
- ProtClustDB:   PRK00522
- BioCyc:   BSUB:BSU29490-MONOMER
- HAMAP:   MF_00269
- InterPro:   IPR018219
- InterPro:   IPR002065
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- PANTHER:   PTHR10681:SF3

Pfam domain/function: PF08534 Redoxin; SSF52833 Thiordxn-like_fd

EC number: 1.11.1.- [C]

Molecular weight: Translated: 18216; Mature: 18085

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS51352 THIOREDOXIN_2; PS01265 TPX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITFKGGPVTLVGQEVKVGDQAPDFTVLTNSLEEKSLADMKGKVTIISVIPSIDTGVC
CCEEEECCCCEEEEECEEECCCCCCCCEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCC
DAQTRRFNEEAAKLGDVNVYTISADLPFAQARWCGANGIDKVETLSDHRDMSFGEAFGVY
CHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHH
IKELRLLARSVFVLDENGKVVYAEYVSEATNHPNYEKPIEAAKALVK
HHHHHHHHHHEEEECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AEITFKGGPVTLVGQEVKVGDQAPDFTVLTNSLEEKSLADMKGKVTIISVIPSIDTGVC
CEEEECCCCEEEEECEEECCCCCCCCEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCC
DAQTRRFNEEAAKLGDVNVYTISADLPFAQARWCGANGIDKVETLSDHRDMSFGEAFGVY
CHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHH
IKELRLLARSVFVLDENGKVVYAEYVSEATNHPNYEKPIEAAKALVK
HHHHHHHHHHEEEECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced thioredoxin; H2O2 [C]

Specific reaction: reduced thioredoxin + H2O2 = oxidized thioredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 9298659