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The map label for this gene is tcyJ

Identifier: 16079990

GI number: 16079990

Start: 3007426

End: 3008235

Strand: Reverse

Name: tcyJ

Synonym: BSU29380

Alternate gene names: 16079990

Gene position: 3008235-3007426 (Counterclockwise)

Preceding gene: 16079991

Following gene: 16079989

Centisome position: 71.36

GC content: 40.49

Gene sequence:

>810_bases
ATGAATAAGCGTAAAGGATTGGTTTTGCTGCTGTCAGTGTTTGCATTACTTGGCGGGGGATGTTCGCAAACAAATAACAA
AACAGATCGTCAGGCTCAAACGGTCATAGTCGGCACTGGAACAGATTTTCCGAATATTGCGTTTTTAAATGAAAAAGGTG
AATTGACCGGGTATGACATTGAAGTGATGAAAGCCATCGATAAGGAGTTGCCTCAATATACATTTGAATTTAAAACGATG
GATTTCTCAAATTTGCTGACGAGTCTCGGAAATAAAAAAATTGATGTGATTGCGCACAATATGGCCAAAAATAAAGAACG
AGAAAAGCGTTTTTTGTATCATAAAGTCCCATATAACTACTCGCCGATGTACATCACGGTGAAAGAGGATAATCACAAGA
TTCATACATTAAAAGACTTACACGGAAAAACAGTCATAGTTGGCGCCACGTCAAATGCGGCAGATTATATTACAAAGTAC
AACAAAACGCACGGCAGCCCAATTCATTTGAAATATGCGGGACAGGGAAGCAATGATACCGCCAATCAAATTGAAACCGG
GCGCGCAGATGCAACGATAGCTACACCGTTTGCCGTCGATTTTCAGAATAAAACGCACGCCTTCCGCCAAAAGACAGTCG
GTGACGTGTTATTAGATACCGAGGTGTACTTTATGTTTAATAAAGGCAGCCAAACCTTGGCGGATGACACGGATCAAGCG
ATTAAAAAGCTGGAGAAAAACGGAACGCTAAAAAAACTAAGCCGAAAATGGCTCGGGGCAGATTATTCAAAATCAAGTTT
TGAAAAATAA

Upstream 100 bases:

>100_bases
GACGGGGACATAGGTGTGATGATGCACAAGGTTCTGATTCCGGAACGATTTAACCCAACGCTTTTAGGTGCGCCCAGCTG
GGCATGATGTGAGGCAGCCT

Downstream 100 bases:

>100_bases
ATGGGGCGATGAGATGAAAACGAAAACAGCATTCATGGCAATATTATTTTCGCTTATAACAGTTCTGTCAGCTTGCGGTG
CAGGCAGTCAAACGACGGGA

Product: sulfur containing amino acid ABC transporter binding lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNKRKGLVLLLSVFALLGGGCSQTNNKTDRQAQTVIVGTGTDFPNIAFLNEKGELTGYDIEVMKAIDKELPQYTFEFKTM
DFSNLLTSLGNKKIDVIAHNMAKNKEREKRFLYHKVPYNYSPMYITVKEDNHKIHTLKDLHGKTVIVGATSNAADYITKY
NKTHGSPIHLKYAGQGSNDTANQIETGRADATIATPFAVDFQNKTHAFRQKTVGDVLLDTEVYFMFNKGSQTLADDTDQA
IKKLEKNGTLKKLSRKWLGADYSKSSFEK

Sequences:

>Translated_269_residues
MNKRKGLVLLLSVFALLGGGCSQTNNKTDRQAQTVIVGTGTDFPNIAFLNEKGELTGYDIEVMKAIDKELPQYTFEFKTM
DFSNLLTSLGNKKIDVIAHNMAKNKEREKRFLYHKVPYNYSPMYITVKEDNHKIHTLKDLHGKTVIVGATSNAADYITKY
NKTHGSPIHLKYAGQGSNDTANQIETGRADATIATPFAVDFQNKTHAFRQKTVGDVLLDTEVYFMFNKGSQTLADDTDQA
IKKLEKNGTLKKLSRKWLGADYSKSSFEK
>Mature_269_residues
MNKRKGLVLLLSVFALLGGGCSQTNNKTDRQAQTVIVGTGTDFPNIAFLNEKGELTGYDIEVMKAIDKELPQYTFEFKTM
DFSNLLTSLGNKKIDVIAHNMAKNKEREKRFLYHKVPYNYSPMYITVKEDNHKIHTLKDLHGKTVIVGATSNAADYITKY
NKTHGSPIHLKYAGQGSNDTANQIETGRADATIATPFAVDFQNKTHAFRQKTVGDVLLDTEVYFMFNKGSQTLADDTDQA
IKKLEKNGTLKKLSRKWLGADYSKSSFEK

Specific function: Part of the ABC transporter complex tcyJKLMN involved in L-cystine import. Is also involved in cystathionine, djenkolate, and S-methylcysteine transport

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family

Homologues:

Organism=Escherichia coli, GI1788228, Length=243, Percent_Identity=28.8065843621399, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787031, Length=230, Percent_Identity=32.1739130434783, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TCYJ_BACSU (O34406)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   D69996
- RefSeq:   NP_390816.1
- ProteinModelPortal:   O34406
- SMR:   O34406
- EnsemblBacteria:   EBBACT00000003354
- GeneID:   937354
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29380
- NMPDR:   fig|224308.1.peg.2941
- GenoList:   BSU29380
- GeneTree:   EBGT00070000032071
- HOGENOM:   HBG698334
- OMA:   ACENQAT
- ProtClustDB:   CLSK887717
- BioCyc:   BSUB:BSU29380-MONOMER
- InterPro:   IPR001638
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3

EC number: NA

Molecular weight: Translated: 30175; Mature: 30175

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS01039 SBP_BACTERIAL_3; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRKGLVLLLSVFALLGGGCSQTNNKTDRQAQTVIVGTGTDFPNIAFLNEKGELTGYDI
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCHH
EVMKAIDKELPQYTFEFKTMDFSNLLTSLGNKKIDVIAHNMAKNKEREKRFLYHKVPYNY
HHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCEEEEEEHHHHHCHHHHHHEEEEECCCCC
SPMYITVKEDNHKIHTLKDLHGKTVIVGATSNAADYITKYNKTHGSPIHLKYAGQGSNDT
CCEEEEEECCCCEEEEECCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCC
ANQIETGRADATIATPFAVDFQNKTHAFRQKTVGDVLLDTEVYFMFNKGSQTLADDTDQA
HHHHHCCCCCCEEECCEEEECCCCHHHHHHHHHCCEEECCEEEEEECCCCCCCCCCHHHH
IKKLEKNGTLKKLSRKWLGADYSKSSFEK
HHHHHHCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNKRKGLVLLLSVFALLGGGCSQTNNKTDRQAQTVIVGTGTDFPNIAFLNEKGELTGYDI
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCHH
EVMKAIDKELPQYTFEFKTMDFSNLLTSLGNKKIDVIAHNMAKNKEREKRFLYHKVPYNY
HHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCEEEEEEHHHHHCHHHHHHEEEEECCCCC
SPMYITVKEDNHKIHTLKDLHGKTVIVGATSNAADYITKYNKTHGSPIHLKYAGQGSNDT
CCEEEEEECCCCEEEEECCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCC
ANQIETGRADATIATPFAVDFQNKTHAFRQKTVGDVLLDTEVYFMFNKGSQTLADDTDQA
HHHHHCCCCCCEEECCEEEECCCCHHHHHHHHHCCEEECCEEEEEECCCCCCCCCCHHHH
IKKLEKNGTLKKLSRKWLGADYSKSSFEK
HHHHHHCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377