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The map label for this gene is mdh

Identifier: 16079964

GI number: 16079964

Start: 2978734

End: 2979672

Strand: Reverse

Name: mdh

Synonym: BSU29120

Alternate gene names: 16079964

Gene position: 2979672-2978734 (Counterclockwise)

Preceding gene: 16079965

Following gene: 255767672

Centisome position: 70.68

GC content: 45.15

Gene sequence:

>939_bases
ATGGGAAATACTCGTAAAAAAGTTTCTGTTATCGGAGCAGGTTTTACCGGAGCTACAACTGCATTTTTAATCGCTCAAAA
AGAGCTGGCAGACGTTGTTCTTGTTGACATTCCGCAATTGGAGAACCCGACAAAGGGAAAAGCGCTTGATATGCTTGAAG
CAAGCCCGGTTCAAGGCTTTGACGCAAAAATTACGGGAACATCCAATTACGAGGATACAGCCGGCTCTGACATTGTTGTC
ATTACAGCCGGTATCGCAAGAAAACCTGGTATGAGCAGAGATGATCTGGTCTCTACAAACGAAAAGATTATGAGAAGCGT
TACGCAGGAAATCGTGAAATATTCTCCTGACTCTATTATTGTGGTGCTGACAAATCCTGTTGATGCAATGACATACGCGG
TGTACAAAGAATCAGGCTTCCCTAAAGAGCGTGTAATCGGCCAGTCAGGTGTGCTTGATACGGCAAGATTCAGAACATTT
GTGGCAGAGGAATTAAACCTGTCAGTGAAAGATGTGACTGGTTTCGTACTCGGCGGACACGGTGACGATATGGTTCCGCT
TGTGCGTTATTCTTATGCTGGCGGTATCCCGCTTGAAACTCTTATTCCGAAAGAACGGATTGACGCAATTGTGGAGCGCA
CTAGAAAAGGCGGAGGCGAAATCGTGAATCTTCTTGGAAACGGAAGCGCGTATTATGCGCCTGCGGCTTCTCTGACAGAA
ATGGTCGAAGCGATCTTGAAAGATCAGCGCCGCGTCCTTCCTACAATTGCTTATCTTGAAGGGGAATACGGCTATGAAGG
CATCTACCTTGGTGTTCCTACAATTGTAGGCGGCAACGGTCTTGAGCAAATCATTGAACTTGAACTGACAGACTATGAAA
GAGCGCAGCTGAATAAATCAGTTGAATCTGTCAAAAATGTCATGAAAGTATTATCCTAA

Upstream 100 bases:

>100_bases
GCGACTGAAGTGAAATGTTCAGAGTTCGGAGAAGAACTGATCAAAAACATGGACTAAGCAAGGAAAAAGCCTAAAACTAG
CCATAAAGGAGAAGAGAGAC

Downstream 100 bases:

>100_bases
TAAAAAGAGAGAAAGGCTTGCTTAATACAGCCTTTCTCTTTTTACTATAAATGAAAGCGCTATCATAAACGTCTTTATTT
CTTTTAAAAATGATGTAAAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MGNTRKKVSVIGAGFTGATTAFLIAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVV
ITAGIARKPGMSRDDLVSTNEKIMRSVTQEIVKYSPDSIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTF
VAEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
MVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKSVESVKNVMKVLS

Sequences:

>Translated_312_residues
MGNTRKKVSVIGAGFTGATTAFLIAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVV
ITAGIARKPGMSRDDLVSTNEKIMRSVTQEIVKYSPDSIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTF
VAEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
MVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKSVESVKNVMKVLS
>Mature_311_residues
GNTRKKVSVIGAGFTGATTAFLIAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVVI
TAGIARKPGMSRDDLVSTNEKIMRSVTQEIVKYSPDSIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFV
AEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTEM
VEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKSVESVKNVMKVLS

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=316, Percent_Identity=37.3417721518987, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI221136809, Length=316, Percent_Identity=37.3417721518987, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI5031857, Length=316, Percent_Identity=36.0759493670886, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI260099723, Length=316, Percent_Identity=36.0759493670886, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI9257228, Length=318, Percent_Identity=36.7924528301887, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI4504973, Length=318, Percent_Identity=36.7924528301887, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI291575128, Length=314, Percent_Identity=36.624203821656, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI4557032, Length=314, Percent_Identity=36.624203821656, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI15082234, Length=316, Percent_Identity=34.4936708860759, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI260099727, Length=219, Percent_Identity=37.8995433789954, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI260099725, Length=213, Percent_Identity=38.4976525821596, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI207028494, Length=198, Percent_Identity=35.3535353535354, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI21735621, Length=319, Percent_Identity=28.8401253918495, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI103472011, Length=312, Percent_Identity=25.3205128205128, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI103472015, Length=184, Percent_Identity=29.3478260869565, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1789632, Length=316, Percent_Identity=31.0126582278481, Blast_Score=98, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17535107, Length=298, Percent_Identity=31.5436241610738, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17554310, Length=321, Percent_Identity=30.8411214953271, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322765, Length=294, Percent_Identity=29.2517006802721, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=227, Percent_Identity=27.3127753303965, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136226, Length=311, Percent_Identity=30.5466237942122, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45550422, Length=302, Percent_Identity=29.8013245033113, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24647881, Length=322, Percent_Identity=30.1242236024845, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24663599, Length=308, Percent_Identity=30.5194805194805, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24663595, Length=268, Percent_Identity=29.4776119402985, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33644; Mature: 33513

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNTRKKVSVIGAGFTGATTAFLIAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGF
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DAKITGTSNYEDTAGSDIVVITAGIARKPGMSRDDLVSTNEKIMRSVTQEIVKYSPDSII
CEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCEE
VVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGH
EEEECCHHHHHEEEHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCEEEHEEEEEEECC
GDDMVPLVRYSYAGGIPLETLIPKERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
CCCCHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCHHHHHH
MVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKS
HHHHHHHHHHHHCCHHEEEECCCCCCEEEEECCEEECCCCHHHHHEECCCCCHHHHHHHH
VESVKNVMKVLS
HHHHHHHHHHHC
>Mature Secondary Structure 
GNTRKKVSVIGAGFTGATTAFLIAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGF
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DAKITGTSNYEDTAGSDIVVITAGIARKPGMSRDDLVSTNEKIMRSVTQEIVKYSPDSII
CEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCEE
VVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGH
EEEECCHHHHHEEEHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCEEEHEEEEEEECC
GDDMVPLVRYSYAGGIPLETLIPKERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
CCCCHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCHHHHHH
MVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKS
HHHHHHHHHHHHCCHHEEEECCCCCCEEEEECCEEECCCCHHHHHEECCCCCHHHHHHHH
VESVKNVMKVLS
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA