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The map label for this gene is gapB

Identifier: 16079954

GI number: 16079954

Start: 2967032

End: 2968054

Strand: Reverse

Name: gapB

Synonym: BSU29020

Alternate gene names: 16079954

Gene position: 2968054-2967032 (Counterclockwise)

Preceding gene: 16079955

Following gene: 255767669

Centisome position: 70.41

GC content: 45.75

Gene sequence:

>1023_bases
ATGAAGGTAAAAGTAGCGATCAACGGGTTTGGAAGAATCGGAAGAATGGTTTTTAGAAAAGCGATGTTAGACGATCAAAT
TCAAGTAGTGGCCATTAACGCCAGCTATTCCGCAGAAACGCTGGCTCATTTAATAAAGTATGACACAATTCACGGCAGAT
ACGACAAAGAGGTTGTGGCTGGTGAAGATAGCCTGATCGTAAATGGAAAGAAAGTGCTTTTGTTAAACAGCCGTGATCCA
AAACAGCTGCCTTGGCGGGAATATGATATTGACATAGTCGTCGAAGCAACAGGGAAGTTTAATGCTAAAGATAAAGCGAT
GGGCCATATAGAAGCAGGTGCAAAAAAAGTGATTTTGACCGCTCCGGGAAAAAATGAAGACGTTACCATTGTGATGGGCG
TAAATGAGGACCAATTCGACGCTGAGCGCCATGTCATTATTTCAAATGCGTCATGCACGACAAATTGCCTTGCGCCTGTT
GTAAAAGTGCTGGATGAAGAGTTTGGCATTGAGAGCGGTCTGATGACTACAGTTCATGCGTATACGAATGACCAAAAAAA
TATTGATAACCCGCACAAAGATTTGCGCCGGGCGCGGGCTTGCGGTGAATCCATCATTCCAACAACAACAGGAGCGGCAA
AGGCGCTTTCGCTTGTGCTGCCGCATCTGAAAGGAAAACTTCACGGCCTCGCCTTGCGTGTCCCTGTTCCGAACGTCTCA
TTGGTTGATCTCGTTGTTGATCTGAAAACGGATGTTACGGCTGAAGAAGTAAACGAGGCATTTAAACGCGCTGCCAAAAC
GTCGATGTACGGTGTACTTGATTACTCAGATGAACCGCTCGTTTCGACTGATTATAATACGAATCCGCATTCAGCGGTCA
TTGACGGGCTTACAACAATGGTAATGGAAGACAGGAAAGTAAAGGTGCTGGCGTGGTATGACAACGAATGGGGCTACTCC
TGCAGAGTTGTTGATCTAATCCGCCATGTAGCGGCACGAATGAAACATCCGTCTGCTGTATAA

Upstream 100 bases:

>100_bases
TGATTTTTATCAATATGTACTGGCGAATTTGTTTTAATGTGTTATACTAATTTTAGATAGTAACAAATTAGGATGGCATA
ATTGATAAGGGGTGTCCAAC

Downstream 100 bases:

>100_bases
AATAAGGTCATGGACACATTTTAAAGAAAAAACCCTTAACAGCATATTTCTGAAAAAACGCACCTTGCAAATTAGACTTA
AACACAGTATACTATTTTTC

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase; GAPDH

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKVKVAINGFGRIGRMVFRKAMLDDQIQVVAINASYSAETLAHLIKYDTIHGRYDKEVVAGEDSLIVNGKKVLLLNSRDP
KQLPWREYDIDIVVEATGKFNAKDKAMGHIEAGAKKVILTAPGKNEDVTIVMGVNEDQFDAERHVIISNASCTTNCLAPV
VKVLDEEFGIESGLMTTVHAYTNDQKNIDNPHKDLRRARACGESIIPTTTGAAKALSLVLPHLKGKLHGLALRVPVPNVS
LVDLVVDLKTDVTAEEVNEAFKRAAKTSMYGVLDYSDEPLVSTDYNTNPHSAVIDGLTTMVMEDRKVKVLAWYDNEWGYS
CRVVDLIRHVAARMKHPSAV

Sequences:

>Translated_340_residues
MKVKVAINGFGRIGRMVFRKAMLDDQIQVVAINASYSAETLAHLIKYDTIHGRYDKEVVAGEDSLIVNGKKVLLLNSRDP
KQLPWREYDIDIVVEATGKFNAKDKAMGHIEAGAKKVILTAPGKNEDVTIVMGVNEDQFDAERHVIISNASCTTNCLAPV
VKVLDEEFGIESGLMTTVHAYTNDQKNIDNPHKDLRRARACGESIIPTTTGAAKALSLVLPHLKGKLHGLALRVPVPNVS
LVDLVVDLKTDVTAEEVNEAFKRAAKTSMYGVLDYSDEPLVSTDYNTNPHSAVIDGLTTMVMEDRKVKVLAWYDNEWGYS
CRVVDLIRHVAARMKHPSAV
>Mature_340_residues
MKVKVAINGFGRIGRMVFRKAMLDDQIQVVAINASYSAETLAHLIKYDTIHGRYDKEVVAGEDSLIVNGKKVLLLNSRDP
KQLPWREYDIDIVVEATGKFNAKDKAMGHIEAGAKKVILTAPGKNEDVTIVMGVNEDQFDAERHVIISNASCTTNCLAPV
VKVLDEEFGIESGLMTTVHAYTNDQKNIDNPHKDLRRARACGESIIPTTTGAAKALSLVLPHLKGKLHGLALRVPVPNVS
LVDLVVDLKTDVTAEEVNEAFKRAAKTSMYGVLDYSDEPLVSTDYNTNPHSAVIDGLTTMVMEDRKVKVLAWYDNEWGYS
CRVVDLIRHVAARMKHPSAV

Specific function: More active in anabolism

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7657116, Length=336, Percent_Identity=45.5357142857143, Blast_Score=305, Evalue=4e-83,
Organism=Homo sapiens, GI7669492, Length=334, Percent_Identity=43.7125748502994, Blast_Score=298, Evalue=4e-81,
Organism=Escherichia coli, GI1788079, Length=332, Percent_Identity=50.3012048192771, Blast_Score=334, Evalue=5e-93,
Organism=Escherichia coli, GI1789295, Length=330, Percent_Identity=47.2727272727273, Blast_Score=315, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17534677, Length=339, Percent_Identity=46.9026548672566, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17534679, Length=339, Percent_Identity=47.1976401179941, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI32566163, Length=339, Percent_Identity=45.7227138643068, Blast_Score=300, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI17568413, Length=339, Percent_Identity=45.7227138643068, Blast_Score=300, Evalue=7e-82,
Organism=Saccharomyces cerevisiae, GI6321631, Length=331, Percent_Identity=48.6404833836858, Blast_Score=326, Evalue=3e-90,
Organism=Saccharomyces cerevisiae, GI6322468, Length=331, Percent_Identity=48.6404833836858, Blast_Score=325, Evalue=9e-90,
Organism=Saccharomyces cerevisiae, GI6322409, Length=331, Percent_Identity=47.4320241691843, Blast_Score=319, Evalue=4e-88,
Organism=Drosophila melanogaster, GI85725000, Length=332, Percent_Identity=46.0843373493976, Blast_Score=302, Evalue=2e-82,
Organism=Drosophila melanogaster, GI22023983, Length=332, Percent_Identity=46.0843373493976, Blast_Score=302, Evalue=2e-82,
Organism=Drosophila melanogaster, GI17933600, Length=332, Percent_Identity=45.4819277108434, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI18110149, Length=332, Percent_Identity=45.4819277108434, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI19922412, Length=327, Percent_Identity=44.6483180428135, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): G3P2_BACSU (O34425)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   G69628
- RefSeq:   NP_390780.1
- ProteinModelPortal:   O34425
- SMR:   O34425
- EnsemblBacteria:   EBBACT00000003410
- GeneID:   937393
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29020
- NMPDR:   fig|224308.1.peg.2905
- GenoList:   BSU29020
- GeneTree:   EBGT00050000001163
- HOGENOM:   HBG571736
- OMA:   DFNTNPH
- PhylomeDB:   O34425
- ProtClustDB:   PRK07729
- BioCyc:   BSUB:BSU29020-MONOMER
- BRENDA:   1.2.1.59
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.59

Molecular weight: Translated: 37477; Mature: 37477

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00071 GAPDH

Important sites: ACT_SITE 152-152 BINDING 78-78 BINDING 182-182 BINDING 197-197 BINDING 233-233 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKVAINGFGRIGRMVFRKAMLDDQIQVVAINASYSAETLAHLIKYDTIHGRYDKEVVA
CEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEC
GEDSLIVNGKKVLLLNSRDPKQLPWREYDIDIVVEATGKFNAKDKAMGHIEAGAKKVILT
CCCCEEECCCEEEEEECCCCCCCCCEECCEEEEEECCCCCCCCHHHCEEECCCCEEEEEE
APGKNEDVTIVMGVNEDQFDAERHVIISNASCTTNCLAPVVKVLDEEFGIESGLMTTVHA
CCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEE
YTNDQKNIDNPHKDLRRARACGESIIPTTTGAAKALSLVLPHLKGKLHGLALRVPVPNVS
ECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCE
LVDLVVDLKTDVTAEEVNEAFKRAAKTSMYGVLDYSDEPLVSTDYNTNPHSAVIDGLTTM
EEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCEECCCCCCCHHHHHHHHHHH
VMEDRKVKVLAWYDNEWGYSCRVVDLIRHVAARMKHPSAV
HCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVKVAINGFGRIGRMVFRKAMLDDQIQVVAINASYSAETLAHLIKYDTIHGRYDKEVVA
CEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEC
GEDSLIVNGKKVLLLNSRDPKQLPWREYDIDIVVEATGKFNAKDKAMGHIEAGAKKVILT
CCCCEEECCCEEEEEECCCCCCCCCEECCEEEEEECCCCCCCCHHHCEEECCCCEEEEEE
APGKNEDVTIVMGVNEDQFDAERHVIISNASCTTNCLAPVVKVLDEEFGIESGLMTTVHA
CCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEE
YTNDQKNIDNPHKDLRRARACGESIIPTTTGAAKALSLVLPHLKGKLHGLALRVPVPNVS
ECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCE
LVDLVVDLKTDVTAEEVNEAFKRAAKTSMYGVLDYSDEPLVSTDYNTNPHSAVIDGLTTM
EEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCEECCCCCCCHHHHHHHHHHH
VMEDRKVKVLAWYDNEWGYSCRVVDLIRHVAARMKHPSAV
HCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10799476