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The map label for this gene is ysgA

Identifier: 16079917

GI number: 16079917

Start: 2930827

End: 2931573

Strand: Reverse

Name: ysgA

Synonym: BSU28650

Alternate gene names: 16079917

Gene position: 2931573-2930827 (Counterclockwise)

Preceding gene: 16079922

Following gene: 16079916

Centisome position: 69.54

GC content: 48.46

Gene sequence:

>747_bases
GTGAAACAAATAGAATCGGCAAAAAACCAAAAAGTGAAGGATTGGAAAAAGCTTCACACAAAAAAAGAACGAACAAAAAC
AAATACATTTCTGATAGAGGGAGAACACCTCGTTGAAGAGGCGTTGAAAAGCCCGGGCATTGTGAAAGAAATCCTTGTAA
AGGATGAAACGAGGATTCCTTCAGATCTTGAGACTGGTATCCAGTGCTATATGCTGAGTGAAGATGCGTTTTCAGCTGTG
ACTGAAACCGAAACGCCGCAGCAGATTGCCGCTGTGTGCCATATGCCTGAAGAAAAGCTGGCAACGGCACGCAAGGTGCT
GCTGATTGACGCTGTTCAAGATCCGGGAAACCTTGGCACAATGATTCGAACGGCTGATGCGGCCGGTTTGGATGCGGTTG
TACTCGGTGACGGCACGGCGGACGCCTTTAACGGAAAAACACTGCGATCTGCTCAAGGATCTCACTTCCATATTCCTGTT
GTGAGAAGAAACCTGCCTTCATATGTGGACGAATTGAAGGCAGAGGGCGTAAAAGTATACGGCACGGCGCTGCAAAACGG
AGCGCCTTACCAGGAGATTCCTCAGTCTGAATCCTTTGCGCTCATTGTAGGAAATGAAGGTGCAGGGGTAGATGCTGCAT
TGCTGGAAAAAACGGATTTGAACCTGTACGTGCCGCTTTACGGACAGGCTGAATCACTAAACGTTGCGGTTGCGGCTGCC
ATCCTCGTGTATCACTTGCGAGGATAG

Upstream 100 bases:

>100_bases
TAAAATGTAATACGTATATTCTAACCCTGCACCTGGTTTATTATGTGCTGTTCTTGCGATATAATAACATCATGTTAAAA
AAAGGAGCGCCACATAGACA

Downstream 100 bases:

>100_bases
TGTTGCGCCAAGCGTTGGATTTCTCTATAATAGAACATAATATTTCAAAAACCGTGACAGAGGACCTCAATTTCTGATCA
ACTGCTTTCCAGGGAGAAAA

Product: RNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKQIESAKNQKVKDWKKLHTKKERTKTNTFLIEGEHLVEEALKSPGIVKEILVKDETRIPSDLETGIQCYMLSEDAFSAV
TETETPQQIAAVCHMPEEKLATARKVLLIDAVQDPGNLGTMIRTADAAGLDAVVLGDGTADAFNGKTLRSAQGSHFHIPV
VRRNLPSYVDELKAEGVKVYGTALQNGAPYQEIPQSESFALIVGNEGAGVDAALLEKTDLNLYVPLYGQAESLNVAVAAA
ILVYHLRG

Sequences:

>Translated_248_residues
MKQIESAKNQKVKDWKKLHTKKERTKTNTFLIEGEHLVEEALKSPGIVKEILVKDETRIPSDLETGIQCYMLSEDAFSAV
TETETPQQIAAVCHMPEEKLATARKVLLIDAVQDPGNLGTMIRTADAAGLDAVVLGDGTADAFNGKTLRSAQGSHFHIPV
VRRNLPSYVDELKAEGVKVYGTALQNGAPYQEIPQSESFALIVGNEGAGVDAALLEKTDLNLYVPLYGQAESLNVAVAAA
ILVYHLRG
>Mature_248_residues
MKQIESAKNQKVKDWKKLHTKKERTKTNTFLIEGEHLVEEALKSPGIVKEILVKDETRIPSDLETGIQCYMLSEDAFSAV
TETETPQQIAAVCHMPEEKLATARKVLLIDAVQDPGNLGTMIRTADAAGLDAVVLGDGTADAFNGKTLRSAQGSHFHIPV
VRRNLPSYVDELKAEGVKVYGTALQNGAPYQEIPQSESFALIVGNEGAGVDAALLEKTDLNLYVPLYGQAESLNVAVAAA
ILVYHLRG

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family

Homologues:

Organism=Homo sapiens, GI8922534, Length=298, Percent_Identity=24.8322147651007, Blast_Score=81, Evalue=7e-16,
Organism=Escherichia coli, GI1790623, Length=140, Percent_Identity=32.1428571428571, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790083, Length=169, Percent_Identity=28.4023668639053, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=224, Percent_Identity=28.125, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24666840, Length=276, Percent_Identity=26.4492753623188, Blast_Score=80, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YSGA_BACSU (P94538)

Other databases:

- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   G69984
- RefSeq:   NP_390743.1
- HSSP:   P63177
- ProteinModelPortal:   P94538
- SMR:   P94538
- EnsemblBacteria:   EBBACT00000003769
- GeneID:   937444
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28650
- NMPDR:   fig|224308.1.peg.2868
- GenoList:   BSU28650
- GeneTree:   EBGT00050000002342
- HOGENOM:   HBG714057
- OMA:   KKERTKT
- ProtClustDB:   CLSK887695
- BioCyc:   BSUB:BSU28650-MONOMER
- InterPro:   IPR001537
- InterPro:   IPR013123
- SMART:   SM00967

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26896; Mature: 26896

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: BINDING 204-204 BINDING 224-224 BINDING 233-233

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIESAKNQKVKDWKKLHTKKERTKTNTFLIEGEHLVEEALKSPGIVKEILVKDETRIP
CCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCHHHHHHHCCCCCCC
SDLETGIQCYMLSEDAFSAVTETETPQQIAAVCHMPEEKLATARKVLLIDAVQDPGNLGT
CHHHCCEEEEEECCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHEEEEEECCCCCCCCH
MIRTADAAGLDAVVLGDGTADAFNGKTLRSAQGSHFHIPVVRRNLPSYVDELKAEGVKVY
HEEECCCCCCCEEEEECCCCCCCCCCEEECCCCCEEEEHHHHHCCHHHHHHHHHCCEEEE
GTALQNGAPYQEIPQSESFALIVGNEGAGVDAALLEKTDLNLYVPLYGQAESLNVAVAAA
EEECCCCCCHHHCCCCCCEEEEECCCCCCCCHHHEEECCCEEEEEECCCCCCCHHHHHHH
ILVYHLRG
HHHHHCCC
>Mature Secondary Structure
MKQIESAKNQKVKDWKKLHTKKERTKTNTFLIEGEHLVEEALKSPGIVKEILVKDETRIP
CCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCHHHHHHHCCCCCCC
SDLETGIQCYMLSEDAFSAVTETETPQQIAAVCHMPEEKLATARKVLLIDAVQDPGNLGT
CHHHCCEEEEEECCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHEEEEEECCCCCCCCH
MIRTADAAGLDAVVLGDGTADAFNGKTLRSAQGSHFHIPVVRRNLPSYVDELKAEGVKVY
HEEECCCCCCCEEEEECCCCCCCCCCEEECCCCCEEEEHHHHHCCHHHHHHHHHCCEEEE
GTALQNGAPYQEIPQSESFALIVGNEGAGVDAALLEKTDLNLYVPLYGQAESLNVAVAAA
EEECCCCCCHHHCCCCCCEEEEECCCCCCCCHHHEEECCCEEEEEECCCCCCCHHHHHHH
ILVYHLRG
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377