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The map label for this gene is pheS

Identifier: 16079916

GI number: 16079916

Start: 2929438

End: 2930472

Strand: Reverse

Name: pheS

Synonym: BSU28640

Alternate gene names: 16079916

Gene position: 2930472-2929438 (Counterclockwise)

Preceding gene: 16079917

Following gene: 16079915

Centisome position: 69.51

GC content: 46.76

Gene sequence:

>1035_bases
ATGGAAGAAAAGCTAAAACAGCTGGAACAAGAAGCTTTAGAACAAGTAGAAGCGGCAAGCTCATTGAAGGTTGTCAATGA
TATTCGGGTGCAATATCTCGGAAAAAAAGGGCCGATTACAGAAGTGCTGCGCGGAATGGGCAAGCTTTCTGCTGAGGAAC
GTCCAAAAATGGGGGCGCTCGCGAACGAAGTAAGGGAGCGTATTGCCAATGCGATTGCTGACAAAAACGAGAAGCTTGAA
GAAGAGGAAATGAAACAGAAGCTTGCAGGACAGACAATTGACGTCACGCTGCCGGGGAACCCTGTTGCAGTCGGCGGCCG
CCATCCGCTCACTGTTGTCATTGAAGAAATTGAAGATTTATTTATCGGTATGGGCTACACAGTCGAGGAAGGGCCAGAGG
TTGAAACGGATTACTACAACTTCGAATCGCTCAATCTTCCGAAAGAACACCCAGCGCGCGATATGCAGGACAGCTTTTAC
ATCACAGAGGAAACTTTGATGAGAACGCAAACTTCTCCTGTCCAAACACGTACGATGGAAAAGCATGAAGGCAAAGGTCC
CGTTAAAATCATTTGCCCGGGTAAAGTATATCGCCGTGATAACGATGATGCGACGCACTCTCACCAATTTATGCAAATTG
AAGGGCTTGTCGTTGACAAAAACATCAGCATGAGTGATTTAAAAGGAACGCTTGAACTTGTTGCGAAAAAAATGTTCGGG
CAAGACCGTGAAATCAGACTCCGCCCAAGCTTCTTCCCGTTTACTGAGCCTTCAGTAGAAGTGGATGTGACATGCTTTAA
ATGCGGTGGGAACGGCTGCTCAGTATGTAAAGGAACAGGCTGGATTGAAATCCTCGGTGCCGGAATGGTTCACCCGAACG
TGCTTAAAATGGCTGGCTTTGATCCGAAGGAATATCAGGGCTTCGCATTCGGAATGGGTGTTGAGCGCATCGCGATGCTG
AAATATGGCATTGATGATATCCGCCACTTCTATACAAACGATGTCAGATTTATTTCGCAGTTTAAACAGGCGTAA

Upstream 100 bases:

>100_bases
GGTGGTACCGCGGCCACAACTCGTCCCTTGTACAAGGGACGGGTTTTTTTTATTTTCCAAAGACATATGACGGTTTGTTT
ACATAAAAGGAGGATGCAAA

Downstream 100 bases:

>100_bases
GAGGAGGATGCAAGGATGTTTGTTTCTTATAAATGGTTAGAGGATTATGTTGATTTAAAAGGCATGGACCCGGCTGTTCT
TGCTGAAAAAATTACAAGAG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MEEKLKQLEQEALEQVEAASSLKVVNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGALANEVRERIANAIADKNEKLE
EEEMKQKLAGQTIDVTLPGNPVAVGGRHPLTVVIEEIEDLFIGMGYTVEEGPEVETDYYNFESLNLPKEHPARDMQDSFY
ITEETLMRTQTSPVQTRTMEKHEGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLVVDKNISMSDLKGTLELVAKKMFG
QDREIRLRPSFFPFTEPSVEVDVTCFKCGGNGCSVCKGTGWIEILGAGMVHPNVLKMAGFDPKEYQGFAFGMGVERIAML
KYGIDDIRHFYTNDVRFISQFKQA

Sequences:

>Translated_344_residues
MEEKLKQLEQEALEQVEAASSLKVVNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGALANEVRERIANAIADKNEKLE
EEEMKQKLAGQTIDVTLPGNPVAVGGRHPLTVVIEEIEDLFIGMGYTVEEGPEVETDYYNFESLNLPKEHPARDMQDSFY
ITEETLMRTQTSPVQTRTMEKHEGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLVVDKNISMSDLKGTLELVAKKMFG
QDREIRLRPSFFPFTEPSVEVDVTCFKCGGNGCSVCKGTGWIEILGAGMVHPNVLKMAGFDPKEYQGFAFGMGVERIAML
KYGIDDIRHFYTNDVRFISQFKQA
>Mature_344_residues
MEEKLKQLEQEALEQVEAASSLKVVNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGALANEVRERIANAIADKNEKLE
EEEMKQKLAGQTIDVTLPGNPVAVGGRHPLTVVIEEIEDLFIGMGYTVEEGPEVETDYYNFESLNLPKEHPARDMQDSFY
ITEETLMRTQTSPVQTRTMEKHEGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLVVDKNISMSDLKGTLELVAKKMFG
QDREIRLRPSFFPFTEPSVEVDVTCFKCGGNGCSVCKGTGWIEILGAGMVHPNVLKMAGFDPKEYQGFAFGMGVERIAML
KYGIDDIRHFYTNDVRFISQFKQA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=278, Percent_Identity=29.136690647482, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI5729820, Length=278, Percent_Identity=29.4964028776978, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=48.2248520710059, Blast_Score=337, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=28.1021897810219, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=28.1021897810219, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=254, Percent_Identity=34.251968503937, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=367, Percent_Identity=26.158038147139, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=345, Percent_Identity=27.2463768115942, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI18858079, Length=274, Percent_Identity=29.1970802919708, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137424, Length=294, Percent_Identity=27.5510204081633, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_BACSU (P17921)

Other databases:

- EMBL:   X53057
- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   H69675
- RefSeq:   NP_390742.1
- ProteinModelPortal:   P17921
- SMR:   P17921
- EnsemblBacteria:   EBBACT00000002740
- GeneID:   937987
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28640
- NMPDR:   fig|224308.1.peg.2867
- GenoList:   BSU28640
- GeneTree:   EBGT00050000002461
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   P17921
- ProtClustDB:   PRK00488
- BioCyc:   BSUB:BSU28640-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38676; Mature: 38676

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKLKQLEQEALEQVEAASSLKVVNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHH
ANEVRERIANAIADKNEKLEEEEMKQKLAGQTIDVTLPGNPVAVGGRHPLTVVIEEIEDL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHHHHH
FIGMGYTVEEGPEVETDYYNFESLNLPKEHPARDMQDSFYITEETLMRTQTSPVQTRTME
HHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHCCCCCCHHHHHH
KHEGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLVVDKNISMSDLKGTLELVAKKMFG
HHCCCCCEEEEECCHHEECCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHC
QDREIRLRPSFFPFTEPSVEVDVTCFKCGGNGCSVCKGTGWIEILGAGMVHPNVLKMAGF
CCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHCCC
DPKEYQGFAFGMGVERIAMLKYGIDDIRHFYTNDVRFISQFKQA
CHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEEKLKQLEQEALEQVEAASSLKVVNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHH
ANEVRERIANAIADKNEKLEEEEMKQKLAGQTIDVTLPGNPVAVGGRHPLTVVIEEIEDL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHHHHH
FIGMGYTVEEGPEVETDYYNFESLNLPKEHPARDMQDSFYITEETLMRTQTSPVQTRTME
HHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHCCCCCCHHHHHH
KHEGKGPVKIICPGKVYRRDNDDATHSHQFMQIEGLVVDKNISMSDLKGTLELVAKKMFG
HHCCCCCEEEEECCHHEECCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHC
QDREIRLRPSFFPFTEPSVEVDVTCFKCGGNGCSVCKGTGWIEILGAGMVHPNVLKMAGF
CCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHCCC
DPKEYQGFAFGMGVERIAMLKYGIDDIRHFYTNDVRFISQFKQA
CHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2127701; 1903307; 8969504; 9384377