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The map label for this gene is yshC

Identifier: 16079911

GI number: 16079911

Start: 2923314

End: 2925026

Strand: Reverse

Name: yshC

Synonym: BSU28590

Alternate gene names: 16079911

Gene position: 2925026-2923314 (Counterclockwise)

Preceding gene: 16079912

Following gene: 16079910

Centisome position: 69.39

GC content: 48.1

Gene sequence:

>1713_bases
ATGCATAAAAAAGATATTATCCGGCTGCTTGAAACCATTGCAGTCTATATGGAACTCAAAGGGGACAACCCGTTTAAAGT
ATCCGCCTTCCGAAAAGCGGCGGCAGCTCTGGAACAGGACGACCGAAGCTTATCAGAAATGGACGATATGATGTCGCTGT
CCGGCATTGGAAAAGGAACGTACAGCGTCATTAAAGAGTATATAGATGAAGGAAAGTCAAGCACGCTCGAATCGCTTCAG
AAAGAAGTGCCGGAAGGACTCGTGCCTTTATTGAAGCTGCCGGGGCTCGGCGGCAAAAAAATCGCAAAGCTGTATAAAGA
GCTCGGCGTACACGATGCAGAATCTCTGAAGGAAGCCTGTGAGCAGCAAAAGGTCCAGGGTCTTGCGGGCTTCGGCAAAA
AATCGGAAGAAAAAATATTACAGGCGCTCGGGGAAGCCGGAAAACAGCCTGAACGGTTCCCGATCGGCTACGCCCTCCGG
ATCGCGCGGGAAATTGAGGAGCATCTTTCTCAATTTACGCATATCATCAAATTTTCTCGTGCCGGAAGTCTCCGCAGAGC
GCGGGAAACGGTGAAGGATCTGGATTATATCATTGCTACAGATCATCCGGCTGAAGTAAGAGAGCAGCTTCTTGAGCTGC
CAAACATCAAAAGCGTGATCGCAAGCGGAGATACGAAGGTGTCCGTCATCCTTTCCTTTGAATATGAAACAAGCGTCGAT
TTCCGTCTTGTGACGGAAGAGCAGTTTCCGACAACACTCCATCATTTTACGGGATCAAAGGATCACAATATTAAGATGCG
CCAAATCGCCAAAGAACGCGGCGAGCGGATCAGTGAATACGGCGTTGAGACAGTTGAAACCGGAGAGATCAAAACATTTC
CGAGTGAACGTGAATTTTATGCGCACTTCGGCCTGCCGCTGATTCCGCCGGAAATTCGTGAAAGCGGACAGGAAGTGGAA
ACCTACAGCGACAGCATTGAACTGATAGAGCTCGGGCAAATCAAAGGTGATCTCCACATGCACTCAACGTGGAGCGATGG
CGCATTTTCGATCAGAGAGATGGCTGAAGCCTGCATCAAAAAAGGCTATCAATACATGGCGATCACCGATCACTCACAAT
ATTTAAAGGTCGCCAACGGGCTTACTGCAGAAAGACTTAAGCAGCAGGCAAAAGAAATTGACGCGCTGAATGCAGAGTTT
GAAAACTTCCGTATTTTGAAAGGCGTTGAGATGGATATCCTGCCTGACGGTACGCTCGATTATGATGACGATGTGCTTGC
GGAAATGGATATCGTTATTGCATCGATTCATTCCAGTTTTAATCAGCCGGAACACGTGATTATGAAACGGCTTGAGACGG
CACTGACAAACAAGCATGTTGACATTATCGCACACCCGACCGGGCGCCTTATCGGCAGACGGGCCGGTTACGAAATCGAT
ATTGATCAGCTGATTGAGCTTGCGAGGAAAACAAATACGGCGCTTGAACTCAATGCCAACCCTGCGCGCCTTGATCTGCG
CACAGAGCATTTAATGAAAGCAAACGAACAAGGGGTTACATTGGTGATTAATACTGATGCCCATAATATTGAGATGTTAG
ACGATATGAAAACTGGCGTTACTGCCGCGCGCAAAGGATGGACGGAAACGAAAAACGTTCTGAATGCCCGGTCGCTTAAA
GACGTAGAGGCATTTCTGAAGCGCAACGATTAA

Upstream 100 bases:

>100_bases
GAGCTGTGGACACAGTACGGGGCATAAAAAAACTTCTCTTGTCCGGAGAAGTTTTTTTCAAGTATGATGGAAACATATGT
GAACGTCTGGGGGTTATGAT

Downstream 100 bases:

>100_bases
GTAAGGAGGCTCACACAATCGTGCAGCAAAAAGTATTATCAGCTCTTGAATTTCATAAAGTGAAAGAACAGGTTATTGGG
CATGCCGCTTCATCGCTCGG

Product: hypothetical protein

Products: NA

Alternate protein names: DNA polymerase type-X; 3'-5' exodeoxyribonuclease; 3'-5' exonuclease

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MHKKDIIRLLETIAVYMELKGDNPFKVSAFRKAAAALEQDDRSLSEMDDMMSLSGIGKGTYSVIKEYIDEGKSSTLESLQ
KEVPEGLVPLLKLPGLGGKKIAKLYKELGVHDAESLKEACEQQKVQGLAGFGKKSEEKILQALGEAGKQPERFPIGYALR
IAREIEEHLSQFTHIIKFSRAGSLRRARETVKDLDYIIATDHPAEVREQLLELPNIKSVIASGDTKVSVILSFEYETSVD
FRLVTEEQFPTTLHHFTGSKDHNIKMRQIAKERGERISEYGVETVETGEIKTFPSEREFYAHFGLPLIPPEIRESGQEVE
TYSDSIELIELGQIKGDLHMHSTWSDGAFSIREMAEACIKKGYQYMAITDHSQYLKVANGLTAERLKQQAKEIDALNAEF
ENFRILKGVEMDILPDGTLDYDDDVLAEMDIVIASIHSSFNQPEHVIMKRLETALTNKHVDIIAHPTGRLIGRRAGYEID
IDQLIELARKTNTALELNANPARLDLRTEHLMKANEQGVTLVINTDAHNIEMLDDMKTGVTAARKGWTETKNVLNARSLK
DVEAFLKRND

Sequences:

>Translated_570_residues
MHKKDIIRLLETIAVYMELKGDNPFKVSAFRKAAAALEQDDRSLSEMDDMMSLSGIGKGTYSVIKEYIDEGKSSTLESLQ
KEVPEGLVPLLKLPGLGGKKIAKLYKELGVHDAESLKEACEQQKVQGLAGFGKKSEEKILQALGEAGKQPERFPIGYALR
IAREIEEHLSQFTHIIKFSRAGSLRRARETVKDLDYIIATDHPAEVREQLLELPNIKSVIASGDTKVSVILSFEYETSVD
FRLVTEEQFPTTLHHFTGSKDHNIKMRQIAKERGERISEYGVETVETGEIKTFPSEREFYAHFGLPLIPPEIRESGQEVE
TYSDSIELIELGQIKGDLHMHSTWSDGAFSIREMAEACIKKGYQYMAITDHSQYLKVANGLTAERLKQQAKEIDALNAEF
ENFRILKGVEMDILPDGTLDYDDDVLAEMDIVIASIHSSFNQPEHVIMKRLETALTNKHVDIIAHPTGRLIGRRAGYEID
IDQLIELARKTNTALELNANPARLDLRTEHLMKANEQGVTLVINTDAHNIEMLDDMKTGVTAARKGWTETKNVLNARSLK
DVEAFLKRND
>Mature_570_residues
MHKKDIIRLLETIAVYMELKGDNPFKVSAFRKAAAALEQDDRSLSEMDDMMSLSGIGKGTYSVIKEYIDEGKSSTLESLQ
KEVPEGLVPLLKLPGLGGKKIAKLYKELGVHDAESLKEACEQQKVQGLAGFGKKSEEKILQALGEAGKQPERFPIGYALR
IAREIEEHLSQFTHIIKFSRAGSLRRARETVKDLDYIIATDHPAEVREQLLELPNIKSVIASGDTKVSVILSFEYETSVD
FRLVTEEQFPTTLHHFTGSKDHNIKMRQIAKERGERISEYGVETVETGEIKTFPSEREFYAHFGLPLIPPEIRESGQEVE
TYSDSIELIELGQIKGDLHMHSTWSDGAFSIREMAEACIKKGYQYMAITDHSQYLKVANGLTAERLKQQAKEIDALNAEF
ENFRILKGVEMDILPDGTLDYDDDVLAEMDIVIASIHSSFNQPEHVIMKRLETALTNKHVDIIAHPTGRLIGRRAGYEID
IDQLIELARKTNTALELNANPARLDLRTEHLMKANEQGVTLVINTDAHNIEMLDDMKTGVTAARKGWTETKNVLNARSLK
DVEAFLKRND

Specific function: Strictly DNA-template-directed DNA polymerase, preferentially acting on DNA structures containing gaps from one to a few nucleotides and bearing a phosphate group at the 5' end of the downstream DNA. The fact that PolX is able to conduct filling of a sing

COG id: COG1796

COG function: function code L; DNA polymerase IV (family X)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PHP family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): POLX_BACSU (P94544)

Other databases:

- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   C69985
- RefSeq:   NP_390737.1
- HSSP:   P75914
- ProteinModelPortal:   P94544
- EnsemblBacteria:   EBBACT00000000250
- GeneID:   937426
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28590
- NMPDR:   fig|224308.1.peg.2862
- GenoList:   BSU28590
- GeneTree:   EBGT00050000001534
- HOGENOM:   HBG450256
- OMA:   ARGYKFM
- ProtClustDB:   PRK08609
- BioCyc:   BSUB:BSU28590-MONOMER
- InterPro:   IPR002054
- InterPro:   IPR010996
- InterPro:   IPR003583
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR022311
- Gene3D:   G3DSA:1.10.150.110
- PIRSF:   PIRSF005047
- SMART:   SM00278
- SMART:   SM00481
- SMART:   SM00483

Pfam domain/function: PF02811 PHP; SSF47802 DNApol_B_N_like; SSF89550 PHP-like

EC number: =2.7.7.7

Molecular weight: Translated: 64121; Mature: 64121

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00522 DNA_POLYMERASE_X

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKKDIIRLLETIAVYMELKGDNPFKVSAFRKAAAALEQDDRSLSEMDDMMSLSGIGKGT
CCHHHHHHHHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
YSVIKEYIDEGKSSTLESLQKEVPEGLVPLLKLPGLGGKKIAKLYKELGVHDAESLKEAC
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHH
EQQKVQGLAGFGKKSEEKILQALGEAGKQPERFPIGYALRIAREIEEHLSQFTHIIKFSR
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
AGSLRRARETVKDLDYIIATDHPAEVREQLLELPNIKSVIASGDTKVSVILSFEYETSVD
CCHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEEEECCCCC
FRLVTEEQFPTTLHHFTGSKDHNIKMRQIAKERGERISEYGVETVETGEIKTFPSEREFY
EEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCEECCCCCCHHH
AHFGLPLIPPEIRESGQEVETYSDSIELIELGQIKGDLHMHSTWSDGAFSIREMAEACIK
HHCCCCCCCHHHHHCCCHHHHHCCCHHEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHH
KGYQYMAITDHSQYLKVANGLTAERLKQQAKEIDALNAEFENFRILKGVEMDILPDGTLD
CCCEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCEEEECCCEEEECCCCCCC
YDDDVLAEMDIVIASIHSSFNQPEHVIMKRLETALTNKHVDIIAHPTGRLIGRRAGYEID
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCEEC
IDQLIELARKTNTALELNANPARLDLRTEHLMKANEQGVTLVINTDAHNIEMLDDMKTGV
HHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
TAARKGWTETKNVLNARSLKDVEAFLKRND
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHKKDIIRLLETIAVYMELKGDNPFKVSAFRKAAAALEQDDRSLSEMDDMMSLSGIGKGT
CCHHHHHHHHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
YSVIKEYIDEGKSSTLESLQKEVPEGLVPLLKLPGLGGKKIAKLYKELGVHDAESLKEAC
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHH
EQQKVQGLAGFGKKSEEKILQALGEAGKQPERFPIGYALRIAREIEEHLSQFTHIIKFSR
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
AGSLRRARETVKDLDYIIATDHPAEVREQLLELPNIKSVIASGDTKVSVILSFEYETSVD
CCHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEEEECCCCC
FRLVTEEQFPTTLHHFTGSKDHNIKMRQIAKERGERISEYGVETVETGEIKTFPSEREFY
EEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCEECCCCCCHHH
AHFGLPLIPPEIRESGQEVETYSDSIELIELGQIKGDLHMHSTWSDGAFSIREMAEACIK
HHCCCCCCCHHHHHCCCHHHHHCCCHHEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHH
KGYQYMAITDHSQYLKVANGLTAERLKQQAKEIDALNAEFENFRILKGVEMDILPDGTLD
CCCEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCEEEECCCEEEECCCCCCC
YDDDVLAEMDIVIASIHSSFNQPEHVIMKRLETALTNKHVDIIAHPTGRLIGRRAGYEID
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCEEC
IDQLIELARKTNTALELNANPARLDLRTEHLMKANEQGVTLVINTDAHNIEMLDDMKTGV
HHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
TAARKGWTETKNVLNARSLKDVEAFLKRND
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377