BacMap

Definition	Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession	NC_000964
Length	4,215,606

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The map label for this gene is ysiB

Identifier: 16079906

GI number: 16079906

Start: 2917166

End: 2917942

Strand: Reverse

Name: ysiB

Synonym: BSU28540

Alternate gene names: 16079906

Gene position: 2917942-2917166 (Counterclockwise)

Preceding gene: 16079907

Following gene: 16079905

Centisome position: 69.22

GC content: 48.52

Gene sequence:

>777_bases
ATGAATGCAATTTCACTTGCGGTTGATCAATTTGTGGCAGTCTTGACGATTCACAATCCGCCGGCAAACGCGCTTTCAAG
CCGGATATTAGAAGAGCTGTCTTCCTGTCTTGATCAATGTGAGACAGATGCAGGCGTACGAAGCATTATCATTCACGGCG
AGGGAAGATTTTTCTCAGCCGGCGCTGATATTAAAGAATTTACATCGCTTAAAGGCAATGAGGATTCCTCATTGCTCGCT
GAGCGCGGCCAGCAGTTAATGGAGAGAATCGAAAGTTTTCCAAAACCGATTATCGCAGCGATTCACGGCGCTGCTCTCGG
AGGCGGGCTGGAGCTTGCAATGGCCTGTCACATCAGAATCGCGGCAGAAGATGCTAAGCTGGGGCTCCCGGAGCTTAATC
TCGGTATCATTCCAGGTTTTGCGGGGACACAGCGCCTTCCGAGATATGTCGGTACAGCCAAAGCACTGGAGCTCATCGGA
TCAGGAGAACCGATATCCGGAAAAGAAGCGCTTGATCTCGGACTTGTCTCAATTGGAGCGAAAGATGAGGCAGAAGTGAT
TGAAAAAGCAAAAGCACTTGCCGCTAAGTTTGCAGAGAAAAGCCCGCAAACGCTGGCATCTTTGCTTGAACTTCTTTATT
CCAACAAGGTGTATTCCTATGAAGGCAGCTTAAAGCTTGAAGCAAAGCGATTTGGGGAAGCATTTGAGTCAGAGGACGCC
AAGGAAGGCATCCAGGCATTTCTCGAAAAAAGAAAGCCTCAGTTCAAAGGCGAATAA

Upstream 100 bases:

>100_bases
GGGTGATGAATGACCAAAAGTACGATCTCGTTGCGCTTTCAAACAGCGTTTTAGAATTATTGGTATCCGGAATTCACAAT
AAGTAAAGGAGTTCTGTCCT

Downstream 100 bases:

>100_bases
AAGGGGATATGATCATGAATCTATTTGTACTGATGAAACGGACGTTTGACACAGAAGAAAAAATCGTCATTGAAACAGGA
AAGATTCAGGATGACGGAGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNAISLAVDQFVAVLTIHNPPANALSSRILEELSSCLDQCETDAGVRSIIIHGEGRFFSAGADIKEFTSLKGNEDSSLLA
ERGQQLMERIESFPKPIIAAIHGAALGGGLELAMACHIRIAAEDAKLGLPELNLGIIPGFAGTQRLPRYVGTAKALELIG
SGEPISGKEALDLGLVSIGAKDEAEVIEKAKALAAKFAEKSPQTLASLLELLYSNKVYSYEGSLKLEAKRFGEAFESEDA
KEGIQAFLEKRKPQFKGE

Sequences:

>Translated_258_residues
MNAISLAVDQFVAVLTIHNPPANALSSRILEELSSCLDQCETDAGVRSIIIHGEGRFFSAGADIKEFTSLKGNEDSSLLA
ERGQQLMERIESFPKPIIAAIHGAALGGGLELAMACHIRIAAEDAKLGLPELNLGIIPGFAGTQRLPRYVGTAKALELIG
SGEPISGKEALDLGLVSIGAKDEAEVIEKAKALAAKFAEKSPQTLASLLELLYSNKVYSYEGSLKLEAKRFGEAFESEDA
KEGIQAFLEKRKPQFKGE
>Mature_258_residues
MNAISLAVDQFVAVLTIHNPPANALSSRILEELSSCLDQCETDAGVRSIIIHGEGRFFSAGADIKEFTSLKGNEDSSLLA
ERGQQLMERIESFPKPIIAAIHGAALGGGLELAMACHIRIAAEDAKLGLPELNLGIIPGFAGTQRLPRYVGTAKALELIG
SGEPISGKEALDLGLVSIGAKDEAEVIEKAKALAAKFAEKSPQTLASLLELLYSNKVYSYEGSLKLEAKRFGEAFESEDA
KEGIQAFLEKRKPQFKGE

Specific function: Involved in the degradation of long-chain fatty acids

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=37.4501992031873, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI68989263, Length=165, Percent_Identity=41.8181818181818, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=39.3258426966292, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI4502327, Length=240, Percent_Identity=39.5833333333333, Blast_Score=121, Evalue=5e-28,
Organism=Homo sapiens, GI70995211, Length=242, Percent_Identity=32.2314049586777, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=31.8548387096774, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=27.3913043478261, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=27.3913043478261, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI157694520, Length=191, Percent_Identity=27.7486910994764, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI150378541, Length=255, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI261878539, Length=72, Percent_Identity=56.9444444444444, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI62530384, Length=246, Percent_Identity=26.0162601626016, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI37594469, Length=240, Percent_Identity=26.25, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI37594471, Length=220, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221307494, Length=168, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI221136756, Length=249, Percent_Identity=24.8995983935743, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221136753, Length=249, Percent_Identity=24.8995983935743, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI25453481, Length=167, Percent_Identity=25.748502994012, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI51538573, Length=167, Percent_Identity=25.748502994012, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4757966, Length=167, Percent_Identity=25.748502994012, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI51538581, Length=167, Percent_Identity=25.748502994012, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI116235487, Length=167, Percent_Identity=28.1437125748503, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=39.2307692307692, Blast_Score=170, Evalue=9e-44,
Organism=Escherichia coli, GI1788682, Length=170, Percent_Identity=44.1176470588235, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=34, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790281, Length=173, Percent_Identity=36.9942196531792, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=30.4182509505703, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=32.824427480916, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=29.2, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=39.5256916996047, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=37.5510204081633, Blast_Score=150, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17558304, Length=170, Percent_Identity=42.3529411764706, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17540714, Length=196, Percent_Identity=37.7551020408163, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17508951, Length=156, Percent_Identity=41.025641025641, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17508953, Length=156, Percent_Identity=41.025641025641, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25144276, Length=156, Percent_Identity=41.025641025641, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17534483, Length=262, Percent_Identity=30.9160305343511, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=254, Percent_Identity=29.5275590551181, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=230, Percent_Identity=28.695652173913, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=247, Percent_Identity=27.9352226720648, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=26.8867924528302, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=237, Percent_Identity=25.7383966244726, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=163, Percent_Identity=28.2208588957055, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=210, Percent_Identity=28.0952380952381, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=39.3700787401575, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=39.3700787401575, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=37.2549019607843, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921000, Length=172, Percent_Identity=40.6976744186046, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24583077, Length=172, Percent_Identity=40.6976744186046, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24583079, Length=172, Percent_Identity=40.6976744186046, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19920382, Length=260, Percent_Identity=30.7692307692308, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357171, Length=257, Percent_Identity=30.7392996108949, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=24.3027888446215, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583165, Length=195, Percent_Identity=29.2307692307692, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921018, Length=197, Percent_Identity=29.9492385786802, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24650670, Length=155, Percent_Identity=30.9677419354839, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550169, Length=195, Percent_Identity=27.6923076923077, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FADB_BACSU (P94549)

Other databases:

- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   G69985
- RefSeq:   NP_390732.1
- HSSP:   P14604
- ProteinModelPortal:   P94549
- SMR:   P94549
- EnsemblBacteria:   EBBACT00000000471
- GeneID:   937448
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28540
- NMPDR:   fig|224308.1.peg.2857
- GenoList:   BSU28540
- GeneTree:   EBGT00070000031862
- HOGENOM:   HBG748731
- OMA:   ACHIRIA
- PhylomeDB:   P94549
- ProtClustDB:   PRK07658
- BioCyc:   BSUB:BSU28540-MONOMER
- InterPro:   IPR001753
- InterPro:   IPR018376

Pfam domain/function: PF00378 ECH

EC number: =4.2.1.17

Molecular weight: Translated: 27554; Mature: 27554

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAISLAVDQFVAVLTIHNPPANALSSRILEELSSCLDQCETDAGVRSIIIHGEGRFFSA
CCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEC
GADIKEFTSLKGNEDSSLLAERGQQLMERIESFPKPIIAAIHGAALGGGLELAMACHIRI
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHEEEEE
AAEDAKLGLPELNLGIIPGFAGTQRLPRYVGTAKALELIGSGEPISGKEALDLGLVSIGA
EECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
KDEAEVIEKAKALAAKFAEKSPQTLASLLELLYSNKVYSYEGSLKLEAKRFGEAFESEDA
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCEEEHHHHHHHHHCCCHH
KEGIQAFLEKRKPQFKGE
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNAISLAVDQFVAVLTIHNPPANALSSRILEELSSCLDQCETDAGVRSIIIHGEGRFFSA
CCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEC
GADIKEFTSLKGNEDSSLLAERGQQLMERIESFPKPIIAAIHGAALGGGLELAMACHIRI
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHEEEEE
AAEDAKLGLPELNLGIIPGFAGTQRLPRYVGTAKALELIGSGEPISGKEALDLGLVSIGA
EECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
KDEAEVIEKAKALAAKFAEKSPQTLASLLELLYSNKVYSYEGSLKLEAKRFGEAFESEDA
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCEEEHHHHHHHHHCCCHH
KEGIQAFLEKRKPQFKGE
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377