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The map label for this gene is lonB

Identifier: 16079873

GI number: 16079873

Start: 2882971

End: 2884629

Strand: Reverse

Name: lonB

Synonym: BSU28210

Alternate gene names: 16079873

Gene position: 2884629-2882971 (Counterclockwise)

Preceding gene: 255767648

Following gene: 16079872

Centisome position: 68.43

GC content: 46.41

Gene sequence:

>1659_bases
ATGAGTTGGACAGGGATCGCGTTATTTATTCAGCTGTTTTTTGGGATCATCATCGGTTTGTATTTTTGGAATTTGCTTAA
AAATCAAAGGACACAAAAGGTCACGATAGATAAAGAATCAAAGAAGGAAATGGAGCAGCTTCGGAAAATGCGCGCTATCA
GCTTGTCCGAGCCATTATCTGAGAAGGTCCGCCCCAAAAGCTTTAAAGATATCGTCGGACAGGAAGACGGCATTAAAGCA
TTAAAGGCGGCTTTATGCGGGCCAAATCCTCAGCATGTGATTGTCTATGGGCCACCGGGAGTTGGGAAAACGGCTGCTGC
GCGGCTTGTGTTGGAAGAAGCAAAAAAACATAAACAATCACCGTTTAAAGAGCAGGCTGTCTTTGTGGAACTCGATGCGA
CAACAGCCCGTTTTGATGAAAGAGGAATTGCCGATCCGCTCATCGGCTCTGTCCATGACCCGATATACCAAGGGGCCGGA
GCGATGGGACAGGCTGGGATTCCGCAGCCGAAGCAGGGAGCTGTCACCCATGCCCATGGCGGTGTGCTGTTTATTGATGA
AATAGGGGAGCTGCATCCTATTCAAATGAATAAAATGCTGAAGGTGCTTGAGGACAGAAAAGTCTTTTTAGACAGTGCGT
ATTACAGTGAGGAAAATACACAAATTCCGAATCATATTCATGATATTTTTCAAAATGGGCTGCCGGCTGATTTTCGCTTG
ATTGGCGCGACCACGAGAATGCCAAACGAAATTCCGCCAGCTATCCGGTCCCGCTGTTTGGAGGTTTTTTTCCGCGAGCT
TGAAAAGGACGAGCTGAAAACGGTTGCCAAAACAGCAGCGGATAAAATTGAAAAAAATATCTCCGAAGAAGGACTGGATC
TTTTGACCAGCTACACGCGGAACGGGCGGGAAGCCGTCAATATGATTCAAATCGCAGCGGGGATGGCTGTAACGGAGAAT
CGAAAGGACATTACGATTGAGGATATTGAATGGGTCATTCACTCCAGTCAGCTTACTCCAAAGCACGAGCAAAAAATCGG
TGTTGAACCACAGGTTGGCATTGTAAATGGGCTTGCAGTCTATGGGCCTAACAGCGGATCGCTTCTGGAAATCGAAGTAA
GTGTAACCGCGGCTCAAGATAAAGGATCAATTAACATCACAGGAATAGCTGAAGAAGAAAGCATCGGCAGCCAATCAAAA
TCGATCAGGCGAAAAAGCATGGCGAAGGGTTCGGTTGAAAATGTATTAACCGTGCTCCGGACAATGGGAATGAAGCCTTC
TGATTATGATATTCACATCAATTTCCCAGGAGGGATTCCGATTGATGGGCCGTCAGCAGGCATTGCGATGGCGGCGGGGA
TTTTCTCGGCGATACACAAAATTCCTATTGACAATACCGTTGCGATGACGGGCGAAATCAGCCTGAACGGTCTTGTGAAA
CCCATCGGAGGCGTCATTCCGAAAATAAAAGCGGCCAAGCAGTCCGGTGCGAAAAAGGTGATTATCCCATATGAAAATCA
GCAGGCCATCTTAAAGCAGATTGACGGGATCGAGATTATTGCAGTGAAGACGTTCCAAGAAGTGCTTGATGAAATTTTGG
TGAATCCGCCAACGGAACAAAAACCGTTTCATATCGAAATCAATAAAGAATCCGTTTAA

Upstream 100 bases:

>100_bases
CAACTCTTCCGGAAAAACGTTTATTCCCGTCTTCTATGGGAGATACTAGCAATCAGAGGAATGAATTGGATACATAAATT
GTTTTTCAGGAGGGACCACC

Downstream 100 bases:

>100_bases
ACCCTATTTTGATAATAGGGTTTTTTTCATGAAGGACATCTTTTCCTTTTTCATATCAGAAAGAAAGGGTATACTACGAG
GAGACTGTTTTATAGAAAAT

Product: LonB ATP-dependent protease

Products: NA

Alternate protein names: ATP-dependent protease La 2

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MSWTGIALFIQLFFGIIIGLYFWNLLKNQRTQKVTIDKESKKEMEQLRKMRAISLSEPLSEKVRPKSFKDIVGQEDGIKA
LKAALCGPNPQHVIVYGPPGVGKTAAARLVLEEAKKHKQSPFKEQAVFVELDATTARFDERGIADPLIGSVHDPIYQGAG
AMGQAGIPQPKQGAVTHAHGGVLFIDEIGELHPIQMNKMLKVLEDRKVFLDSAYYSEENTQIPNHIHDIFQNGLPADFRL
IGATTRMPNEIPPAIRSRCLEVFFRELEKDELKTVAKTAADKIEKNISEEGLDLLTSYTRNGREAVNMIQIAAGMAVTEN
RKDITIEDIEWVIHSSQLTPKHEQKIGVEPQVGIVNGLAVYGPNSGSLLEIEVSVTAAQDKGSINITGIAEEESIGSQSK
SIRRKSMAKGSVENVLTVLRTMGMKPSDYDIHINFPGGIPIDGPSAGIAMAAGIFSAIHKIPIDNTVAMTGEISLNGLVK
PIGGVIPKIKAAKQSGAKKVIIPYENQQAILKQIDGIEIIAVKTFQEVLDEILVNPPTEQKPFHIEINKESV

Sequences:

>Translated_552_residues
MSWTGIALFIQLFFGIIIGLYFWNLLKNQRTQKVTIDKESKKEMEQLRKMRAISLSEPLSEKVRPKSFKDIVGQEDGIKA
LKAALCGPNPQHVIVYGPPGVGKTAAARLVLEEAKKHKQSPFKEQAVFVELDATTARFDERGIADPLIGSVHDPIYQGAG
AMGQAGIPQPKQGAVTHAHGGVLFIDEIGELHPIQMNKMLKVLEDRKVFLDSAYYSEENTQIPNHIHDIFQNGLPADFRL
IGATTRMPNEIPPAIRSRCLEVFFRELEKDELKTVAKTAADKIEKNISEEGLDLLTSYTRNGREAVNMIQIAAGMAVTEN
RKDITIEDIEWVIHSSQLTPKHEQKIGVEPQVGIVNGLAVYGPNSGSLLEIEVSVTAAQDKGSINITGIAEEESIGSQSK
SIRRKSMAKGSVENVLTVLRTMGMKPSDYDIHINFPGGIPIDGPSAGIAMAAGIFSAIHKIPIDNTVAMTGEISLNGLVK
PIGGVIPKIKAAKQSGAKKVIIPYENQQAILKQIDGIEIIAVKTFQEVLDEILVNPPTEQKPFHIEINKESV
>Mature_551_residues
SWTGIALFIQLFFGIIIGLYFWNLLKNQRTQKVTIDKESKKEMEQLRKMRAISLSEPLSEKVRPKSFKDIVGQEDGIKAL
KAALCGPNPQHVIVYGPPGVGKTAAARLVLEEAKKHKQSPFKEQAVFVELDATTARFDERGIADPLIGSVHDPIYQGAGA
MGQAGIPQPKQGAVTHAHGGVLFIDEIGELHPIQMNKMLKVLEDRKVFLDSAYYSEENTQIPNHIHDIFQNGLPADFRLI
GATTRMPNEIPPAIRSRCLEVFFRELEKDELKTVAKTAADKIEKNISEEGLDLLTSYTRNGREAVNMIQIAAGMAVTENR
KDITIEDIEWVIHSSQLTPKHEQKIGVEPQVGIVNGLAVYGPNSGSLLEIEVSVTAAQDKGSINITGIAEEESIGSQSKS
IRRKSMAKGSVENVLTVLRTMGMKPSDYDIHINFPGGIPIDGPSAGIAMAAGIFSAIHKIPIDNTVAMTGEISLNGLVKP
IGGVIPKIKAAKQSGAKKVIIPYENQQAILKQIDGIEIIAVKTFQEVLDEILVNPPTEQKPFHIEINKESV

Specific function: ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced

COG id: COG1067

COG function: function code O; Predicted ATP-dependent protease

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S16 family

Homologues:

Organism=Homo sapiens, GI21396489, Length=479, Percent_Identity=25.678496868476, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI31377667, Length=507, Percent_Identity=24.8520710059172, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1786643, Length=485, Percent_Identity=27.4226804123711, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17505831, Length=474, Percent_Identity=27.0042194092827, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556486, Length=493, Percent_Identity=22.920892494929, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319449, Length=115, Percent_Identity=39.1304347826087, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221513036, Length=501, Percent_Identity=25.9481037924152, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24666867, Length=501, Percent_Identity=25.9481037924152, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): LON2_BACSU (P42425)

Other databases:

- EMBL:   U18229
- EMBL:   Z75208
- EMBL:   AL009126
- EMBL:   X76424
- PIR:   G69652
- RefSeq:   NP_390699.1
- ProteinModelPortal:   P42425
- SMR:   P42425
- EnsemblBacteria:   EBBACT00000002052
- GeneID:   937484
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28210
- NMPDR:   fig|224308.1.peg.2824
- GenoList:   BSU28210
- GeneTree:   EBGT00050000000357
- HOGENOM:   HBG399084
- OMA:   QMNKLLK
- ProtClustDB:   CLSK873492
- BioCyc:   BSUB:BSU28210-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008269
- InterPro:   IPR008268
- InterPro:   IPR001984
- InterPro:   IPR020568
- InterPro:   IPR014251
- PRINTS:   PR00830
- SMART:   SM00382
- TIGRFAMs:   TIGR02902

Pfam domain/function: PF00004 AAA; PF05362 Lon_C; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =3.4.21.53

Molecular weight: Translated: 60430; Mature: 60298

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS01046 LON_SER

Important sites: ACT_SITE 445-445 ACT_SITE 488-488

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWTGIALFIQLFFGIIIGLYFWNLLKNQRTQKVTIDKESKKEMEQLRKMRAISLSEPLS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCHH
EKVRPKSFKDIVGQEDGIKALKAALCGPNPQHVIVYGPPGVGKTAAARLVLEEAKKHKQS
HHCCCCHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
PFKEQAVFVELDATTARFDERGIADPLIGSVHDPIYQGAGAMGQAGIPQPKQGAVTHAHG
CCCCCEEEEEECCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCC
GVLFIDEIGELHPIQMNKMLKVLEDRKVFLDSAYYSEENTQIPNHIHDIFQNGLPADFRL
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEE
IGATTRMPNEIPPAIRSRCLEVFFRELEKDELKTVAKTAADKIEKNISEEGLDLLTSYTR
EECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NGREAVNMIQIAAGMAVTENRKDITIEDIEWVIHSSQLTPKHEQKIGVEPQVGIVNGLAV
CCHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCEEECCEEE
YGPNSGSLLEIEVSVTAAQDKGSINITGIAEEESIGSQSKSIRRKSMAKGSVENVLTVLR
EECCCCCEEEEEEEEEEECCCCCEEEEEECCHHHCCHHHHHHHHHHHHCCCHHHHHHHHH
TMGMKPSDYDIHINFPGGIPIDGPSAGIAMAAGIFSAIHKIPIDNTVAMTGEISLNGLVK
HHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHH
PIGGVIPKIKAAKQSGAKKVIIPYENQQAILKQIDGIEIIAVKTFQEVLDEILVNPPTEQ
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCC
KPFHIEINKESV
CCEEEEECCCCC
>Mature Secondary Structure 
SWTGIALFIQLFFGIIIGLYFWNLLKNQRTQKVTIDKESKKEMEQLRKMRAISLSEPLS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCHH
EKVRPKSFKDIVGQEDGIKALKAALCGPNPQHVIVYGPPGVGKTAAARLVLEEAKKHKQS
HHCCCCHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
PFKEQAVFVELDATTARFDERGIADPLIGSVHDPIYQGAGAMGQAGIPQPKQGAVTHAHG
CCCCCEEEEEECCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCC
GVLFIDEIGELHPIQMNKMLKVLEDRKVFLDSAYYSEENTQIPNHIHDIFQNGLPADFRL
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEE
IGATTRMPNEIPPAIRSRCLEVFFRELEKDELKTVAKTAADKIEKNISEEGLDLLTSYTR
EECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NGREAVNMIQIAAGMAVTENRKDITIEDIEWVIHSSQLTPKHEQKIGVEPQVGIVNGLAV
CCHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCEEECCEEE
YGPNSGSLLEIEVSVTAAQDKGSINITGIAEEESIGSQSKSIRRKSMAKGSVENVLTVLR
EECCCCCEEEEEEEEEEECCCCCEEEEEECCHHHCCHHHHHHHHHHHHCCCHHHHHHHHH
TMGMKPSDYDIHINFPGGIPIDGPSAGIAMAAGIFSAIHKIPIDNTVAMTGEISLNGLVK
HHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHH
PIGGVIPKIKAAKQSGAKKVIIPYENQQAILKQIDGIEIIAVKTFQEVLDEILVNPPTEQ
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCC
KPFHIEINKESV
CCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 7961402; 11325926