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The map label for this gene is lonA

Identifier: 16079872

GI number: 16079872

Start: 2880466

End: 2882790

Strand: Reverse

Name: lonA

Synonym: BSU28200

Alternate gene names: 16079872

Gene position: 2882790-2880466 (Counterclockwise)

Preceding gene: 16079873

Following gene: 16079871

Centisome position: 68.38

GC content: 44.73

Gene sequence:

>2325_bases
ATGGCAGAAGAATTAAAACGCAGCATCCCGCTCCTCCCTTTAAGAGGTTTATTAGTGTACCCGACGATGGTTTTGCATTT
AGATGTAGGACGTGACAAGTCGGTTCAGGCTCTTGAACAGGCAATGATGCATGATCATATGATATTTTTAGCCACTCAGC
AGGATATTTCGATAGACGAACCTGGTGAAGACGAGATTTTCACTGTCGGCACCTATACAAAAATTAAGCAAATGCTGAAG
CTTCCGAACGGTACGATTCGTGTGTTGGTGGAGGGCCTCAAGCGCGCCCATATCGTCAAATACAATGAACACGAGGATTA
CACGTCGGTTGACATTCAACTTATTCATGAAGATGATTCAAAAGATACAGAAGACGAAGCATTGATGCGGACTTTGCTAG
ACCACTTTGATCAGTACATAAAAATTTCTAAAAAAATCTCGGCCGAAACGTACGCTGCGGTTACTGATATCGAAGAGCCG
GGGAGAATGGCAGACATCGTTGCTTCTCATCTGCCCCTTAAACTAAAGGATAAGCAGGATATTTTAGAAACGGCTGATGT
CAAAGACAGGCTGAACAAGGTCATTGATTTTATTAATAATGAAAAAGAAGTGCTGGAGATTGAGAAAAAAATCGGCCAGC
GCGTCAAACGTTCGATGGAGCGTACGCAGAAGGAATACTACTTGCGTGAACAAATGAAGGCGATCCAAAAAGAGCTTGGC
GATAAAGAAGGCAAAACTGGCGAAGTTCAGACGCTGACGGAAAAAATCGAAGAAGCAGGCATGCCTGATCATGTGAAAGA
AACAGCGCTTAAAGAATTGAACAGATATGAAAAAATTCCGTCAAGTTCTGCGGAAAGCTCTGTGATCCGCAACTATATCG
ACTGGCTGGTTGCTCTTCCGTGGACGGATGAAACAGATGATAAGCTTGATTTGAAAGAAGCAGGACGTCTCTTGGACGAA
GAGCACCACGGGCTTGAAAAAGTAAAAGAACGTATTTTAGAATATTTGGCTGTCCAGAAGCTGACAAAATCCCTGAAAGG
CCCGATTCTCTGTTTGGCAGGACCTCCAGGTGTCGGAAAAACGTCTTTAGCCAAATCAATTGCAAAAAGCTTGGGACGCA
AATTCGTCAGAATCTCACTCGGAGGAGTTCGGGATGAATCAGAGATACGCGGACACAGACGGACCTATGTCGGAGCAATG
CCTGGACGTATTATTCAAGGGATGAAAAAAGCGGGCAAGCTGAATCCGGTCTTCTTATTAGACGAAATTGACAAAATGTC
ATCTGATTTTAGAGGAGACCCATCATCCGCTATGCTTGAAGTGCTTGATCCAGAGCAAAACAGCAGCTTCAGTGATCACT
ATATTGAAGAAACCTTTGATTTATCCAAAGTGCTGTTTATCGCAACGGCGAACAATTTGGCCACAATCCCGGGCCCGCTC
AGAGACAGAATGGAAATTATTAATATCGCAGGCTACACAGAAATAGAAAAACTTGAAATTGTAAAGGATCACTTGCTTCC
AAAGCAAATCAAAGAACACGGGCTGAAGAAAAGCAATCTTCAGCTGCGTGATCAGGCGATTCTTGATATTATTCGTTACT
ATACGAGAGAAGCGGGTGTAAGAAGCCTTGAACGCCAGCTTGCCGCAATTTGCCGTAAAGCTGCAAAAGCCATTGTTGCT
GAAGAGCGAAAACGAATTACGGTTACTGAGAAGAACCTTCAAGATTTTATCGGAAAACGCATTTTCAGATATGGACAAGC
TGAAACAGAGGATCAAGTTGGTGTAGTGACAGGGCTTGCGTATACAACCGTTGGCGGAGATACGCTTTCGATTGAAGTAT
CGCTTTCACCGGGAAAAGGGAAATTAATCCTGACAGGAAAGCTCGGAGATGTTATGAGAGAGTCTGCTCAGGCTGCATTC
AGCTATGTGCGATCCAAAACAGAAGAACTTGGCATTGAACCTGACTTTCATGAGAAGTATGATATTCATATACATGTACC
AGAGGGTGCGGTGCCAAAAGATGGTCCGTCAGCCGGTATTACGATGGCAACAGCGCTTGTTTCTGCTTTAACGGGACGGG
CGGTTTCGCGTGAAGTCGGCATGACTGGGGAAATCACGCTTCGCGGGCGCGTGCTGCCGATCGGCGGCTTAAAGGAAAAA
GCGCTTGGCGCACATAGAGCGGGATTAACGACCATTATTGCGCCTAAAGATAATGAAAAAGATATAGAGGATATTCCGGA
AAGCGTCAGGGAGGGGCTGACATTTATATTGGCGTCCCACTTAGACGAGGTTTTGGAACATGCCTTAGTAGGAGAGAAGA
AATGA

Upstream 100 bases:

>100_bases
GAGACTGTTTTATAGAAAATGGAACCATGCCGTTTATTGTACAAGGATGAAAAAGTTGTATTATAATGGTTCATACTAAA
GTCACGGAGGTGTCAGTCAC

Downstream 100 bases:

>100_bases
AAGTCACAAAGTCAGAAATCGTGATCAGTGCAGTAAAACCGGAACAGTACCCTGAAGGGGGGCTTCCGGAAATCGCATTG
GCCGGAAGATCGAACGTAGG

Product: class III heat-shock ATP-dependent LonA protease

Products: NA

Alternate protein names: ATP-dependent protease La 1

Number of amino acids: Translated: 774; Mature: 773

Protein sequence:

>774_residues
MAEELKRSIPLLPLRGLLVYPTMVLHLDVGRDKSVQALEQAMMHDHMIFLATQQDISIDEPGEDEIFTVGTYTKIKQMLK
LPNGTIRVLVEGLKRAHIVKYNEHEDYTSVDIQLIHEDDSKDTEDEALMRTLLDHFDQYIKISKKISAETYAAVTDIEEP
GRMADIVASHLPLKLKDKQDILETADVKDRLNKVIDFINNEKEVLEIEKKIGQRVKRSMERTQKEYYLREQMKAIQKELG
DKEGKTGEVQTLTEKIEEAGMPDHVKETALKELNRYEKIPSSSAESSVIRNYIDWLVALPWTDETDDKLDLKEAGRLLDE
EHHGLEKVKERILEYLAVQKLTKSLKGPILCLAGPPGVGKTSLAKSIAKSLGRKFVRISLGGVRDESEIRGHRRTYVGAM
PGRIIQGMKKAGKLNPVFLLDEIDKMSSDFRGDPSSAMLEVLDPEQNSSFSDHYIEETFDLSKVLFIATANNLATIPGPL
RDRMEIINIAGYTEIEKLEIVKDHLLPKQIKEHGLKKSNLQLRDQAILDIIRYYTREAGVRSLERQLAAICRKAAKAIVA
EERKRITVTEKNLQDFIGKRIFRYGQAETEDQVGVVTGLAYTTVGGDTLSIEVSLSPGKGKLILTGKLGDVMRESAQAAF
SYVRSKTEELGIEPDFHEKYDIHIHVPEGAVPKDGPSAGITMATALVSALTGRAVSREVGMTGEITLRGRVLPIGGLKEK
ALGAHRAGLTTIIAPKDNEKDIEDIPESVREGLTFILASHLDEVLEHALVGEKK

Sequences:

>Translated_774_residues
MAEELKRSIPLLPLRGLLVYPTMVLHLDVGRDKSVQALEQAMMHDHMIFLATQQDISIDEPGEDEIFTVGTYTKIKQMLK
LPNGTIRVLVEGLKRAHIVKYNEHEDYTSVDIQLIHEDDSKDTEDEALMRTLLDHFDQYIKISKKISAETYAAVTDIEEP
GRMADIVASHLPLKLKDKQDILETADVKDRLNKVIDFINNEKEVLEIEKKIGQRVKRSMERTQKEYYLREQMKAIQKELG
DKEGKTGEVQTLTEKIEEAGMPDHVKETALKELNRYEKIPSSSAESSVIRNYIDWLVALPWTDETDDKLDLKEAGRLLDE
EHHGLEKVKERILEYLAVQKLTKSLKGPILCLAGPPGVGKTSLAKSIAKSLGRKFVRISLGGVRDESEIRGHRRTYVGAM
PGRIIQGMKKAGKLNPVFLLDEIDKMSSDFRGDPSSAMLEVLDPEQNSSFSDHYIEETFDLSKVLFIATANNLATIPGPL
RDRMEIINIAGYTEIEKLEIVKDHLLPKQIKEHGLKKSNLQLRDQAILDIIRYYTREAGVRSLERQLAAICRKAAKAIVA
EERKRITVTEKNLQDFIGKRIFRYGQAETEDQVGVVTGLAYTTVGGDTLSIEVSLSPGKGKLILTGKLGDVMRESAQAAF
SYVRSKTEELGIEPDFHEKYDIHIHVPEGAVPKDGPSAGITMATALVSALTGRAVSREVGMTGEITLRGRVLPIGGLKEK
ALGAHRAGLTTIIAPKDNEKDIEDIPESVREGLTFILASHLDEVLEHALVGEKK
>Mature_773_residues
AEELKRSIPLLPLRGLLVYPTMVLHLDVGRDKSVQALEQAMMHDHMIFLATQQDISIDEPGEDEIFTVGTYTKIKQMLKL
PNGTIRVLVEGLKRAHIVKYNEHEDYTSVDIQLIHEDDSKDTEDEALMRTLLDHFDQYIKISKKISAETYAAVTDIEEPG
RMADIVASHLPLKLKDKQDILETADVKDRLNKVIDFINNEKEVLEIEKKIGQRVKRSMERTQKEYYLREQMKAIQKELGD
KEGKTGEVQTLTEKIEEAGMPDHVKETALKELNRYEKIPSSSAESSVIRNYIDWLVALPWTDETDDKLDLKEAGRLLDEE
HHGLEKVKERILEYLAVQKLTKSLKGPILCLAGPPGVGKTSLAKSIAKSLGRKFVRISLGGVRDESEIRGHRRTYVGAMP
GRIIQGMKKAGKLNPVFLLDEIDKMSSDFRGDPSSAMLEVLDPEQNSSFSDHYIEETFDLSKVLFIATANNLATIPGPLR
DRMEIINIAGYTEIEKLEIVKDHLLPKQIKEHGLKKSNLQLRDQAILDIIRYYTREAGVRSLERQLAAICRKAAKAIVAE
ERKRITVTEKNLQDFIGKRIFRYGQAETEDQVGVVTGLAYTTVGGDTLSIEVSLSPGKGKLILTGKLGDVMRESAQAAFS
YVRSKTEELGIEPDFHEKYDIHIHVPEGAVPKDGPSAGITMATALVSALTGRAVSREVGMTGEITLRGRVLPIGGLKEKA
LGAHRAGLTTIIAPKDNEKDIEDIPESVREGLTFILASHLDEVLEHALVGEKK

Specific function: ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced

COG id: COG0466

COG function: function code O; ATP-dependent Lon protease, bacterial type

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Lon domain

Homologues:

Organism=Homo sapiens, GI31377667, Length=750, Percent_Identity=41.8666666666667, Blast_Score=569, Evalue=1e-162,
Organism=Homo sapiens, GI21396489, Length=829, Percent_Identity=37.7563329312425, Blast_Score=555, Evalue=1e-158,
Organism=Escherichia coli, GI1786643, Length=765, Percent_Identity=55.8169934640523, Blast_Score=873, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17505831, Length=688, Percent_Identity=39.8255813953488, Blast_Score=505, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17556486, Length=780, Percent_Identity=36.1538461538462, Blast_Score=468, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6319449, Length=682, Percent_Identity=40.6158357771261, Blast_Score=525, Evalue=1e-150,
Organism=Drosophila melanogaster, GI221513036, Length=625, Percent_Identity=43.84, Blast_Score=533, Evalue=1e-151,
Organism=Drosophila melanogaster, GI24666867, Length=625, Percent_Identity=43.84, Blast_Score=533, Evalue=1e-151,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): LON1_BACSU (P37945)

Other databases:

- EMBL:   X76424
- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   I40421
- RefSeq:   NP_390698.1
- PDB:   1X37
- PDB:   3M65
- PDB:   3M6A
- PDBsum:   1X37
- PDBsum:   3M65
- PDBsum:   3M6A
- ProteinModelPortal:   P37945
- MEROPS:   S16.001
- EnsemblBacteria:   EBBACT00000002229
- GeneID:   937486
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28200
- NMPDR:   fig|224308.1.peg.2823
- GenoList:   BSU28200
- GeneTree:   EBGT00050000000357
- HOGENOM:   HBG566281
- OMA:   IDPDFHE
- PhylomeDB:   P37945
- ProtClustDB:   CLSK2518255
- BioCyc:   BSUB:BSU28200-MONOMER
- BRENDA:   3.4.21.53
- GO:   GO:0005737
- GO:   GO:0006508
- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008269
- InterPro:   IPR004815
- InterPro:   IPR003111
- InterPro:   IPR008268
- InterPro:   IPR001984
- InterPro:   IPR015947
- InterPro:   IPR020568
- PRINTS:   PR00830
- SMART:   SM00382
- SMART:   SM00464
- TIGRFAMs:   TIGR00763

Pfam domain/function: PF00004 AAA; PF02190 LON; PF05362 Lon_C; SSF88697 PUA-like; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =3.4.21.53

Molecular weight: Translated: 86608; Mature: 86477

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS01046 LON_SER

Important sites: ACT_SITE 677-677 ACT_SITE 720-720

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEELKRSIPLLPLRGLLVYPTMVLHLDVGRDKSVQALEQAMMHDHMIFLATQQDISIDE
CCHHHHHCCCCCCCCHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCC
PGEDEIFTVGTYTKIKQMLKLPNGTIRVLVEGLKRAHIVKYNEHEDYTSVDIQLIHEDDS
CCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEECCCC
KDTEDEALMRTLLDHFDQYIKISKKISAETYAAVTDIEEPGRMADIVASHLPLKLKDKQD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEECCHHH
ILETADVKDRLNKVIDFINNEKEVLEIEKKIGQRVKRSMERTQKEYYLREQMKAIQKELG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKEGKTGEVQTLTEKIEEAGMPDHVKETALKELNRYEKIPSSSAESSVIRNYIDWLVALP
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECC
WTDETDDKLDLKEAGRLLDEEHHGLEKVKERILEYLAVQKLTKSLKGPILCLAGPPGVGK
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
TSLAKSIAKSLGRKFVRISLGGVRDESEIRGHRRTYVGAMPGRIIQGMKKAGKLNPVFLL
HHHHHHHHHHHHHHEEEEEECCCCCHHHHCCCHHHEECCCCHHHHHHHHHHCCCCCEEEH
DEIDKMSSDFRGDPSSAMLEVLDPEQNSSFSDHYIEETFDLSKVLFIATANNLATIPGPL
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCH
RDRMEIINIAGYTEIEKLEIVKDHLLPKQIKEHGLKKSNLQLRDQAILDIIRYYTREAGV
HHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RSLERQLAAICRKAAKAIVAEERKRITVTEKNLQDFIGKRIFRYGQAETEDQVGVVTGLA
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHE
YTTVGGDTLSIEVSLSPGKGKLILTGKLGDVMRESAQAAFSYVRSKTEELGIEPDFHEKY
EEEECCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
DIHIHVPEGAVPKDGPSAGITMATALVSALTGRAVSREVGMTGEITLRGRVLPIGGLKEK
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHH
ALGAHRAGLTTIIAPKDNEKDIEDIPESVREGLTFILASHLDEVLEHALVGEKK
HHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AEELKRSIPLLPLRGLLVYPTMVLHLDVGRDKSVQALEQAMMHDHMIFLATQQDISIDE
CHHHHHCCCCCCCCHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCC
PGEDEIFTVGTYTKIKQMLKLPNGTIRVLVEGLKRAHIVKYNEHEDYTSVDIQLIHEDDS
CCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEECCCC
KDTEDEALMRTLLDHFDQYIKISKKISAETYAAVTDIEEPGRMADIVASHLPLKLKDKQD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEECCHHH
ILETADVKDRLNKVIDFINNEKEVLEIEKKIGQRVKRSMERTQKEYYLREQMKAIQKELG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DKEGKTGEVQTLTEKIEEAGMPDHVKETALKELNRYEKIPSSSAESSVIRNYIDWLVALP
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECC
WTDETDDKLDLKEAGRLLDEEHHGLEKVKERILEYLAVQKLTKSLKGPILCLAGPPGVGK
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
TSLAKSIAKSLGRKFVRISLGGVRDESEIRGHRRTYVGAMPGRIIQGMKKAGKLNPVFLL
HHHHHHHHHHHHHHEEEEEECCCCCHHHHCCCHHHEECCCCHHHHHHHHHHCCCCCEEEH
DEIDKMSSDFRGDPSSAMLEVLDPEQNSSFSDHYIEETFDLSKVLFIATANNLATIPGPL
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCH
RDRMEIINIAGYTEIEKLEIVKDHLLPKQIKEHGLKKSNLQLRDQAILDIIRYYTREAGV
HHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RSLERQLAAICRKAAKAIVAEERKRITVTEKNLQDFIGKRIFRYGQAETEDQVGVVTGLA
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHE
YTTVGGDTLSIEVSLSPGKGKLILTGKLGDVMRESAQAAFSYVRSKTEELGIEPDFHEKY
EEEECCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
DIHIHVPEGAVPKDGPSAGITMATALVSALTGRAVSREVGMTGEITLRGRVLPIGGLKEK
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHH
ALGAHRAGLTTIIAPKDNEKDIEDIPESVREGLTFILASHLDEVLEHALVGEKK
HHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961402; 8969504; 9384377