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The map label for this gene is hemA

Identifier: 16079869

GI number: 16079869

Start: 2877766

End: 2879133

Strand: Reverse

Name: hemA

Synonym: BSU28170

Alternate gene names: 16079869

Gene position: 2879133-2877766 (Counterclockwise)

Preceding gene: 16079871

Following gene: 255767646

Centisome position: 68.3

GC content: 43.93

Gene sequence:

>1368_bases
ATGCATATACTTGTTGTGGGAGTAGATTATAAATCCGCCCCTATTGAGATACGTGAAAAAGTAAGTTTTCAGCCGAATGA
GCTGGCAGAAGCAATGGTGCAGCTGAAAGAAGAGAAAAGCATTCTTGAAAACATCATTGTCTCAACCTGCAACCGCACTG
AAATTTATGCGGTAGTCGACCAGCTTCATACCGGCCGTTATTATATAAAAAAGTTTTTAGCTGATTGGTTTCAATTAAGC
AAAGAAGAGCTGTCACCGTTCTTAACGTTTTATGAGAGCGATGCCGCTGTTGAGCATTTATTCCGTGTAGCCTGCGGACT
TGATTCTATGGTGATTGGCGAAACGCAGATTCTCGGACAGGTACGCGACAGCTTTAAAACAGCTCAGCAAGAAAAAACGA
TCGGGACTATTTTTAATGAGCTGTTTAAGCAGGCAGTTACAGTGGGCAAACGGACTCACGCCGAAACAGACATTGGCTCA
AATGCGGTGTCAGTAAGCTATGCTGCAGTTGAACTTGCCAAAAAAATCTTCGGAAATCTTTCAAGCAAGCACATATTGAT
TCTCGGTGCGGGAAAAATGGGCGAGCTTGCTGCGGAAAACCTGCACGGACAGGGAATCGGCAAGGTCACTGTCATTAACC
GAACATACTTGAAAGCGAAGGAGCTTGCAGACCGTTTTTCAGGTGAAGCGAGAAGCTTGAATCAGCTTGAAAGCGCGCTT
GCGGAGGCTGATATTTTAATCAGTTCAACCGGTGCAAGTGAATTTGTCGTGTCCAAAGAGATGATGGAAAACGCGAATAA
GCTTCGCAAGGGACGTCCGCTGTTTATGGTCGACATTGCCGTGCCTAGAGATCTTGATCCGGCGCTGAATGATCTTGAAG
GTGTTTTTCTTTATGATATCGACGATCTGGAAGGCATTGTAGAAGCGAACATGAAAGAGCGGAGAGAAACAGCTGAAAAA
GTTGAACTGTTAATTGAAGAAACCATTGTGGAATTTAAACAATGGATGAATACACTTGGTGTTGTGCCTGTTATTTCTGC
ATTGCGCGAAAAGGCGCTTGCCATCCAGTCAGAAACGATGGACAGCATTGAGCGTAAGCTGCCTCACTTAAGCACAAGAG
AGAAAAAACTGTTGAACAAACACACCAAAAGTATTATTAACCAAATGCTTCGTGATCCGATTTTAAAGGTGAAAGAGCTT
GCGGCAGATGCTGATTCTGAAGAAAAGCTCGCGTTGTTTATGCAGATTTTTGATATTGAAGAAGCTGCGGGCCGTCAAAT
GATGAAAACCGTTGAAAGCAGCCAGAAGGTCCACTCTTTTAAGAAGGCTGAATCAAAAGCGGGCTTTAGCCCACTTGTAA
GTGAGTGA

Upstream 100 bases:

>100_bases
CATTTTGTGAAAGAAACTATGTTATAATTATTATAAATAATGAGTTCTATGTTAGAATGATTATAAATTAAGATTGGGTG
TTGGGGGTGTAATTAGAGCG

Downstream 100 bases:

>100_bases
AAGCTGAATGATTGATACTGCAATGGCAAGACTTAATGAGGGGACAATCGTCATTTACGCGTTAAGTGTACTCTTTTATT
TTATAGATTTTCTTCAACAC

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MHILVVGVDYKSAPIEIREKVSFQPNELAEAMVQLKEEKSILENIIVSTCNRTEIYAVVDQLHTGRYYIKKFLADWFQLS
KEELSPFLTFYESDAAVEHLFRVACGLDSMVIGETQILGQVRDSFKTAQQEKTIGTIFNELFKQAVTVGKRTHAETDIGS
NAVSVSYAAVELAKKIFGNLSSKHILILGAGKMGELAAENLHGQGIGKVTVINRTYLKAKELADRFSGEARSLNQLESAL
AEADILISSTGASEFVVSKEMMENANKLRKGRPLFMVDIAVPRDLDPALNDLEGVFLYDIDDLEGIVEANMKERRETAEK
VELLIEETIVEFKQWMNTLGVVPVISALREKALAIQSETMDSIERKLPHLSTREKKLLNKHTKSIINQMLRDPILKVKEL
AADADSEEKLALFMQIFDIEEAAGRQMMKTVESSQKVHSFKKAESKAGFSPLVSE

Sequences:

>Translated_455_residues
MHILVVGVDYKSAPIEIREKVSFQPNELAEAMVQLKEEKSILENIIVSTCNRTEIYAVVDQLHTGRYYIKKFLADWFQLS
KEELSPFLTFYESDAAVEHLFRVACGLDSMVIGETQILGQVRDSFKTAQQEKTIGTIFNELFKQAVTVGKRTHAETDIGS
NAVSVSYAAVELAKKIFGNLSSKHILILGAGKMGELAAENLHGQGIGKVTVINRTYLKAKELADRFSGEARSLNQLESAL
AEADILISSTGASEFVVSKEMMENANKLRKGRPLFMVDIAVPRDLDPALNDLEGVFLYDIDDLEGIVEANMKERRETAEK
VELLIEETIVEFKQWMNTLGVVPVISALREKALAIQSETMDSIERKLPHLSTREKKLLNKHTKSIINQMLRDPILKVKEL
AADADSEEKLALFMQIFDIEEAAGRQMMKTVESSQKVHSFKKAESKAGFSPLVSE
>Mature_455_residues
MHILVVGVDYKSAPIEIREKVSFQPNELAEAMVQLKEEKSILENIIVSTCNRTEIYAVVDQLHTGRYYIKKFLADWFQLS
KEELSPFLTFYESDAAVEHLFRVACGLDSMVIGETQILGQVRDSFKTAQQEKTIGTIFNELFKQAVTVGKRTHAETDIGS
NAVSVSYAAVELAKKIFGNLSSKHILILGAGKMGELAAENLHGQGIGKVTVINRTYLKAKELADRFSGEARSLNQLESAL
AEADILISSTGASEFVVSKEMMENANKLRKGRPLFMVDIAVPRDLDPALNDLEGVFLYDIDDLEGIVEANMKERRETAEK
VELLIEETIVEFKQWMNTLGVVPVISALREKALAIQSETMDSIERKLPHLSTREKKLLNKHTKSIINQMLRDPILKVKEL
AADADSEEKLALFMQIFDIEEAAGRQMMKTVESSQKVHSFKKAESKAGFSPLVSE

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=407, Percent_Identity=34.6437346437346, Blast_Score=260, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_BACSU (P16618)

Other databases:

- EMBL:   M57676
- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   A35252
- RefSeq:   NP_390695.1
- ProteinModelPortal:   P16618
- SMR:   P16618
- EnsemblBacteria:   EBBACT00000003499
- GeneID:   937443
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28170
- NMPDR:   fig|224308.1.peg.2820
- GenoList:   BSU28170
- GeneTree:   EBGT00050000001402
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- PhylomeDB:   P16618
- ProtClustDB:   PRK00045
- BioCyc:   BSUB:BSU28170-MONOMER
- BRENDA:   1.2.1.70
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR003462
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR13812
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 50844; Mature: 50844

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILVVGVDYKSAPIEIREKVSFQPNELAEAMVQLKEEKSILENIIVSTCNRTEIYAVVD
CEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QLHTGRYYIKKFLADWFQLSKEELSPFLTFYESDAAVEHLFRVACGLDSMVIGETQILGQ
HHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VRDSFKTAQQEKTIGTIFNELFKQAVTVGKRTHAETDIGSNAVSVSYAAVELAKKIFGNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
SSKHILILGAGKMGELAAENLHGQGIGKVTVINRTYLKAKELADRFSGEARSLNQLESAL
CCCEEEEEECCCHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHH
AEADILISSTGASEFVVSKEMMENANKLRKGRPLFMVDIAVPRDLDPALNDLEGVFLYDI
HHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHCCCCEEEEEC
DDLEGIVEANMKERRETAEKVELLIEETIVEFKQWMNTLGVVPVISALREKALAIQSETM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
DSIERKLPHLSTREKKLLNKHTKSIINQMLRDPILKVKELAADADSEEKLALFMQIFDIE
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EAAGRQMMKTVESSQKVHSFKKAESKAGFSPLVSE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHILVVGVDYKSAPIEIREKVSFQPNELAEAMVQLKEEKSILENIIVSTCNRTEIYAVVD
CEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QLHTGRYYIKKFLADWFQLSKEELSPFLTFYESDAAVEHLFRVACGLDSMVIGETQILGQ
HHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VRDSFKTAQQEKTIGTIFNELFKQAVTVGKRTHAETDIGSNAVSVSYAAVELAKKIFGNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
SSKHILILGAGKMGELAAENLHGQGIGKVTVINRTYLKAKELADRFSGEARSLNQLESAL
CCCEEEEEECCCHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHH
AEADILISSTGASEFVVSKEMMENANKLRKGRPLFMVDIAVPRDLDPALNDLEGVFLYDI
HHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHCCCCEEEEEC
DDLEGIVEANMKERRETAEKVELLIEETIVEFKQWMNTLGVVPVISALREKALAIQSETM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
DSIERKLPHLSTREKKLLNKHTKSIINQMLRDPILKVKELAADADSEEKLALFMQIFDIE
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EAAGRQMMKTVESSQKVHSFKKAESKAGFSPLVSE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2110138; 8969504; 9384377