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The map label for this gene is spoIVFA

Identifier: 16079850

GI number: 16079850

Start: 2856832

End: 2857626

Strand: Reverse

Name: spoIVFA

Synonym: BSU27980

Alternate gene names: 16079850

Gene position: 2857626-2856832 (Counterclockwise)

Preceding gene: 16079851

Following gene: 16079849

Centisome position: 67.79

GC content: 42.14

Gene sequence:

>795_bases
ATGAGTCACAGAGCAGATGAAATCAGAAAACGATTAGAGAAAAGAAGAAAGCAGCTTTCCGGCTCAAAACGTTTCTCTAC
TCAGACAGTTTCTGAAAAGCAGAAACCCCCGTCCTGGGTGATGGTAACGGATCAGGAAAAGCATGGAACACTTCCGGTCT
ACGAAGATAACATGCCAACATTCAACGGAAAACACCCATTGGTGAAAACAGATTCAATTATCCTGAAATGTCTTCTGTCG
GCCTGCCTTGTTCTCGTTTCAGCTATAGCCTATAAAACAAACATTGGACCCGTCAGTCAGATTAAACCCGCCGTAGCCAA
AACCTTTGAAACTGAATTTCAATTTGCTTCAGCAAGCCATTGGTTCGAAACCAAATTCGGAAATCCGCTTGCTTTCCTGG
CTCCTGAACACAAAAATAAGGAACAGCAGATTGAAGTAGGCAAAGATCTGATCGCGCCTGCATCCGGGAAAGTACAGCAG
GATTTTCAGGACAATGGGGAAGGAATTAAAGTCGAAACAAGCAGTGATAAGATTGATAGCGTAAAAGAAGGCTATGTGGT
TGAAGTCAGCAAAGACAGCCAAACGGGACTGACGGTTAAGGTGCAGCATGCTGACAACACCTATAGTATCTATGGCGAGC
TCAAAGATGTGGATGTTGCTTTATATGATTTTGTGGATAAAGGCAAAAAGCTCGGTTCGATTAAGCTTGATGATCATAAT
AAAGGGGTCTATTATTTTGCCATGAAAGACGGCGATAAATTTATTGATCCGATTCAGGTGATTTCATTTGAATAA

Upstream 100 bases:

>100_bases
TTTTTTTTGTTTTTGCCTGCTTTGTTCTTGACTAAACCGAATATTTGCCATGGACAAGACATATGATGTACAAACCCAAC
GAATGCAAAGGATGATGGCA

Downstream 100 bases:

>100_bases
ATGGCTCGACCTTATCTTAAAGATCCATGTGCATCCTTTTCTTTGGATTATTGCGGCGCTGGGCTTGCTCACAGGCCATA
TGAAAGCATTATTATGTCTG

Product: regulator of SpoIVFB (stage IV sporulation)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSHRADEIRKRLEKRRKQLSGSKRFSTQTVSEKQKPPSWVMVTDQEKHGTLPVYEDNMPTFNGKHPLVKTDSIILKCLLS
ACLVLVSAIAYKTNIGPVSQIKPAVAKTFETEFQFASASHWFETKFGNPLAFLAPEHKNKEQQIEVGKDLIAPASGKVQQ
DFQDNGEGIKVETSSDKIDSVKEGYVVEVSKDSQTGLTVKVQHADNTYSIYGELKDVDVALYDFVDKGKKLGSIKLDDHN
KGVYYFAMKDGDKFIDPIQVISFE

Sequences:

>Translated_264_residues
MSHRADEIRKRLEKRRKQLSGSKRFSTQTVSEKQKPPSWVMVTDQEKHGTLPVYEDNMPTFNGKHPLVKTDSIILKCLLS
ACLVLVSAIAYKTNIGPVSQIKPAVAKTFETEFQFASASHWFETKFGNPLAFLAPEHKNKEQQIEVGKDLIAPASGKVQQ
DFQDNGEGIKVETSSDKIDSVKEGYVVEVSKDSQTGLTVKVQHADNTYSIYGELKDVDVALYDFVDKGKKLGSIKLDDHN
KGVYYFAMKDGDKFIDPIQVISFE
>Mature_263_residues
SHRADEIRKRLEKRRKQLSGSKRFSTQTVSEKQKPPSWVMVTDQEKHGTLPVYEDNMPTFNGKHPLVKTDSIILKCLLSA
CLVLVSAIAYKTNIGPVSQIKPAVAKTFETEFQFASASHWFETKFGNPLAFLAPEHKNKEQQIEVGKDLIAPASGKVQQD
FQDNGEGIKVETSSDKIDSVKEGYVVEVSKDSQTGLTVKVQHADNTYSIYGELKDVDVALYDFVDKGKKLGSIKLDDHNK
GVYYFAMKDGDKFIDPIQVISFE

Specific function: Implicated in the coupling of mother cell to forespore gene expression. Required for spore formation at 37 degrees Celsius, but not at 30 degrees Celsius. SpoIVFA plays a central role in both maintaining the SpoIVFA/BofA/SpoIVFB complex and anchoring it t

COG id: COG0739

COG function: function code M; Membrane proteins related to metalloendopeptidases

Gene ontology:

Cell location: Forespore outer membrane

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SP4FA_BACSU (P26936)

Other databases:

- EMBL:   X59528
- EMBL:   AL009126
- EMBL:   Z15113
- PIR:   S18437
- RefSeq:   NP_390676.1
- ProteinModelPortal:   P26936
- SMR:   P26936
- EnsemblBacteria:   EBBACT00000003728
- GeneID:   937501
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27980
- NMPDR:   fig|224308.1.peg.2801
- GenoList:   BSU27980
- GeneTree:   EBGT00050000002766
- HOGENOM:   HBG314105
- OMA:   EFQFAAV
- ProtClustDB:   CLSK873481
- BioCyc:   BSUB:BSU27980-MONOMER
- InterPro:   IPR011055
- InterPro:   IPR016047

Pfam domain/function: PF01551 Peptidase_M23; SSF51261 Dup_hybrid_motif

EC number: NA

Molecular weight: Translated: 29607; Mature: 29476

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x116ee2c0)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRADEIRKRLEKRRKQLSGSKRFSTQTVSEKQKPPSWVMVTDQEKHGTLPVYEDNMPT
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCEEECCCCC
FNGKHPLVKTDSIILKCLLSACLVLVSAIAYKTNIGPVSQIKPAVAKTFETEFQFASASH
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCC
WFETKFGNPLAFLAPEHKNKEQQIEVGKDLIAPASGKVQQDFQDNGEGIKVETSSDKIDS
EEECCCCCEEEEECCCCCCCHHHHHHCHHHCCCCCCCCHHHHHCCCCEEEEECCCCHHHH
VKEGYVVEVSKDSQTGLTVKVQHADNTYSIYGELKDVDVALYDFVDKGKKLGSIKLDDHN
CCCCEEEEECCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCC
KGVYYFAMKDGDKFIDPIQVISFE
CCEEEEEECCCCCCCCCEEEEECH
>Mature Secondary Structure 
SHRADEIRKRLEKRRKQLSGSKRFSTQTVSEKQKPPSWVMVTDQEKHGTLPVYEDNMPT
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCEEECCCCC
FNGKHPLVKTDSIILKCLLSACLVLVSAIAYKTNIGPVSQIKPAVAKTFETEFQFASASH
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCC
WFETKFGNPLAFLAPEHKNKEQQIEVGKDLIAPASGKVQQDFQDNGEGIKVETSSDKIDS
EEECCCCCEEEEECCCCCCCHHHHHHCHHHCCCCCCCCHHHHHCCCCEEEEECCCCHHHH
VKEGYVVEVSKDSQTGLTVKVQHADNTYSIYGELKDVDVALYDFVDKGKKLGSIKLDDHN
CCCCEEEEECCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCC
KGVYYFAMKDGDKFIDPIQVISFE
CCEEEEEECCCCCCCCCEEEEECH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1942049; 9384377; 8459776