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The map label for this gene is spoIVFB

Identifier: 16079849

GI number: 16079849

Start: 2855973

End: 2856839

Strand: Reverse

Name: spoIVFB

Synonym: BSU27970

Alternate gene names: 16079849

Gene position: 2856839-2855973 (Counterclockwise)

Preceding gene: 16079850

Following gene: 16079848

Centisome position: 67.77

GC content: 45.44

Gene sequence:

>867_bases
TTGAATAAATGGCTCGACCTTATCTTAAAGATCCATGTGCATCCTTTTCTTTGGATTATTGCGGCGCTGGGCTTGCTCAC
AGGCCATATGAAAGCATTATTATGTCTGCTCCTGATTGTATTGATTCATGAGCTGGGGCATGCTGCTCTGGCTGTGTTTT
TTTCTTGGAGAATCAAGCGTGTTTTTTTGCTGCCGTTTGGCGGAACGGTCGAAGTGGAAGAGCACGGGAATCGGCCGTTA
AAGGAAGAGTTTGCGGTCATTATTGCCGGACCTCTTCAGCACATCTGGCTTCAGTTTGCCGCCTGGATGCTTGCAGAAGT
CTCAGTGATTCATCAGCATACCTTTGAACTCTTCACCTTTTATAATCTTTCTATCTTATTTGTCAATTTACTGCCGATCT
GGCCGCTGGATGGAGGAAAACTGTTATTTTTGTTGTTTTCCAAACAGCTGCCTTTTCAAAAGGCTCACCGGCTTAATCTA
AAAACGTCGCTCTGCTTCTGCCTGCTGCTCGGGTGCTGGGTTTTATTCGTGATTCCTCTGCAAATCAGCGCATGGGTTTT
GTTTGTCTTTCTGGCTGTTTCCTTGTTTGAGGAATATCGGCAAAGGCACTATATCCATGTGAGATTTCTCCTCGAAAGGT
ATTACGGAAAAAACAGGGAGCTTGAGAAACTTCTGCCGCTGACAGTAAAGGCGGAGGATAAAGTCTATCATGTGATGGCC
GAGTTCAAACGTGGCTGTAAGCATCCGATTATTATAGAAAAATCAGGCCAAAAGCTCAGCCAGCTTGACGAGAATGAAGT
GCTGCACGCTTACTTTGCCGATAAGCGGACGAATTCTTCCATGGAGGAACTGCTTCTGCCCTACTAA

Upstream 100 bases:

>100_bases
AAGCTCGGTTCGATTAAGCTTGATGATCATAATAAAGGGGTCTATTATTTTGCCATGAAAGACGGCGATAAATTTATTGA
TCCGATTCAGGTGATTTCAT

Downstream 100 bases:

>100_bases
AACTGATTGACAAACGCCTTGTATTTTGGTATATTTTTTAATGTTATGGATGTAGCACCATTGCTACAACCGCTCAGTAC
AGGTGTTAAGAGCTTTTACA

Product: membrane metalloprotease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MNKWLDLILKIHVHPFLWIIAALGLLTGHMKALLCLLLIVLIHELGHAALAVFFSWRIKRVFLLPFGGTVEVEEHGNRPL
KEEFAVIIAGPLQHIWLQFAAWMLAEVSVIHQHTFELFTFYNLSILFVNLLPIWPLDGGKLLFLLFSKQLPFQKAHRLNL
KTSLCFCLLLGCWVLFVIPLQISAWVLFVFLAVSLFEEYRQRHYIHVRFLLERYYGKNRELEKLLPLTVKAEDKVYHVMA
EFKRGCKHPIIIEKSGQKLSQLDENEVLHAYFADKRTNSSMEELLLPY

Sequences:

>Translated_288_residues
MNKWLDLILKIHVHPFLWIIAALGLLTGHMKALLCLLLIVLIHELGHAALAVFFSWRIKRVFLLPFGGTVEVEEHGNRPL
KEEFAVIIAGPLQHIWLQFAAWMLAEVSVIHQHTFELFTFYNLSILFVNLLPIWPLDGGKLLFLLFSKQLPFQKAHRLNL
KTSLCFCLLLGCWVLFVIPLQISAWVLFVFLAVSLFEEYRQRHYIHVRFLLERYYGKNRELEKLLPLTVKAEDKVYHVMA
EFKRGCKHPIIIEKSGQKLSQLDENEVLHAYFADKRTNSSMEELLLPY
>Mature_288_residues
MNKWLDLILKIHVHPFLWIIAALGLLTGHMKALLCLLLIVLIHELGHAALAVFFSWRIKRVFLLPFGGTVEVEEHGNRPL
KEEFAVIIAGPLQHIWLQFAAWMLAEVSVIHQHTFELFTFYNLSILFVNLLPIWPLDGGKLLFLLFSKQLPFQKAHRLNL
KTSLCFCLLLGCWVLFVIPLQISAWVLFVFLAVSLFEEYRQRHYIHVRFLLERYYGKNRELEKLLPLTVKAEDKVYHVMA
EFKRGCKHPIIIEKSGQKLSQLDENEVLHAYFADKRTNSSMEELLLPY

Specific function: Implicated in the coupling of mother cell to forespore gene expression. Required for spore formation. Processes the pro- sigma K factor

COG id: COG1994

COG function: function code R; Zn-dependent proteases

Gene ontology:

Cell location: Forespore outer membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M50B family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SP4FB_BACSU (P26937)

Other databases:

- EMBL:   X59528
- EMBL:   AL009126
- PIR:   S18438
- RefSeq:   NP_390675.1
- ProteinModelPortal:   P26937
- DIP:   DIP-48963N
- MEROPS:   M50.002
- EnsemblBacteria:   EBBACT00000000245
- GeneID:   937505
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27970
- NMPDR:   fig|224308.1.peg.2800
- GenoList:   BSU27970
- GeneTree:   EBGT00050000002642
- HOGENOM:   HBG362948
- OMA:   MIKYRDV
- ProtClustDB:   CLSK2752468
- BioCyc:   BSUB:BSU27970-MONOMER
- BRENDA:   3.4.21.116
- GO:   GO:0006508
- InterPro:   IPR008915

Pfam domain/function: PF02163 Peptidase_M50

EC number: NA

Molecular weight: Translated: 33641; Mature: 33641

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: ACT_SITE 44-44

Signals:

None

Transmembrane regions:

HASH(0xe002f8c)-; HASH(0x114ab628)-; HASH(0xd4e8764)-; HASH(0xe0030f4)-; HASH(0x112bbadc)-; HASH(0x10af139c)-;

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKWLDLILKIHVHPFLWIIAALGLLTGHMKALLCLLLIVLIHELGHAALAVFFSWRIKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VFLLPFGGTVEVEEHGNRPLKEEFAVIIAGPLQHIWLQFAAWMLAEVSVIHQHTFELFTF
EEEEECCCEEEECCCCCCCHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNLSILFVNLLPIWPLDGGKLLFLLFSKQLPFQKAHRLNLKTSLCFCLLLGCWVLFVIPL
HHHHHHHHHHHHCCCCCCCHHHHEEHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QISAWVLFVFLAVSLFEEYRQRHYIHVRFLLERYYGKNRELEKLLPLTVKAEDKVYHVMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEECCHHHHHHHH
EFKRGCKHPIIIEKSGQKLSQLDENEVLHAYFADKRTNSSMEELLLPY
HHHHCCCCCEEEECCCCHHHHCCCCHHHHHHHHCCCCCCCHHHHCCCH
>Mature Secondary Structure
MNKWLDLILKIHVHPFLWIIAALGLLTGHMKALLCLLLIVLIHELGHAALAVFFSWRIKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VFLLPFGGTVEVEEHGNRPLKEEFAVIIAGPLQHIWLQFAAWMLAEVSVIHQHTFELFTF
EEEEECCCEEEECCCCCCCHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNLSILFVNLLPIWPLDGGKLLFLLFSKQLPFQKAHRLNLKTSLCFCLLLGCWVLFVIPL
HHHHHHHHHHHHCCCCCCCHHHHEEHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QISAWVLFVFLAVSLFEEYRQRHYIHVRFLLERYYGKNRELEKLLPLTVKAEDKVYHVMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEECCHHHHHHHH
EFKRGCKHPIIIEKSGQKLSQLDENEVLHAYFADKRTNSSMEELLLPY
HHHHCCCCCEEEECCCCHHHHCCCCHHHHHHHHCCCCCCCHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1942049; 9384377; 10809718