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The map label for this gene is nifS

Identifier: 16079840

GI number: 16079840

Start: 2849569

End: 2850756

Strand: Direct

Name: nifS

Synonym: BSU27880

Alternate gene names: 16079840

Gene position: 2849569-2850756 (Clockwise)

Preceding gene: 255767637

Following gene: 255767639

Centisome position: 67.6

GC content: 43.94

Gene sequence:

>1188_bases
ATGATTTATTTAGATTATGCAGCGACAACGCCTATTTGTGAGGAAGCCCTTACTGTATATCAGAAGCTTAGTATGGATAT
GTACGGAAATGCCAGCAGCCTGCATGATGCAGGAGGAAAAGCAAAGCACATACTGGAGTACTGCCGTGAAAAAATCGCCA
ACATCATTGGCGGAGAAGCAAGCGGCATCTATTTTACAAGCGGCGGCACTGAATCCAATTTTCTGGCCATTCAATCATTG
TTAAACGGCCTGCCAAAAACGAAAAGACATTTTATTACGACTGCCATGGAGCATCAGTCCATTCATAATTGCGCTGCTTT
TTTGGAACAGCAGGGATATGATGTAACAGTCATTGAACCGAATGAATACGGGCTTATTACCGAGGAAATATTACTAACCC
ATATCCGGCCGGAAACAGGCCTTGTATCTATTCAGCATGCAAATTCTGAAACCGGGATCATTCAGCCCATTCAACACCTG
TCTTCATACCTGCACAATAAGGGGATTCTTCTTCATTGCGACGCTGTTCAAACGTTTGGAAAGATCCCGATCAATACAAA
AAATCTGGGAGTAGATGCACTCTCAATGTCCAGCCATAAAATCCACGGTCCTAAAGGAGTAGGGGCGGTCTATATCCGGC
CGGATGTCCCTTGGAAACCCGTTTATCCTCTGACTACTCACGAATACGGATTCAGAGCCGGCACCGTAAATGTTCCCGGG
ATCGGTGCCTTCACTGCCGCTGCAGAATTGATCGTAAGCGAAATGGAAAAACAGATATCCCGCAATGAGGCATTAAGAAC
ATACTTTCTAGACCAAATCCGTATTCGGTCTCTCCCTGTAACCCTTGCAGCAGACACTTCGAAAGCAGAATGTCTTCCGC
ATATTATAGGGTGTTTTTTTCACTCATTTGAAGGACAATATGTTATGTTAGAATGTAATCGGAGTAATATTTGCATTTCA
ACCGGCAGCGCTTGTTCTGCAGGTTACCACGGTCCGTCTGAAACAATGAAAGCATTAAGAAAAACCGAACAAGAAGCTCT
GCAATTTATCCGCATCTCTTTTGGCAGGCATACAACAGCTGAACAGCTGGAACAGTTATTACATACGTTTACAGTGCTTT
GGGAACAAAAGAAAGGAGAATTTGATATTGACCGAAGAATTAAAGCTAATGGGCGCCAACAGGCGTGA

Upstream 100 bases:

>100_bases
GCCATCCTCCTGTTGTTTACACCTGTCTTGACACCTATATTTACACAAGGATAAAATAAACTCAAGAGTTTTTTATGGAG
AACGGATGGAGGAAGACCGG

Downstream 100 bases:

>100_bases
CCAGCTTCTTCTGTGGCTGAAGGAATCTAAATCACCGCTGACAGGAGGAGAACTCGCAAAAAAAGCGAACGTCTCAAGAC
AGGTTATTGTACAGGATATA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MIYLDYAATTPICEEALTVYQKLSMDMYGNASSLHDAGGKAKHILEYCREKIANIIGGEASGIYFTSGGTESNFLAIQSL
LNGLPKTKRHFITTAMEHQSIHNCAAFLEQQGYDVTVIEPNEYGLITEEILLTHIRPETGLVSIQHANSETGIIQPIQHL
SSYLHNKGILLHCDAVQTFGKIPINTKNLGVDALSMSSHKIHGPKGVGAVYIRPDVPWKPVYPLTTHEYGFRAGTVNVPG
IGAFTAAAELIVSEMEKQISRNEALRTYFLDQIRIRSLPVTLAADTSKAECLPHIIGCFFHSFEGQYVMLECNRSNICIS
TGSACSAGYHGPSETMKALRKTEQEALQFIRISFGRHTTAEQLEQLLHTFTVLWEQKKGEFDIDRRIKANGRQQA

Sequences:

>Translated_395_residues
MIYLDYAATTPICEEALTVYQKLSMDMYGNASSLHDAGGKAKHILEYCREKIANIIGGEASGIYFTSGGTESNFLAIQSL
LNGLPKTKRHFITTAMEHQSIHNCAAFLEQQGYDVTVIEPNEYGLITEEILLTHIRPETGLVSIQHANSETGIIQPIQHL
SSYLHNKGILLHCDAVQTFGKIPINTKNLGVDALSMSSHKIHGPKGVGAVYIRPDVPWKPVYPLTTHEYGFRAGTVNVPG
IGAFTAAAELIVSEMEKQISRNEALRTYFLDQIRIRSLPVTLAADTSKAECLPHIIGCFFHSFEGQYVMLECNRSNICIS
TGSACSAGYHGPSETMKALRKTEQEALQFIRISFGRHTTAEQLEQLLHTFTVLWEQKKGEFDIDRRIKANGRQQA
>Mature_395_residues
MIYLDYAATTPICEEALTVYQKLSMDMYGNASSLHDAGGKAKHILEYCREKIANIIGGEASGIYFTSGGTESNFLAIQSL
LNGLPKTKRHFITTAMEHQSIHNCAAFLEQQGYDVTVIEPNEYGLITEEILLTHIRPETGLVSIQHANSETGIIQPIQHL
SSYLHNKGILLHCDAVQTFGKIPINTKNLGVDALSMSSHKIHGPKGVGAVYIRPDVPWKPVYPLTTHEYGFRAGTVNVPG
IGAFTAAAELIVSEMEKQISRNEALRTYFLDQIRIRSLPVTLAADTSKAECLPHIIGCFFHSFEGQYVMLECNRSNICIS
TGSACSAGYHGPSETMKALRKTEQEALQFIRISFGRHTTAEQLEQLLHTFTVLWEQKKGEFDIDRRIKANGRQQA

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Seems to be required for NAD biosynthesis

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=399, Percent_Identity=33.0827067669173, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI156713448, Length=409, Percent_Identity=31.7848410757946, Blast_Score=178, Evalue=9e-45,
Organism=Escherichia coli, GI48994898, Length=398, Percent_Identity=34.6733668341709, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789175, Length=297, Percent_Identity=23.9057239057239, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787970, Length=349, Percent_Identity=23.7822349570201, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25143064, Length=396, Percent_Identity=32.3232323232323, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=30.7692307692308, Blast_Score=117, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6319831, Length=408, Percent_Identity=33.8235294117647, Blast_Score=212, Evalue=8e-56,
Organism=Drosophila melanogaster, GI20129463, Length=408, Percent_Identity=32.843137254902, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_BACSU (P38033)

Other databases:

- EMBL:   M98822
- EMBL:   AL009126
- PIR:   B47071
- RefSeq:   NP_390666.1
- ProteinModelPortal:   P38033
- SMR:   P38033
- EnsemblBacteria:   EBBACT00000001846
- GeneID:   936637
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27880
- NMPDR:   fig|224308.1.peg.2791
- GenoList:   BSU27880
- GeneTree:   EBGT00050000000894
- HOGENOM:   HBG635316
- OMA:   FYRGVKS
- ProtClustDB:   PRK02948
- BioCyc:   BSUB:BSU27880-MONOMER
- BRENDA:   2.8.1.7
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 43787; Mature: 43787

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDYAATTPICEEALTVYQKLSMDMYGNASSLHDAGGKAKHILEYCREKIANIIGGEA
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCC
SGIYFTSGGTESNFLAIQSLLNGLPKTKRHFITTAMEHQSIHNCAAFLEQQGYDVTVIEP
CCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NEYGLITEEILLTHIRPETGLVSIQHANSETGIIQPIQHLSSYLHNKGILLHCDAVQTFG
CCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHC
KIPINTKNLGVDALSMSSHKIHGPKGVGAVYIRPDVPWKPVYPLTTHEYGFRAGTVNVPG
CCCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEECCCCCCC
IGAFTAAAELIVSEMEKQISRNEALRTYFLDQIRIRSLPVTLAADTSKAECLPHIIGCFF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
HSFEGQYVMLECNRSNICISTGSACSAGYHGPSETMKALRKTEQEALQFIRISFGRHTTA
HCCCCCEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EQLEQLLHTFTVLWEQKKGEFDIDRRIKANGRQQA
HHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCC
>Mature Secondary Structure
MIYLDYAATTPICEEALTVYQKLSMDMYGNASSLHDAGGKAKHILEYCREKIANIIGGEA
CEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCC
SGIYFTSGGTESNFLAIQSLLNGLPKTKRHFITTAMEHQSIHNCAAFLEQQGYDVTVIEP
CCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NEYGLITEEILLTHIRPETGLVSIQHANSETGIIQPIQHLSSYLHNKGILLHCDAVQTFG
CCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHC
KIPINTKNLGVDALSMSSHKIHGPKGVGAVYIRPDVPWKPVYPLTTHEYGFRAGTVNVPG
CCCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEECCCCCCC
IGAFTAAAELIVSEMEKQISRNEALRTYFLDQIRIRSLPVTLAADTSKAECLPHIIGCFF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
HSFEGQYVMLECNRSNICISTGSACSAGYHGPSETMKALRKTEQEALQFIRISFGRHTTA
HCCCCCEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EQLEQLLHTFTVLWEQKKGEFDIDRRIKANGRQQA
HHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377