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The map label for this gene is sigV

Identifier: 16079766

GI number: 16079766

Start: 2769850

End: 2770350

Strand: Direct

Name: sigV

Synonym: BSU27120

Alternate gene names: 16079766

Gene position: 2769850-2770350 (Clockwise)

Preceding gene: 16079765

Following gene: 16079767

Centisome position: 65.7

GC content: 37.52

Gene sequence:

>501_bases
ATGAAGAAAAAACAAACAACAAAAGCGTTGCTTGTCACATGCATAACTGACCATAAGCAAGATTTCTACAGGTTGGCTTT
CAGTTATGTGAAAAATCAAGATGACGCATTAGATATTGTTCAGGAATCTATAAAAAAAGCGCTGAGCTCAGTTGAAACGG
TCAGGAACCCTGAGACGATAAAAAGCTGGTTTTATAAAATTTTAGTACGGACAGCCATTGACTTTTTGCGCAAACAAAAA
AAGATAAGAGTGATGGACGACGAAACGATAGAATTTTTAAGCAAAGGAAAGGAAGACCATTACAAGGATACTGATCTCCA
TGAAGCGCTTGACGAATTGCCGTACCGCTATAAAACCATTATTATTTTACGTTTTTTTGAAGACCTCAAATTAGAAGAAA
TTGCGGAAATTACAGGAGAAAACACGAATACCGTCAAAACGCGCCTATACAGAGCATTGAAGCTGATGCGCATTCAGTTG
ACGAAGGAGGATCTTTCTTAA

Upstream 100 bases:

>100_bases
AAAAAATGTGATTCTGCGCAAACTATTTTTCAGCCTGCCGCGTCTATCCTAGGTAACAGCCTACGATATACGTATATTCA
AAAAGGAGCCCTTTATTGCA

Downstream 100 bases:

>100_bases
TGGATAAGAGATTACAGCAATTAAGAGAAGAATATAAAAATGTTCAGATTCCTAAAGAATTGGATATCATTGTTGAAAAA
GCCCTTCAGCAAGAACCAAA

Product: RNA polymerase ECF(extracytoplasmic function)-type sigma factor (sigma(V))

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MKKKQTTKALLVTCITDHKQDFYRLAFSYVKNQDDALDIVQESIKKALSSVETVRNPETIKSWFYKILVRTAIDFLRKQK
KIRVMDDETIEFLSKGKEDHYKDTDLHEALDELPYRYKTIIILRFFEDLKLEEIAEITGENTNTVKTRLYRALKLMRIQL
TKEDLS

Sequences:

>Translated_166_residues
MKKKQTTKALLVTCITDHKQDFYRLAFSYVKNQDDALDIVQESIKKALSSVETVRNPETIKSWFYKILVRTAIDFLRKQK
KIRVMDDETIEFLSKGKEDHYKDTDLHEALDELPYRYKTIIILRFFEDLKLEEIAEITGENTNTVKTRLYRALKLMRIQL
TKEDLS
>Mature_166_residues
MKKKQTTKALLVTCITDHKQDFYRLAFSYVKNQDDALDIVQESIKKALSSVETVRNPETIKSWFYKILVRTAIDFLRKQK
KIRVMDDETIEFLSKGKEDHYKDTDLHEALDELPYRYKTIIILRFFEDLKLEEIAEITGENTNTVKTRLYRALKLMRIQL
TKEDLS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Positively regulates the expression of proteins involved in stress responses against bacitracin, paraquat and tellurite

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): SIGV_BACSU (O05404)

Other databases:

- EMBL:   U93874
- EMBL:   AL009126
- PIR:   G69706
- RefSeq:   NP_390590.1
- ProteinModelPortal:   O05404
- SMR:   O05404
- IntAct:   O05404
- EnsemblBacteria:   EBBACT00000000940
- GeneID:   937591
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27120
- NMPDR:   fig|224308.1.peg.2715
- GenoList:   BSU27120
- GeneTree:   EBGT00070000031738
- HOGENOM:   HBG753452
- OMA:   MRIQLTK
- ProtClustDB:   CLSK887649
- BioCyc:   BSUB:BSU27120-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 19713; Mature: 19713

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKQTTKALLVTCITDHKQDFYRLAFSYVKNQDDALDIVQESIKKALSSVETVRNPETI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
KSWFYKILVRTAIDFLRKQKKIRVMDDETIEFLSKGKEDHYKDTDLHEALDELPYRYKTI
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHH
IILRFFEDLKLEEIAEITGENTNTVKTRLYRALKLMRIQLTKEDLS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKQTTKALLVTCITDHKQDFYRLAFSYVKNQDDALDIVQESIKKALSSVETVRNPETI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
KSWFYKILVRTAIDFLRKQKKIRVMDDETIEFLSKGKEDHYKDTDLHEALDELPYRYKTI
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHH
IILRFFEDLKLEEIAEITGENTNTVKTRLYRALKLMRIQLTKEDLS
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377