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The map label for this gene is yrpB

Identifier: 16079733

GI number: 16079733

Start: 2736915

End: 2737958

Strand: Direct

Name: yrpB

Synonym: BSU26800

Alternate gene names: 16079733

Gene position: 2736915-2737958 (Clockwise)

Preceding gene: 255767615

Following gene: 16079734

Centisome position: 64.92

GC content: 41.0

Gene sequence:

>1044_bases
ATGAATGAATTTATGAAAAAGTTTTCTTTAACAAAACCGATTATTCAAGCTCCAATGGCTGGCGGTATTACAAAGCCCCG
ACTTGCATCTGCAGTTTCGAATCAAGGTGCTCTTGGCAGCTTAGCATCGGGGTATCTTACGCCAGACCTCCTAGAACAAC
AAATAAAAGAAATATTTGAGCTGACAGACGCTCCTTTTCAAATTAATGTGTTTGTTCCGCTAGGTCTAGAGATGCCACCA
AAAGATCAGATTAAAAAGTGGAAAGAAAACATACCGTTAGCTAATCAAGTAAATCAATTCACATCTGTACAAGAAGAGTG
GGATGACTTCTATCAAAAAATTGATCTAATTTTAAAATACAAGGTTAAGGCTTGCTCATTCACTTTTGATCTGCCGCCTG
AAGACGCAGTAAAGGAGCTAAAAACCGCTGGATGCTGTTTAATAGGAACCGCTTCAACAGTAGAAGAAGCATTGTTAATG
GAAGAACGGGGAATGGATATAGTAGTCCTTCAAGGAAGTGAAGCCGGTGGACATCGCGGAGCATTCTTACCTTCCAAAGG
TGAATCTGCCGTAGGTTTAATGGCTCTGATTCCACAAGCAGCAGATGCACTGAGCGTACCTGTCATAGCTGCTGGGGGAA
TGATAGACCACAGAGGAGTAAAAGCAGCTTTAACCCTCGGAGCCCAAGGCGTTCAAATCGGTTCTGCCTTTTTAATTTGT
CACGAGAGTAACGCACATCCAGTGCATAAACAGAAAATACTAGAAGCAAACGAAGCAGATACAAAGCTTACGACATTATT
TTCAGGTAAAGAGGCCAGAGGAATCGTAAATAAATGGATGGAAGAAAATGAACAGTTTGAGACACAAACCCTTCCGTACC
CTTATCAAAATACACTAACGAAGGCAATGAGACAGAAGGCTTCACTTCAAAATAACCATGATCAGATGTCTTTATGGGCA
GGTCAAGGGATACGGTCATTGACTGAGGAAATTTCGGTTAAGCAGCTTTTAAATCAGCTTTGCCAAGAGGATATAAAAAT
ATAG

Upstream 100 bases:

>100_bases
AAAAAGAAGGAAAACGATATATTTAAGCGAATACGAGTTTTTGAGTAAATTTTAAAATGACACTTTTTTCAAAAATCATG
TCTTAACGGGAGGATTCGGT

Downstream 100 bases:

>100_bases
GCGCACCTCATTGATAGAGCAAAAAGCAAACTTTGACAGGCAGTTTGCTTTTTTTTTCGTAATTTCATCCACTGACTCTG
GAAATCTAAATACTCCTACA

Product: anionic nitroalkane dioxygenase

Products: NA

Alternate protein names: Nitroalkane oxidase

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNEFMKKFSLTKPIIQAPMAGGITKPRLASAVSNQGALGSLASGYLTPDLLEQQIKEIFELTDAPFQINVFVPLGLEMPP
KDQIKKWKENIPLANQVNQFTSVQEEWDDFYQKIDLILKYKVKACSFTFDLPPEDAVKELKTAGCCLIGTASTVEEALLM
EERGMDIVVLQGSEAGGHRGAFLPSKGESAVGLMALIPQAADALSVPVIAAGGMIDHRGVKAALTLGAQGVQIGSAFLIC
HESNAHPVHKQKILEANEADTKLTTLFSGKEARGIVNKWMEENEQFETQTLPYPYQNTLTKAMRQKASLQNNHDQMSLWA
GQGIRSLTEEISVKQLLNQLCQEDIKI

Sequences:

>Translated_347_residues
MNEFMKKFSLTKPIIQAPMAGGITKPRLASAVSNQGALGSLASGYLTPDLLEQQIKEIFELTDAPFQINVFVPLGLEMPP
KDQIKKWKENIPLANQVNQFTSVQEEWDDFYQKIDLILKYKVKACSFTFDLPPEDAVKELKTAGCCLIGTASTVEEALLM
EERGMDIVVLQGSEAGGHRGAFLPSKGESAVGLMALIPQAADALSVPVIAAGGMIDHRGVKAALTLGAQGVQIGSAFLIC
HESNAHPVHKQKILEANEADTKLTTLFSGKEARGIVNKWMEENEQFETQTLPYPYQNTLTKAMRQKASLQNNHDQMSLWA
GQGIRSLTEEISVKQLLNQLCQEDIKI
>Mature_347_residues
MNEFMKKFSLTKPIIQAPMAGGITKPRLASAVSNQGALGSLASGYLTPDLLEQQIKEIFELTDAPFQINVFVPLGLEMPP
KDQIKKWKENIPLANQVNQFTSVQEEWDDFYQKIDLILKYKVKACSFTFDLPPEDAVKELKTAGCCLIGTASTVEEALLM
EERGMDIVVLQGSEAGGHRGAFLPSKGESAVGLMALIPQAADALSVPVIAAGGMIDHRGVKAALTLGAQGVQIGSAFLIC
HESNAHPVHKQKILEANEADTKLTTLFSGKEARGIVNKWMEENEQFETQTLPYPYQNTLTKAMRQKASLQNNHDQMSLWA
GQGIRSLTEEISVKQLLNQLCQEDIKI

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family

Homologues:

Organism=Saccharomyces cerevisiae, GI6322609, Length=373, Percent_Identity=29.4906166219839, Blast_Score=124, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): 2NPD_BACSU (O05413)

Other databases:

- EMBL:   U93875
- EMBL:   AL009126
- PIR:   B69978
- RefSeq:   NP_390557.1
- ProteinModelPortal:   O05413
- SMR:   O05413
- EnsemblBacteria:   EBBACT00000001471
- GeneID:   936455
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU26800
- NMPDR:   fig|224308.1.peg.2682
- GenoList:   BSU26800
- GeneTree:   EBGT00050000003030
- HOGENOM:   HBG683237
- OMA:   EFIEKHE
- PhylomeDB:   O05413
- ProtClustDB:   CLSK887640
- BioCyc:   BSUB:BSU26800-MONOMER
- InterPro:   IPR004136
- InterPro:   IPR013785
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF03060 NPD

EC number: =1.13.12.16

Molecular weight: Translated: 38069; Mature: 38069

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: ACT_SITE 178-178 BINDING 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFMKKFSLTKPIIQAPMAGGITKPRLASAVSNQGALGSLASGYLTPDLLEQQIKEIFE
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHH
LTDAPFQINVFVPLGLEMPPKDQIKKWKENIPLANQVNQFTSVQEEWDDFYQKIDLILKY
HCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KVKACSFTFDLPPEDAVKELKTAGCCLIGTASTVEEALLMEERGMDIVVLQGSEAGGHRG
EEEEEEEEECCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
AFLPSKGESAVGLMALIPQAADALSVPVIAAGGMIDHRGVKAALTLGAQGVQIGSAFLIC
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCEEECCEEEEE
HESNAHPVHKQKILEANEADTKLTTLFSGKEARGIVNKWMEENEQFETQTLPYPYQNTLT
ECCCCCCHHHHHHHCCCCCCCEEHEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
KAMRQKASLQNNHDQMSLWAGQGIRSLTEEISVKQLLNQLCQEDIKI
HHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEFMKKFSLTKPIIQAPMAGGITKPRLASAVSNQGALGSLASGYLTPDLLEQQIKEIFE
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHH
LTDAPFQINVFVPLGLEMPPKDQIKKWKENIPLANQVNQFTSVQEEWDDFYQKIDLILKY
HCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KVKACSFTFDLPPEDAVKELKTAGCCLIGTASTVEEALLMEERGMDIVVLQGSEAGGHRG
EEEEEEEEECCCCHHHHHHHHHCCEEEEECHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
AFLPSKGESAVGLMALIPQAADALSVPVIAAGGMIDHRGVKAALTLGAQGVQIGSAFLIC
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCEEECCEEEEE
HESNAHPVHKQKILEANEADTKLTTLFSGKEARGIVNKWMEENEQFETQTLPYPYQNTLT
ECCCCCCHHHHHHHCCCCCCCEEHEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
KAMRQKASLQNNHDQMSLWAGQGIRSLTEEISVKQLLNQLCQEDIKI
HHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377