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The map label for this gene is grpE

Identifier: 16079602

GI number: 16079602

Start: 2627971

End: 2628534

Strand: Reverse

Name: grpE

Synonym: BSU25480

Alternate gene names: 16079602

Gene position: 2628534-2627971 (Counterclockwise)

Preceding gene: 16079603

Following gene: 16079601

Centisome position: 62.35

GC content: 43.09

Gene sequence:

>564_bases
ATGTCAGAAGAAAAACAAACCGTTGAACAAAACGAAACAGAAGAGCAAGAAATCATTGAAGAACAAGCTGCCGCTGATGA
ACAGCAGGAAGAAACAAATGAAAGCGAACTTCTTCAAAACCAAATTAACGAATTGCAAGGTTTGCTTGAGGAAAAAGAAA
ACAAACTTTTGCGTGTTCAAGCAGACTTTGAAAACTATAAACGACGCAGCCGTTTAGAGATGGAAGCGTCCCAAAAATAC
CGTTCTCAAAATATCGTGACTGATTTGCTGCCGGCTCTTGACAGTTTTGAACGAGCGCTTCAGGTTGAAGCCGACAATGA
ACAGACGAAAAGTTTGCTCCAGGGAATGGAAATGGTCCACCGTCAGCTCGTAGAAGCCTTGAAAAAAGAAGGCGTCGAAG
CCATCGAAGCTGTAGGGCAGGAATTTGATCCTAATCTGCACCAAGCTGTTATGCAGGCTGAAGACGAAAACTACGGCTCC
AACATTGTTGTTGAGGAAATGCAAAAAGGCTATAAGCTGAAGGATCGCGTCATTCGCCCTTCCATGGTCAAAGTGAATCA
ATAA

Upstream 100 bases:

>100_bases
AAGCATTAACAAGTCTGTATGATGAATAAGGGAATTTTGGCAAATTTTATCGAAGGGCAGCACCTGTCCTTCTCCTTACA
CTTTGAGGGAGGTGAACACA

Downstream 100 bases:

>100_bases
TTACATAGCAGGAGGTTATTCAAGTGAGTAAAGTTATCGGAATCGACTTAGGAACAACAAACTCATGTGTGGCAGTGCTT
GAAGGCGGCGAGCCTAAAGT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MSEEKQTVEQNETEEQEIIEEQAAADEQQEETNESELLQNQINELQGLLEEKENKLLRVQADFENYKRRSRLEMEASQKY
RSQNIVTDLLPALDSFERALQVEADNEQTKSLLQGMEMVHRQLVEALKKEGVEAIEAVGQEFDPNLHQAVMQAEDENYGS
NIVVEEMQKGYKLKDRVIRPSMVKVNQ

Sequences:

>Translated_187_residues
MSEEKQTVEQNETEEQEIIEEQAAADEQQEETNESELLQNQINELQGLLEEKENKLLRVQADFENYKRRSRLEMEASQKY
RSQNIVTDLLPALDSFERALQVEADNEQTKSLLQGMEMVHRQLVEALKKEGVEAIEAVGQEFDPNLHQAVMQAEDENYGS
NIVVEEMQKGYKLKDRVIRPSMVKVNQ
>Mature_186_residues
SEEKQTVEQNETEEQEIIEEQAAADEQQEETNESELLQNQINELQGLLEEKENKLLRVQADFENYKRRSRLEMEASQKYR
SQNIVTDLLPALDSFERALQVEADNEQTKSLLQGMEMVHRQLVEALKKEGVEAIEAVGQEFDPNLHQAVMQAEDENYGSN
IVVEEMQKGYKLKDRVIRPSMVKVNQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=175, Percent_Identity=31.4285714285714, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1788967, Length=174, Percent_Identity=34.4827586206897, Blast_Score=106, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17552458, Length=179, Percent_Identity=27.3743016759777, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324806, Length=161, Percent_Identity=30.4347826086957, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653432, Length=139, Percent_Identity=35.2517985611511, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_BACSU (P15874)

Other databases:

- EMBL:   X51477
- EMBL:   M84964
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   S08418
- RefSeq:   NP_390426.1
- PDB:   2G61
- PDBsum:   2G61
- ProteinModelPortal:   P15874
- SMR:   P15874
- EnsemblBacteria:   EBBACT00000001954
- GeneID:   937846
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25480
- NMPDR:   fig|224308.1.peg.2551
- GenoList:   BSU25480
- GeneTree:   EBGT00050000001213
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- PhylomeDB:   P15874
- ProtClustDB:   PRK14140
- BioCyc:   BSUB:BSU25480-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21683; Mature: 21552

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKQTVEQNETEEQEIIEEQAAADEQQEETNESELLQNQINELQGLLEEKENKLLRVQ
CCCHHHHHHHCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHEEEE
ADFENYKRRSRLEMEASQKYRSQNIVTDLLPALDSFERALQVEADNEQTKSLLQGMEMVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RQLVEALKKEGVEAIEAVGQEFDPNLHQAVMQAEDENYGSNIVVEEMQKGYKLKDRVIRP
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHCCC
SMVKVNQ
CHHCCCC
>Mature Secondary Structure 
SEEKQTVEQNETEEQEIIEEQAAADEQQEETNESELLQNQINELQGLLEEKENKLLRVQ
CCHHHHHHHCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHEEEE
ADFENYKRRSRLEMEASQKYRSQNIVTDLLPALDSFERALQVEADNEQTKSLLQGMEMVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RQLVEALKKEGVEAIEAVGQEFDPNLHQAVMQAEDENYGSNIVVEEMQKGYKLKDRVIRP
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHCCC
SMVKVNQ
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2108432; 1339421; 8969508; 9384377; 8012595