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The map label for this gene is comGA

Identifier: 16079529

GI number: 16079529

Start: 2559007

End: 2560077

Strand: Reverse

Name: comGA

Synonym: BSU24730

Alternate gene names: 16079529

Gene position: 2560077-2559007 (Counterclockwise)

Preceding gene: 16079530

Following gene: 16079528

Centisome position: 60.73

GC content: 41.92

Gene sequence:

>1071_bases
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGTTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Upstream 100 bases:

>100_bases
TGGTTTTTCAGCATATAACATCTCACAAAATCACGTTTTCCCTGTTTGATTACCTTTTCTTCTTTTTCTACAATATGCGT
TGAAAGGAGAGGGAATCAAA

Downstream 100 bases:

>100_bases
AAAAGTCTGGTTGTTAAAGGATCAAGCCAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGGATATACACTTCTGG
ATGGATTACGCCTGATGGAA

Product: membrane associated factor for DNA competence

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Sequences:

>Translated_356_residues
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD
>Mature_356_residues
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Specific function: Required for uptake of DNA by competent cells

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family

Homologues:

Organism=Escherichia coli, GI1789723, Length=353, Percent_Identity=36.2606232294618, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1786296, Length=271, Percent_Identity=39.4833948339483, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI87082188, Length=184, Percent_Identity=40.7608695652174, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COMGA_BACSU (P25953)

Other databases:

- EMBL:   M29691
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   B30338
- RefSeq:   NP_390353.1
- ProteinModelPortal:   P25953
- SMR:   P25953
- EnsemblBacteria:   EBBACT00000001172
- GeneID:   938516
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24730
- NMPDR:   fig|224308.1.peg.2477
- GenoList:   BSU24730
- GeneTree:   EBGT00050000002625
- HOGENOM:   HBG485518
- OMA:   FLQLQVN
- ProtClustDB:   CLSK887566
- BioCyc:   BSUB:BSU24730-MONOMER
- GO:   GO:0005622
- GO:   GO:0006810
- InterPro:   IPR003593
- InterPro:   IPR001482
- SMART:   SM00382

Pfam domain/function: PF00437 GSPII_E

EC number: NA

Molecular weight: Translated: 40459; Mature: 40459

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHF
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHH
KFLSAMDIGERRKPQNGSLTLKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLS
HHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCEEE
LFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQYAKKHFNRNIVTLEDPVETRD
ECCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
EDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
CCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCEEEEEEEC
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRAS
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEECCCCCCCHHH
VYELLYGKNLQQCIQEAKGNHANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD
HHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEECCC
>Mature Secondary Structure
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHF
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHH
KFLSAMDIGERRKPQNGSLTLKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLS
HHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCEEE
LFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQYAKKHFNRNIVTLEDPVETRD
ECCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
EDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
CCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCEEEEEEEC
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRAS
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEECCCCCCCHHH
VYELLYGKNLQQCIQEAKGNHANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD
HHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2507524; 8969508; 9384377; 9422590; 9723928