Click here to switch to the map view.

The map label for this gene is pupG

Identifier: 16079406

GI number: 16079406

Start: 2446418

End: 2447233

Strand: Reverse

Name: pupG

Synonym: BSU23490

Alternate gene names: 16079406

Gene position: 2447233-2446418 (Counterclockwise)

Preceding gene: 255767508

Following gene: 16079405

Centisome position: 58.05

GC content: 46.57

Gene sequence:

>816_bases
TTGAAGGACAGAATTGAACGCGCAGCCGCTTTTATTAAACAAAACCTGCCGGAATCTCCAAAGATCGGCCTTATTTTAGG
CTCAGGTCTTGGCATTTTGGCGGACGAAATCGAAAATCCGGTCAAGCTGAAATATGAAGATATACCTGAATTCCCGGTAT
CTACTGTTGAAGGGCATGCCGGACAGCTTGTGCTTGGCACTCTTGAAGGAGTTTCCGTCATTGCAATGCAGGGCCGCTTT
CATTTTTATGAAGGCTACTCAATGGAGAAAGTCACATTCCCTGTACGCGTGATGAAAGCGCTCGGTGTGGAAGCGTTGAT
CGTGACAAATGCCGCAGGCGGTGTCAACACTGAATTCCGTGCGGGAGATTTAATGATTATTACCGATCATATCAACTTTA
TGGGAACAAACCCGTTAATCGGGCCAAACGAAGCAGATTTCGGCGCCAGATTTCCAGATATGTCTTCAGCCTATGACAAA
GATCTGTCCAGCCTGGCTGAAAAGATTGCGAAAGACCTTAATATCCCAATTCAAAAAGGCGTGTACACTGCTGTGACAGG
ACCTTCTTACGAAACACCGGCAGAAGTCCGTTTCTTAAGAACGATGGGCTCTGATGCAGTCGGCATGTCTACTGTTCCGG
AAGTCATTGTAGCGAATCATGCGGGAATGCGGGTTCTTGGCATTTCCTGCATCTCTAACGCGGCAGCCGGAATTCTGGAT
CAGCCTTTAAGTCACGATGAAGTTATGGAAGTGACCGAAAAAGTAAAAGCTGGATTCTTAAAGCTTGTTAAAGCGATCGT
CGCTCAGTACGAATAA

Upstream 100 bases:

>100_bases
ATTTGCCGATATTGGCGCAACGATCGCTGATAATTTCCAAACAAATAAACCGAAATACGGGAAAAGCTTTTTATCTTTAT
TACAATAGGGGGACTGTTTC

Downstream 100 bases:

>100_bases
ATATGAATCAATGCAGGACCAAAAGACAGCTGTGTCTGATATCACACAGCTGTCTTTTTTTATGCCCAAAATTCGAAACA
TTTTCCATCAGTGGCGTATA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKDRIERAAAFIKQNLPESPKIGLILGSGLGILADEIENPVKLKYEDIPEFPVSTVEGHAGQLVLGTLEGVSVIAMQGRF
HFYEGYSMEKVTFPVRVMKALGVEALIVTNAAGGVNTEFRAGDLMIITDHINFMGTNPLIGPNEADFGARFPDMSSAYDK
DLSSLAEKIAKDLNIPIQKGVYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD
QPLSHDEVMEVTEKVKAGFLKLVKAIVAQYE

Sequences:

>Translated_271_residues
MKDRIERAAAFIKQNLPESPKIGLILGSGLGILADEIENPVKLKYEDIPEFPVSTVEGHAGQLVLGTLEGVSVIAMQGRF
HFYEGYSMEKVTFPVRVMKALGVEALIVTNAAGGVNTEFRAGDLMIITDHINFMGTNPLIGPNEADFGARFPDMSSAYDK
DLSSLAEKIAKDLNIPIQKGVYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD
QPLSHDEVMEVTEKVKAGFLKLVKAIVAQYE
>Mature_271_residues
MKDRIERAAAFIKQNLPESPKIGLILGSGLGILADEIENPVKLKYEDIPEFPVSTVEGHAGQLVLGTLEGVSVIAMQGRF
HFYEGYSMEKVTFPVRVMKALGVEALIVTNAAGGVNTEFRAGDLMIITDHINFMGTNPLIGPNEADFGARFPDMSSAYDK
DLSSLAEKIAKDLNIPIQKGVYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD
QPLSHDEVMEVTEKVKAGFLKLVKAIVAQYE

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family

Homologues:

Organism=Homo sapiens, GI157168362, Length=272, Percent_Identity=47.4264705882353, Blast_Score=251, Evalue=7e-67,
Organism=Homo sapiens, GI47132622, Length=247, Percent_Identity=29.1497975708502, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788746, Length=253, Percent_Identity=47.4308300395257, Blast_Score=245, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17541190, Length=276, Percent_Identity=41.6666666666667, Blast_Score=214, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6323238, Length=276, Percent_Identity=45.6521739130435, Blast_Score=217, Evalue=2e-57,
Organism=Drosophila melanogaster, GI45552885, Length=274, Percent_Identity=52.5547445255474, Blast_Score=275, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24656093, Length=274, Percent_Identity=52.5547445255474, Blast_Score=275, Evalue=2e-74,
Organism=Drosophila melanogaster, GI45552887, Length=274, Percent_Identity=52.5547445255474, Blast_Score=275, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24656090, Length=274, Percent_Identity=52.5547445255474, Blast_Score=275, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24762376, Length=239, Percent_Identity=42.2594142259414, Blast_Score=170, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUNA_BACSU (P46354)

Other databases:

- EMBL:   U32685
- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   M85047
- PIR:   D69680
- RefSeq:   NP_390230.1
- ProteinModelPortal:   P46354
- SMR:   P46354
- PhosSite:   P46354
- EnsemblBacteria:   EBBACT00000002682
- GeneID:   938731
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23490
- NMPDR:   fig|224308.1.peg.2354
- GenoList:   BSU23490
- GeneTree:   EBGT00050000002023
- HOGENOM:   HBG292914
- OMA:   VLISDHI
- PhylomeDB:   P46354
- ProtClustDB:   PRK08202
- BioCyc:   BSUB:BSU23490-MONOMER
- BRENDA:   2.4.2.1
- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099
- PANTHER:   PTHR11904
- PANTHER:   PTHR11904:SF9
- TIGRFAMs:   TIGR01700
- TIGRFAMs:   TIGR01697

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =2.4.2.1

Molecular weight: Translated: 29127; Mature: 29127

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDRIERAAAFIKQNLPESPKIGLILGSGLGILADEIENPVKLKYEDIPEFPVSTVEGHA
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
GQLVLGTLEGVSVIAMQGRFHFYEGYSMEKVTFPVRVMKALGVEALIVTNAAGGVNTEFR
CEEEEEECCCEEEEEEECCEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
AGDLMIITDHINFMGTNPLIGPNEADFGARFPDMSSAYDKDLSSLAEKIAKDLNIPIQKG
CCCEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCC
VYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD
EEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHC
QPLSHDEVMEVTEKVKAGFLKLVKAIVAQYE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDRIERAAAFIKQNLPESPKIGLILGSGLGILADEIENPVKLKYEDIPEFPVSTVEGHA
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
GQLVLGTLEGVSVIAMQGRFHFYEGYSMEKVTFPVRVMKALGVEALIVTNAAGGVNTEFR
CEEEEEECCCEEEEEEECCEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
AGDLMIITDHINFMGTNPLIGPNEADFGARFPDMSSAYDKDLSSLAEKIAKDLNIPIQKG
CCCEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCC
VYTAVTGPSYETPAEVRFLRTMGSDAVGMSTVPEVIVANHAGMRVLGISCISNAAAGILD
EEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHC
QPLSHDEVMEVTEKVKAGFLKLVKAIVAQYE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150