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The map label for this gene is dacF

Identifier: 16079405

GI number: 16079405

Start: 2445094

End: 2446263

Strand: Reverse

Name: dacF

Synonym: BSU23480

Alternate gene names: 16079405

Gene position: 2446263-2445094 (Counterclockwise)

Preceding gene: 16079406

Following gene: 16079404

Centisome position: 58.03

GC content: 41.2

Gene sequence:

>1170_bases
ATGAAACGTCTTTTATCCACTTTGTTGATTGGTATAATGCTGCTTACATTTGCACCGTCTGCATTTGCAAAACAAGACGG
AAAACGTACATCGGAGCTTGCTCATGAAGCGAAGTCTGCGGTGCTGATAGAACGTGACACGGGAAAAGTGCTTTACAACA
AGAACAGCAATGAGAGACTGGCGCCTGCAAGCATGACGAAAATTATGACGATGCTTTTGATTATGGAAGCTTTAGATAAA
GGCAAAATCAAAATGAGTGATAAGGTCCGTACAAGCGAGCATGCGGCGTCAATGGGCGGCTCACAGATATTCCTTGAGCC
CGGCGAAGAAATGACTGTCAAAGAAATGCTGAAAGGCATCGCAATCGCTTCGGGAAATGACGCTTCCGTCGCCATGGCTG
AATTTATTTCCGGCTCTGAAGAAGAATTTGTGAAGAAAATGAATAAAAAAGCAAAAGAGCTGGGATTGAAAAATACATCC
TTTAAAAACCCAACAGGACTGACCGAGGAAGGACACTACAGCTCTGCTTATGACATGGCAATCATGGCTAAGGAATTATT
GAAATACGAATCAATTACGAAGTTTACCGGCACGTATGAAGATTATCTGCGTGAAAATACAGATAAAAAGTTTTGGCTTG
TAAATACAAATCGCCTTATCAAATTTTATCCTGGTGTAGACGGCGTAAAAACAGGCTATACAGGCGAAGCGAAATATTGT
CTGACTGCTTCGGCTAAAAAAGGAAACATGCGGGCCATAGCGGTTGTATTCGGAGCGAGCACGCCTAAAGAAAGAAACGC
GCAAGTGACAAAAATGCTTGACTTCGCCTTTAGCCAATATGAAACGCATCCTTTATATAAACGAAATCAAACAGTAGCAA
AAGTAAAGGTCAAAAAAGGGAAACAAAAATTTATCGAACTCACTACATCTGAGCCGATTTCAATATTGACGAAAAAAGGC
GAGGATATGAACGATGTGAAAAAAGAAATCAAGATGAAGGACAATATTAGTGCTCCGATTCAAAAAGGCCAAGAGCTTGG
CACTCTTGTTCTGAAAAAGGATGGAGAAGTACTCGCTGAAAGTCCTGTTGCTGCAAAAGAAGATATGAAGAAAGCCGGGT
TTATCACATTCTTAAAGCGGACGATGGGAGACTGGACAAAATTTAAGTAA

Upstream 100 bases:

>100_bases
TTTTTTTATGCCCAAAATTCGAAACATTTTCCATCAGTGGCGTATAAAACCATCACGCTTGGAAAAAATAAAAAGGATTA
TGAAATGGAGGGCTTTTGAG

Downstream 100 bases:

>100_bases
TTATGCCGAATGACCACTAGTTTTGTCACGGTGAAGGAATTCATTCCGTCGAAATCGAAACACTCATTATCCGATCATAT
CAAGGAGGAATGAGCATGAG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKRLLSTLLIGIMLLTFAPSAFAKQDGKRTSELAHEAKSAVLIERDTGKVLYNKNSNERLAPASMTKIMTMLLIMEALDK
GKIKMSDKVRTSEHAASMGGSQIFLEPGEEMTVKEMLKGIAIASGNDASVAMAEFISGSEEEFVKKMNKKAKELGLKNTS
FKNPTGLTEEGHYSSAYDMAIMAKELLKYESITKFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGYTGEAKYC
LTASAKKGNMRAIAVVFGASTPKERNAQVTKMLDFAFSQYETHPLYKRNQTVAKVKVKKGKQKFIELTTSEPISILTKKG
EDMNDVKKEIKMKDNISAPIQKGQELGTLVLKKDGEVLAESPVAAKEDMKKAGFITFLKRTMGDWTKFK

Sequences:

>Translated_389_residues
MKRLLSTLLIGIMLLTFAPSAFAKQDGKRTSELAHEAKSAVLIERDTGKVLYNKNSNERLAPASMTKIMTMLLIMEALDK
GKIKMSDKVRTSEHAASMGGSQIFLEPGEEMTVKEMLKGIAIASGNDASVAMAEFISGSEEEFVKKMNKKAKELGLKNTS
FKNPTGLTEEGHYSSAYDMAIMAKELLKYESITKFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGYTGEAKYC
LTASAKKGNMRAIAVVFGASTPKERNAQVTKMLDFAFSQYETHPLYKRNQTVAKVKVKKGKQKFIELTTSEPISILTKKG
EDMNDVKKEIKMKDNISAPIQKGQELGTLVLKKDGEVLAESPVAAKEDMKKAGFITFLKRTMGDWTKFK
>Mature_389_residues
MKRLLSTLLIGIMLLTFAPSAFAKQDGKRTSELAHEAKSAVLIERDTGKVLYNKNSNERLAPASMTKIMTMLLIMEALDK
GKIKMSDKVRTSEHAASMGGSQIFLEPGEEMTVKEMLKGIAIASGNDASVAMAEFISGSEEEFVKKMNKKAKELGLKNTS
FKNPTGLTEEGHYSSAYDMAIMAKELLKYESITKFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGYTGEAKYC
LTASAKKGNMRAIAVVFGASTPKERNAQVTKMLDFAFSQYETHPLYKRNQTVAKVKVKKGKQKFIELTTSEPISILTKKG
EDMNDVKKEIKMKDNISAPIQKGQELGTLVLKKDGEVLAESPVAAKEDMKKAGFITFLKRTMGDWTKFK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family

Homologues:

Organism=Escherichia coli, GI1787062, Length=386, Percent_Identity=34.4559585492228, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1786851, Length=366, Percent_Identity=33.879781420765, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87082022, Length=394, Percent_Identity=29.6954314720812, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI87082054, Length=224, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DACF_BACSU (P38422)

Other databases:

- EMBL:   M85047
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   B42708
- RefSeq:   NP_390229.1
- ProteinModelPortal:   P38422
- SMR:   P38422
- MEROPS:   S11.005
- EnsemblBacteria:   EBBACT00000002930
- GeneID:   938732
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23480
- NMPDR:   fig|224308.1.peg.2353
- GenoList:   BSU23480
- GeneTree:   EBGT00070000031758
- HOGENOM:   HBG728991
- OMA:   EYLGGTE
- PhylomeDB:   P38422
- ProtClustDB:   CLSK873051
- BioCyc:   BSUB:BSU23480-MONOMER
- BRENDA:   3.4.16.4
- GO:   GO:0006508
- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967
- Gene3D:   G3DSA:2.60.410.10
- PRINTS:   PR00725
- SMART:   SM00936

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11; SSF56601 PBP_transp_fold; SSF69189 Peniciliin-bd_prot-assoc

EC number: =3.4.16.4

Molecular weight: Translated: 43298; Mature: 43298

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: ACT_SITE 64-64 ACT_SITE 67-67 ACT_SITE 124-124 BINDING 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLSTLLIGIMLLTFAPSAFAKQDGKRTSELAHEAKSAVLIERDTGKVLYNKNSNERL
CHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCEEEEEECCCCEEEECCCCCCC
APASMTKIMTMLLIMEALDKGKIKMSDKVRTSEHAASMGGSQIFLEPGEEMTVKEMLKGI
CHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHE
AIASGNDASVAMAEFISGSEEEFVKKMNKKAKELGLKNTSFKNPTGLTEEGHYSSAYDMA
EEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
IMAKELLKYESITKFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGYTGEAKYC
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEE
LTASAKKGNMRAIAVVFGASTPKERNAQVTKMLDFAFSQYETHPLYKRNQTVAKVKVKKG
EEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEHHHC
KQKFIELTTSEPISILTKKGEDMNDVKKEIKMKDNISAPIQKGQELGTLVLKKDGEVLAE
CCHHEEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCHHHCCHHHCEEEEECCCCHHHC
SPVAAKEDMKKAGFITFLKRTMGDWTKFK
CCCHHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MKRLLSTLLIGIMLLTFAPSAFAKQDGKRTSELAHEAKSAVLIERDTGKVLYNKNSNERL
CHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCEEEEEECCCCEEEECCCCCCC
APASMTKIMTMLLIMEALDKGKIKMSDKVRTSEHAASMGGSQIFLEPGEEMTVKEMLKGI
CHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHE
AIASGNDASVAMAEFISGSEEEFVKKMNKKAKELGLKNTSFKNPTGLTEEGHYSSAYDMA
EEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
IMAKELLKYESITKFTGTYEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGYTGEAKYC
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEE
LTASAKKGNMRAIAVVFGASTPKERNAQVTKMLDFAFSQYETHPLYKRNQTVAKVKVKKG
EEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEHHHC
KQKFIELTTSEPISILTKKGEDMNDVKKEIKMKDNISAPIQKGQELGTLVLKKDGEVLAE
CCHHEEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCHHHCCHHHCEEEEECCCCHHHC
SPVAAKEDMKKAGFITFLKRTMGDWTKFK
CCCHHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377