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The map label for this gene is sipS

Identifier: 16079388

GI number: 16079388

Start: 2432316

End: 2432870

Strand: Reverse

Name: sipS

Synonym: BSU23310

Alternate gene names: 16079388

Gene position: 2432870-2432316 (Counterclockwise)

Preceding gene: 255767503

Following gene: 16079387

Centisome position: 57.71

GC content: 41.98

Gene sequence:

>555_bases
TTGAAATCAGAAAATGTTTCGAAGAAAAAGTCAATATTAGAATGGGCAAAAGCAATTGTGATTGCTGTCGTTCTTGCTTT
GCTCATCCGCAACTTTATTTTTGCGCCGTATGTCGTTGATGGTGACTCTATGTATCCTACACTTCACAACCGTGAAAGGG
TTTTTGTTAATATGACAGTCAAATACATCGGCGAGTTTGATAGAGGAGACATCGTCGTGTTAAACGGAGATGATGTTCAC
TATGTCAAACGTATTATCGGCCTTCCCGGCGATACGGTTGAGATGAAAAATGACCAGCTCTATATCAACGGGAAAAAGGT
GGACGAACCTTATTTGGCGGCTAATAAAAAGAGAGCGAAACAGGACGGTTTTGACCATTTGACCGATGATTTCGGCCCGG
TTAAAGTGCCTGATAACAAGTATTTTGTGATGGGTGACAATCGTCGCAATTCCATGGACAGCCGTAACGGCCTTGGCCTC
TTCACGAAAAAACAAATTGCGGGTACGTCAAAGTTTGTTTTCTACCCGTTTAACGAAATGCGCAAAACAAATTAG

Upstream 100 bases:

>100_bases
AGATTAACAGTTTGTAAAATCACTTCAATGGTCCTAATGGGATTTTTTTTCGATTCGTGATAACATAAATTGAAGACCGT
TGTTTATTGGAGGAAATCAG

Downstream 100 bases:

>100_bases
GATCAAGCAGCTTCCCATTGGGGCTGCTTTTTTTATATCTTTTTTACGGTCATCCCCTAAAAACAGAACATAAATTCGTA
TATCTATAGAAAAGAAATTT

Product: type I signal peptidase

Products: NA

Alternate protein names: SPase I; Leader peptidase I

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MKSENVSKKKSILEWAKAIVIAVVLALLIRNFIFAPYVVDGDSMYPTLHNRERVFVNMTVKYIGEFDRGDIVVLNGDDVH
YVKRIIGLPGDTVEMKNDQLYINGKKVDEPYLAANKKRAKQDGFDHLTDDFGPVKVPDNKYFVMGDNRRNSMDSRNGLGL
FTKKQIAGTSKFVFYPFNEMRKTN

Sequences:

>Translated_184_residues
MKSENVSKKKSILEWAKAIVIAVVLALLIRNFIFAPYVVDGDSMYPTLHNRERVFVNMTVKYIGEFDRGDIVVLNGDDVH
YVKRIIGLPGDTVEMKNDQLYINGKKVDEPYLAANKKRAKQDGFDHLTDDFGPVKVPDNKYFVMGDNRRNSMDSRNGLGL
FTKKQIAGTSKFVFYPFNEMRKTN
>Mature_184_residues
MKSENVSKKKSILEWAKAIVIAVVLALLIRNFIFAPYVVDGDSMYPTLHNRERVFVNMTVKYIGEFDRGDIVVLNGDDVH
YVKRIIGLPGDTVEMKNDQLYINGKKVDEPYLAANKKRAKQDGFDHLTDDFGPVKVPDNKYFVMGDNRRNSMDSRNGLGL
FTKKQIAGTSKFVFYPFNEMRKTN

Specific function: Not essential for cell viability, but required for efficient secretion of many proteins

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPS_BACSU (P28628)

Other databases:

- EMBL:   Z11847
- EMBL:   L09228
- EMBL:   AL009126
- PIR:   S23381
- RefSeq:   NP_390212.1
- ProteinModelPortal:   P28628
- SMR:   P28628
- MEROPS:   S26.003
- EnsemblBacteria:   EBBACT00000001132
- GeneID:   938944
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23310
- NMPDR:   fig|224308.1.peg.2335
- GenoList:   BSU23310
- GeneTree:   EBGT00070000031959
- HOGENOM:   HBG596607
- OMA:   SKNNQIY
- ProtClustDB:   CLSK862274
- BioCyc:   BSUB:BSU23310-MONOMER
- BRENDA:   3.4.21.89
- GO:   GO:0006508
- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- Gene3D:   G3DSA:2.10.109.10
- PRINTS:   PR00727
- TIGRFAMs:   TIGR02227

Pfam domain/function: PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.89

Molecular weight: Translated: 21048; Mature: 21048

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00501 SPASE_I_1; PS00760 SPASE_I_2; PS00761 SPASE_I_3

Important sites: ACT_SITE 43-43 ACT_SITE 83-83

Signals:

None

Transmembrane regions:

HASH(0xfa8f9e8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSENVSKKKSILEWAKAIVIAVVLALLIRNFIFAPYVVDGDSMYPTLHNRERVFVNMTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCEEEEEEEE
KYIGEFDRGDIVVLNGDDVHYVKRIIGLPGDTVEMKNDQLYINGKKVDEPYLAANKKRAK
EEECCCCCCCEEEECCCCHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCHHHCCHHHHH
QDGFDHLTDDFGPVKVPDNKYFVMGDNRRNSMDSRNGLGLFTKKQIAGTSKFVFYPFNEM
HCCCHHHCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCEEEHHHHCCCCEEEEECHHHH
RKTN
CCCH
>Mature Secondary Structure
MKSENVSKKKSILEWAKAIVIAVVLALLIRNFIFAPYVVDGDSMYPTLHNRERVFVNMTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCEEEEEEEE
KYIGEFDRGDIVVLNGDDVHYVKRIIGLPGDTVEMKNDQLYINGKKVDEPYLAANKKRAK
EEECCCCCCCEEEECCCCHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCHHHCCHHHHH
QDGFDHLTDDFGPVKVPDNKYFVMGDNRRNSMDSRNGLGLFTKKQIAGTSKFVFYPFNEM
HCCCHHHCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCEEEHHHHCCCCEEEEECHHHH
RKTN
CCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1639057; 7934829; 7876097; 9384377; 8951809; 9823656