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The map label for this gene is prsW

Identifier: 16079351

GI number: 16079351

Start: 2400208

End: 2400864

Strand: Reverse

Name: prsW

Synonym: BSU22940

Alternate gene names: 16079351

Gene position: 2400864-2400208 (Counterclockwise)

Preceding gene: 255767493

Following gene: 16079350

Centisome position: 56.95

GC content: 40.64

Gene sequence:

>657_bases
ATGTTTGCAATCATCTCTGCAGGAATTGCCCCCGGCATCGCGTTATTAAGTTATTTTTATTTAAAAGATCAGTATGATAA
TGAACCTGTACATATGGTGCTACGGTCGTTTTTTCTCGGGGTTGTTCTTGTATTTCCCATCATGTTTATCCAGTATGTAC
TTGAAAAAGAGAATGTAGGAGGCGGAAGCTTTTTCGTTTCTTTTTTATCTTCGGGGTTTTTGGAGGAATCATTAAAATGG
TTTATACTGATGATCAGTGTTTACCCGCACGCCCACTTTGATGAGCATTATGACGGGATTGTGTACGGTGCAAGTGTATC
ACTCGGTTTTGCAACCCTCGAAAATATTCTTTATTTAATTGGCCACGGCGTGGAGCATGCGTTTGTCAGGGCGCTGCTGC
CTGTTTCATGCCATGCCTTGATTGGCGTTATAATGGGATTTTACCTTGGAAAAGCCCGTTTTTCCGCTGATAAGGCGCGT
GTAAAGTGGCTTACTCTTTCTTTAGTTGTCCCATCCCTTTTGCATGGATCATATGATTTTATCCTAACAGCGCTTAGCAA
TTGGATTTATTATATGCTTCCATTTATGGTATTTTTATGGTGGTTTGGTTTGCGTAAAGCGAAAAAAGCCCGTTCCGTTA
ATATGATGCAAGTATAG

Upstream 100 bases:

>100_bases
CATTCGTATGCTCTTTTTGTTTTTTTAAGTGCAGACAGATGTAAAATTTTAAACAACATGGTAGGATAGATGTAGGAATA
TAAAGGAAAGAGGTTGCCTG

Downstream 100 bases:

>100_bases
AAGCCGCACAGCAACCGTGCGGCTTTTTTGTGTGCAGTTTTTATGTTCTGAGGGATAACAAGGAAAGAGTGTATAAAAAT
AACCTCGTTACAGAAAATAC

Product: protease required for RsiW anti-sigma(W) degradation

Products: NA

Alternate protein names: Protease responsible for activating sigma-W

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MFAIISAGIAPGIALLSYFYLKDQYDNEPVHMVLRSFFLGVVLVFPIMFIQYVLEKENVGGGSFFVSFLSSGFLEESLKW
FILMISVYPHAHFDEHYDGIVYGASVSLGFATLENILYLIGHGVEHAFVRALLPVSCHALIGVIMGFYLGKARFSADKAR
VKWLTLSLVVPSLLHGSYDFILTALSNWIYYMLPFMVFLWWFGLRKAKKARSVNMMQV

Sequences:

>Translated_218_residues
MFAIISAGIAPGIALLSYFYLKDQYDNEPVHMVLRSFFLGVVLVFPIMFIQYVLEKENVGGGSFFVSFLSSGFLEESLKW
FILMISVYPHAHFDEHYDGIVYGASVSLGFATLENILYLIGHGVEHAFVRALLPVSCHALIGVIMGFYLGKARFSADKAR
VKWLTLSLVVPSLLHGSYDFILTALSNWIYYMLPFMVFLWWFGLRKAKKARSVNMMQV
>Mature_218_residues
MFAIISAGIAPGIALLSYFYLKDQYDNEPVHMVLRSFFLGVVLVFPIMFIQYVLEKENVGGGSFFVSFLSSGFLEESLKW
FILMISVYPHAHFDEHYDGIVYGASVSLGFATLENILYLIGHGVEHAFVRALLPVSCHALIGVIMGFYLGKARFSADKAR
VKWLTLSLVVPSLLHGSYDFILTALSNWIYYMLPFMVFLWWFGLRKAKKARSVNMMQV

Specific function: Is responsible for Site-1 cleavage of the RsiW anti- sigma factor. This results, after two other proteolytic steps catalyzed by the RasP and ClpXP proteases, in the release of SigW and the transcription activation of the genes under the control of the sig

COG id: COG2339

COG function: function code S; Predicted membrane protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protease prsW family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRSW_BACSU (P50738)

Other databases:

- EMBL:   L47648
- EMBL:   AL009126
- EMBL:   D79978
- PIR:   B69934
- PIR:   T44769
- RefSeq:   NP_390175.1
- ProteinModelPortal:   P50738
- EnsemblBacteria:   EBBACT00000003144
- GeneID:   938978
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22940
- NMPDR:   fig|224308.1.peg.2298
- GenoList:   BSU22940
- GeneTree:   EBGT00050000001164
- HOGENOM:   HBG549045
- OMA:   FIQYVLE
- ProtClustDB:   CLSK873083
- BioCyc:   BSUB:BSU22940-MONOMER

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24720; Mature: 24720

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10954dac)-; HASH(0x109570c8)-; HASH(0x109e7d6c)-; HASH(0x10961e84)-; HASH(0x108b9448)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAIISAGIAPGIALLSYFYLKDQYDNEPVHMVLRSFFLGVVLVFPIMFIQYVLEKENVG
CCEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGSFFVSFLSSGFLEESLKWFILMISVYPHAHFDEHYDGIVYGASVSLGFATLENILYLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHH
GHGVEHAFVRALLPVSCHALIGVIMGFYLGKARFSADKARVKWLTLSLVVPSLLHGSYDF
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHH
ILTALSNWIYYMLPFMVFLWWFGLRKAKKARSVNMMQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MFAIISAGIAPGIALLSYFYLKDQYDNEPVHMVLRSFFLGVVLVFPIMFIQYVLEKENVG
CCEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGSFFVSFLSSGFLEESLKWFILMISVYPHAHFDEHYDGIVYGASVSLGFATLENILYLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHH
GHGVEHAFVRALLPVSCHALIGVIMGFYLGKARFSADKARVKWLTLSLVVPSLLHGSYDF
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHH
ILTALSNWIYYMLPFMVFLWWFGLRKAKKARSVNMMQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 8830707