Click here to switch to the map view.

The map label for this gene is sleB

Identifier: 16079350

GI number: 16079350

Start: 2399152

End: 2400069

Strand: Reverse

Name: sleB

Synonym: BSU22930

Alternate gene names: 16079350

Gene position: 2400069-2399152 (Counterclockwise)

Preceding gene: 16079351

Following gene: 16079349

Centisome position: 56.93

GC content: 45.21

Gene sequence:

>918_bases
ATGAAGTCCAAAGGATCGATTATGGCATGTCTCATCCTTTTTTCCTTTACAATAACGACGTTTATTAATACTGAAACGAT
CTCTGCCTTTTCGAATCAGGTCATTCAAAGAGGGGCAACAGGGGATGATGTGGTCGAGCTTCAGGCGCGTCTTCAATACA
ACGGATATTATAACGGAAAAATTGACGGGGTTTATGGATGGGGGACGTACTGGGCAGTTCGAAATTTTCAGGATCAATTC
GGGTTAAAAGAGGTTGACGGCCTTGTAGGAGCTAAAACAAAGCAAACCTTAATATGTAAATCAAAATACTATCGTGAATA
TGTCATGGAACAGCTCAATAAAGGGAATACATTCACGCATTACGGAAAAATTCCGCTAAAGTATCAGACGAAACCATCAA
AAGCAGCAACACAAAAGGCAAGACAACAAGCAGAAGCACGGCAGAAACAGCCTGCGGAAAAAACAACGCAGAAGCCTAAA
GCGAATGCGAATAAACAGCAAAACAATACACCAGCAAAAGCAAGAAAACAGGATGCGGTAGCAGCGAACATGCCTGGTGG
ATTTTCCAACAACGATATCAGGCTGCTTGCTCAAGCGGTTTATGGCGAAGCCCGGGGCGAGCCGTACGAGGGGCAGGTTG
CTATTGCAGCAGTCATTTTAAACCGTTTGAACAGCCCGTTATTTCCAAATTCAGTAGCGGGGGTTATTTTTGAGCCGCTT
GCCTTCACAGCAGTAGCCGACGGACAAATTTACATGCAGCCGAATGAAACGGCACGAGAAGCAGTGCTGGATGCCATCAA
TGGCTGGGACCCATCAGAGGAAGCACTTTACTACTTTAATCCGGATACGGCTACAAGTCCGTGGATTTGGGGGCGTCCGC
AGATTAAAAGAATCGGTAAACACATTTTCTGTGAGTAG

Upstream 100 bases:

>100_bases
TTTTATGTTCTGAGGGATAACAAGGAAAGAGTGTATAAAAATAACCTCGTTACAGAAAATACGATTACACTTAAAAATTT
GCAGTAGGAGGCTTGAAAAG

Downstream 100 bases:

>100_bases
CAGATATGAGAAAGCATAAAAAGAGGTGTGATAAATGATCAGAGGAATTTTAATCGCCGTGCTTGGTATTGCAATAGTCG
GTACAGGCTACTGGGGATAC

Product: spore cortex-lytic enzyme

Products: NA

Alternate protein names: SCLE

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKSKGSIMACLILFSFTITTFINTETISAFSNQVIQRGATGDDVVELQARLQYNGYYNGKIDGVYGWGTYWAVRNFQDQF
GLKEVDGLVGAKTKQTLICKSKYYREYVMEQLNKGNTFTHYGKIPLKYQTKPSKAATQKARQQAEARQKQPAEKTTQKPK
ANANKQQNNTPAKARKQDAVAANMPGGFSNNDIRLLAQAVYGEARGEPYEGQVAIAAVILNRLNSPLFPNSVAGVIFEPL
AFTAVADGQIYMQPNETAREAVLDAINGWDPSEEALYYFNPDTATSPWIWGRPQIKRIGKHIFCE

Sequences:

>Translated_305_residues
MKSKGSIMACLILFSFTITTFINTETISAFSNQVIQRGATGDDVVELQARLQYNGYYNGKIDGVYGWGTYWAVRNFQDQF
GLKEVDGLVGAKTKQTLICKSKYYREYVMEQLNKGNTFTHYGKIPLKYQTKPSKAATQKARQQAEARQKQPAEKTTQKPK
ANANKQQNNTPAKARKQDAVAANMPGGFSNNDIRLLAQAVYGEARGEPYEGQVAIAAVILNRLNSPLFPNSVAGVIFEPL
AFTAVADGQIYMQPNETAREAVLDAINGWDPSEEALYYFNPDTATSPWIWGRPQIKRIGKHIFCE
>Mature_305_residues
MKSKGSIMACLILFSFTITTFINTETISAFSNQVIQRGATGDDVVELQARLQYNGYYNGKIDGVYGWGTYWAVRNFQDQF
GLKEVDGLVGAKTKQTLICKSKYYREYVMEQLNKGNTFTHYGKIPLKYQTKPSKAATQKARQQAEARQKQPAEKTTQKPK
ANANKQQNNTPAKARKQDAVAANMPGGFSNNDIRLLAQAVYGEARGEPYEGQVAIAAVILNRLNSPLFPNSVAGVIFEPL
AFTAVADGQIYMQPNETAREAVLDAINGWDPSEEALYYFNPDTATSPWIWGRPQIKRIGKHIFCE

Specific function: Could be a lytic transglycosylase. Required for spore cortex hydrolysis during germination. Interacts strongly but noncovalently with spore components

COG id: COG3409

COG function: function code M; Putative peptidoglycan-binding domain-containing protein

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SLEB_BACSU (P50739)

Other databases:

- EMBL:   D79978
- EMBL:   L47648
- EMBL:   AL009126
- PIR:   C69708
- RefSeq:   NP_390174.1
- ProteinModelPortal:   P50739
- SMR:   P50739
- EnsemblBacteria:   EBBACT00000003874
- GeneID:   938979
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22930
- NMPDR:   fig|224308.1.peg.2297
- GenoList:   BSU22930
- GeneTree:   EBGT00050000000251
- HOGENOM:   HBG498645
- OMA:   YNGKIDG
- ProtClustDB:   CLSK887503
- BioCyc:   BSUB:BSU22930-MONOMER
- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224
- Gene3D:   G3DSA:1.10.101.10
- TIGRFAMs:   TIGR02869

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1; SSF47090 PGBD_like

EC number: 3.5.1.28

Molecular weight: Translated: 34002; Mature: 34002

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKGSIMACLILFSFTITTFINTETISAFSNQVIQRGATGDDVVELQARLQYNGYYNGK
CCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCEECCE
IDGVYGWGTYWAVRNFQDQFGLKEVDGLVGAKTKQTLICKSKYYREYVMEQLNKGNTFTH
ECCEECCHHHHHHHCHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
YGKIPLKYQTKPSKAATQKARQQAEARQKQPAEKTTQKPKANANKQQNNTPAKARKQDAV
ECCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCE
AANMPGGFSNNDIRLLAQAVYGEARGEPYEGQVAIAAVILNRLNSPLFPNSVAGVIFEPL
EECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AFTAVADGQIYMQPNETAREAVLDAINGWDPSEEALYYFNPDTATSPWIWGRPQIKRIGK
HHEEEECCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEECCCHHHHHHHH
HIFCE
HHCCC
>Mature Secondary Structure
MKSKGSIMACLILFSFTITTFINTETISAFSNQVIQRGATGDDVVELQARLQYNGYYNGK
CCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCEECCE
IDGVYGWGTYWAVRNFQDQFGLKEVDGLVGAKTKQTLICKSKYYREYVMEQLNKGNTFTH
ECCEECCHHHHHHHCHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
YGKIPLKYQTKPSKAATQKARQQAEARQKQPAEKTTQKPKANANKQQNNTPAKARKQDAV
ECCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCE
AANMPGGFSNNDIRLLAQAVYGEARGEPYEGQVAIAAVILNRLNSPLFPNSVAGVIFEPL
EECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AFTAVADGQIYMQPNETAREAVLDAINGWDPSEEALYYFNPDTATSPWIWGRPQIKRIGK
HHEEEECCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEECCCHHHHHHHH
HIFCE
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8830707; 8760912; 9384377; 10197998; 10658652