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The map label for this gene is trpE

Identifier: 16079325

GI number: 16079325

Start: 2375869

End: 2377416

Strand: Reverse

Name: trpE

Synonym: BSU22680

Alternate gene names: 16079325

Gene position: 2377416-2375869 (Counterclockwise)

Preceding gene: 16079326

Following gene: 50812260

Centisome position: 56.4

GC content: 42.96

Gene sequence:

>1548_bases
ATGAATTTCCAATCAAACATTTCCGCATTTTTAGAGGACAGCTTGTCCCACCACACGATACCGATTGTGGAGACCTTCAC
AGTCGATACACTGACACCCATTCAAATGATAGAGAAGCTTGACAGGGAGATTACGTATCTTCTTGAAAGCAAGGACGATA
CATCCACTTGGTCCAGATATTCGTTTATCGGCCTGAATCCATTTCTCACAATTAAAGAAGAGCAGGGCCGTTTTTCGGCC
GCTGATCAGGACAGCAAATCTCTTTACACAGGAAATGAACTAAAAGAAGTGCTGAACTGGATGAATACCACATACAAAAT
CAAAACACCTGAGCTTGGCATTCCTTTTGTCGGCGGAGCTGTCGGGTACTTAAGCTATGATATGATCCCGCTGATTGAGC
CTTCTGTTCCTTCGCATACCAAAGAAACAGACATGGAAAAGTGTATGCTGTTTGTTTGCCGGACATTAATTGCGTATGAT
CATGAAACCAAAAACGTCCACTTTATCCAATATGCAAGGCTCACTGGAGAGGAAACAAAAAACGAAAAAATGGATGTATT
CCATCAAAATCATCTGGAGCTTCAAAATCTCATTGAAAAAATGATGGACCAAAAAAACATAAAAGAGCTGTTTCTTTCTG
CTGATTCATACAAGACACCCAGCTTTGAGACAGTATCTTCTAATTATGAAAAATCGGCTTTTATGGCTGATGTAGAAAAA
ATCAAAAGCTATATAAAAGCAGGCGATATCTTCCAGGGTGTTTTATCACAAAAATTTGAGGTGCCGATAAAAGCAGATGC
TTTTGAGTTATACCGAGTGCTTAGGATCGTCAATCCTTCGCCGTATATGTATTATATGAAACTGCTAGACAGAGAAATAG
TCGGCAGCTCTCCGGAACGGTTAATACACGTTCAAGACGGGCACTTAGAAATCCATCCGATTGCCGGTACGAGAAAACGC
GGTGCAGACAAAGCTGAAGATGAGAGACTGAAGGTTGAGCTCATGAAGGATGAAAAAGAAAAAGCGGAGCATTACATGCT
CGTTGATCTTGCCCGAAACGATATCGGCAGAGTAGCAGAGTATGGTTCTGTTTCTGTGCCGGAGTTCACAAAAATTGTTT
CCTTTTCACATGTCATGCACATTATCTCGGTGGTTACAGGCCGATTGAAAAAAGGGGTTCATCCTGTCGATGCACTGATG
TCTGCTTTCCCGGCGGGGACTTTAACAGGCGCACCCAAAATCCGTGCCATGCAGCTTTTGCAAGAACTCGAGCCAACACC
GAGAGAGACATACGGAGGGTGTATTGCCTACATTGGGTTTGACGGGAATATCGACTCTTGTATTACGATTCGCACGATGA
GTGTAAAGAACGGTGTTGCATCGATACAGGCAGGTGCTGGCATTGTTGCTGATTCTGTTCCGGAAGCCGAATACGAAGAA
AGCTGTAATAAAGCCGGTGCGCTGCTGAAAACGATTCATATTGCAGAAGACATGTTTCATAGCAAGGAGGATAAAGCTGA
TGAACAGATTTCTACAATTGTGCGTTGA

Upstream 100 bases:

>100_bases
TTCTACCCAAAAGAAGTCTTTCTTTTGGGTTTATTTGTTATATAGTATTTTATCCTCTCATGCCATCTTCTCATTCTCCT
TGCCATAAGGAGTGAGAGCA

Downstream 100 bases:

>100_bases
CGGAAAAACCCTTACTGCCGGTGAGGCTGAAACGCTGATGAATATGATGATGGCAGCGGAAATGACTCCTTCTGAAATGG
GGGGGATATTGTCAATTCTT

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MNFQSNISAFLEDSLSHHTIPIVETFTVDTLTPIQMIEKLDREITYLLESKDDTSTWSRYSFIGLNPFLTIKEEQGRFSA
ADQDSKSLYTGNELKEVLNWMNTTYKIKTPELGIPFVGGAVGYLSYDMIPLIEPSVPSHTKETDMEKCMLFVCRTLIAYD
HETKNVHFIQYARLTGEETKNEKMDVFHQNHLELQNLIEKMMDQKNIKELFLSADSYKTPSFETVSSNYEKSAFMADVEK
IKSYIKAGDIFQGVLSQKFEVPIKADAFELYRVLRIVNPSPYMYYMKLLDREIVGSSPERLIHVQDGHLEIHPIAGTRKR
GADKAEDERLKVELMKDEKEKAEHYMLVDLARNDIGRVAEYGSVSVPEFTKIVSFSHVMHIISVVTGRLKKGVHPVDALM
SAFPAGTLTGAPKIRAMQLLQELEPTPRETYGGCIAYIGFDGNIDSCITIRTMSVKNGVASIQAGAGIVADSVPEAEYEE
SCNKAGALLKTIHIAEDMFHSKEDKADEQISTIVR

Sequences:

>Translated_515_residues
MNFQSNISAFLEDSLSHHTIPIVETFTVDTLTPIQMIEKLDREITYLLESKDDTSTWSRYSFIGLNPFLTIKEEQGRFSA
ADQDSKSLYTGNELKEVLNWMNTTYKIKTPELGIPFVGGAVGYLSYDMIPLIEPSVPSHTKETDMEKCMLFVCRTLIAYD
HETKNVHFIQYARLTGEETKNEKMDVFHQNHLELQNLIEKMMDQKNIKELFLSADSYKTPSFETVSSNYEKSAFMADVEK
IKSYIKAGDIFQGVLSQKFEVPIKADAFELYRVLRIVNPSPYMYYMKLLDREIVGSSPERLIHVQDGHLEIHPIAGTRKR
GADKAEDERLKVELMKDEKEKAEHYMLVDLARNDIGRVAEYGSVSVPEFTKIVSFSHVMHIISVVTGRLKKGVHPVDALM
SAFPAGTLTGAPKIRAMQLLQELEPTPRETYGGCIAYIGFDGNIDSCITIRTMSVKNGVASIQAGAGIVADSVPEAEYEE
SCNKAGALLKTIHIAEDMFHSKEDKADEQISTIVR
>Mature_515_residues
MNFQSNISAFLEDSLSHHTIPIVETFTVDTLTPIQMIEKLDREITYLLESKDDTSTWSRYSFIGLNPFLTIKEEQGRFSA
ADQDSKSLYTGNELKEVLNWMNTTYKIKTPELGIPFVGGAVGYLSYDMIPLIEPSVPSHTKETDMEKCMLFVCRTLIAYD
HETKNVHFIQYARLTGEETKNEKMDVFHQNHLELQNLIEKMMDQKNIKELFLSADSYKTPSFETVSSNYEKSAFMADVEK
IKSYIKAGDIFQGVLSQKFEVPIKADAFELYRVLRIVNPSPYMYYMKLLDREIVGSSPERLIHVQDGHLEIHPIAGTRKR
GADKAEDERLKVELMKDEKEKAEHYMLVDLARNDIGRVAEYGSVSVPEFTKIVSFSHVMHIISVVTGRLKKGVHPVDALM
SAFPAGTLTGAPKIRAMQLLQELEPTPRETYGGCIAYIGFDGNIDSCITIRTMSVKNGVASIQAGAGIVADSVPEAEYEE
SCNKAGALLKTIHIAEDMFHSKEDKADEQISTIVR

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family

Homologues:

Organism=Escherichia coli, GI1787518, Length=432, Percent_Identity=34.4907407407407, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1788114, Length=447, Percent_Identity=30.6487695749441, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1786809, Length=268, Percent_Identity=26.1194029850746, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082077, Length=216, Percent_Identity=29.1666666666667, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320935, Length=471, Percent_Identity=38.6411889596603, Blast_Score=283, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6324361, Length=402, Percent_Identity=26.3681592039801, Blast_Score=116, Evalue=7e-27,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPE_BACSU (P03963)

Other databases:

- EMBL:   K01391
- EMBL:   M80245
- EMBL:   AL009126
- EMBL:   M27566
- PIR:   A01119
- RefSeq:   NP_390149.1
- ProteinModelPortal:   P03963
- SMR:   P03963
- EnsemblBacteria:   EBBACT00000001242
- GeneID:   939008
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22680
- NMPDR:   fig|224308.1.peg.2272
- GenoList:   BSU22680
- GeneTree:   EBGT00050000001425
- HOGENOM:   HBG507440
- OMA:   NGVASIQ
- PhylomeDB:   P03963
- ProtClustDB:   PRK13569
- BioCyc:   BSUB:BSU22680-MONOMER
- BRENDA:   4.1.3.27
- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236
- PRINTS:   PR00095
- TIGRFAMs:   TIGR00564

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =4.1.3.27

Molecular weight: Translated: 58117; Mature: 58117

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQSNISAFLEDSLSHHTIPIVETFTVDTLTPIQMIEKLDREITYLLESKDDTSTWSRY
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHEE
SFIGLNPFLTIKEEQGRFSAADQDSKSLYTGNELKEVLNWMNTTYKIKTPELGIPFVGGA
EEEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCH
VGYLSYDMIPLIEPSVPSHTKETDMEKCMLFVCRTLIAYDHETKNVHFIQYARLTGEETK
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCC
NEKMDVFHQNHLELQNLIEKMMDQKNIKELFLSADSYKTPSFETVSSNYEKSAFMADVEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHH
IKSYIKAGDIFQGVLSQKFEVPIKADAFELYRVLRIVNPSPYMYYMKLLDREIVGSSPER
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
LIHVQDGHLEIHPIAGTRKRGADKAEDERLKVELMKDEKEKAEHYMLVDLARNDIGRVAE
EEEEECCCEEEEECCCCCCCCCCCCCCCCEEHHHHHCHHHHHHHEEEEEHHHHHHHHHHH
YGSVSVPEFTKIVSFSHVMHIISVVTGRLKKGVHPVDALMSAFPAGTLTGAPKIRAMQLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHH
QELEPTPRETYGGCIAYIGFDGNIDSCITIRTMSVKNGVASIQAGAGIVADSVPEAEYEE
HHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEHHCCHHHHHCCCCCCCCCCCCHHHHH
SCNKAGALLKTIHIAEDMFHSKEDKADEQISTIVR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNFQSNISAFLEDSLSHHTIPIVETFTVDTLTPIQMIEKLDREITYLLESKDDTSTWSRY
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHEE
SFIGLNPFLTIKEEQGRFSAADQDSKSLYTGNELKEVLNWMNTTYKIKTPELGIPFVGGA
EEEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCH
VGYLSYDMIPLIEPSVPSHTKETDMEKCMLFVCRTLIAYDHETKNVHFIQYARLTGEETK
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCC
NEKMDVFHQNHLELQNLIEKMMDQKNIKELFLSADSYKTPSFETVSSNYEKSAFMADVEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHH
IKSYIKAGDIFQGVLSQKFEVPIKADAFELYRVLRIVNPSPYMYYMKLLDREIVGSSPER
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
LIHVQDGHLEIHPIAGTRKRGADKAEDERLKVELMKDEKEKAEHYMLVDLARNDIGRVAE
EEEEECCCEEEEECCCCCCCCCCCCCCCCEEHHHHHCHHHHHHHEEEEEHHHHHHHHHHH
YGSVSVPEFTKIVSFSHVMHIISVVTGRLKKGVHPVDALMSAFPAGTLTGAPKIRAMQLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHH
QELEPTPRETYGGCIAYIGFDGNIDSCITIRTMSVKNGVASIQAGAGIVADSVPEAEYEE
HHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEHHCCHHHHHCCCCCCCCCCCCHHHHH
SCNKAGALLKTIHIAEDMFHSKEDKADEQISTIVR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3924737; 6425119; 9384377; 6436812; 2422155