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The map label for this gene is nth

Identifier: 16079291

GI number: 16079291

Start: 2344755

End: 2345414

Strand: Reverse

Name: nth

Synonym: BSU22340

Alternate gene names: 16079291

Gene position: 2345414-2344755 (Counterclockwise)

Preceding gene: 16079292

Following gene: 16079290

Centisome position: 55.64

GC content: 45.45

Gene sequence:

>660_bases
GTGTTAAATCTAAAACAAATTGAATTCTGTTTAGACAAGATAGGTGACATGTTTCCTCATGCGGAGTGTGAACTGGTTCA
TTCCAATCCTTTTGAATTAGTGGTGGCTGTTGCTTTATCTGCGCAATGTACAGATGCACTTGTAAACAGAGTGACCAAAA
CATTATTCCAAAAATATAAACGGCCGGAAGACTATTTGGCTGTTCCGCTGGAAGAGCTTCAGCAGGATATTAAATCAATC
GGTTTATATCGTAATAAAGCGAAAAATATTCAAAAGCTGAGTAAAATGATTATTGAAGATTACGGCGGAGAAGTGCCGAG
AGACCGCGATGAGCTTGTCAAACTGCCAGGGGTCGGGAGAAAGACCGCAAACGTAGTGGTATCCGTTGCGTTTGGCGTGC
CGGCCATAGCCGTAGATACCCATGTGGAGAGAGTCAGCAAACGATTGGGCATTTGCCGGTGGAAGGACTCGGTTCTGGAA
GTTGAAAAGACGCTGATGCGCAAGGTTCCCAAAGAAGATTGGTCCGTTACGCATCACCGGCTTATTTTCTTCGGCAGATA
TCACTGTAAAGCCCAATCTCCGCGCTGTGCGGAGTGTCCGCTGCTTTCTTTGTGCAGAGAAGGGCAGAAGAGGGATAAAA
AAGGACTGGTGAAACGATGA

Upstream 100 bases:

>100_bases
CCGGCGTGTACAAGCAAAGCAGAATGAACCGCAAAAAGAGTATAAAAGGCAGGTTCCTTTTTACAATTGGCTTGAACAAT
AAAGTGAAAAGGTGACAATC

Downstream 100 bases:

>100_bases
CGCAAGCAAAAGAAGTGTTGGCTTCCTATGAGCAATACTTGCGCAGTCTTGGCCAGAAGAGTTCCTCGGATATGAAGAAA
ACCTTGCAGACCAATCCCGT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MLNLKQIEFCLDKIGDMFPHAECELVHSNPFELVVAVALSAQCTDALVNRVTKTLFQKYKRPEDYLAVPLEELQQDIKSI
GLYRNKAKNIQKLSKMIIEDYGGEVPRDRDELVKLPGVGRKTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLE
VEKTLMRKVPKEDWSVTHHRLIFFGRYHCKAQSPRCAECPLLSLCREGQKRDKKGLVKR

Sequences:

>Translated_219_residues
MLNLKQIEFCLDKIGDMFPHAECELVHSNPFELVVAVALSAQCTDALVNRVTKTLFQKYKRPEDYLAVPLEELQQDIKSI
GLYRNKAKNIQKLSKMIIEDYGGEVPRDRDELVKLPGVGRKTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLE
VEKTLMRKVPKEDWSVTHHRLIFFGRYHCKAQSPRCAECPLLSLCREGQKRDKKGLVKR
>Mature_219_residues
MLNLKQIEFCLDKIGDMFPHAECELVHSNPFELVVAVALSAQCTDALVNRVTKTLFQKYKRPEDYLAVPLEELQQDIKSI
GLYRNKAKNIQKLSKMIIEDYGGEVPRDRDELVKLPGVGRKTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLE
VEKTLMRKVPKEDWSVTHHRLIFFGRYHCKAQSPRCAECPLLSLCREGQKRDKKGLVKR

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family

Homologues:

Organism=Homo sapiens, GI4505471, Length=184, Percent_Identity=33.1521739130435, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI190358497, Length=200, Percent_Identity=28, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI115298648, Length=200, Percent_Identity=28, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI6912520, Length=200, Percent_Identity=28, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI115298650, Length=200, Percent_Identity=28, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI115298654, Length=200, Percent_Identity=28, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI115298652, Length=200, Percent_Identity=28, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1787920, Length=212, Percent_Identity=45.7547169811321, Blast_Score=173, Evalue=7e-45,
Organism=Escherichia coli, GI1789331, Length=185, Percent_Identity=24.8648648648649, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=36.8715083798883, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=191, Percent_Identity=25.130890052356, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319304, Length=178, Percent_Identity=26.9662921348315, Blast_Score=62, Evalue=8e-11,
Organism=Drosophila melanogaster, GI45550361, Length=195, Percent_Identity=31.2820512820513, Blast_Score=97, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END3_BACSU (P39788)

Other databases:

- EMBL:   U11289
- EMBL:   L47709
- EMBL:   AL009126
- PIR:   I40525
- RefSeq:   NP_390115.1
- ProteinModelPortal:   P39788
- SMR:   P39788
- EnsemblBacteria:   EBBACT00000001693
- GeneID:   939036
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22340
- NMPDR:   fig|224308.1.peg.2238
- GenoList:   BSU22340
- GeneTree:   EBGT00050000000495
- HOGENOM:   HBG464473
- OMA:   FGEPTIA
- PhylomeDB:   P39788
- ProtClustDB:   CLSK2518244
- BioCyc:   BSUB:BSU22340-MONOMER
- BRENDA:   4.2.99.18
- GO:   GO:0005622
- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- Gene3D:   G3DSA:1.10.340.30
- Gene3D:   G3DSA:1.10.1670.10
- SMART:   SM00478
- SMART:   SM00525
- SMART:   SM00278
- TIGRFAMs:   TIGR01083

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD; SSF48150 DNA_glycsylse

EC number: =4.2.99.18

Molecular weight: Translated: 25001; Mature: 25001

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00764 ENDONUCLEASE_III_1; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLKQIEFCLDKIGDMFPHAECELVHSNPFELVVAVALSAQCTDALVNRVTKTLFQKYK
CCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPEDYLAVPLEELQQDIKSIGLYRNKAKNIQKLSKMIIEDYGGEVPRDRDELVKLPGVGR
CCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC
KTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLEVEKTLMRKVPKEDWSVTHHR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LIFFGRYHCKAQSPRCAECPLLSLCREGQKRDKKGLVKR
HEEEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLNLKQIEFCLDKIGDMFPHAECELVHSNPFELVVAVALSAQCTDALVNRVTKTLFQKYK
CCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPEDYLAVPLEELQQDIKSIGLYRNKAKNIQKLSKMIIEDYGGEVPRDRDELVKLPGVGR
CCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCC
KTANVVVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLEVEKTLMRKVPKEDWSVTHHR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LIFFGRYHCKAQSPRCAECPLLSLCREGQKRDKKGLVKR
HEEEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377