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The map label for this gene is ypqE

Identifier: 16079281

GI number: 16079281

Start: 2336933

End: 2337439

Strand: Reverse

Name: ypqE

Synonym: BSU22230

Alternate gene names: 16079281

Gene position: 2337439-2336933 (Counterclockwise)

Preceding gene: 16079283

Following gene: 16079280

Centisome position: 55.45

GC content: 44.18

Gene sequence:

>507_bases
TTGCTGAAAAAATTATTCGGAATGGGCAAAATTCAGGAAAAGGTGACAGAAGAGGTTATTTATTCTCCGGCAGACGGTAC
AGTAATGGACCTGTCAGATGTACCGGACCCTGTTTTTTCACAGAAAATGATGGGAGAAGGAATAGCGGTGGAACCTTCTA
GCGGTGAAATCGTTTCTCCCGCTGAAGGTGAAGTCATTCAGATTTTCCACACCAAACACGCCGTTGGCATTCGGACTCGC
TCGGGAATTGAGCTGTTAATTCATGTCGGGCTGGAGACAGTCAATATGAACGGAGAAGGATTTACTGCCCACATTAAAGA
AGGGGATAAAGTGAAGGTCGGTGACCCGCTGATTACATGTGATTTGGAATTAATAAAAGAAAAAGCGAGCAGCACAGTCA
TCCCAATCGTTATAATGAATGGTGAGGCTGTAGGATCAATGGTATCGGCAGGCGAAAAGGCTGCTCGTAAAGGCGAATCA
AAGCTCTTTACAATAAAAGCGAAATAA

Upstream 100 bases:

>100_bases
TGCCAGATGTTGTTATAAATTGAAGCAAAATTGAAAGTGTGGTTAAATAGTGGTGAATGAAGGGATAACGGTTGAAGTGA
ATGAGAAGGGAGAAAATACA

Downstream 100 bases:

>100_bases
GCAGGGCGTATGCCTTGCTTTTTTTATTCAACTGTATAAAATTTGCACCTAGCCATGCAAACAAATATTCGGTAAACTGT
ACTTGACAAAGAGGTGTAAA

Product: phosphotransferase system enzyme IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MLKKLFGMGKIQEKVTEEVIYSPADGTVMDLSDVPDPVFSQKMMGEGIAVEPSSGEIVSPAEGEVIQIFHTKHAVGIRTR
SGIELLIHVGLETVNMNGEGFTAHIKEGDKVKVGDPLITCDLELIKEKASSTVIPIVIMNGEAVGSMVSAGEKAARKGES
KLFTIKAK

Sequences:

>Translated_168_residues
MLKKLFGMGKIQEKVTEEVIYSPADGTVMDLSDVPDPVFSQKMMGEGIAVEPSSGEIVSPAEGEVIQIFHTKHAVGIRTR
SGIELLIHVGLETVNMNGEGFTAHIKEGDKVKVGDPLITCDLELIKEKASSTVIPIVIMNGEAVGSMVSAGEKAARKGES
KLFTIKAK
>Mature_168_residues
MLKKLFGMGKIQEKVTEEVIYSPADGTVMDLSDVPDPVFSQKMMGEGIAVEPSSGEIVSPAEGEVIQIFHTKHAVGIRTR
SGIELLIHVGLETVNMNGEGFTAHIKEGDKVKVGDPLITCDLELIKEKASSTVIPIVIMNGEAVGSMVSAGEKAARKGES
KLFTIKAK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain

Homologues:

Organism=Escherichia coli, GI1788757, Length=129, Percent_Identity=43.4108527131783, Blast_Score=124, Evalue=3e-30,
Organism=Escherichia coli, GI1790159, Length=171, Percent_Identity=36.8421052631579, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1786894, Length=145, Percent_Identity=35.1724137931034, Blast_Score=109, Evalue=1e-25,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YPQE_BACSU (P50829)

Other databases:

- EMBL:   L47838
- EMBL:   AL009126
- PIR:   H69940
- RefSeq:   NP_390105.1
- ProteinModelPortal:   P50829
- SMR:   P50829
- EnsemblBacteria:   EBBACT00000001812
- GeneID:   939046
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22230
- NMPDR:   fig|224308.1.peg.2228
- GenoList:   BSU22230
- GeneTree:   EBGT00050000000034
- HOGENOM:   HBG517865
- OMA:   EIILLEY
- PhylomeDB:   P50829
- ProtClustDB:   CLSK887487
- BioCyc:   BSUB:BSU22230-MONOMER
- GO:   GO:0005737
- GO:   GO:0016020
- InterPro:   IPR011055
- InterPro:   IPR001127
- TIGRFAMs:   TIGR00830

Pfam domain/function: PF00358 PTS_EIIA_1; SSF51261 Dup_hybrid_motif

EC number: 2.7.1.69

Molecular weight: Translated: 17936; Mature: 17936

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS51093 PTS_EIIA_TYPE_1; PS00371 PTS_EIIA_TYPE_1_HIS

Important sites: ACT_SITE 88-88

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLFGMGKIQEKVTEEVIYSPADGTVMDLSDVPDPVFSQKMMGEGIAVEPSSGEIVSP
CCHHHHCCCHHHHHHHHHHEECCCCCCEEECCCCCCHHHHHHHHCCCEEEECCCCCEECC
AEGEVIQIFHTKHAVGIRTRSGIELLIHVGLETVNMNGEGFTAHIKEGDKVKVGDPLITC
CCCCEEEEEECCEEEEEEECCCEEEEEEECEEEEEECCCCEEEEECCCCEEEECCCEEEE
DLELIKEKASSTVIPIVIMNGEAVGSMVSAGEKAARKGESKLFTIKAK
CHHHHHHHCCCEEEEEEEECCCHHHHHHHCCHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MLKKLFGMGKIQEKVTEEVIYSPADGTVMDLSDVPDPVFSQKMMGEGIAVEPSSGEIVSP
CCHHHHCCCHHHHHHHHHHEECCCCCCEEECCCCCCHHHHHHHHCCCEEEECCCCCEECC
AEGEVIQIFHTKHAVGIRTRSGIELLIHVGLETVNMNGEGFTAHIKEGDKVKVGDPLITC
CCCCEEEEEECCEEEEEEECCCEEEEEEECEEEEEECCCCEEEEECCCCEEEECCCEEEE
DLELIKEKASSTVIPIVIMNGEAVGSMVSAGEKAARKGESKLFTIKAK
CHHHHHHHCCCEEEEEEEECCCHHHHHHHCCHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377