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The map label for this gene is yojI

Identifier: 16079004

GI number: 16079004

Start: 2119393

End: 2120751

Strand: Direct

Name: yojI

Synonym: BSU19440

Alternate gene names: 16079004

Gene position: 2119393-2120751 (Clockwise)

Preceding gene: 16078995

Following gene: 16079005

Centisome position: 50.27

GC content: 44.89

Gene sequence:

>1359_bases
ATGAAAGAAACTCAAAACGCCCGGGAAAAAATCAAACAGCTCTTGCACATTTTAATACCGATATTCATTACCCAAGCGGG
GCTTTCTCTGATCACTTTTCTAGATACTGTGATGTCAGGGAAAGTCAGCCCCGCTGATTTAGCTGGTGTCGCCATTGGCT
CAAGCCTTTGGACCCCTGTTTACACAGGACTTGCAGGTATATTAATGGCGGTCACCCCGATTGTCGCTCAGTTATTAGGT
GCAGAGAAAAAACAGAAGATTCCCTTTACTGTTTTACAAGCTGTATACGTGGCCGCGCTTCTTAGCATCGCCGTTTTAGT
GATTGGATATGCTGCAGTTGACCTTATTCTTGGCCGATTGAACCTTGATATTCACGTGCATCAGATTGCTAAACACTTTT
TAGGCTTTTTATCACTGGGAATTTTCCCTTTATTTGTTTACACCGTGTTGAGATCATTCATCGACTCATTAGGAAAAACG
AGAGTCACCATGATGATTACACTCAGTTCTCTTCCAATCAATTTTGTATTGAATTATGTATTTATCTTCGGGAAGTTCGG
AATGCCGGCACTTGGAGGTGTAGGCGCGGGACTGGCATCCGCGTTAACTTACTGGTGTATATGCATCATCAGTTTTTTCA
TTATTCATAAAAACGCACCGTTTTCTGAATACGGGATTTTTCTTACCATGTATAAGTTCTCTTGGAAAGCATGCAAAAAT
TTGCTCAAAATCGGGCTGCCTATTGGGTTCGCCGTGTTTTTTGAAACGAGCATTTTCGCAGCTGTCACGCTTCTCATGAG
CCATTTTCATACAGTGACCATCGCGTCCCACCAGGCAGCCATGAATTTTGCGTCTCTTTTATATATGCTGCCGCTTAGCG
TGTCCATGGCTCTAACAATTGTTGTCGGATTTGAAGCGGGTGCAGCCCGTTTTAAGGATGCACGCTCATATAGCTTAATC
GGCATCATGATGGCGATCGGCTTTTCTTTGTTCACTGCCGCCTGTATCCTTTTATTCAGAGAGCAAATTGCGGGCATGTA
CACTTCTGACCCCGATGTGCTCCGGCTGACACAGCACTTTTTAATTTATGCGCTATTTTTTCAGCTTTCCGATGCTGTTG
CCGCACCGATCCAGGGAGCACTCAGAGGGTACAAAGATGTGAACTATACGCTTGCGGCCGCTTTTGTCTCTTACTGGGTC
ATTGGCCTTCCGGTCGGTTATATGGTCGGTACGTTTACAAGCCTCGGTGCATTCGGCTATTGGATTGGATTAATAGCGGG
ACTCGCCGCCGGAGCAGTTGGGCTGTTTTTCAGGCTGGCAAAACTGCAAAAACGTTATTCGCAAAAACAACACATTTAA

Upstream 100 bases:

>100_bases
AACGAAACCGACAAAAAAAGAATAGGCCCGGCGTTCATTGCTTTTTATACATAAAATGTTATAATTTCTCTCGTTATGAA
AAAGAAACGGAGATCAATGT

Downstream 100 bases:

>100_bases
AAGAGGTGATCAATCATGGCAAAAAACAAAAAATTATTCGAGTATCTTTCCCAGCATGCGGAAACCATCAGTTCAACATG
GTATGAAACAATCGAGGAGA

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKETQNAREKIKQLLHILIPIFITQAGLSLITFLDTVMSGKVSPADLAGVAIGSSLWTPVYTGLAGILMAVTPIVAQLLG
AEKKQKIPFTVLQAVYVAALLSIAVLVIGYAAVDLILGRLNLDIHVHQIAKHFLGFLSLGIFPLFVYTVLRSFIDSLGKT
RVTMMITLSSLPINFVLNYVFIFGKFGMPALGGVGAGLASALTYWCICIISFFIIHKNAPFSEYGIFLTMYKFSWKACKN
LLKIGLPIGFAVFFETSIFAAVTLLMSHFHTVTIASHQAAMNFASLLYMLPLSVSMALTIVVGFEAGAARFKDARSYSLI
GIMMAIGFSLFTAACILLFREQIAGMYTSDPDVLRLTQHFLIYALFFQLSDAVAAPIQGALRGYKDVNYTLAAAFVSYWV
IGLPVGYMVGTFTSLGAFGYWIGLIAGLAAGAVGLFFRLAKLQKRYSQKQHI

Sequences:

>Translated_452_residues
MKETQNAREKIKQLLHILIPIFITQAGLSLITFLDTVMSGKVSPADLAGVAIGSSLWTPVYTGLAGILMAVTPIVAQLLG
AEKKQKIPFTVLQAVYVAALLSIAVLVIGYAAVDLILGRLNLDIHVHQIAKHFLGFLSLGIFPLFVYTVLRSFIDSLGKT
RVTMMITLSSLPINFVLNYVFIFGKFGMPALGGVGAGLASALTYWCICIISFFIIHKNAPFSEYGIFLTMYKFSWKACKN
LLKIGLPIGFAVFFETSIFAAVTLLMSHFHTVTIASHQAAMNFASLLYMLPLSVSMALTIVVGFEAGAARFKDARSYSLI
GIMMAIGFSLFTAACILLFREQIAGMYTSDPDVLRLTQHFLIYALFFQLSDAVAAPIQGALRGYKDVNYTLAAAFVSYWV
IGLPVGYMVGTFTSLGAFGYWIGLIAGLAAGAVGLFFRLAKLQKRYSQKQHI
>Mature_452_residues
MKETQNAREKIKQLLHILIPIFITQAGLSLITFLDTVMSGKVSPADLAGVAIGSSLWTPVYTGLAGILMAVTPIVAQLLG
AEKKQKIPFTVLQAVYVAALLSIAVLVIGYAAVDLILGRLNLDIHVHQIAKHFLGFLSLGIFPLFVYTVLRSFIDSLGKT
RVTMMITLSSLPINFVLNYVFIFGKFGMPALGGVGAGLASALTYWCICIISFFIIHKNAPFSEYGIFLTMYKFSWKACKN
LLKIGLPIGFAVFFETSIFAAVTLLMSHFHTVTIASHQAAMNFASLLYMLPLSVSMALTIVVGFEAGAARFKDARSYSLI
GIMMAIGFSLFTAACILLFREQIAGMYTSDPDVLRLTQHFLIYALFFQLSDAVAAPIQGALRGYKDVNYTLAAAFVSYWV
IGLPVGYMVGTFTSLGAFGYWIGLIAGLAAGAVGLFFRLAKLQKRYSQKQHI

Specific function: Multidrug efflux pump

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Homologues:

Organism=Homo sapiens, GI153792564, Length=442, Percent_Identity=21.0407239819005, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI22907060, Length=427, Percent_Identity=22.0140515222482, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI38261960, Length=197, Percent_Identity=26.3959390862944, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI48994890, Length=444, Percent_Identity=34.9099099099099, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI226510954, Length=452, Percent_Identity=23.4513274336283, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321821, Length=376, Percent_Identity=24.2021276595745, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320545, Length=167, Percent_Identity=26.3473053892216, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NORM_BACSU (O31855)

Other databases:

- EMBL:   AF026147
- EMBL:   AL009126
- PIR:   F69906
- RefSeq:   NP_389826.1
- ProteinModelPortal:   O31855
- EnsemblBacteria:   EBBACT00000000868
- GeneID:   939592
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU19440
- NMPDR:   fig|224308.1.peg.1949
- GenoList:   BSU19440
- GeneTree:   EBGT00050000000379
- HOGENOM:   HBG479615
- OMA:   PINFVLN
- PhylomeDB:   O31855
- ProtClustDB:   PRK01766
- BioCyc:   BSUB:BSU19440-MONOMER
- InterPro:   IPR002528
- PANTHER:   PTHR11206
- TIGRFAMs:   TIGR00797

Pfam domain/function: PF01554 MatE

EC number: NA

Molecular weight: Translated: 49255; Mature: 49255

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xfe56ac8)-; HASH(0xcb2faf0)-; HASH(0xfb69c9c)-; HASH(0xf7e934c)-; HASH(0xfb55f10)-; HASH(0xe61905c)-; HASH(0xfb021d4)-; HASH(0xf18e4ec)-; HASH(0xfb023e4)-; HASH(0xb6a0514)-; HASH(0xfc9c9f8)-; HASH(0xf416c0c)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377