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The map label for this gene is pps

Identifier: 16078943

GI number: 16078943

Start: 2051329

End: 2053929

Strand: Reverse

Name: pps

Synonym: BSU18830

Alternate gene names: 16078943

Gene position: 2053929-2051329 (Counterclockwise)

Preceding gene: 16078944

Following gene: 16078941

Centisome position: 48.72

GC content: 41.68

Gene sequence:

>2601_bases
ATGAGTTCTTTGGTTCTCGGTTTACATGAAATTGAAAAAACACAGCTTTCGCTCGTTGGAGGAAAAGGGTTACATTTAGG
GGAATTATCAAAAATTCAAGGTATACAAGTACCAGAAGGATTTTGTGTCACAACAGTAGGATATCAAAAAGCCATCGAAC
AAAACGAAACGTTACAAGTGTTGTTGGATCAATTGACCATGCTAAAAGTTGAGGACCGAGATCAGATTGGTAACATCAGC
CGGAAGATTCGGCAAATCATTATGGAAGTAGACATTCCTTCCGATGTTGTGAAAGCAGTGGCTCAATATCTCTCCCAGTT
TGGCGAGGAACATGCCTATGCCGTGCGTTCTAGTGCGACTGCTGAGGATTTACCACATGCTTCTTTTGCCGGTCAACAAG
ACACCTATTTAAATATTACCGGCGTCGATGCAATCTTGCAGCATATCAGTAAATGCTGGGCGTCCCTATTTACGGATCGC
GCGGTTATTTACCGTATGCAAAATGGATTTGACCACAGCCAAGTCTATTTATCCGTTATCGTTCAAAGGATGGTTTTCCC
TCAGGCTTCGGGGATTTTATTTACCGCTGATCCGATTACCTCCAACCGAAAGGTGTTATCCATCGATGCCGGTTTTGGAC
TTGGTGAAGCACTGGTTTCTGGCTTGGTATCTGCCGATTGTTTTAAAGTACAGGATGGGCAAATCATCGATAAGAGAATA
GCAACTAAAAAAATGGCTATCTACGGACGAAAAGAAGGCGGAACAGAAACACAGCAGATCGATTCTGATCAGCAAAAGGC
TCAAACACTGACTGATGAACAAATTTTACAGCTGGCACGCATTGGAAGACAGATCGAAGCTCATTTCGGCCAGCCGCAAG
ATATCGAATGGTGTTTGGCCCGCGATACTTTTTATATTGTACAGAGTCGTCCAATCACTACTTTATTCCCCATCCCTGAA
GCGAGTGACCAAGAAAATCACGTCTATATTTCTGTTGGTCATCAACAAATGATGACGGATCCCATAAAACCACTGGGATT
GTCTTTTTTTCTGTTGACGACTGTTGCACCCATGCGTAAAGCCGGTGGAAGGCTATTTGTTGATGTCACACATCATCTGG
CATCCCCTGACAGCAGAGAAGTTTTCTTAAAAGGCATGGGGCAGCACGATCAGCTCTTAAAAGACGCACTTATGACCATA
ATAAAGCGACGAGATTTCATAAAGTCGATACCAAATGATAAAACAGCACCAAATCCCAGCAGAGGCAATGCAGATATGCC
GGCTCAAGTTGAAAACGATCCGACAATCGTGTCTGATTTGATTGAGAGCAGTCAAACATCGATAGAAGAGTTAAAACAAA
ACATCCAAACGAAATCAGGATCAGATTTATTTCGTTTTATTCTGGAAGATATTCAGGAATTAAAGAAGATTTTATTTAAC
CCAAAAAGTTCGGTTCTAATTCGAACTGCTATGAATGCTTCATTATGGATTAACGAAAAAATGAACGAGTGGTTAGGTGA
AAAAAACGCAGCAGATACGCTTTCTCAATCTGTACCCCATAATATTACTTCAGAAATGGGTCTTGCGCTATTAGACGTCG
CAGATGTGATTCGTCCTTATCCAGAGGTAATTGATTACTTACAACATGTAAAAGATGATAACTTTTTGGATGAACTGGCT
AAGTTTGATGGCGGAAGCAAAACCCGAGACGCGATCTATGATTATCTCAGCAAATACGGAATGCGATGTACCGGAGAGAT
TGATATCACAAGAACTCGATGGAGCGAAAAACCAACTACACTTGTCCCAATGATTCTTAATAATATTAAAAATTTTGAGC
CTAATGTCGGCCATCGGAAATTTGAGCAAGGGCGACAGGAAGCTTTGAAGAAAGAACAAGAGTTATTAGATCGATTGAAG
CAATTACCGGATGGTGAACAAAAAGCCAAAGAAACAAAACGAGCGATAGATATCATCCGGAATTTCAGCGGGTTTAGGGA
ATATCCAAAATACGGCATGGTTAATCGATACTTCGTTTATAAGCAGGCTTTACTGAAAGAAGCCGAACAACTCATAGAAG
CGGGCGTTATTCATGAAAAAGAAGATATATACTATCTCACTTTTGAAGAACTTCACGAAGTCGTGCGCACACATAAACTG
GATTATCAAATCATCAGTACACGAAAAGACGAGTACACATTATATGAAAAACTATCTCCACCACGTGTTATCACGTCTGA
TGGTGAAATCGTTACAGGTGAGTATAAACGAGAAAATCTCCCAGCCGGAGCGATTGTAGGTCTACCTGTTTCCTCCGGAG
TTATAGAGGGGCGAGCGCGTGTCATTTTAAACATGGAAGATGCGGATTTAGAAGATGGAGATATATTAGTTACCTCCTTT
ACCGATCCAAGCTGGACACCATTGTTTGTATCCATAAAAGGCCTAGTCACCGAAGTTGGGGGGCTGATGACCCATGGAGC
TGTTATAGCCCGTGAATATGGCTTACCTGCAGTTGTTGGCGTTGAAAATGCTGCCAAGCTGATAAAAGATGGGCAACGAA
TTCGCGTTCATGGTACAGAAGGCTACATTGAAATCTTCTAA

Upstream 100 bases:

>100_bases
GCGCTTTCTAGTTAAAAAGTTATAGATATCCATCTTATAATTTTATTATATCCTGCTAGGAAGCTCTTGTCAATATTTTT
TTTTGAAGGGGAGATGTAGA

Downstream 100 bases:

>100_bases
TGTCTGATGTGTGTAGAGAACCGTCAGATATTCCGCCATCAGCCCTAACTAGAAGTTGGGAGGCTGCATTTGTACCGAAT
GATAAAGTTCCCGTTGAACT

Product: phosphoenolpyruvate synthase

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase

Number of amino acids: Translated: 866; Mature: 865

Protein sequence:

>866_residues
MSSLVLGLHEIEKTQLSLVGGKGLHLGELSKIQGIQVPEGFCVTTVGYQKAIEQNETLQVLLDQLTMLKVEDRDQIGNIS
RKIRQIIMEVDIPSDVVKAVAQYLSQFGEEHAYAVRSSATAEDLPHASFAGQQDTYLNITGVDAILQHISKCWASLFTDR
AVIYRMQNGFDHSQVYLSVIVQRMVFPQASGILFTADPITSNRKVLSIDAGFGLGEALVSGLVSADCFKVQDGQIIDKRI
ATKKMAIYGRKEGGTETQQIDSDQQKAQTLTDEQILQLARIGRQIEAHFGQPQDIEWCLARDTFYIVQSRPITTLFPIPE
ASDQENHVYISVGHQQMMTDPIKPLGLSFFLLTTVAPMRKAGGRLFVDVTHHLASPDSREVFLKGMGQHDQLLKDALMTI
IKRRDFIKSIPNDKTAPNPSRGNADMPAQVENDPTIVSDLIESSQTSIEELKQNIQTKSGSDLFRFILEDIQELKKILFN
PKSSVLIRTAMNASLWINEKMNEWLGEKNAADTLSQSVPHNITSEMGLALLDVADVIRPYPEVIDYLQHVKDDNFLDELA
KFDGGSKTRDAIYDYLSKYGMRCTGEIDITRTRWSEKPTTLVPMILNNIKNFEPNVGHRKFEQGRQEALKKEQELLDRLK
QLPDGEQKAKETKRAIDIIRNFSGFREYPKYGMVNRYFVYKQALLKEAEQLIEAGVIHEKEDIYYLTFEELHEVVRTHKL
DYQIISTRKDEYTLYEKLSPPRVITSDGEIVTGEYKRENLPAGAIVGLPVSSGVIEGRARVILNMEDADLEDGDILVTSF
TDPSWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENAAKLIKDGQRIRVHGTEGYIEIF

Sequences:

>Translated_866_residues
MSSLVLGLHEIEKTQLSLVGGKGLHLGELSKIQGIQVPEGFCVTTVGYQKAIEQNETLQVLLDQLTMLKVEDRDQIGNIS
RKIRQIIMEVDIPSDVVKAVAQYLSQFGEEHAYAVRSSATAEDLPHASFAGQQDTYLNITGVDAILQHISKCWASLFTDR
AVIYRMQNGFDHSQVYLSVIVQRMVFPQASGILFTADPITSNRKVLSIDAGFGLGEALVSGLVSADCFKVQDGQIIDKRI
ATKKMAIYGRKEGGTETQQIDSDQQKAQTLTDEQILQLARIGRQIEAHFGQPQDIEWCLARDTFYIVQSRPITTLFPIPE
ASDQENHVYISVGHQQMMTDPIKPLGLSFFLLTTVAPMRKAGGRLFVDVTHHLASPDSREVFLKGMGQHDQLLKDALMTI
IKRRDFIKSIPNDKTAPNPSRGNADMPAQVENDPTIVSDLIESSQTSIEELKQNIQTKSGSDLFRFILEDIQELKKILFN
PKSSVLIRTAMNASLWINEKMNEWLGEKNAADTLSQSVPHNITSEMGLALLDVADVIRPYPEVIDYLQHVKDDNFLDELA
KFDGGSKTRDAIYDYLSKYGMRCTGEIDITRTRWSEKPTTLVPMILNNIKNFEPNVGHRKFEQGRQEALKKEQELLDRLK
QLPDGEQKAKETKRAIDIIRNFSGFREYPKYGMVNRYFVYKQALLKEAEQLIEAGVIHEKEDIYYLTFEELHEVVRTHKL
DYQIISTRKDEYTLYEKLSPPRVITSDGEIVTGEYKRENLPAGAIVGLPVSSGVIEGRARVILNMEDADLEDGDILVTSF
TDPSWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENAAKLIKDGQRIRVHGTEGYIEIF
>Mature_865_residues
SSLVLGLHEIEKTQLSLVGGKGLHLGELSKIQGIQVPEGFCVTTVGYQKAIEQNETLQVLLDQLTMLKVEDRDQIGNISR
KIRQIIMEVDIPSDVVKAVAQYLSQFGEEHAYAVRSSATAEDLPHASFAGQQDTYLNITGVDAILQHISKCWASLFTDRA
VIYRMQNGFDHSQVYLSVIVQRMVFPQASGILFTADPITSNRKVLSIDAGFGLGEALVSGLVSADCFKVQDGQIIDKRIA
TKKMAIYGRKEGGTETQQIDSDQQKAQTLTDEQILQLARIGRQIEAHFGQPQDIEWCLARDTFYIVQSRPITTLFPIPEA
SDQENHVYISVGHQQMMTDPIKPLGLSFFLLTTVAPMRKAGGRLFVDVTHHLASPDSREVFLKGMGQHDQLLKDALMTII
KRRDFIKSIPNDKTAPNPSRGNADMPAQVENDPTIVSDLIESSQTSIEELKQNIQTKSGSDLFRFILEDIQELKKILFNP
KSSVLIRTAMNASLWINEKMNEWLGEKNAADTLSQSVPHNITSEMGLALLDVADVIRPYPEVIDYLQHVKDDNFLDELAK
FDGGSKTRDAIYDYLSKYGMRCTGEIDITRTRWSEKPTTLVPMILNNIKNFEPNVGHRKFEQGRQEALKKEQELLDRLKQ
LPDGEQKAKETKRAIDIIRNFSGFREYPKYGMVNRYFVYKQALLKEAEQLIEAGVIHEKEDIYYLTFEELHEVVRTHKLD
YQIISTRKDEYTLYEKLSPPRVITSDGEIVTGEYKRENLPAGAIVGLPVSSGVIEGRARVILNMEDADLEDGDILVTSFT
DPSWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENAAKLIKDGQRIRVHGTEGYIEIF

Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential)

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1787994, Length=334, Percent_Identity=35.9281437125749, Blast_Score=192, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17564524, Length=318, Percent_Identity=29.874213836478, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPS_BACSU (O34309)

Other databases:

- EMBL:   AF027868
- EMBL:   AL009126
- EMBL:   Z34519
- PIR:   F69681
- RefSeq:   NP_389764.1
- ProteinModelPortal:   O34309
- SMR:   O34309
- EnsemblBacteria:   EBBACT00000000168
- GeneID:   939985
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU18830
- NMPDR:   fig|224308.1.peg.1886
- GenoList:   BSU18830
- GeneTree:   EBGT00050000001068
- HOGENOM:   HBG510686
- OMA:   DVIRPYP
- ProtClustDB:   PRK06241
- BioCyc:   BSUB:BSU18830-MONOMER
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.50.30.10

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N; SSF52009 PEP_mobile

EC number: 2.7.9.2

Molecular weight: Translated: 97193; Mature: 97062

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 825-825

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLVLGLHEIEKTQLSLVGGKGLHLGELSKIQGIQVPEGFCVTTVGYQKAIEQNETLQV
CCCHHHHHHHHHHHHHEEECCCCCCCCCHHHHCCCCCCCCCEEEECCHHHHHCCCHHHHH
LLDQLTMLKVEDRDQIGNISRKIRQIIMEVDIPSDVVKAVAQYLSQFGEEHAYAVRSSAT
HHHHHHHHEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCC
AEDLPHASFAGQQDTYLNITGVDAILQHISKCWASLFTDRAVIYRMQNGFDHSQVYLSVI
HHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCHHHEEHHHCCCCHHHHHHHHH
VQRMVFPQASGILFTADPITSNRKVLSIDAGFGLGEALVSGLVSADCFKVQDGQIIDKRI
HHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEECCCCHHHHHH
ATKKMAIYGRKEGGTETQQIDSDQQKAQTLTDEQILQLARIGRQIEAHFGQPQDIEWCLA
HHHHHHEEECCCCCCHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
RDTFYIVQSRPITTLFPIPEASDQENHVYISVGHQQMMTDPIKPLGLSFFLLTTVAPMRK
CCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHH
AGGRLFVDVTHHLASPDSREVFLKGMGQHDQLLKDALMTIIKRRDFIKSIPNDKTAPNPS
CCCEEEEEEHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RGNADMPAQVENDPTIVSDLIESSQTSIEELKQNIQTKSGSDLFRFILEDIQELKKILFN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
PKSSVLIRTAMNASLWINEKMNEWLGEKNAADTLSQSVPHNITSEMGLALLDVADVIRPY
CCCCEEEEEECCCEEEECHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
PEVIDYLQHVKDDNFLDELAKFDGGSKTRDAIYDYLSKYGMRCTGEIDITRTRWSEKPTT
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECEEEEEECCCCCCCCE
LVPMILNNIKNFEPNVGHRKFEQGRQEALKKEQELLDRLKQLPDGEQKAKETKRAIDIIR
EHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
NFSGFREYPKYGMVNRYFVYKQALLKEAEQLIEAGVIHEKEDIYYLTFEELHEVVRTHKL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCC
DYQIISTRKDEYTLYEKLSPPRVITSDGEIVTGEYKRENLPAGAIVGLPVSSGVIEGRAR
CEEEECCCCCCHHHHHHCCCCEEEECCCCEEECCCCCCCCCCCCEEECCCCCCEECCCEE
VILNMEDADLEDGDILVTSFTDPSWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVG
EEEEECCCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHCHHHHHHCCCCEEEE
VENAAKLIKDGQRIRVHGTEGYIEIF
HHHHHHHHCCCCEEEEECCCCEEEEC
>Mature Secondary Structure 
SSLVLGLHEIEKTQLSLVGGKGLHLGELSKIQGIQVPEGFCVTTVGYQKAIEQNETLQV
CCHHHHHHHHHHHHHEEECCCCCCCCCHHHHCCCCCCCCCEEEECCHHHHHCCCHHHHH
LLDQLTMLKVEDRDQIGNISRKIRQIIMEVDIPSDVVKAVAQYLSQFGEEHAYAVRSSAT
HHHHHHHHEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCC
AEDLPHASFAGQQDTYLNITGVDAILQHISKCWASLFTDRAVIYRMQNGFDHSQVYLSVI
HHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCHHHEEHHHCCCCHHHHHHHHH
VQRMVFPQASGILFTADPITSNRKVLSIDAGFGLGEALVSGLVSADCFKVQDGQIIDKRI
HHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEECCCCHHHHHH
ATKKMAIYGRKEGGTETQQIDSDQQKAQTLTDEQILQLARIGRQIEAHFGQPQDIEWCLA
HHHHHHEEECCCCCCHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
RDTFYIVQSRPITTLFPIPEASDQENHVYISVGHQQMMTDPIKPLGLSFFLLTTVAPMRK
CCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHH
AGGRLFVDVTHHLASPDSREVFLKGMGQHDQLLKDALMTIIKRRDFIKSIPNDKTAPNPS
CCCEEEEEEHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RGNADMPAQVENDPTIVSDLIESSQTSIEELKQNIQTKSGSDLFRFILEDIQELKKILFN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
PKSSVLIRTAMNASLWINEKMNEWLGEKNAADTLSQSVPHNITSEMGLALLDVADVIRPY
CCCCEEEEEECCCEEEECHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
PEVIDYLQHVKDDNFLDELAKFDGGSKTRDAIYDYLSKYGMRCTGEIDITRTRWSEKPTT
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECEEEEEECCCCCCCCE
LVPMILNNIKNFEPNVGHRKFEQGRQEALKKEQELLDRLKQLPDGEQKAKETKRAIDIIR
EHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
NFSGFREYPKYGMVNRYFVYKQALLKEAEQLIEAGVIHEKEDIYYLTFEELHEVVRTHKL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCC
DYQIISTRKDEYTLYEKLSPPRVITSDGEIVTGEYKRENLPAGAIVGLPVSSGVIEGRAR
CEEEECCCCCCHHHHHHCCCCEEEECCCCEEECCCCCCCCCCCCEEECCCCCCEECCCEE
VILNMEDADLEDGDILVTSFTDPSWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVG
EEEEECCCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHCHHHHHHCCCCEEEE
VENAAKLIKDGQRIRVHGTEGYIEIF
HHHHHHHHCCCCEEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.083 {pyruvate}} 10.5 {phosphate}} 0.028 {ATP}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7704256