Click here to switch to the map view.

The map label for this gene is alrB

Identifier: 16078827

GI number: 16078827

Start: 1897941

End: 1899125

Strand: Reverse

Name: alrB

Synonym: BSU17640

Alternate gene names: 16078827

Gene position: 1899125-1897941 (Counterclockwise)

Preceding gene: 16078830

Following gene: 16078825

Centisome position: 45.05

GC content: 43.88

Gene sequence:

>1185_bases
ATGATAAAGCTTTGCCGAGAAGTTTGGATCGAAGTCAATCTTGATGCCGTTAAAAAGAATTTGCGAGCGATCCGCCGTCA
TATTCCACATAAGAGCAAAATTATGGCTGTTGTAAAAGCGAACGGTTATGGCCACGGGTCTATAGAAGTGGCACGCCATG
CACTGGAACACGGGGCGAGTGAGCTCGCTGTTGCCAGTGTGGAGGAAGGAATCGTTTTACGTAAAGCTGGGATTACAGCA
CCTATCCTTGTGCTTGGTTTTACATCCCTTAGTTGTGTAAAGAAGTCAGCAGCTTGGAATATAACATTATCAGCATTTCA
GGTTGATTGGATGAAAGAAGCAAACGAGATATTGGAAAAAGAAGCGAGTGCTAACCGGCTGGCCATTCATATTAACGTGG
ATACCGGCATGGGACGATTAGGTGTACGCACAAAGGAAGAACTTTTAGAGGTAGTGAAAGCCTTAAAGGCAAGTAAATTC
CTCAGATGGACAGGGATTTTCACACATTTTTCGACAGCTGATGAACCGGACACAACACTCACAAAGCTGCAGCATGAAAA
ATTCATCAGTTTTCTCAGCTTTTTAAAAAAACAGGGGATTGAGCTGCCCACCGTGCATATGTGCAACACGGCTGCAGCTA
TCGCATTTCCGGAATTTAGCGCTGATATGATCCGTTTAGGTATCGGCTTATATGGTTTATATCCTTCAGCCTATATTAAA
CAACTAAATCTCGTTAAGCTTGAACCTGCGTTAAGTTTAAAGGCACGCATCGCTTATGTGAAAACCATGCGGACAGAACC
CCGAACCGTTAGCTATGGTGCTACATACATCGCAGAACCTAATGAAGTAATTGCTACACTCCCGATTGGCTATGCTGACG
GCTATTCTCGTGCCCTTTCCAATCGCGGGTTTGTTCTTCATCGCGGAAAACGAGTGCCAGTGGCGGGAAGAGTAACAATG
GATATGATCATGGTCAGTTTAGGAGAAAACGGTGAAGGTAAACAAGGAGATGAAGTCGTGATTTACGGTAAGCAAAAAGG
AGCCGAGATTTCCGTTGATGAAGTGGCTGAAATGCTCAATACGATTAACTATGAAGTCGTGTCTACATTAAGCCGCCGCA
TCCCTCGTTTTTATATAAGAGATGGCGAGATTTTTAAAGTATCAACTCCAGTATTATACGTGTAG

Upstream 100 bases:

>100_bases
AATAAAGTTGAATAAAATAAGATCGAATCATCCAACAATTAACATTTATTCTTGCGGCAATAGGTGGTATTTATAATCCA
TAAGAAGGAAGGAAGCGTTG

Downstream 100 bases:

>100_bases
AACATCTTAAAATGCTGAATCAAAATCCCTCTACCAGAGGGATTTTGTTTTTTCTGAAACTAAAAAAATCCCTCCTATAA
GAAGAGAGATTTTCAACATA

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MIKLCREVWIEVNLDAVKKNLRAIRRHIPHKSKIMAVVKANGYGHGSIEVARHALEHGASELAVASVEEGIVLRKAGITA
PILVLGFTSLSCVKKSAAWNITLSAFQVDWMKEANEILEKEASANRLAIHINVDTGMGRLGVRTKEELLEVVKALKASKF
LRWTGIFTHFSTADEPDTTLTKLQHEKFISFLSFLKKQGIELPTVHMCNTAAAIAFPEFSADMIRLGIGLYGLYPSAYIK
QLNLVKLEPALSLKARIAYVKTMRTEPRTVSYGATYIAEPNEVIATLPIGYADGYSRALSNRGFVLHRGKRVPVAGRVTM
DMIMVSLGENGEGKQGDEVVIYGKQKGAEISVDEVAEMLNTINYEVVSTLSRRIPRFYIRDGEIFKVSTPVLYV

Sequences:

>Translated_394_residues
MIKLCREVWIEVNLDAVKKNLRAIRRHIPHKSKIMAVVKANGYGHGSIEVARHALEHGASELAVASVEEGIVLRKAGITA
PILVLGFTSLSCVKKSAAWNITLSAFQVDWMKEANEILEKEASANRLAIHINVDTGMGRLGVRTKEELLEVVKALKASKF
LRWTGIFTHFSTADEPDTTLTKLQHEKFISFLSFLKKQGIELPTVHMCNTAAAIAFPEFSADMIRLGIGLYGLYPSAYIK
QLNLVKLEPALSLKARIAYVKTMRTEPRTVSYGATYIAEPNEVIATLPIGYADGYSRALSNRGFVLHRGKRVPVAGRVTM
DMIMVSLGENGEGKQGDEVVIYGKQKGAEISVDEVAEMLNTINYEVVSTLSRRIPRFYIRDGEIFKVSTPVLYV
>Mature_394_residues
MIKLCREVWIEVNLDAVKKNLRAIRRHIPHKSKIMAVVKANGYGHGSIEVARHALEHGASELAVASVEEGIVLRKAGITA
PILVLGFTSLSCVKKSAAWNITLSAFQVDWMKEANEILEKEASANRLAIHINVDTGMGRLGVRTKEELLEVVKALKASKF
LRWTGIFTHFSTADEPDTTLTKLQHEKFISFLSFLKKQGIELPTVHMCNTAAAIAFPEFSADMIRLGIGLYGLYPSAYIK
QLNLVKLEPALSLKARIAYVKTMRTEPRTVSYGATYIAEPNEVIATLPIGYADGYSRALSNRGFVLHRGKRVPVAGRVTM
DMIMVSLGENGEGKQGDEVVIYGKQKGAEISVDEVAEMLNTINYEVVSTLSRRIPRFYIRDGEIFKVSTPVLYV

Specific function: Provides the D-alanine required for cell wall biosynthesis

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family

Homologues:

Organism=Escherichia coli, GI1790487, Length=370, Percent_Identity=31.6216216216216, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1787439, Length=377, Percent_Identity=31.2997347480106, Blast_Score=152, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALR2_BACSU (P94494)

Other databases:

- EMBL:   U66480
- EMBL:   AL009126
- PIR:   F69888
- RefSeq:   NP_389646.1
- ProteinModelPortal:   P94494
- SMR:   P94494
- EnsemblBacteria:   EBBACT00000002352
- GeneID:   939550
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17640
- NMPDR:   fig|224308.1.peg.1768
- GenoList:   BSU17640
- GeneTree:   EBGT00050000001615
- HOGENOM:   HBG712172
- OMA:   YGGEAVN
- PhylomeDB:   P94494
- ProtClustDB:   CLSK887341
- BioCyc:   BSUB:BSU17640-MONOMER
- BRENDA:   5.1.1.1
- HAMAP:   MF_01201
- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622
- Gene3D:   G3DSA:2.40.37.10
- PRINTS:   PR00992
- TIGRFAMs:   TIGR00492

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N; SSF50621 Racem_decarbox_C

EC number: =5.1.1.1

Molecular weight: Translated: 43648; Mature: 43648

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: ACT_SITE 39-39 ACT_SITE 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLCREVWIEVNLDAVKKNLRAIRRHIPHKSKIMAVVKANGYGHGSIEVARHALEHGAS
CCCEEEEHHEECCHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHHHHHHCCHH
ELAVASVEEGIVLRKAGITAPILVLGFTSLSCVKKSAAWNITLSAFQVDWMKEANEILEK
HHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHH
EASANRLAIHINVDTGMGRLGVRTKEELLEVVKALKASKFLRWTGIFTHFSTADEPDTTL
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHH
TKLQHEKFISFLSFLKKQGIELPTVHMCNTAAAIAFPEFSADMIRLGIGLYGLYPSAYIK
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHCCHHHHHH
QLNLVKLEPALSLKARIAYVKTMRTEPRTVSYGATYIAEPNEVIATLPIGYADGYSRALS
CCCEEEECCCHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCEEEEECCCCCCCHHHHHC
NRGFVLHRGKRVPVAGRVTMDMIMVSLGENGEGKQGDEVVIYGKQKGAEISVDEVAEMLN
CCCEEEECCCCCCEECHHHHHHHHEECCCCCCCCCCCEEEEEECCCCCEECHHHHHHHHH
TINYEVVSTLSRRIPRFYIRDGEIFKVSTPVLYV
HCCHHHHHHHHHCCCEEEEECCCEEEECCCEEEC
>Mature Secondary Structure
MIKLCREVWIEVNLDAVKKNLRAIRRHIPHKSKIMAVVKANGYGHGSIEVARHALEHGAS
CCCEEEEHHEECCHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHHHHHHCCHH
ELAVASVEEGIVLRKAGITAPILVLGFTSLSCVKKSAAWNITLSAFQVDWMKEANEILEK
HHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHH
EASANRLAIHINVDTGMGRLGVRTKEELLEVVKALKASKFLRWTGIFTHFSTADEPDTTL
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHH
TKLQHEKFISFLSFLKKQGIELPTVHMCNTAAAIAFPEFSADMIRLGIGLYGLYPSAYIK
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHCCHHHHHH
QLNLVKLEPALSLKARIAYVKTMRTEPRTVSYGATYIAEPNEVIATLPIGYADGYSRALS
CCCEEEECCCHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCEEEEECCCCCCCHHHHHC
NRGFVLHRGKRVPVAGRVTMDMIMVSLGENGEGKQGDEVVIYGKQKGAEISVDEVAEMLN
CCCEEEECCCCCCEECHHHHHHHHEECCCCCCCCCCCEEEEEECCCCCEECHHHHHHHHH
TINYEVVSTLSRRIPRFYIRDGEIFKVSTPVLYV
HCCHHHHHHHHHCCCEEEEECCCEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377